LOCUS       In-Vivo\Anneal          8463 bp    DNA     circular     06-Dec-2020
FEATURES             Location/Qualifiers
     CDS             6883..7686
                     /label=KlURA3
                     /note=Detected CDS 267aa
     CDS             631..1359
                     /label=yEGFP
                     /note=Detected CDS 242aa
     promoter        6717..6882
                     /label=URA3p
                     /note=promoter
     misc_feature    3861..4061
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(4477..5091)
                     /label=pUC
                     /note=pUC Origin
     misc_feature    7905..27
                     /label=TEF1P
                     /note=misc_feature
     CDS             2133..3860
                     /label=OzTIR1
                     /note=CDS
     CDS             complement(5251..6111)
                     /label=bla
                     /note=Ampicilin Resistance
     CDS             499..630
                     /label=AID*
                     /note=CDS
     CDS             37..498
                     /label=SOD1
                     /note=CDS
     promoter        1674..2123
                     /label=ACS2 promoter
                     /note=promoter
     terminator      1366..1665
                     /label=PGK1 terminator
                     /note=terminator
     promoter        1674..2123
                     /label=ACS2p
                     /note=promoter
     promoter        complement(6112..6216)
                     /label=AmpR promoter
                     /note=promoter
     CDS             640..1350
                     /label=yeGFP
                     /note=codons optimized for translation in S. cerevisiae and C. albicans
     primer_bind     complement(667..695)
                     /label=GFP-R
                     /note=GFP, reverse primer. Does NOT anneal to EGFP
     primer_bind     complement(4090..4106)
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     primer_bind     complement(4103..4125)
                     /label=M13/pUC Reverse
                     /note=In lacZ gene
     protein_bind    4114..4130
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(4138..4168)
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    4183..4204
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presence of cAMP.
     primer_bind     complement(4321..4338)
                     /label=L4440
                     /note=L4440 vector, forward primer
     rep_origin      complement(4492..5080)
                     /label=ori
                     /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
     primer_bind     complement(4572..4591)
                     /label=pBR322ori-F
                     /note=pBR322 origin, forward primer
     primer_bind     5874..5893
                     /label=Amp-R
                     /note=Ampicillin resistance gene, reverse primer
     primer_bind     6284..6302
                     /label=pBRforEco
                     /note=pBR322 vectors, upsteam of EcoRI site, forward primer
     primer_bind     complement(6340..6362)
                     /label=pGEX 3'
                     /note=pGEX vectors, reverse primer
     primer_bind     6462..6481
                     /label=pRS-marker
                     /note=pRS vectors, use to sequence yeast selectable marker
     primer_bind     6675..6697
                     /label=M13/pUC Forward
                     /note=In lacZ gene
     primer_bind     6689..6706
                     /label=M13 Forward
                     /note=In lacZ gene. Also called M13-F20 or M13 (-21) Forward
     primer_bind     6690..6706
                     /label=M13 fwd
                     /note=common sequencing primer, one of multiple similar variants
