LOCUS       In-Vivo\Anneal          8004 bp    DNA     circular     06-Dec-2020
FEATURES             Location/Qualifiers
     CDS             6424..7227
                     /label=KlURA3
                     /note=Detected CDS 267aa
     CDS             172..900
                     /label=yEGFP
                     /note=Detected CDS 242aa
     promoter        6258..6423
                     /label=URA3p
                     /note=promoter
     misc_feature    3402..3602
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(4018..4632)
                     /label=pUC
                     /note=pUC Origin
     misc_feature    7446..27
                     /label=TEF1P
                     /note=misc_feature
     CDS             1674..3401
                     /label=OzTIR1
                     /note=CDS
     CDS             complement(4792..5652)
                     /label=bla
                     /note=Ampicilin Resistance
     CDS             40..171
                     /label=AID*
                     /note=CDS
     promoter        1215..1664
                     /label=ACS2 promoter
                     /note=promoter
     terminator      907..1206
                     /label=PGK1 terminator
                     /note=terminator
     promoter        1215..1664
                     /label=ACS2p
                     /note=promoter
     promoter        complement(5653..5757)
                     /label=AmpR promoter
                     /note=promoter
     CDS             181..891
                     /label=yeGFP
                     /note=codons optimized for translation in S. cerevisiae and C. albicans
     primer_bind     complement(208..236)
                     /label=GFP-R
                     /note=GFP, reverse primer. Does NOT anneal to EGFP
     primer_bind     complement(3631..3647)
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     primer_bind     complement(3644..3666)
                     /label=M13/pUC Reverse
                     /note=In lacZ gene
     protein_bind    3655..3671
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(3679..3709)
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    3724..3745
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presence of cAMP.
     primer_bind     complement(3862..3879)
                     /label=L4440
                     /note=L4440 vector, forward primer
     rep_origin      complement(4033..4621)
                     /label=ori
                     /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
     primer_bind     complement(4113..4132)
                     /label=pBR322ori-F
                     /note=pBR322 origin, forward primer
     primer_bind     5415..5434
                     /label=Amp-R
                     /note=Ampicillin resistance gene, reverse primer
     primer_bind     5825..5843
                     /label=pBRforEco
                     /note=pBR322 vectors, upsteam of EcoRI site, forward primer
     primer_bind     complement(5881..5903)
                     /label=pGEX 3'
                     /note=pGEX vectors, reverse primer
     primer_bind     6003..6022
                     /label=pRS-marker
                     /note=pRS vectors, use to sequence yeast selectable marker
     primer_bind     6216..6238
                     /label=M13/pUC Forward
                     /note=In lacZ gene
     primer_bind     6230..6247
                     /label=M13 Forward
                     /note=In lacZ gene. Also called M13-F20 or M13 (-21) Forward
     primer_bind     6231..6247
