LOCUS       In-Vivo\Anneal          8310 bp    DNA     circular     06-Dec-2020
FEATURES             Location/Qualifiers
     CDS             6249..7052
                     /label=KlURA3
                     /note=Detected CDS 267aa
     CDS             8307..725
                     /label=yEGFP
                     /note=Detected CDS 242aa
     promoter        6083..6248
                     /label=URA3p
                     /note=promoter
     misc_feature    3227..3427
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(3843..4457)
                     /label=pUC
                     /note=pUC Origin
     misc_feature    7271..7856
                     /label=TEF1P
                     /note=misc_feature
     CDS             1499..3226
                     /label=OzTIR1
                     /note=CDS
     CDS             complement(4617..5477)
                     /label=bla
                     /note=Ampicilin Resistance
     CDS             8175..8306
                     /label=AID*
                     /note=CDS
     CDS             7866..8174
                     /label=TRX1
                     /note=CDS
     promoter        1040..1489
                     /label=ACS2 promoter
                     /note=promoter
     terminator      732..1031
                     /label=PGK1 terminator
                     /note=terminator
     promoter        1040..1489
                     /label=ACS2p
                     /note=promoter
     promoter        complement(5478..5582)
                     /label=AmpR promoter
                     /note=promoter
     CDS             6..716
                     /label=yeGFP
                     /note=codons optimized for translation in S. cerevisiae and C. albicans
     primer_bind     complement(33..61)
                     /label=GFP-R
                     /note=GFP, reverse primer. Does NOT anneal to EGFP
     primer_bind     complement(3456..3472)
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     primer_bind     complement(3469..3491)
                     /label=M13/pUC Reverse
                     /note=In lacZ gene
     protein_bind    3480..3496
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(3504..3534)
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    3549..3570
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presence of cAMP.
     primer_bind     complement(3687..3704)
                     /label=L4440
                     /note=L4440 vector, forward primer
     rep_origin      complement(3858..4446)
                     /label=ori
                     /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
     primer_bind     complement(3938..3957)
                     /label=pBR322ori-F
                     /note=pBR322 origin, forward primer
     primer_bind     5240..5259
                     /label=Amp-R
                     /note=Ampicillin resistance gene, reverse primer
     primer_bind     5650..5668
                     /label=pBRforEco
                     /note=pBR322 vectors, upsteam of EcoRI site, forward primer
     primer_bind     complement(5706..5728)
                     /label=pGEX 3'
                     /note=pGEX vectors, reverse primer
     primer_bind     5828..5847
                     /label=pRS-marker
                     /note=pRS vectors, use to sequence yeast selectable marker
     primer_bind     6041..6063
                     /label=M13/pUC Forward
                     /note=In lacZ gene
     primer_bind     6055..6072
                     /label=M13 Forward
                     /note=In lacZ gene. Also called M13-F20 or M13 (-21) Forward
     primer_bind     6056..6072
                     /label=M13 fwd
