LOCUS       Exported                6120 bp ds-DNA     circular SYN 28-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    PE 21  KFF KFF KFF k  gfp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   renjun
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 4, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     25..46
                     /label=T7_ter_avrII[JMK,459]
     terminator      26..73
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..86
                     /label=T7ter[������,750]
     primer_bind     69..86
                     /label=T7ter[univeralprimer,101]
     primer_bind     69..86
                     /label=T7_terminator[SMH-seq,180]
     primer_bind     136..159
                     /label=YPK1_R_SalI[LHM-kinase,527]
     primer_bind     137..159
                     /label=Orm1_R_NcoI[LHM-kinase,529]
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    158
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    164..877
                     
     primer_bind     164..185
                     /label=17-GFP-R[������,931]
     primer_bind     164..184
                     /label=gfp_tomato_R_AfIII[SMH-etc.,411]
     primer_bind     164..183
                     /label=GFP_RT_PCR_R[SMH-seq,265]
     primer_bind     164..183
                     /label=gfp_e_R[������,858]
     primer_bind     164..183
                     /label=mcherryR_afIII[SMH-etc.,405]
     primer_bind     164..182
                     /label=UTR(AU)-gfp-R(BamHI)[������,942]
     primer_bind     164..182
                     /label=UTR(AU)-gfp-R(BamHI)
     primer_bind     164..182
                     /label=gfp_pr_R[SMH-etc.,166]
     primer_bind     164..179
                     /label=mcherry_R_BamHI[SMH-etc.,409]
     primer_bind     175..191
                     /label=EGFP-CR[univeralprimer,51]
     primer_bind     complement(254..274)
                     /label=EGFP-CF[univeralprimer,50]
     primer_bind     complement(270..289)
                     /label=GFP_RT_PCR_F[SMH-seq,264]
     primer_bind     complement(391..413)
                     /label=DH-1_LTL_conf_F1[LHM-LTL,627]
     primer_bind     complement(391..413)
                     /label=DH-1 LTL conf-F1[������,982]
     primer_bind     814..829
                     /label=EGFP-NR[univeralprimer,52]
     primer_bind     complement(858..877)
                     /label=16-GFP-F[������,930]
     primer_bind     complement(860..877)
                     /label=tomato_to_NewF[JMK,26]
     primer_bind     complement(860..877)
                     /label=gfp_tomato_F_NdeI[SMH-etc.,417]
     primer_bind     complement(860..877)
                     /label=Gfp_F_afIII[SMH-etc.,416]
     primer_bind     complement(861..877)
                     /label=NdeI_gfp[SMH-HFQ,232]
     primer_bind     complement(863..877)
                     /label=GFP_X_F[SMH-etc.,177]
     primer_bind     complement(866..883)
                     /label=Primer 3
     primer_bind     complement(866..883)
                     /label=Primer 1
     misc_feature    884..913
                     /label=cpp(Tat48-57)
     primer_bind     910..939
                     /label=Primer 2
     primer_bind     914..938
                     /label=vitro-invR-txb[N34][������,754]
     CDS             complement(916)
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /label=T7 tag
                     /translation=""
     RBS             925..930
                     /note="ribosome binding site"
     protein_bind    961..985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     963..985
                     /label=PA DSred2 sodB AU-rich sub4-F[������,739]
     primer_bind     963..985
                     /label=PA DSred2 sodB AU-rich sub3-F[������,737]
     primer_bind     963..985
                     /label=PA DSred2 sodB AU-rich sub2-F[������,735]
     primer_bind     963..985
                     /label=PA DSred2 sodB AU-rich sub1-F[������,733]
     primer_bind     963..984
                     /label=PA_DSred2_AU-richdelF[JMK,1]
     primer_bind     963..984
                     /label=DSred_sodB_AU-rich_F[JMK,13]
     primer_bind     963..984
                     /label=DSred_sodB_AU-rich_subF[JMK,30]
     primer_bind     963..980
                     /label=PA_DSred2_AU-richsubsF[JMK,3]
     primer_bind     963..979
                     /label=lacz_RBS_R[SMH-HFQ,240]
     primer_bind     963..978
                     /label=GFP_X_R[SMH-etc.,178]
     primer_bind     963..977
                     /label=pQE_R[SMH-seq,202]
     primer_bind     963..977
                     /label=GFP_RE_sodB_AU-rich_sub4_F[JMK,38]
     primer_bind     963..977
                     /label=GFP_RE_sodB_AU-rich_sub3_F[JMK,36]
     primer_bind     963..977
                     /label=GFP_RE_sodB_AU-rich_sub2_F[JMK,34]
     primer_bind     963..977
                     /label=GFP_RE_sodB_AU-rich_sub_F[JMK,22]
     primer_bind     963..977
                     /label=GFP_RE_sodB_AU-rich_F[JMK,20]
     primer_bind     complement(967..1002)
                     /label=T7_promoter_R[������,723]
     promoter        complement(986..1004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(987..1004)
                     /label=T7_promoter[������,699]
     primer_bind     complement(987..1004)
                     /label=T7_promoter[SMH-seq,179]
     primer_bind     complement(987..1004)
                     /label=T7[univeralprimer,100]
     primer_bind     complement(987..1004)
                     /label=T7 promoter[������,751]
     primer_bind     1003..1037
                     /label=T7_prmoter_F[������,722]
     primer_bind     1023..1039
                     /label=T7_iPCR_F[PVo,1077]
     primer_bind     1023..1039
                     /label=LacI_F_NotI[������,718]
     primer_bind     complement(1031..1046)
                     /label=pETupstream[univeralprimer,79]
     primer_bind     1258..1281
                     /label=lacI gene F[������,1045]