                     /label=M13 fwd
                     /note=common sequencing primer, one of multiple similar variants
     protein_bind    7361..7394
                     /label=loxP
                     /note=Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
     CDS             4562..4570
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(3854..3862)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             1674..3398
                     /label=OzTIR1
                     /note=CDS
     CDS             4562..4570
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(3854..3862)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(3854..3862)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             4562..4570
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             4562..4570
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(3854..3862)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
BASE COUNT     2259 a      1625 c      1752 g      2368 t 
ORIGIN
        1 CAATCTAATC TAAGTTTTAA TTACAAACTC GAGAAAATGC CAAAAGACCC AGCTAAACCT 
       61 CCAGCCAAGG CCCAAGTCGT CGGCTGGCCA CCAGTTAGGT CTTACAGAAA GAACGTAATG 
      121 GTTAGTTGTC AAAAGTCCTC CGGTGGTCCA GAAGCTGCTG CCTTTGTTAA GATGGGATCC 
      181 TCTAAAGGTG AAGAATTATT CACTGGTGTT GTCCCAATTT TGGTTGAATT AGATGGTGAT 
      241 GTTAATGGTC ACAAATTTTC TGTCTCCGGT GAAGGTGAAG GTGATGCTAC TTACGGTAAA 
      301 TTGACCTTAA AATTTATTTG TACTACTGGT AAATTGCCAG TTCCATGGCC AACCTTAGTC 
      361 ACTACTTTCG GTTATGGTGT TCAATGTTTT GCGAGATACC CAGATCATAT GAAACAACAT 
      421 GACTTTTTCA AGTCTGCCAT GCCAGAAGGT TATGTTCAAG AAAGAACTAT TTTTTTCAAA 
      481 GATGACGGTA ACTACAAGAC CAGAGCTGAA GTCAAGTTTG AAGGTGATAC CTTAGTTAAT 
      541 AGAATCGAAT TAAAAGGTAT TGATTTTAAA GAAGATGGTA ACATTTTAGG TCACAAATTG 
      601 GAATACAACT ATAACTCTCA CAATGTTTAC ATCATGGCTG ACAAACAAAA GAATGGTATC 
      661 AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGTT CTGTTCAATT AGCTGACCAT 
      721 TATCAACAAA ATACTCCAAT TGGTGATGGT CCAGTCTTGT TACCAGACAA CCATTACTTA 
      781 TCCACTCAAT CTGCCTTATC CAAAGATCCA AACGAAAAGA GAGACCACAT GGTCTTGTTA 
      841 GAATTTGTTA CTGCTGCTGG TATTACCCAT GGTATGGATG AATTGTACAA AAGATCTTAA 
      901 ACTAGTATTG AATTGAATTG AAATCGATAG ATCAATTTTT TTCTTTTCTC TTTCCCCATC 
      961 CTTTACGCTA AAATAATAGT TTATTTTATT TTTTGAATAT TTTTTATTTA TATACGTATA 
     1021 TATAGACTAT TATTTATCTT TTAATGATTA TTAAGATTTT TATTAAAAAA AAATTCGCTC 
     1081 CTCTTTTAAT GCCTTTATGC AGTTTTTTTT TCCCATTCGA TATTTCTATG TTCGGGTTCA 
     1141 GCGTATTTTA AGTTTAATAA CTCGAAAATT CTGCGTTCGT TAAAGCTTTC GAGAAGGATA 
     1201 TTATTTGCGG CCGCGATTGC AAGAATTGAT CTCCCTCATC GATGTCATAA ATCAGTAAAT 
     1261 ATAAACATTA AATACATTAC ACGTAGCCAC CCGCTGTATA GCATAAAACT AACTTAAAAT 
     1321 TATTCATATG CGTTTCCCGG GGCCGAAGCG TTATTGCCGA TATTCGGTAG CCGATTCCGC 
     1381 TGGCTTTTTT TTTTTGAACG AGGCAAAAAA GTTTCGTGAC TGAACTTCTT CGTCACTGGT 
     1441 TTTGTTGATA ATTTTTATCG TTTTTTACTT CGAGATTGAA AACAGTGTTC TGAATTTTAG 
     1501 CAACGATATC GCACCGCACG TTTAGCCTGA TCAAACCTTA TATATACCTC TACCTTAAAA 
     1561 TATATATATA TATAGTATTC GAATTCCGAA TTGTGTGTCT CCGCGGTTAG TGATTGTTAT 
     1621 ACACAAACAG AATACAGGAA AGTAAATCAA TACAATAATA AATTGCTAGC AAAATGACCT 
     1681 ATTTCCCAGA AGAAGTCGTT GAACACATCT TCTCTTTCTT GCCAGCTCAA AGAGATAGAA 
     1741 ATACTGTTTC CTTGGTTTGT AAGGTTTGGT ATGAAATCGA AAGATTGAGT AGACGTGGTG 
     1801 TTTTCGTCGG TAATTGTTAC GCTGTTAGAG CTGGTAGAGT TGCTGCTAGA TTCCCAAATG 
     1861 TTAGAGCCTT GACTGTCAAA GGTAAACCAC ATTTTGCTGA CTTTAACTTG GTCCCACCAG 
     1921 ATTGGGGTGG TTATGCTGGT CCATGGATTG AAGCTGCCGC TAGAGGTTGT CACGGTTTGG 
