LOCUS       Exported                4085 bp ds-DNA     circular SYN 30-DEC-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 30, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(234..612)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(793..897)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1371..1387
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1394..1412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           1444..1486
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      1533..1542
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1539..2552
                     /codon_start=1
                     /label=POLArISact
                     /label=POLArIS actin L5
                     /translation="MGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTTGKL
                     PVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVV
                     KFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFTVRHNV
                     EDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHEYVNAAGIT
                     LGMDELYKGGGSSKGEELFEAAAKASLEIEELARFAVDEHNKKENALLEFVRVVKAKEQ
                     SSVPHWWWTTMYYLTLEAKDGGKKKLYEAKVWVKRDPNMIFKINFKELQEFKPVGDA"
     3'UTR           2591..2716
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene 
                     ofXenopuslaevis"
     promoter        complement(2821..2839)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2857..2873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2881..2897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operatortoinhibit
                     transcription in E. coli. This inhibition can be relieved 
                     by adding lactose or isopropyl-beta-D-thiogalactopyranoside
                     (IPTG)."
     promoter        complement(2905..2935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2950..2971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in thepresenceof
                     cAMP."
     rep_origin      complement(3259..3848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
       61 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      121 gcccggaagg gccgagcgca gaagtggtcc tgcaaacttt atccgcctcc atccagtcta
      181 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      241 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
      301 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
      361 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
      421 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
      481 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
      541 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
      601 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
      661 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
      721 ctgggtgagc aaacaggaag gcaaatgccg aaaaaaggga ataagggcga cacggaaatg
      781 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      901 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
      961 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     1021 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     1081 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     1141 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     1201 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     1261 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     1321 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     1381 ggccagtgaa ttgtaatacg actcactata gggcgaatta attcgagctc ggtacccagc
     1441 ttgcttgttc tttttgcaga agctcagaat aaacgctcaa ctttggcaga tcaattcccc
     1501 ggtgctagcg ctaccggtct cagatctcga gtgccaccat gggagtcgta ccaatacttg
     1561 tggagttgga cggagatgtg aacggacaca aattttcagt ccgcggggag ggggaagggg
     1621 atgctactat tggcaagctg acgctcaaat tcatctgtac caccggaaaa ctccctgtac
     1681 cctggcccac actggtgaca actctgactt acggcgtgca atgttttagc cgatacccag
     1741 accacatgaa gaggcacgac tttttcaaaa gcgcaatgcc tgaaggatac gtacaggaaa
     1801 ggaccatttc ttttaaagac gacgggaagt acaaaacccg ggcagtggtg aagtttgagg
     1861 gcgataccct cgtcaatagg atcgaattga agggaactga cttcaaagaa gatggcaaca
     1921 tcctgggtca caagctcgag tataacttta actcccacaa cgtgtatatt acagccgaca
     1981 aacagaagaa tggaattaag gctaacttca ctgtcagaca caatgtcgaa gatggctccg
     2041 tgcagctcgc cgatcactat caacagaata ctcctatcgg ggacggccca gtcctgctgc
     2101 ccgacaacca ctacctgagt acccagactg ttctgagcaa agatccgaac gagaagcgcg
     2161 accacatggt gctgcatgag tatgtcaacg ctgcaggaat taccctcggc atggacgagc
     2221 tgtacaaggg cggtggatcc tccaaaggag aagagctgtt cgaggccgct gccaaggctt
     2281 ccctggagat cgaagaactg gctcggttcg ctgttgacga acacaacaaa aaagaaaacg
     2341 ctcttctaga attcgttcgc gttgttaaag ctaaagaaca gagcagcgtg ccccactggt
     2401 ggtggaccac catgtactac ctgaccctgg aagctaaaga cggtggtaaa aagaaactgt
     2461 acgaagctaa agtttgggtt aaacgcgacc ccaacatgat cttcaagatc aacttcaaag
     2521 aactgcagga gttcaaaccg gttggtgacg cttaatctag ataaatgata gcttgatctg
     2581 gttaccacta aaccagcctc aagaacaccc gaatggagtc tctaagctac ataataccaa
     2641 cttacacttt acaaaatgtt gtcccccaaa atgtagccat tcgtatctgc tcctaataaa
     2701 aagaaagttt cttcacattc taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaccccc
     2761 cccccccccc cctgcaggcg cgccgccggc gatttaaatt aattaatcta gcttgagtat
     2821 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     2881 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2941 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3001 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3061 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg accttatcgc
     3601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4081 ttgcc
//
