LOCUS       pNTI751_p(PGK1)_GCN4_5 10429 bp ds-DNA     circular     25-DEC-2020
DEFINITION  .
KEYWORDS    "marker:AmpR, HygR" "ref:pmid\:26376869"                           
FEATURES             Location/Qualifiers
     CDS             4729..5757
                     /label="HygR"
     primer_bind     complement(782..805)
                     /label="NI566"
     promoter        complement(6100..6118)
                     /label="T7 promoter"
     CDS             complement(9216..9284)
                     /label="AmpR"
     CDS             complement(8424..9215)
                     /label="AmpR"
     rep_origin      complement(7712..8300)
                     /label="ori"
     misc_feature    1201..1212
                     /label="uORF1"
     protein_bind    complement(4297..4330)
                     /label="loxP"
     misc_feature    9741..10196
                     /label="homology arm"
     misc_feature    2831..3712
                     /label="MSN2 transcriptional activator"
     terminator      30..219
                     /label="CYC1 terminator"
     protein_bind    complement(6923..6956)
                     /label="FRT (minimal)"
     promoter        4385..4728
                     /label="TEF promoter"
     misc_feature    1411..1422
                     /label="uORF4"
     CDS             1907..2797
                     /label="ER-LBD"
     protein_bind    9669..9702
                     /label="FRT (minimal)"
     misc_feature    1562..3721
                     /label="synthetic transcription factor fusion"
     misc_feature    992..1561
                     /label="GCN4 5' UTR"
     misc_feature    1562..1819
                     /label="KROX-24 Zinc-Finger domain"
     promoter        236..991
                     /label="P(PGK1)"
     misc_feature    1269..1277
                     /label="uORF2"
     promoter        complement(9285..9376)
                     /label="AmpR promoter"
     misc_feature    3723..4187
                     /label="C. albicans TadH1 terminator"
     primer_bind     803..826
                     /label="NI565"
     primer_bind     complement(6128..6144)
                     /label="M13 fwd"
     gene            4385..5960
                     /label="hphMX6"
     misc_feature    1386..1397
                     /label="uORF3"
     protein_bind    complement(6023..6056)
                     /label="loxP"
     primer_bind     complement(7426..7442)
                     /label="M13 fwd"
     terminator      10235..10400
                     /label="ADH1 terminator"
     misc_feature    6205..6880
                     /label="homology arm"
     terminator      5763..5960
                     /label="TEF terminator"
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagGACTT
      241 CAACTCAAGA CGCACAGATA TTATAACATC TGCATAATAG GCATTTGCAA GAATTACTCG
      301 TGAGTAAGGA AAGAGTGAGG AACTATCGCA TACCTGCATT TAAAGATGCC GATTTGGGCG
      361 CGAATCCTTT ATTTTGGCTT CACCCTCATA CTATTATCAG GGCCAGAAAA AGGAAGTGTT
      421 TCCCTCCTTC TTGAATTGAT GTTACCCTCA TAAAGCACGT GGCCTCTTAT CGAGAAAGAA
      481 ATTACCGTCG CTCGTGATTT GTTTGCAAAA AGAACAAAAC TGAAAAAACC CAGACACGCT
      541 CGACTTCCTG TCTTCCTATT GATTGCAGCT TCCAATTTCG TCACACAACA AGGTCCTAGC
      601 GACGGCTCAC AGGTTTTGTA ACAAGCAATC GAAGGTTCTG GAATGGCGGG AAAGGGTTTA
      661 GTACCACATG CTATGATGCC CACTGTGATC TCCAGAGCAA AGTTCGTTCG ATCGTACTGT
      721 TACTCTCTCT CTTTCAAACA GAATTGTCCG AATCGTGTGA CAACAACAGC CTGTTCTCAC
      781 ACACTCTTTT CTTCTAACCA AGGGGGTGGT TTAGTTTAGT AGAACCTCGT GAAACTTACA
      841 TTTACATATA TATAAACTTG CATAAATTGG TCAATGCAAG AAATACATAT TTGGTCTTTT
      901 CTAATTCGTA GTTTTTCAAG TTCTTAGATG CTTTCTTTTT CTCTTTTTTA CAGATCATCA
      961 AGGAAGTAAT TATCTACTTT TTACAACAAA TAAACAAAAC AAAACAAAAA CTCACAACAC
     1021 AGGTTACTCT CCCCCCTAAA TTCAAATTTT TTTTGCCCAT CAGTTTCACT AGCGAATTAT
     1081 ACAACTCACC AGCCACACAG CTCACTCATC TACTTCGCAA TCAAAACAAA ATATTTTATT
     1141 TTAGTTCAGT TTATTAAGTT ATTATCAGTA TCGTATTAAA AAATTAAAGA TCATTGAAAA
     1201 ATGGCTTGCT AAACCGATTA TATTTTGTTT TTAAAGTAGA TTATTATTAG AAAATTATTA
     1261 AGAGAATTAT GTGTTAAATT TATTGAAAGA GAAAATTTAT TTTCCCTTAT TAATTAAAGT
     1321 CCTTTACTTT TTTTGAAAAC TGTCAGTTTT TTGAAGAGTT ATTTGTTTTG TTACCAATTG
     1381 CTATCATGTA CCCGTAGAAT TTTATTCAAG ATGTTTCCGT AACGGTTACC TTTCTGTCAA
     1441 ATTATCCAGG TTTACTCGCC AATAAAAATT TCCCTATACT ATCATTAATT AAATCATTAT
     1501 TATTACTAAA GTTTTGTTTA CCAATTTGTC TGCTCAAGAA AATAAATTAA ATACAAATAA
     1561 Aatgggtacc cgcccatatg cttgccctgt cgagtcctgc gatcgccgct tttctcgctc
     1621 ggatgagctt acccgccata tccgcatcca taccggtcag aagcccttcc agtgtcgaat
     1681 ctgcatgcgt aacttcagtc gtagtgacca ccttaccacc cacatccgca cccacacagg
     1741 cgagaagcct tttgcctgtg acatttgtgg gaggaagttt gccaggagtg atgaacgcaa