     1981 AAGAATTGCG TATGAAAAGA ATGGTCGTTT CTGACGAATC TTTGGAATTA TTGGCCCGTT 
     2041 CTTTTCCAAG ATTCAGAGCT TTGGTTTTGA TCTCTTGTGA AGGTTTCAGT ACTGACGGTT 
     2101 TGGCTGCCGT TGCTTCCCAT TGTAAATTAT TGAGAGAATT GGACTTGCAA GAAAATGAAG 
     2161 TCGAAGATAG AGGTCCAAGA TGGTTATCTT GTTTTCCAGA CTCTTGTACT TCTTTGGTCT 
     2221 CTTTGAACTT CGCTTGTATT AAGGGTGAAG TCAACGCTGG TTCTTTAGAA AGATTGGTCT 
     2281 CCCGTTCTCC AAACTTACGT TCTTTGAGAT TGAATCGTTC TGTTTCCGTT GATACCTTGG 
     2341 CTAAAATCTT ATTGAGAACC CCAAACTTGG AAGACTTAGG TACTGGTAAC TTGACCGACG 
     2401 ATTTCCAAAC TGAATCCTAC TTTAAATTAA CTTCTGCTTT AGAAAAGTGT AAGATGTTGA 
     2461 GATCCTTATC TGGTTTTTGG GACGCCTCTC CAGTCTGTTT ATCTTTTATC TATCCATTGT 
     2521 GTGCTCAATT AACTGGTTTA AACTTATCTT ACGCTCCAAC CTTGGATGCT AGTGACTTAA 
     2581 CCAAGATGAT TAGTAGATGT GTTAAGTTAC AAAGATTATG GGTTTTAGAT TGTATTTCCG 
     2641 ATAAGGGTTT ACAAGTCGTT GCTAGTAGCT GTAAGGATTT GCAAGAATTA AGAGTCTTTC 
     2701 CAAGTGATTT TTACGTTGCT GGTTACAGCG CTGTTACTGA AGAAGGTTTG GTCGCTGTTT 
     2761 CTTTGGGTTG TCCAAAGTTG AACAGCTTAT TGTACTTTTG TCATCAAATG ACTAATGCTG 
     2821 CCTTAGTCAC TGTTGCTAAG AACTGTCCAA ACTTCACCCG TTTCAGATTG TGTATTTTGG 
     2881 AACCAGGTAA ACCAGACGTT GTCACCTCCC AACCATTGGA TGAAGGTTTC GGTGCTATCG 
     2941 TTAGAGAATG TAAGGGTTTG CAAAGATTAT CTATCTCTGG TTTGTTAACT GACAAAGTTT 
     3001 TCATGTACAT CGGTAAATAT GCCAAACAAT TAGAAATGTT GAGCATTGCT TTCGCTGGTG 
     3061 ACTCTGACAA GGGTATGATG CACGTTATGA ATGGTTGTAA GAATTTGAGA AAGTTGGAAA 
     3121 TTAGAGATTC TCCATTCGGT GACGCTGCCT TATTGGGTAA TTTTGCTAGA TACGAAACTA 
     3181 TGAGAAGTTT GTGGATGAGT TCCTGTAACG TTACCTTGAA AGGTTGTCAA GTCTTAGCCA 
     3241 GCAAGATGCC AATGTTGAAC GTCGAAGTCA TCAACGAAAG AGACGGTAGT AACGAAATGG 
     3301 AAGAAAATCA CGGTGACTTA CCAAAGGTAG AAAAGTTGTA CGTATACAGA ACTACTGCTG 
     3361 GTGCTAGAGA TGATGCTCCA AATTTCGTAA AGATTTTGTA AAGGACTATT TGCAAAGGGA 
     3421 AGGGATGCTA AGGTAGAGGG TGAACGTTAC AGAAAAGCAG GCTGGGAAGC ATATTTGAGA 
     3481 AGATGCGGCC AGCAAAACTA AAAAACTGTA TTATAAGTAA ATGCATGTAT ACTAAACTCA 
     3541 CAAATTAGAG CTTCAATTTA ATTATATCAG TTATTACCCG GGAATCTCGG TCGTAATGAT 
     3601 TTAAATGCAT GCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 
     3661 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 
     3721 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 
     3781 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 
     3841 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 
     3901 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3961 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4021 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4081 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4141 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4201 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4261 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4321 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4381 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4441 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 
     4501 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4561 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4621 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4681 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4741 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 
     4801 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 
     4861 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 
     4921 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 
     4981 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 