     primer_bind     1258..1281
                     /label=lacI gene F-RFP[������,1003]
     primer_bind     1258..1276
                     /label=T7promoter-R[������,1000]
     primer_bind     1259..1281
                     /label=lacI gene F-RFP[������,1039]
     primer_bind     1306..1326
                     /label=lacI_F_xho[SMH-HFQ,235]
     promoter        1313..1390
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(1313..1341)
                     /label=lacIQ_Muta_F[SMH-etc.,403]
     primer_bind     1324..1348
                     /label=lacIQ_Muta_R[SMH-etc.,410]
     primer_bind     complement(1344..1378)
                     /label=lacI_modified_RBS_R[SMH-etc.,398]
     primer_bind     complement(1347..1378)
                     /label=lacI_modified_RBS_R[SMH-etc.,401]
     CDS             1391..2473
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1392..1418
                     /label=lacI_modified_RBS_F[SMH-etc.,397]
     primer_bind     1392..1415
                     /label=lacI_bglII_F[SMH-HFQ,387]
     primer_bind     1676..1696
                     /label=lac_I_(inside)seq_F[SMH-CRISPR,383]
     primer_bind     1801..1821
                     /label=up_Arm_F[SMH-CRISPR,295]
     primer_bind     complement(2187..2208)
                     /label=up_Arm_R[SMH-CRISPR,296]
     primer_bind     complement(2416..2436)
                     /label=PBBR_SacI_XhoI_R[SMH-etc.,390]
     primer_bind     complement(2441..2459)
                     /label=J23110_R[SMH-HFQ,238]
     primer_bind     complement(2468..2490)
                     /label=lacI_R_aatII[SMH-etc.,406]
     primer_bind     complement(2468..2490)
                     /label=LacI_R_Kpn[SMH-HFQ,236]
     primer_bind     complement(2468..2488)
                     /label=lacI_sacI_R[SMH-etc.,393]
     primer_bind     complement(2472..2494)
                     /label=lacI_bamHI_R[SMH-HFQ,386]
     primer_bind     complement(2472..2490)
                     /label=LacI_R_BglII[������,719]
     primer_bind     complement(2498..2519)
                     /label=lacI gene R[������,1046]
     primer_bind     complement(2498..2519)
                     /label=lacI gene R-RFP[������,1040]
     primer_bind     complement(2498..2519)
                     /label=lacI gene R-RFP[������,1004]
     primer_bind     complement(2572..2591)
                     /label=RV4[univeralprimer,93]
     primer_bind     complement(2572..2589)
                     /label=pET_ori-replace_NheI_R[PVo,1104]
     CDS             3282..3473
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3467..3489)
                     /label=pGEX-3[univeralprimer,84]
     primer_bind     complement(3540..3550)
                     /label=pBL-invR[������,876]
     primer_bind     3627..3647
                     /label=Cre_R_notI_aatt[SMH-kinase,432]
     primer_bind     3669..3689
                     /label=t5up_afIII_F[JMK,467]
     primer_bind     complement(3672..3693)
                     /label=placIq_XhoI_F[SMH-etc.,392]
     primer_bind     complement(3672..3693)
                     /label=placIq_XhoI_F[LHM-etc,619]
     primer_bind     3682..3701
                     /label=lacZa_Qpcr_R[JMK,452]
     primer_bind     3684..3701
                     /label=Km_AflII_R[������,712]
     primer_bind     complement(3689..3711)
                     /label=LacI_upstream_aatII_NotI[SMH-etc.,404]
     primer_bind     3785..3806
                     /label=pAWP78_Inv_EcoRI/speI_R[SMH-metanotroph,287]
     primer_bind     3807..3831
                     /label=Ori_R_aatII[SMH-kinase,424]
     primer_bind     3812..3832
                     /label=pTac_Inv_R[SMH-etc.,301]
     primer_bind     complement(3824..3844)
                     /label=T3~PUC_AatII_R[SMH-etc.,288]
     primer_bind     complement(3832..3851)
                     /label=Rlow[SMH-seq,164]
     primer_bind     3846..3868
                     /label=pC-backbone-R[������,860]
     primer_bind     complement(3847..3863)
                     /label=CymR_BglII_F[PVo,1084]
     primer_bind     complement(3865..3895)
                     /label=CumateR gene F-RFP[������,1036]
     primer_bind     complement(3865..3887)
                     /label=CumateR gene F[������,1041]
     primer_bind     complement(3869..3887)
                     /label=CymR_R_EcoRI[LHM-Time-bomb,590]
     rep_origin      complement(3903..4491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3903..3925
                     /label=pBL-invF[������,877]
     primer_bind     3960..3979
                     /label=pAWP89_5_F[N39][������,730]
     primer_bind     3970..3990
                     /label=P15AORI_inside_F[JMK,10]
     primer_bind     complement(4052..4071)
                     /label=pAWP89_5_sequencing[������,898]
     primer_bind     4502..4525
                     /label=KanR_bglII/ncoI_F[SMH-HFQ,381]
     primer_bind     4502..4525
                     /label=KanR_bgIII/ncol_F[SMH-kinase,421]
     primer_bind     4505..4525
                     /label=amp-sacR[������,926]
     primer_bind     4506..4524
                     /label=pET_ori-replace_KpnI_F[PVo,1103]
     primer_bind     4530..4548
                     /label=Insert_DownHA_R[Phuong Vo,1049]
     primer_bind     complement(4574..4598)
                     /label=Ori_F_sacI_bgIII[SMH-kinase,439]
     primer_bind     complement(4644..4661)
                     /label=pSC101_NdeI_F[������,879]
     primer_bind     complement(4644..4661)
                     /label=pSC101_NdeI_F[SMH-HFQ,277]
     primer_bind     4661..4684
                     /label=Amp_R[SMH-HFQ,253]
     CDS             complement(4662..5522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4662..4684
                     /label=pET21a-AmpR-R[������,916]
     primer_bind     4662..4684
                     /label=ampR_R_aatII_SalI[SMH-CRISPR,389]
     primer_bind     4662..4684
                     /label=AmpR_R_aatII_SalI[LHM-etc,625]
     primer_bind     4732..4751