     1801 gaggcatacc aaaatccata caggtagatc cggtgacggt gctggtttaa ttaactctgc
     1861 tggagacatg agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa
     1921 gaacagcctg gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga
     1981 gccccccata ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat
     2041 gggcttactg accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag
     2101 ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg
     2161 gctagagatc ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact
     2221 gtttgctcct aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga
     2281 gatcttcgac atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga
     2341 ggagtttgtg tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc
     2401 cagcaccctg aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac
     2461 agacactttg atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg
     2521 gctggcccag ctcctcctca tcctctccca catcaggcac atgagtaaca aaggcatgga
     2581 gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggagat
     2641 gctggacgcc caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac
     2701 ggaccaaagc cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta
     2761 catcacgggg gaggcagagg gtttccctgc cacagtcgcc gcagcaggtg acggtgctgg
     2821 tttaattaac atgacggtcg accatgattt caatagcgaa gatattttat tccccataga
     2881 aagcatgagt agtatacaat acgtggagaa taataaccca aataatatta acaacgatgt
     2941 tatcccgtat tctctagata tcaaaaacac tgtcttagat agtgcggatc tcaatgacat
     3001 tcaaaatcaa gaaacttcac tgaatttggg gcttcctcca ctatctttcg actctccact
     3061 gcccgtaacg gaaacgatac catccactac cgataacagc ttgcatttga aagctgatag
     3121 caacaaaaat cgcgatgcaa gaactattga aaatgatagt gaaattaaga gtactaataa
     3181 tgctagtggc tctggggcaa atcaatacac aactcttact tcaccttatc ctatgaacga
     3241 cattttgtac aacatgaaca atccgttaca atcaccgtca ccttcatcgg tacctcaaaa
     3301 tccgactata aatcctccca taaatacagc aagtaacgaa actaatttat cgcctcaaac
     3361 ttcaaatggt aatgaaactc ttatatctcc tcgagcccaa caacatacgt ccattaaaga
     3421 taatcgtctg tccttaccta atggtgctaa ttcgaatctt ttcattgaca ctaacccaaa
     3481 caatttgaac gaaaaactaa gaaatcaatt gaactcagat acaaattcat attctaactc
     3541 catttctaat tcaaactcca attctacggg taatttaaat tccagttatt ttaattcact
     3601 gaacatagac tccatgctag atgattacgt ttctagtgat ctcttattga atgatgatga
     3661 tgatgacact aatttatcac gccgaagatt tagcgacgtt ataacaaacc aagagctcta
     3721 agcaaatagc taaattatat acgaattaat attatgatta agtgtttacg tgagtgcgat
     3781 atttttatta ctatcttata cagttgtata tactctataa aatgagttgt ctattaatta
     3841 acgcgatgaa tgctttctgg gtttacctct ccaacaactc tagtttactt ctcaatacat
     3901 tcaattgtat ttgatttgtc aatacttcat cattaatcaa ttctatagtt ttgtttttct
     3961 cgtttatttc caaatttaat gcatcaattt tattattcaa tttgtcgttg attttggtta
     4021 atgattttat ggtttgatct ctggcattga ttgtttgtgt tagtttttca ttattgataa
     4081 ttaaattatt taagttagtt atcaactcgg tgttttcaag tttcaagttt tcaatttctt
     4141 tagagtttat tagatttgtc aaagtttctg aattgcttga ttggtccggc ccaggtattg
     4201 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     4261 ggatccttcg tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat
     4321 acgaagttat taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc
     4381 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
     4441 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
     4501 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg
     4561 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
     4621 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
     4681 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaaaag
     4741 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     4801 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     4861 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     4921 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     4981 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     5041 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggcaatgga tgccattgct
     5101 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     5161 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     5221 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     5281 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     5341 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     5401 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     5461 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     5521 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     5581 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     5641 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     5701 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataatca
     5761 gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata
     5821 ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca
     5881 tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt
     5941 gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa
     6001 cgagctctcg agaaccctta atataacttc gtataatgta tgctatacga agttattagg
     6061 tgatatcaga tccactagtg gcctatgcac ccaattcgcc ctatagtgag tcgtattacg
     6121 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccctg
     6181 caggactagt gctgaggcat taatgcaact cagaagtttg acagcaagca agttcatcat
     6241 tcgaactagc cttattgttt tagttcagtg acagcgaact gccgtactcg atgctttatt
     6301 tctcacggta gagcggaaga acagataggg gcagcgtgag aagagttaga aagtaaattt
     6361 ttatcacgtc tgaagtattc ttattcatag gaaattttgc aaggtttttt agctcaataa
     6421 cgggctaagt tatataaggt gttcacgcga ttttcttgtt atgtatacct cttctctgag
     6481 gaatggtact actgtcctga tgtaggctcc ttaaattggt gggcaagaat aacttatcga
     6541 tattttgtat attggtcttg gagttcacca cgtaatgcct gtttaagacc atcagttaac
     6601 tctagtatta tttggtcttg gctactggcc gtttgctatt attcaagtct tttgtgcctt
     6661 cccgtcgggt aagggagtta tttagggata cagaatctaa cgaaaactaa atctcaatga
     6721 ttaactctat ttaatccttt tttgaaaggc aaaagaggtc ccttgttcac ttacaacgtt
     6781 cttagccaaa ttcgcttatc acttactact tcacgatata cagaagtaaa aacatataaa
     6841 aagatgtctg tttgtttagc catcacaaaa ggtatcgcag attaagacct cagcgcggcc
     6901 gcaaatttaa ataaaatgaa gtgaagttcc tatactttct agagaatagg aacttctata
     6961 gtgagtcgaa taagggcgac acaaaattta ttctaaatgc ataataaata ctgataacat
     7021 cttatagttt gtattatatt ttgtattatc gttgacatgt ataattttga tatcaaaaac
     7081 tgattttccc tttattattt tcgagattta ttttcttaat tctctttaac aaactagaaa
     7141 tattgtatat acaaaaaatc ataaataata gatgaatagt ttaattatag gtgttcatca
     7201 atcgaaaaag caacgtatct tatttaaagt gcgttgcttt tttctcattt ataaggttaa
     7261 ataattctca tatatcaagc aaagtgacag gcgcccttaa atattctgac aaatgctctt
     7321 tccctaaact ccccccataa aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt
     7381 aacgattact cgttatcaga accgcccagg gggcccgagc ttaagactgg ccgtcgtttt
     7441 acaacacaga aagagtttgt agaaacgcaa aaaggccatc cgtcaggggc cttctgctta
     7501 gtttgatgcc tggcagttcc ctactctcgc cttccgcttc ctcgctcact gactcgctgc
     7561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8101 gcggtgctac agagttcttg aagtggtggg ctaactacgg ctacactaga agaacagtat
     8161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8341 ggaacgacgc gcgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt
     8401 caagtcagcg taatgctctg cttttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8461 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8521 cgggagggct taccatctgg ccccagcgct gcgatgatac cgcgagaacc acgctcaccg
     8581 gctccggatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8641 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8701 tcgccagtta atagtttgcg caacgttgtt gccatcgcta caggcatcgt ggtgtcacgc
     8761 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8821 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8881 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8941 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9001 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9061 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatattctt cctttttcaa
     9301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9361 tagaaaaata aacaaatagg ggtcagtgtt acaaccaatt aaccaattct gaacattatc
     9421 gcgagcccat ttatacctga atatggctca taacacccct tgtttgcctg gcggcagtag
     9481 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
     9541 tagtgtgggg actccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     9601 ctcagtcgaa agactgggcc tttcgcccgg gctaattatg gggtgtcgcc cttattcgac
     9661 tctatagtga agttcctatt ctctagaaag tataggaact tctgaagtgg ggatttaaat
     9721 gcggccgcgc tgagggttta atcaatctgg cggcttgagt tctcaacatg ttttattttt
     9781 tacttatatt gctggtaggg taaaaaaata taactcctag gaataggttg tctatatgtt
     9841 tttgtcttgc ttctataatt gtaacaaaca aggaaaggga aaatactggg tgtaaaagcc
     9901 attgagtcaa gttaggtcat cccttttata caaaattttt caattttttt tccaagattc
     9961 ttgtacgatt aattattttt tttttgcgtc ctacagcgtg atgaaaattt cgcctgctgc
    10021 aagatgagcg ggaacgggcg aaatgtgcac gcgcacaact tacgaaacgc ggatgagtca
    10081 ctgacagcca ccgcagaggt tctgactcct actgagctct attggaggtg gcagaaccgg
    10141 taccggagga ggccgctata accggtttga atttattgtc acagtgtcac atcagcatta
    10201 agtcctcagc gagctcgcat ggaatgcgtg cgatgagcga cctcatgcta tacctgagaa
    10261 agcaacctga cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa
    10321 gagtcacttt aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc
    10381 ataaatcata agaaattcgc ttatttagaa gtgtcaacaa cgtatctac
//