     5041 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 
     5101 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 
     5161 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 
     5221 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 
     5281 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 
     5341 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 
     5401 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 
     5461 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 
     5521 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 
     5581 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 
     5641 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
     5701 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 
     5761 ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA 
     5821 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA 
     5881 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG 
     5941 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA 
     6001 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG 
     6061 ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG 
     6121 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC 
     6181 TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC 
     6241 GGCCAGTGAA TTCATTTAAA TTTTTTTGAT TCGGTAATCT CCGAGCAGAA GGAAGAACGA 
     6301 AGGAAGGAGC ACAGACTTAG ATTGGTATAT ATACGCATAT GTGGTGTTGA AGAAACATGA 
     6361 AATTGCCCAG TATTCTTAAC CCAACTGCAC AGAACAAAAA CCTGCAGGAA ACGAAGATAA 
     6421 AACATGTCCA CAAAATCATA TACCAGTAGA GCTGAGACTC ATGCAAGTCC GGTTGCATCG 
     6481 AAACTTTTAC GTTTAATGGA TGAAAAGAAG ACCAATTTGT GTGCTTCTCT TGACGTTCGT 
     6541 TCGACTGATG AGCTATTGAA ACTTGTTGAA ACGTTGGGTC CATACATTTG CCTTTTGAAA 
     6601 ACACACGTTG ATATCTTGGA TGATTTCAGT TATGAGGGTA CTGTCGTTCC ATTGAAAGCA 
     6661 TTGGCAGAGA AATACAAGTT CTTGATATTT GAGGACAGAA AATTCGCCGA TATCGGTAAC 
     6721 ACAGTCAAAT TACAATATAC ATCGGGCGTT TACCGTATCG CAGAATGGTC TGATATCACC 
     6781 AACGCCCACG GGGTTACTGG TGCTGGTATT GTTGCTGGCT TGAAACAAGG TGCGCAAGAG 
     6841 GTCACCAAAG AACCAAGGGG ATTATTGATG CTTGCTGAAT TGTCTTCCAA GGGTTCTCTA 
     6901 GCACACGGTG AATATACTAA GGGTACCGTT GATATTGCAA AGAGTGATAA AGATTTCGTT 
     6961 ATTGGGTTCA TTGCTCAGAA CGATATGGGA GGAAGAGAAG AAGGGTTTGA TTGGCTAATC 
     7021 ATGACCCCAG GTGTAGGTTT AGACGACAAA GGCGATGCAT TGGGTCAGCA GTACAGAACC 
     7081 GTCGACGAAG TTGTAAGTGG TGGATCAGAT ATCATCATTG TTGGCAGAGG ACTTTTCGCC 
     7141 AAGGGTAGAG ATCCTAAGGT TGAAGGTGAA AGATACAGAA ATGCTGGATG GGAAGCGTAC 
     7201 CAAAAGAGAA TCAGCGCTCC CCATTAATTA TACAGGAAAC TTAATAGAAC AAATCACATA 
     7261 TTTAATCTAA TAGCCACCTG CATTGGCACG GTGCAACACT CACTTCAACT TCATCTTACA 
     7321 AAAGATCACG TGATCTGTTG TATTGGGATC TCTAGACCTA ATAACTTCGT ATAGCATACA 
     7381 TTATACGAAG TTATATTAAG GGTTGCTCGA CCAGAAAGCG ACCACCCAAC TTTGGCTGAT 
     7441 AATAGCGTAT AAACAATGCA TACTTTGTAC GTTCAAAATA CAATGCAGTA GATATATTTA 
     7501 TGCATATTAC ATATAATACA TATCACATAG GAAGCAACAG GCGCGTTGGA CTTTTAATTT 
     7561 TCGAGGACCG CGAATCCTTA CATCACACCC AATCCCCCAC AAGTGATCCC CCACACACCA 
     7621 TAGCTTCAAA ATGTTTCTAC TCCTTTTTTA CTCTTCCAGA TTTTCTCGGA CTCCGCGCAT 
     7681 CGCCGTACCA CTTCAAAACA CCCAAGCACA GCATACTAAA TTTCCCCTCT TTCTTCCTCT 
     7741 AGGGTGTCGT TAATTACCCG TACTAAAGGT TTGGAAAAGA AAAAAGAGAC CGCCTCGTTT 
     7801 CTTTTTCTTC GTCGAAAAAG GCAATAAAAA TTTTTATCAC GTTTCTTTTT CTTGAAAATT 
     7861 TTTTTTTTTG ATTTTTTTCT CTTTCGATGA CCTCCCATTG ATATTTAAGT TAATAAACGG 
     7921 TCTTCAATTT CTCAAGTTTC AGTTTCATTT TTCTTGTTCT ATTACAACTT TTTTTACTTC 
     7981 TTGCTCATTA GAAAGAAAGC ATAG 
//