     protein_bind    7820..7853
                     /label=loxP
                     /note=Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
     CDS             5021..5029
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(4313..4321)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             2133..3857
                     /label=OzTIR1
                     /note=CDS
     CDS             5021..5029
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(4313..4321)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(4313..4321)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             5021..5029
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             5021..5029
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(4313..4321)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
BASE COUNT     2372 a      1735 c      1874 g      2482 t 
ORIGIN
        1 CAATCTAATC TAAGTTTTAA TTACAAACTC GAGAAAATGG TTCAAGCAGT CGCAGTGTTA 
       61 AAGGGTGATG CCGGTGTCTC TGGTGTTGTC AAGTTCGAAC AGGCTTCCGA ATCCGAGCCA 
      121 ACCACTGTCT CTTACGAGAT CGCTGGTAAC AGTCCTAACG CAGAACGTGG GTTCCACATT 
      181 CATGAGTTTG GAGATGCCAC CAATGGTTGT GTCTCTGCTG GTCCTCACTT CAATCCTTTC 
      241 AAGAAGACAC ATGGTGCTCC AACTGACGAA GTCAGACATG TCGGTGACAT GGGTAACGTA 
      301 AAGACGGACG AAAATGGTGT GGCCAAGGGC TCCTTCAAGG ACTCTTTGAT CAAGCTTATC 
      361 GGTCCTACCT CCGTTGTAGG CAGAAGCGTC GTTATCCACG CCGGCCAAGA TGACTTAGGT 
      421 AAGGGTGACA CTGAAGAATC TTTGAAGACT GGTAATGCCG GTCCAAGACC AGCCTGTGGT 
      481 GTCATTGGTC TAACCAACCC AAAAGACCCA GCTAAACCTC CAGCCAAGGC CCAAGTCGTC 
      541 GGCTGGCCAC CAGTTAGGTC TTACAGAAAG AACGTAATGG TTAGTTGTCA AAAGTCCTCC 
      601 GGTGGTCCAG AAGCTGCTGC CTTTGTTAAG ATGGGATCCT CTAAAGGTGA AGAATTATTC 
      661 ACTGGTGTTG TCCCAATTTT GGTTGAATTA GATGGTGATG TTAATGGTCA CAAATTTTCT 
      721 GTCTCCGGTG AAGGTGAAGG TGATGCTACT TACGGTAAAT TGACCTTAAA ATTTATTTGT 
      781 ACTACTGGTA AATTGCCAGT TCCATGGCCA ACCTTAGTCA CTACTTTCGG TTATGGTGTT 
      841 CAATGTTTTG CGAGATACCC AGATCATATG AAACAACATG ACTTTTTCAA GTCTGCCATG 
      901 CCAGAAGGTT ATGTTCAAGA AAGAACTATT TTTTTCAAAG ATGACGGTAA CTACAAGACC 
      961 AGAGCTGAAG TCAAGTTTGA AGGTGATACC TTAGTTAATA GAATCGAATT AAAAGGTATT 
     1021 GATTTTAAAG AAGATGGTAA CATTTTAGGT CACAAATTGG AATACAACTA TAACTCTCAC 
     1081 AATGTTTACA TCATGGCTGA CAAACAAAAG AATGGTATCA AAGTTAACTT CAAAATTAGA 
     1141 CACAACATTG AAGATGGTTC TGTTCAATTA GCTGACCATT ATCAACAAAA TACTCCAATT 
     1201 GGTGATGGTC CAGTCTTGTT ACCAGACAAC CATTACTTAT CCACTCAATC TGCCTTATCC 
     1261 AAAGATCCAA ACGAAAAGAG AGACCACATG GTCTTGTTAG AATTTGTTAC TGCTGCTGGT 
     1321 ATTACCCATG GTATGGATGA ATTGTACAAA AGATCTTAAA CTAGTATTGA ATTGAATTGA 
     1381 AATCGATAGA TCAATTTTTT TCTTTTCTCT TTCCCCATCC TTTACGCTAA AATAATAGTT 
     1441 TATTTTATTT TTTGAATATT TTTTATTTAT ATACGTATAT ATAGACTATT ATTTATCTTT 
     1501 TAATGATTAT TAAGATTTTT ATTAAAAAAA AATTCGCTCC TCTTTTAATG CCTTTATGCA 
     1561 GTTTTTTTTT CCCATTCGAT ATTTCTATGT TCGGGTTCAG CGTATTTTAA GTTTAATAAC 
     1621 TCGAAAATTC TGCGTTCGTT AAAGCTTTCG AGAAGGATAT TATTTGCGGC CGCGATTGCA 
     1681 AGAATTGATC TCCCTCATCG ATGTCATAAA TCAGTAAATA TAAACATTAA ATACATTACA 
     1741 CGTAGCCACC CGCTGTATAG CATAAAACTA ACTTAAAATT ATTCATATGC GTTTCCCGGG 
     1801 GCCGAAGCGT TATTGCCGAT ATTCGGTAGC CGATTCCGCT GGCTTTTTTT TTTTGAACGA 
     1861 GGCAAAAAAG TTTCGTGACT GAACTTCTTC GTCACTGGTT TTGTTGATAA TTTTTATCGT 
     1921 TTTTTACTTC GAGATTGAAA ACAGTGTTCT GAATTTTAGC AACGATATCG CACCGCACGT 
     1981 TTAGCCTGAT CAAACCTTAT ATATACCTCT ACCTTAAAAT ATATATATAT ATAGTATTCG 
     2041 AATTCCGAAT TGTGTGTCTC CGCGGTTAGT GATTGTTATA CACAAACAGA ATACAGGAAA 
     2101 GTAAATCAAT ACAATAATAA ATTGCTAGCA AAATGACCTA TTTCCCAGAA GAAGTCGTTG 
     2161 AACACATCTT CTCTTTCTTG CCAGCTCAAA GAGATAGAAA TACTGTTTCC TTGGTTTGTA 
