LOCUS       pJAD5VY                 8184 bp    DNA     circular     03-May-2017
FEATURES             Location/Qualifiers
     CDS             6604..7407
                     /label=KlURA3
                     /note=Detected CDS 267aa
     CDS             352..1080
                     /label=yEGFP
                     /note=Detected CDS 242aa
     promoter        6438..6603
                     /label=URA3p
                     /note=promoter
     misc_feature    3582..3782
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(4198..4812)
                     /label=pUC
                     /note=pUC Origin
     misc_feature    7626..27
                     /label=TEF1P
                     /note=misc_feature
     CDS             1854..3581
                     /label=OzTIR1
                     /note=CDS
     CDS             complement(4972..5832)
                     /label=bla
                     /note=Ampicilin Resistance
     CDS             220..351
                     /label=AID*
                     /note=CDS
     CDS             37..219
                     /label=CUP1
                     /note=CDS
     promoter        1395..1844
                     /label=ACS2 promoter
                     /note=promoter
     terminator      1087..1386
                     /label=PGK1 terminator
                     /note=terminator
     promoter        1395..1844
                     /label=ACS2p
                     /note=promoter
     promoter        complement(5833..5937)
                     /label=AmpR promoter
                     /note=promoter
     CDS             361..1071
                     /label=yeGFP
                     /note=codons optimized for translation in S. cerevisiae and C. albicans
     primer_bind     complement(388..416)
                     /label=GFP-R
                     /note=GFP, reverse primer. Does NOT anneal to EGFP
     primer_bind     complement(3811..3827)
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     primer_bind     complement(3824..3846)
                     /label=M13/pUC Reverse
                     /note=In lacZ gene
     protein_bind    3835..3851
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(3859..3889)
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    3904..3925
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presence of cAMP.
     primer_bind     complement(4042..4059)
                     /label=L4440
                     /note=L4440 vector, forward primer
     rep_origin      complement(4213..4801)
                     /label=ori
                     /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
     primer_bind     complement(4293..4312)
                     /label=pBR322ori-F
                     /note=pBR322 origin, forward primer
     primer_bind     5595..5614
                     /label=Amp-R
                     /note=Ampicillin resistance gene, reverse primer
     primer_bind     6005..6023
                     /label=pBRforEco
                     /note=pBR322 vectors, upsteam of EcoRI site, forward primer
     primer_bind     complement(6061..6083)
                     /label=pGEX 3'
                     /note=pGEX vectors, reverse primer
     primer_bind     6183..6202
                     /label=pRS-marker
                     /note=pRS vectors, use to sequence yeast selectable marker
     primer_bind     6396..6418
                     /label=M13/pUC Forward
                     /note=In lacZ gene
     primer_bind     6410..6427
                     /label=M13 Forward
                     /note=In lacZ gene. Also called M13-F20 or M13 (-21) Forward