                     /note=common sequencing primer, one of multiple similar variants
     protein_bind    7186..7219
                     /label=loxP
                     /note=Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
     CDS             4387..4395
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(3679..3687)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             1499..3223
                     /label=OzTIR1
                     /note=CDS
     CDS             4387..4395
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(3679..3687)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(3679..3687)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             4387..4395
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             4387..4395
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(3679..3687)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
BASE COUNT     2349 a      1688 c      1817 g      2456 t 
ORIGIN
        1 GATCCTCTAA AGGTGAAGAA TTATTCACTG GTGTTGTCCC AATTTTGGTT GAATTAGATG 
       61 GTGATGTTAA TGGTCACAAA TTTTCTGTCT CCGGTGAAGG TGAAGGTGAT GCTACTTACG 
      121 GTAAATTGAC CTTAAAATTT ATTTGTACTA CTGGTAAATT GCCAGTTCCA TGGCCAACCT 
      181 TAGTCACTAC TTTCGGTTAT GGTGTTCAAT GTTTTGCGAG ATACCCAGAT CATATGAAAC 
      241 AACATGACTT TTTCAAGTCT GCCATGCCAG AAGGTTATGT TCAAGAAAGA ACTATTTTTT 
      301 TCAAAGATGA CGGTAACTAC AAGACCAGAG CTGAAGTCAA GTTTGAAGGT GATACCTTAG 
      361 TTAATAGAAT CGAATTAAAA GGTATTGATT TTAAAGAAGA TGGTAACATT TTAGGTCACA 
      421 AATTGGAATA CAACTATAAC TCTCACAATG TTTACATCAT GGCTGACAAA CAAAAGAATG 
      481 GTATCAAAGT TAACTTCAAA ATTAGACACA ACATTGAAGA TGGTTCTGTT CAATTAGCTG 
      541 ACCATTATCA ACAAAATACT CCAATTGGTG ATGGTCCAGT CTTGTTACCA GACAACCATT 
      601 ACTTATCCAC TCAATCTGCC TTATCCAAAG ATCCAAACGA AAAGAGAGAC CACATGGTCT 
      661 TGTTAGAATT TGTTACTGCT GCTGGTATTA CCCATGGTAT GGATGAATTG TACAAAAGAT 
      721 CTTAAACTAG TATTGAATTG AATTGAAATC GATAGATCAA TTTTTTTCTT TTCTCTTTCC 
      781 CCATCCTTTA CGCTAAAATA ATAGTTTATT TTATTTTTTG AATATTTTTT ATTTATATAC 
      841 GTATATATAG ACTATTATTT ATCTTTTAAT GATTATTAAG ATTTTTATTA AAAAAAAATT 
      901 CGCTCCTCTT TTAATGCCTT TATGCAGTTT TTTTTTCCCA TTCGATATTT CTATGTTCGG 
      961 GTTCAGCGTA TTTTAAGTTT AATAACTCGA AAATTCTGCG TTCGTTAAAG CTTTCGAGAA 
     1021 GGATATTATT TGCGGCCGCG ATTGCAAGAA TTGATCTCCC TCATCGATGT CATAAATCAG 
     1081 TAAATATAAA CATTAAATAC ATTACACGTA GCCACCCGCT GTATAGCATA AAACTAACTT 
     1141 AAAATTATTC ATATGCGTTT CCCGGGGCCG AAGCGTTATT GCCGATATTC GGTAGCCGAT 
     1201 TCCGCTGGCT TTTTTTTTTT GAACGAGGCA AAAAAGTTTC GTGACTGAAC TTCTTCGTCA 
     1261 CTGGTTTTGT TGATAATTTT TATCGTTTTT TACTTCGAGA TTGAAAACAG TGTTCTGAAT 
     1321 TTTAGCAACG ATATCGCACC GCACGTTTAG CCTGATCAAA CCTTATATAT ACCTCTACCT 
     1381 TAAAATATAT ATATATATAG TATTCGAATT CCGAATTGTG TGTCTCCGCG GTTAGTGATT 
     1441 GTTATACACA AACAGAATAC AGGAAAGTAA ATCAATACAA TAATAAATTG CTAGCAAAAT 
     1501 GACCTATTTC CCAGAAGAAG TCGTTGAACA CATCTTCTCT TTCTTGCCAG CTCAAAGAGA 
     1561 TAGAAATACT GTTTCCTTGG TTTGTAAGGT TTGGTATGAA ATCGAAAGAT TGAGTAGACG 
     1621 TGGTGTTTTC GTCGGTAATT GTTACGCTGT TAGAGCTGGT AGAGTTGCTG CTAGATTCCC 
     1681 AAATGTTAGA GCCTTGACTG TCAAAGGTAA ACCACATTTT GCTGACTTTA ACTTGGTCCC 
     1741 ACCAGATTGG GGTGGTTATG CTGGTCCATG GATTGAAGCT GCCGCTAGAG GTTGTCACGG 
     1801 TTTGGAAGAA TTGCGTATGA AAAGAATGGT CGTTTCTGAC GAATCTTTGG AATTATTGGC 
     1861 CCGTTCTTTT CCAAGATTCA GAGCTTTGGT TTTGATCTCT TGTGAAGGTT TCAGTACTGA 
     1921 CGGTTTGGCT GCCGTTGCTT CCCATTGTAA ATTATTGAGA GAATTGGACT TGCAAGAAAA 
     1981 TGAAGTCGAA GATAGAGGTC CAAGATGGTT ATCTTGTTTT CCAGACTCTT GTACTTCTTT 
     2041 GGTCTCTTTG AACTTCGCTT GTATTAAGGG TGAAGTCAAC GCTGGTTCTT TAGAAAGATT 
     2101 GGTCTCCCGT TCTCCAAACT TACGTTCTTT GAGATTGAAT CGTTCTGTTT CCGTTGATAC 