     2221 AGGTTTGGTA TGAAATCGAA AGATTGAGTA GACGTGGTGT TTTCGTCGGT AATTGTTACG 
     2281 CTGTTAGAGC TGGTAGAGTT GCTGCTAGAT TCCCAAATGT TAGAGCCTTG ACTGTCAAAG 
     2341 GTAAACCACA TTTTGCTGAC TTTAACTTGG TCCCACCAGA TTGGGGTGGT TATGCTGGTC 
     2401 CATGGATTGA AGCTGCCGCT AGAGGTTGTC ACGGTTTGGA AGAATTGCGT ATGAAAAGAA 
     2461 TGGTCGTTTC TGACGAATCT TTGGAATTAT TGGCCCGTTC TTTTCCAAGA TTCAGAGCTT 
     2521 TGGTTTTGAT CTCTTGTGAA GGTTTCAGTA CTGACGGTTT GGCTGCCGTT GCTTCCCATT 
     2581 GTAAATTATT GAGAGAATTG GACTTGCAAG AAAATGAAGT CGAAGATAGA GGTCCAAGAT 
     2641 GGTTATCTTG TTTTCCAGAC TCTTGTACTT CTTTGGTCTC TTTGAACTTC GCTTGTATTA 
     2701 AGGGTGAAGT CAACGCTGGT TCTTTAGAAA GATTGGTCTC CCGTTCTCCA AACTTACGTT 
     2761 CTTTGAGATT GAATCGTTCT GTTTCCGTTG ATACCTTGGC TAAAATCTTA TTGAGAACCC 
     2821 CAAACTTGGA AGACTTAGGT ACTGGTAACT TGACCGACGA TTTCCAAACT GAATCCTACT 
     2881 TTAAATTAAC TTCTGCTTTA GAAAAGTGTA AGATGTTGAG ATCCTTATCT GGTTTTTGGG 
     2941 ACGCCTCTCC AGTCTGTTTA TCTTTTATCT ATCCATTGTG TGCTCAATTA ACTGGTTTAA 
     3001 ACTTATCTTA CGCTCCAACC TTGGATGCTA GTGACTTAAC CAAGATGATT AGTAGATGTG 
     3061 TTAAGTTACA AAGATTATGG GTTTTAGATT GTATTTCCGA TAAGGGTTTA CAAGTCGTTG 
     3121 CTAGTAGCTG TAAGGATTTG CAAGAATTAA GAGTCTTTCC AAGTGATTTT TACGTTGCTG 
     3181 GTTACAGCGC TGTTACTGAA GAAGGTTTGG TCGCTGTTTC TTTGGGTTGT CCAAAGTTGA 
     3241 ACAGCTTATT GTACTTTTGT CATCAAATGA CTAATGCTGC CTTAGTCACT GTTGCTAAGA 
     3301 ACTGTCCAAA CTTCACCCGT TTCAGATTGT GTATTTTGGA ACCAGGTAAA CCAGACGTTG 
     3361 TCACCTCCCA ACCATTGGAT GAAGGTTTCG GTGCTATCGT TAGAGAATGT AAGGGTTTGC 
     3421 AAAGATTATC TATCTCTGGT TTGTTAACTG ACAAAGTTTT CATGTACATC GGTAAATATG 
     3481 CCAAACAATT AGAAATGTTG AGCATTGCTT TCGCTGGTGA CTCTGACAAG GGTATGATGC 
     3541 ACGTTATGAA TGGTTGTAAG AATTTGAGAA AGTTGGAAAT TAGAGATTCT CCATTCGGTG 
     3601 ACGCTGCCTT ATTGGGTAAT TTTGCTAGAT ACGAAACTAT GAGAAGTTTG TGGATGAGTT 
     3661 CCTGTAACGT TACCTTGAAA GGTTGTCAAG TCTTAGCCAG CAAGATGCCA ATGTTGAACG 
     3721 TCGAAGTCAT CAACGAAAGA GACGGTAGTA ACGAAATGGA AGAAAATCAC GGTGACTTAC 
     3781 CAAAGGTAGA AAAGTTGTAC GTATACAGAA CTACTGCTGG TGCTAGAGAT GATGCTCCAA 
     3841 ATTTCGTAAA GATTTTGTAA AGGACTATTT GCAAAGGGAA GGGATGCTAA GGTAGAGGGT 
     3901 GAACGTTACA GAAAAGCAGG CTGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA 
     3961 AAAACTGTAT TATAAGTAAA TGCATGTATA CTAAACTCAC AAATTAGAGC TTCAATTTAA 
     4021 TTATATCAGT TATTACCCGG GAATCTCGGT CGTAATGATT TAAATGCATG CAAGCTTGGC 
     4081 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 
     4141 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 
     4201 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 
     4261 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 
     4321 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 
     4381 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4441 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4501 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4561 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4621 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4681 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4741 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4801 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4861 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4921 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4981 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5041 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5101 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5161 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5221 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 
     5281 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 
     5341 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 