     primer_bind     6411..6427
                     /label=M13 fwd
                     /note=common sequencing primer, one of multiple similar variants
     protein_bind    7541..7574
                     /label=loxP
                     /note=Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
     CDS             4742..4750
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(4034..4042)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             1854..3578
                     /label=OzTIR1
                     /note=CDS
     CDS             4742..4750
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(4034..4042)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(4034..4042)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             4742..4750
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
BASE COUNT     2324 a      1663 c      1793 g      2404 t 
ORIGIN
        1 CAATCTAATC TAAGTTTTAA TTACAAACTC GAGAAAATGT TCAGCGAATT AATTAACTTC 
       61 CAAAATGAAG GTCATGAGTG CCAATGCCAA TGTGGTAGCT GCAAAAATAA TGAACAATGC 
      121 CAAAAATCAT GTAGCTGCCC AACGGGGTGT AACAGCGACG ACAAATGCCC CTGCGGTAAC 
      181 AAGTCTGAAG AAACCAAGAA GTCATGCTGC TCTGGGAAAC CAAAAGACCC AGCTAAACCT 
      241 CCAGCCAAGG CCCAAGTCGT CGGCTGGCCA CCAGTTAGGT CTTACAGAAA GAACGTAATG 
      301 GTTAGTTGTC AAAAGTCCTC CGGTGGTCCA GAAGCTGCTG CCTTTGTTAA GATGGGATCC 
      361 TCTAAAGGTG AAGAATTATT CACTGGTGTT GTCCCAATTT TGGTTGAATT AGATGGTGAT 
      421 GTTAATGGTC ACAAATTTTC TGTCTCCGGT GAAGGTGAAG GTGATGCTAC TTACGGTAAA 
      481 TTGACCTTAA AATTTATTTG TACTACTGGT AAATTGCCAG TTCCATGGCC AACCTTAGTC 
      541 ACTACTTTCG GTTATGGTGT TCAATGTTTT GCGAGATACC CAGATCATAT GAAACAACAT 
      601 GACTTTTTCA AGTCTGCCAT GCCAGAAGGT TATGTTCAAG AAAGAACTAT TTTTTTCAAA 
      661 GATGACGGTA ACTACAAGAC CAGAGCTGAA GTCAAGTTTG AAGGTGATAC CTTAGTTAAT 
      721 AGAATCGAAT TAAAAGGTAT TGATTTTAAA GAAGATGGTA ACATTTTAGG TCACAAATTG 
      781 GAATACAACT ATAACTCTCA CAATGTTTAC ATCATGGCTG ACAAACAAAA GAATGGTATC 
      841 AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGTT CTGTTCAATT AGCTGACCAT 
      901 TATCAACAAA ATACTCCAAT TGGTGATGGT CCAGTCTTGT TACCAGACAA CCATTACTTA 
      961 TCCACTCAAT CTGCCTTATC CAAAGATCCA AACGAAAAGA GAGACCACAT GGTCTTGTTA 
     1021 GAATTTGTTA CTGCTGCTGG TATTACCCAT GGTATGGATG AATTGTACAA AAGATCTTAA 
     1081 ACTAGTATTG AATTGAATTG AAATCGATAG ATCAATTTTT TTCTTTTCTC TTTCCCCATC 
     1141 CTTTACGCTA AAATAATAGT TTATTTTATT TTTTGAATAT TTTTTATTTA TATACGTATA 
     1201 TATAGACTAT TATTTATCTT TTAATGATTA TTAAGATTTT TATTAAAAAA AAATTCGCTC 
     1261 CTCTTTTAAT GCCTTTATGC AGTTTTTTTT TCCCATTCGA TATTTCTATG TTCGGGTTCA 
     1321 GCGTATTTTA AGTTTAATAA CTCGAAAATT CTGCGTTCGT TAAAGCTTTC GAGAAGGATA 
     1381 TTATTTGCGG CCGCGATTGC AAGAATTGAT CTCCCTCATC GATGTCATAA ATCAGTAAAT 
     1441 ATAAACATTA AATACATTAC ACGTAGCCAC CCGCTGTATA GCATAAAACT AACTTAAAAT 
     1501 TATTCATATG CGTTTCCCGG GGCCGAAGCG TTATTGCCGA TATTCGGTAG CCGATTCCGC 
     1561 TGGCTTTTTT TTTTTGAACG AGGCAAAAAA GTTTCGTGAC TGAACTTCTT CGTCACTGGT 
     1621 TTTGTTGATA ATTTTTATCG TTTTTTACTT CGAGATTGAA AACAGTGTTC TGAATTTTAG 
     1681 CAACGATATC GCACCGCACG TTTAGCCTGA TCAAACCTTA TATATACCTC TACCTTAAAA 
     1741 TATATATATA TATAGTATTC GAATTCCGAA TTGTGTGTCT CCGCGGTTAG TGATTGTTAT 
     1801 ACACAAACAG AATACAGGAA AGTAAATCAA TACAATAATA AATTGCTAGC AAAATGACCT 
     1861 ATTTCCCAGA AGAAGTCGTT GAACACATCT TCTCTTTCTT GCCAGCTCAA AGAGATAGAA 
     1921 ATACTGTTTC CTTGGTTTGT AAGGTTTGGT ATGAAATCGA AAGATTGAGT AGACGTGGTG 
     1981 TTTTCGTCGG TAATTGTTAC GCTGTTAGAG CTGGTAGAGT TGCTGCTAGA TTCCCAAATG 
     2041 TTAGAGCCTT GACTGTCAAA GGTAAACCAC ATTTTGCTGA CTTTAACTTG GTCCCACCAG 