     2161 CTTGGCTAAA ATCTTATTGA GAACCCCAAA CTTGGAAGAC TTAGGTACTG GTAACTTGAC 
     2221 CGACGATTTC CAAACTGAAT CCTACTTTAA ATTAACTTCT GCTTTAGAAA AGTGTAAGAT 
     2281 GTTGAGATCC TTATCTGGTT TTTGGGACGC CTCTCCAGTC TGTTTATCTT TTATCTATCC 
     2341 ATTGTGTGCT CAATTAACTG GTTTAAACTT ATCTTACGCT CCAACCTTGG ATGCTAGTGA 
     2401 CTTAACCAAG ATGATTAGTA GATGTGTTAA GTTACAAAGA TTATGGGTTT TAGATTGTAT 
     2461 TTCCGATAAG GGTTTACAAG TCGTTGCTAG TAGCTGTAAG GATTTGCAAG AATTAAGAGT 
     2521 CTTTCCAAGT GATTTTTACG TTGCTGGTTA CAGCGCTGTT ACTGAAGAAG GTTTGGTCGC 
     2581 TGTTTCTTTG GGTTGTCCAA AGTTGAACAG CTTATTGTAC TTTTGTCATC AAATGACTAA 
     2641 TGCTGCCTTA GTCACTGTTG CTAAGAACTG TCCAAACTTC ACCCGTTTCA GATTGTGTAT 
     2701 TTTGGAACCA GGTAAACCAG ACGTTGTCAC CTCCCAACCA TTGGATGAAG GTTTCGGTGC 
     2761 TATCGTTAGA GAATGTAAGG GTTTGCAAAG ATTATCTATC TCTGGTTTGT TAACTGACAA 
     2821 AGTTTTCATG TACATCGGTA AATATGCCAA ACAATTAGAA ATGTTGAGCA TTGCTTTCGC 
     2881 TGGTGACTCT GACAAGGGTA TGATGCACGT TATGAATGGT TGTAAGAATT TGAGAAAGTT 
     2941 GGAAATTAGA GATTCTCCAT TCGGTGACGC TGCCTTATTG GGTAATTTTG CTAGATACGA 
     3001 AACTATGAGA AGTTTGTGGA TGAGTTCCTG TAACGTTACC TTGAAAGGTT GTCAAGTCTT 
     3061 AGCCAGCAAG ATGCCAATGT TGAACGTCGA AGTCATCAAC GAAAGAGACG GTAGTAACGA 
     3121 AATGGAAGAA AATCACGGTG ACTTACCAAA GGTAGAAAAG TTGTACGTAT ACAGAACTAC 
     3181 TGCTGGTGCT AGAGATGATG CTCCAAATTT CGTAAAGATT TTGTAAAGGA CTATTTGCAA 
     3241 AGGGAAGGGA TGCTAAGGTA GAGGGTGAAC GTTACAGAAA AGCAGGCTGG GAAGCATATT 
     3301 TGAGAAGATG CGGCCAGCAA AACTAAAAAA CTGTATTATA AGTAAATGCA TGTATACTAA 
     3361 ACTCACAAAT TAGAGCTTCA ATTTAATTAT ATCAGTTATT ACCCGGGAAT CTCGGTCGTA 
     3421 ATGATTTAAA TGCATGCAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT 
     3481 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG 
     3541 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG 
     3601 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT 
     3661 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 
     3721 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 
     3781 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 
     3841 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 
     3901 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 
     3961 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 
     4021 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 
     4081 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 
     4141 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 
     4201 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 
     4261 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 
     4321 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 
     4381 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 
     4441 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 
     4501 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 
     4561 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 
     4621 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 
     4681 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 
     4741 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 
     4801 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 
     4861 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 
     4921 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 
     4981 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 
     5041 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 
     5101 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 
     5161 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 
     5221 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 
     5281 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 