     5401 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 
     5461 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 
     5521 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 
     5581 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 
     5641 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 
     5701 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 
     5761 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 
     5821 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 
     5881 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 
     5941 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 
     6001 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 
     6061 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 
     6121 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 
     6181 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 
     6241 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG 
     6301 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC 
     6361 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC 
     6421 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT 
     6481 ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG 
     6541 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC 
     6601 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT 
     6661 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCATTTAAAT 
     6721 TTTTTTGATT CGGTAATCTC CGAGCAGAAG GAAGAACGAA GGAAGGAGCA CAGACTTAGA 
     6781 TTGGTATATA TACGCATATG TGGTGTTGAA GAAACATGAA ATTGCCCAGT ATTCTTAACC 
     6841 CAACTGCACA GAACAAAAAC CTGCAGGAAA CGAAGATAAA ACATGTCCAC AAAATCATAT 
     6901 ACCAGTAGAG CTGAGACTCA TGCAAGTCCG GTTGCATCGA AACTTTTACG TTTAATGGAT 
     6961 GAAAAGAAGA CCAATTTGTG TGCTTCTCTT GACGTTCGTT CGACTGATGA GCTATTGAAA 
     7021 CTTGTTGAAA CGTTGGGTCC ATACATTTGC CTTTTGAAAA CACACGTTGA TATCTTGGAT 
     7081 GATTTCAGTT ATGAGGGTAC TGTCGTTCCA TTGAAAGCAT TGGCAGAGAA ATACAAGTTC 
     7141 TTGATATTTG AGGACAGAAA ATTCGCCGAT ATCGGTAACA CAGTCAAATT ACAATATACA 
     7201 TCGGGCGTTT ACCGTATCGC AGAATGGTCT GATATCACCA ACGCCCACGG GGTTACTGGT 
     7261 GCTGGTATTG TTGCTGGCTT GAAACAAGGT GCGCAAGAGG TCACCAAAGA ACCAAGGGGA 
     7321 TTATTGATGC TTGCTGAATT GTCTTCCAAG GGTTCTCTAG CACACGGTGA ATATACTAAG 
     7381 GGTACCGTTG ATATTGCAAA GAGTGATAAA GATTTCGTTA TTGGGTTCAT TGCTCAGAAC 
     7441 GATATGGGAG GAAGAGAAGA AGGGTTTGAT TGGCTAATCA TGACCCCAGG TGTAGGTTTA 
     7501 GACGACAAAG GCGATGCATT GGGTCAGCAG TACAGAACCG TCGACGAAGT TGTAAGTGGT 
     7561 GGATCAGATA TCATCATTGT TGGCAGAGGA CTTTTCGCCA AGGGTAGAGA TCCTAAGGTT 
     7621 GAAGGTGAAA GATACAGAAA TGCTGGATGG GAAGCGTACC AAAAGAGAAT CAGCGCTCCC 
     7681 CATTAATTAT ACAGGAAACT TAATAGAACA AATCACATAT TTAATCTAAT AGCCACCTGC 
     7741 ATTGGCACGG TGCAACACTC ACTTCAACTT CATCTTACAA AAGATCACGT GATCTGTTGT 
     7801 ATTGGGATCT CTAGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATTAAGG 
     7861 GTTGCTCGAC CAGAAAGCGA CCACCCAACT TTGGCTGATA ATAGCGTATA AACAATGCAT 
     7921 ACTTTGTACG TTCAAAATAC AATGCAGTAG ATATATTTAT GCATATTACA TATAATACAT 
     7981 ATCACATAGG AAGCAACAGG CGCGTTGGAC TTTTAATTTT CGAGGACCGC GAATCCTTAC 
     8041 ATCACACCCA ATCCCCCACA AGTGATCCCC CACACACCAT AGCTTCAAAA TGTTTCTACT 
     8101 CCTTTTTTAC TCTTCCAGAT TTTCTCGGAC TCCGCGCATC GCCGTACCAC TTCAAAACAC 
     8161 CCAAGCACAG CATACTAAAT TTCCCCTCTT TCTTCCTCTA GGGTGTCGTT AATTACCCGT 
     8221 ACTAAAGGTT TGGAAAAGAA AAAAGAGACC GCCTCGTTTC TTTTTCTTCG TCGAAAAAGG 
     8281 CAATAAAAAT TTTTATCACG TTTCTTTTTC TTGAAAATTT TTTTTTTTGA TTTTTTTCTC 
     8341 TTTCGATGAC CTCCCATTGA TATTTAAGTT AATAAACGGT CTTCAATTTC TCAAGTTTCA 
     8401 GTTTCATTTT TCTTGTTCTA TTACAACTTT TTTTACTTCT TGCTCATTAG AAAGAAAGCA 
     8461 TAG 
//