                     /label=Amp_Qpcr_R2[SMH-etc.,414]
     primer_bind     4794..4811
                     /label=BsaI_AmpR_100_R[LHM-loxP,568]
     primer_bind     complement(4812..4832)
                     /label=Amp_qPCR_F[SMH-seq,267]
     primer_bind     complement(4866..4888)
                     /label=NotI_AmpR_100_F[LHM-loxP,567]
     primer_bind     complement(5169..5188)
                     /label=NotI_AmpR_400_F[LHM-loxP,572]
     primer_bind     complement(5510..5537)
                     /label=ampR_F_SalI[SMH-CRISPR,388]
     promoter        complement(5523..5627)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5523..5555
                     /label=AmpR_R[LHM-etc,624]
     primer_bind     complement(5610..5627)
                     /label=pET21a-AmpR-F[������,915]
     primer_bind     complement(5611..5632)
                     /label=AmpR_sphI_ndeI_F(orderedas""AmpR"")[SMH-etc.,394]
     primer_bind     complement(5623..5647)
                     /label=amp-sacF[������,925]
     primer_bind     complement(5625..5644)
                     /label=Amp_F[SMH-HFQ,252]
     primer_bind     5636..5653
                     /label=pQE30_seq_F[SMH-seq,181]
     rep_origin      complement(5654..6109)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6075..6093)
                     /label=pC-backbone-F[������,859]
     primer_bind     complement(6100..6120)
                     /label=pBL-invR[������,876]
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagcttgtac agctcgtcca
      181 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      241 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
      301 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      361 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
      421 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
      481 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      541 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
      601 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      661 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
      721 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
      781 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
      841 gcaccacccc ggtgaacagc tcctcgccct tgctcacgga tccacggcgc cgctgacggc
      901 gtttttttcg gcccatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg
      961 ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag
     1021 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     1081 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     1141 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     1201 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     1261 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat
     1321 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
     1381 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
     1441 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
     1501 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
     1561 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
     1621 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
     1681 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
     1741 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
     1801 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
     1861 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
     1921 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
     1981 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
     2041 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
     2101 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
     2161 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
     2221 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
     2281 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     2341 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     2401 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2461 aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat
     2521 ctcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     2581 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     2641 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     2701 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     2761 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccccacggg
     2821 tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg
     2881 gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt
     2941 ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa
     3001 cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc
     3061 taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt
     3121 ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc
     3181 gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca
     3241 ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa
     3301 ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca
     3361 acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg
     3421 agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     3481 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     3541 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     3601 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     3661 ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     3721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4321 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     4981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5641 aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     5701 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     5761 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     5821 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     5881 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     5941 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6001 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6061 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
//