     5341 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 
     5401 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 
     5461 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     5521 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     5581 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA 
     5641 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 
     5701 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 
     5761 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 
     5821 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 
     5881 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC 
     5941 TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA 
     6001 TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA 
     6061 ACGACGGCCA GTGAATTCAT TTAAATTTTT TTGATTCGGT AATCTCCGAG CAGAAGGAAG 
     6121 AACGAAGGAA GGAGCACAGA CTTAGATTGG TATATATACG CATATGTGGT GTTGAAGAAA 
     6181 CATGAAATTG CCCAGTATTC TTAACCCAAC TGCACAGAAC AAAAACCTGC AGGAAACGAA 
     6241 GATAAAACAT GTCCACAAAA TCATATACCA GTAGAGCTGA GACTCATGCA AGTCCGGTTG 
     6301 CATCGAAACT TTTACGTTTA ATGGATGAAA AGAAGACCAA TTTGTGTGCT TCTCTTGACG 
     6361 TTCGTTCGAC TGATGAGCTA TTGAAACTTG TTGAAACGTT GGGTCCATAC ATTTGCCTTT 
     6421 TGAAAACACA CGTTGATATC TTGGATGATT TCAGTTATGA GGGTACTGTC GTTCCATTGA 
     6481 AAGCATTGGC AGAGAAATAC AAGTTCTTGA TATTTGAGGA CAGAAAATTC GCCGATATCG 
     6541 GTAACACAGT CAAATTACAA TATACATCGG GCGTTTACCG TATCGCAGAA TGGTCTGATA 
     6601 TCACCAACGC CCACGGGGTT ACTGGTGCTG GTATTGTTGC TGGCTTGAAA CAAGGTGCGC 
     6661 AAGAGGTCAC CAAAGAACCA AGGGGATTAT TGATGCTTGC TGAATTGTCT TCCAAGGGTT 
     6721 CTCTAGCACA CGGTGAATAT ACTAAGGGTA CCGTTGATAT TGCAAAGAGT GATAAAGATT 
     6781 TCGTTATTGG GTTCATTGCT CAGAACGATA TGGGAGGAAG AGAAGAAGGG TTTGATTGGC 
     6841 TAATCATGAC CCCAGGTGTA GGTTTAGACG ACAAAGGCGA TGCATTGGGT CAGCAGTACA 
     6901 GAACCGTCGA CGAAGTTGTA AGTGGTGGAT CAGATATCAT CATTGTTGGC AGAGGACTTT 
     6961 TCGCCAAGGG TAGAGATCCT AAGGTTGAAG GTGAAAGATA CAGAAATGCT GGATGGGAAG 
     7021 CGTACCAAAA GAGAATCAGC GCTCCCCATT AATTATACAG GAAACTTAAT AGAACAAATC 
     7081 ACATATTTAA TCTAATAGCC ACCTGCATTG GCACGGTGCA ACACTCACTT CAACTTCATC 
     7141 TTACAAAAGA TCACGTGATC TGTTGTATTG GGATCTCTAG ACCTAATAAC TTCGTATAGC 
     7201 ATACATTATA CGAAGTTATA TTAAGGGTTG CTCGACCAGA AAGCGACCAC CCAACTTTGG 
     7261 CTGATAATAG CGTATAAACA ATGCATACTT TGTACGTTCA AAATACAATG CAGTAGATAT 
     7321 ATTTATGCAT ATTACATATA ATACATATCA CATAGGAAGC AACAGGCGCG TTGGACTTTT 
     7381 AATTTTCGAG GACCGCGAAT CCTTACATCA CACCCAATCC CCCACAAGTG ATCCCCCACA 
     7441 CACCATAGCT TCAAAATGTT TCTACTCCTT TTTTACTCTT CCAGATTTTC TCGGACTCCG 
     7501 CGCATCGCCG TACCACTTCA AAACACCCAA GCACAGCATA CTAAATTTCC CCTCTTTCTT 
     7561 CCTCTAGGGT GTCGTTAATT ACCCGTACTA AAGGTTTGGA AAAGAAAAAA GAGACCGCCT 
     7621 CGTTTCTTTT TCTTCGTCGA AAAAGGCAAT AAAAATTTTT ATCACGTTTC TTTTTCTTGA 
     7681 AAATTTTTTT TTTTGATTTT TTTCTCTTTC GATGACCTCC CATTGATATT TAAGTTAATA 
     7741 AACGGTCTTC AATTTCTCAA GTTTCAGTTT CATTTTTCTT GTTCTATTAC AACTTTTTTT 
     7801 ACTTCTTGCT CATTAGAAAG AAAGCATAGC AATCTAATCT AAGTTTTAAT TACAAACTCG 
     7861 AGAAAATGGT TACTCAATTC AAAACTGCCA GCGAATTCGA CTCTGCAATT GCTCAAGACA 
     7921 AGCTAGTTGT CGTAGATTTC TACGCCACTT GGTGCGGTCC ATGTAAAATG ATTGCTCCAA 
     7981 TGATTGAAAA ATTCTCTGAA CAATACCCAC AAGCTGATTT CTATAAATTG GATGTCGATG 
     8041 AATTGGGTGA TGTTGCACAA AAGAATGAAG TTTCCGCTAT GCCAACTTTG CTTCTATTCA 
     8101 AGAACGGTAA GGAAGTTGCA AAGGTTGTTG GTGCCAACCC AGCGGCTATT AAGCAAGCCA 
     8161 TTGCTGCTAA TGCTCCAAAA GACCCAGCTA AACCTCCAGC CAAGGCCCAA GTCGTCGGCT 
     8221 GGCCACCAGT TAGGTCTTAC AGAAAGAACG TAATGGTTAG TTGTCAAAAG TCCTCCGGTG 
     8281 GTCCAGAAGC TGCTGCCTTT GTTAAGATGG  
//