     2101 ATTGGGGTGG TTATGCTGGT CCATGGATTG AAGCTGCCGC TAGAGGTTGT CACGGTTTGG 
     2161 AAGAATTGCG TATGAAAAGA ATGGTCGTTT CTGACGAATC TTTGGAATTA TTGGCCCGTT 
     2221 CTTTTCCAAG ATTCAGAGCT TTGGTTTTGA TCTCTTGTGA AGGTTTCAGT ACTGACGGTT 
     2281 TGGCTGCCGT TGCTTCCCAT TGTAAATTAT TGAGAGAATT GGACTTGCAA GAAAATGAAG 
     2341 TCGAAGATAG AGGTCCAAGA TGGTTATCTT GTTTTCCAGA CTCTTGTACT TCTTTGGTCT 
     2401 CTTTGAACTT CGCTTGTATT AAGGGTGAAG TCAACGCTGG TTCTTTAGAA AGATTGGTCT 
     2461 CCCGTTCTCC AAACTTACGT TCTTTGAGAT TGAATCGTTC TGTTTCCGTT GATACCTTGG 
     2521 CTAAAATCTT ATTGAGAACC CCAAACTTGG AAGACTTAGG TACTGGTAAC TTGACCGACG 
     2581 ATTTCCAAAC TGAATCCTAC TTTAAATTAA CTTCTGCTTT AGAAAAGTGT AAGATGTTGA 
     2641 GATCCTTATC TGGTTTTTGG GACGCCTCTC CAGTCTGTTT ATCTTTTATC TATCCATTGT 
     2701 GTGCTCAATT AACTGGTTTA AACTTATCTT ACGCTCCAAC CTTGGATGCT AGTGACTTAA 
     2761 CCAAGATGAT TAGTAGATGT GTTAAGTTAC AAAGATTATG GGTTTTAGAT TGTATTTCCG 
     2821 ATAAGGGTTT ACAAGTCGTT GCTAGTAGCT GTAAGGATTT GCAAGAATTA AGAGTCTTTC 
     2881 CAAGTGATTT TTACGTTGCT GGTTACAGCG CTGTTACTGA AGAAGGTTTG GTCGCTGTTT 
     2941 CTTTGGGTTG TCCAAAGTTG AACAGCTTAT TGTACTTTTG TCATCAAATG ACTAATGCTG 
     3001 CCTTAGTCAC TGTTGCTAAG AACTGTCCAA ACTTCACCCG TTTCAGATTG TGTATTTTGG 
     3061 AACCAGGTAA ACCAGACGTT GTCACCTCCC AACCATTGGA TGAAGGTTTC GGTGCTATCG 
     3121 TTAGAGAATG TAAGGGTTTG CAAAGATTAT CTATCTCTGG TTTGTTAACT GACAAAGTTT 
     3181 TCATGTACAT CGGTAAATAT GCCAAACAAT TAGAAATGTT GAGCATTGCT TTCGCTGGTG 
     3241 ACTCTGACAA GGGTATGATG CACGTTATGA ATGGTTGTAA GAATTTGAGA AAGTTGGAAA 
     3301 TTAGAGATTC TCCATTCGGT GACGCTGCCT TATTGGGTAA TTTTGCTAGA TACGAAACTA 
     3361 TGAGAAGTTT GTGGATGAGT TCCTGTAACG TTACCTTGAA AGGTTGTCAA GTCTTAGCCA 
     3421 GCAAGATGCC AATGTTGAAC GTCGAAGTCA TCAACGAAAG AGACGGTAGT AACGAAATGG 
     3481 AAGAAAATCA CGGTGACTTA CCAAAGGTAG AAAAGTTGTA CGTATACAGA ACTACTGCTG 
     3541 GTGCTAGAGA TGATGCTCCA AATTTCGTAA AGATTTTGTA AAGGACTATT TGCAAAGGGA 
     3601 AGGGATGCTA AGGTAGAGGG TGAACGTTAC AGAAAAGCAG GCTGGGAAGC ATATTTGAGA 
     3661 AGATGCGGCC AGCAAAACTA AAAAACTGTA TTATAAGTAA ATGCATGTAT ACTAAACTCA 
     3721 CAAATTAGAG CTTCAATTTA ATTATATCAG TTATTACCCG GGAATCTCGG TCGTAATGAT 
     3781 TTAAATGCAT GCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 
     3841 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 
     3901 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 
     3961 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 
     4021 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 
     4081 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4141 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4201 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4261 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4321 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4381 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4441 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4501 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4561 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4621 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 
     4681 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4741 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4801 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4861 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4921 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 
     4981 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 
     5041 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 
     5101 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 
     5161 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 
     5221 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 
     5281 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 
     5341 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 
     5401 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 
     5461 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 
     5521 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 
     5581 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 
     5641 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 
     5701 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 
     5761 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 
     5821 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
     5881 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 
     5941 ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA 
     6001 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA 
     6061 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG 
     6121 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA 
     6181 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG 
     6241 ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG 
     6301 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC 
     6361 TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC 
     6421 GGCCAGTGAA TTCATTTAAA TTTTTTTGAT TCGGTAATCT CCGAGCAGAA GGAAGAACGA 
     6481 AGGAAGGAGC ACAGACTTAG ATTGGTATAT ATACGCATAT GTGGTGTTGA AGAAACATGA 
     6541 AATTGCCCAG TATTCTTAAC CCAACTGCAC AGAACAAAAA CCTGCAGGAA ACGAAGATAA 
     6601 AACATGTCCA CAAAATCATA TACCAGTAGA GCTGAGACTC ATGCAAGTCC GGTTGCATCG 
     6661 AAACTTTTAC GTTTAATGGA TGAAAAGAAG ACCAATTTGT GTGCTTCTCT TGACGTTCGT 
     6721 TCGACTGATG AGCTATTGAA ACTTGTTGAA ACGTTGGGTC CATACATTTG CCTTTTGAAA 
     6781 ACACACGTTG ATATCTTGGA TGATTTCAGT TATGAGGGTA CTGTCGTTCC ATTGAAAGCA 
     6841 TTGGCAGAGA AATACAAGTT CTTGATATTT GAGGACAGAA AATTCGCCGA TATCGGTAAC 
     6901 ACAGTCAAAT TACAATATAC ATCGGGCGTT TACCGTATCG CAGAATGGTC TGATATCACC 
     6961 AACGCCCACG GGGTTACTGG TGCTGGTATT GTTGCTGGCT TGAAACAAGG TGCGCAAGAG 
     7021 GTCACCAAAG AACCAAGGGG ATTATTGATG CTTGCTGAAT TGTCTTCCAA GGGTTCTCTA 
     7081 GCACACGGTG AATATACTAA GGGTACCGTT GATATTGCAA AGAGTGATAA AGATTTCGTT 
     7141 ATTGGGTTCA TTGCTCAGAA CGATATGGGA GGAAGAGAAG AAGGGTTTGA TTGGCTAATC 
     7201 ATGACCCCAG GTGTAGGTTT AGACGACAAA GGCGATGCAT TGGGTCAGCA GTACAGAACC 
     7261 GTCGACGAAG TTGTAAGTGG TGGATCAGAT ATCATCATTG TTGGCAGAGG ACTTTTCGCC 
     7321 AAGGGTAGAG ATCCTAAGGT TGAAGGTGAA AGATACAGAA ATGCTGGATG GGAAGCGTAC 
     7381 CAAAAGAGAA TCAGCGCTCC CCATTAATTA TACAGGAAAC TTAATAGAAC AAATCACATA 
     7441 TTTAATCTAA TAGCCACCTG CATTGGCACG GTGCAACACT CACTTCAACT TCATCTTACA 
     7501 AAAGATCACG TGATCTGTTG TATTGGGATC TCTAGACCTA ATAACTTCGT ATAGCATACA 
     7561 TTATACGAAG TTATATTAAG GGTTGCTCGA CCAGAAAGCG ACCACCCAAC TTTGGCTGAT 
     7621 AATAGCGTAT AAACAATGCA TACTTTGTAC GTTCAAAATA CAATGCAGTA GATATATTTA 
     7681 TGCATATTAC ATATAATACA TATCACATAG GAAGCAACAG GCGCGTTGGA CTTTTAATTT 
     7741 TCGAGGACCG CGAATCCTTA CATCACACCC AATCCCCCAC AAGTGATCCC CCACACACCA 
     7801 TAGCTTCAAA ATGTTTCTAC TCCTTTTTTA CTCTTCCAGA TTTTCTCGGA CTCCGCGCAT 
     7861 CGCCGTACCA CTTCAAAACA CCCAAGCACA GCATACTAAA TTTCCCCTCT TTCTTCCTCT 
     7921 AGGGTGTCGT TAATTACCCG TACTAAAGGT TTGGAAAAGA AAAAAGAGAC CGCCTCGTTT 
     7981 CTTTTTCTTC GTCGAAAAAG GCAATAAAAA TTTTTATCAC GTTTCTTTTT CTTGAAAATT 
     8041 TTTTTTTTTG ATTTTTTTCT CTTTCGATGA CCTCCCATTG ATATTTAAGT TAATAAACGG 
     8101 TCTTCAATTT CTCAAGTTTC AGTTTCATTT TTCTTGTTCT ATTACAACTT TTTTTACTTC 
     8161 TTGCTCATTA GAAAGAAAGC ATAG 
//