LOCUS pJAD8Z 8495 bp DNA circular 07-Feb-2018 FEATURES Location/Qualifiers CDS 5425..6228 /label=KlURA3 /note=Detected CDS 267aa CDS 7033..7761 /label=yEGFP /note=Detected CDS 242aa promoter 5259..5424 /label=URA3p /note=promoter misc_feature 2403..2603 /label=URA3(705, 905) /note=misc_feature rep_origin complement(3019..3633) /label=pUC /note=pUC Origin misc_feature 6447..7032 /label=TEF1P /note=misc_feature CDS 669..2396 /label=OzTIR1 /note=CDS CDS complement(3793..4653) /label=bla /note=Ampicilin Resistance terminator 7768..8067 /label=PGK1 terminator /note=terminator promoter complement(4654..4758) /label=AmpR promoter /note=promoter CDS 39..620 /label=SKP1 /note=CDS CDS 621..644 /label=Linker GIPDLGAG /note=CDS CDS 645..668 /label=Linker AGAGDLTS /note=CDS promoter 8077..29 /label=ACS2 promoter /note=promoter CDS 7042..7752 /label=yeGFP /note=codons optimized for translation in S. cerevisiae and C. albicans primer_bind complement(7069..7097) /label=GFP-R /note=GFP, reverse primer. Does NOT anneal to EGFP primer_bind complement(2632..2648) /label=M13 rev /note=common sequencing primer, one of multiple similar variants primer_bind complement(2645..2667) /label=M13/pUC Reverse /note=In lacZ gene protein_bind 2656..2672 /label=lac operator /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). promoter complement(2680..2710) /label=lac promoter /note=promoter for the E. coli lac operon protein_bind 2725..2746 /label=CAP binding site /note=CAP binding activates transcription in the presence of cAMP. primer_bind complement(2863..2880) /label=L4440 /note=L4440 vector, forward primer rep_origin complement(3034..3622) /label=ori /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication primer_bind complement(3114..3133) /label=pBR322ori-F /note=pBR322 origin, forward primer primer_bind 4416..4435 /label=Amp-R /note=Ampicillin resistance gene, reverse primer primer_bind 4826..4844 /label=pBRforEco /note=pBR322 vectors, upsteam of EcoRI site, forward primer primer_bind complement(4882..4904) /label=pGEX 3' /note=pGEX vectors, reverse primer primer_bind 5004..5023 /label=pRS-marker /note=pRS vectors, use to sequence yeast selectable marker primer_bind 5217..5239 /label=M13/pUC Forward /note=In lacZ gene primer_bind 5231..5248 /label=M13 Forward /note=In lacZ gene. Also called M13-F20 or M13 (-21) Forward primer_bind 5232..5248 /label=M13 fwd /note=common sequencing primer, one of multiple similar variants protein_bind 6362..6395 /label=loxP /note=Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT). BASE COUNT 2408 a 1723 c 1886 g 2478 t ORIGIN 1 CAGGAAAGTA AATCAATACA ATAATAAAAG CTAGCAAAAT GGTGACTTCT AATGTTGTCC 61 TAGTGAGTGG TGAGGGTGAA CGATTCACCG TAGACAAGAA AATCGCGGAG AGATCCCTGC 121 TATTGAAAAA CTATCTGAAC GATATGCATG ACAGCAACCT ACAAAACAAC TCAGATTCAG 181 AATCAGATTC AGACTCGGAA ACGAACCACA AAAGCAAAGA CAACAATAAC GGTGACGACG 241 ACGATGAGGA TGACGACGAG ATTGTGATGC CCGTGCCCAA TGTTCGGTCT TCCGTTTTGC 301 AGAAGGTTAT CGAATGGGCC GAGCACCACA GAGACTCTAA CTTCCCTGAC GAAGATGACG 361 ACGATTCCAG AAAGAGTGCT CCCGTGGACT CATGGGACAG AGAATTCCTG AAAGTGGACC 421 AAGAGATGCT ATACGAAATT ATTCTGGCGG CAAACTACCT CAACATCAAG CCCTTGCTTG 481 ACGCTGGCTG CAAGGTGGTT GCTGAAATGA TCAGGGGAAG GTCTCCCGAG GAAATTAGAA 541 GAACGTTTAA TATCGTCAAC GACTTCACTC CAGAGGAGGA GGCTGCCATT AGGCGTGAAA 601 ATGAATGGGC TGAAGACCGT GGTATCTTTG ATTTGGGTGC TGGTGCTGGT GCTGGTGACC 661 TTACTTCTAT GACCTATTTC CCAGAAGAAG TCGTTGAACA CATCTTCTCT TTCTTGCCAG 721 CTCAAAGAGA TAGAAATACT GTTTCCTTGG TTTGTAAGGT TTGGTATGAA ATCGAAAGAT 781 TGAGTAGACG TGGTGTTTTC GTCGGTAATT GTTACGCTGT TAGAGCTGGT AGAGTTGCTG 841 CTAGATTCCC AAATGTTAGA GCCTTGACTG TCAAAGGTAA ACCACATTTT GCTGACTTTA 901 ACTTGGTCCC ACCAGATTGG GGTGGTTATG CTGGTCCATG GATTGAAGCT GCCGCTAGAG 961 GTTGTCACGG TTTGGAAGAA TTGCGTATGA AAAGAATGGT CGTTTCTGAC GAATCTTTGG 1021 AATTATTGGC CCGTTCTTTT CCAAGATTCA GAGCTTTGGT TTTGATCTCT TGTGAAGGTT 1081 TCAGTACTGA CGGTTTGGCT GCCGTTGCTT CCCATTGTAA ATTATTGAGA GAATTGGACT 1141 TGCAAGAAAA TGAAGTCGAA GATAGAGGTC CAAGATGGTT ATCTTGTTTT CCAGACTCTT 1201 GTACTTCTTT GGTCTCTTTG AACTTCGCTT GTATTAAGGG TGAAGTCAAC GCTGGTTCTT 1261 TAGAAAGATT GGTCTCCCGT TCTCCAAACT TACGTTCTTT GAGATTGAAT CGTTCTGTTT 1321 CCGTTGATAC CTTGGCTAAA ATCTTATTGA GAACCCCAAA CTTGGAAGAC TTAGGTACTG 1381 GTAACTTGAC CGACGATTTC CAAACTGAAT CCTACTTTAA ATTAACTTCT GCTTTAGAAA 1441 AGTGTAAGAT GTTGAGATCC TTATCTGGTT TTTGGGACGC CTCTCCAGTC TGTTTATCTT 1501 TTATCTATCC ATTGTGTGCT CAATTAACTG GTTTAAACTT ATCTTACGCT CCAACCTTGG 1561 ATGCTAGTGA CTTAACCAAG ATGATTAGTA GATGTGTTAA GTTACAAAGA TTATGGGTTT 1621 TAGATTGTAT TTCCGATAAG GGTTTACAAG TCGTTGCTAG TAGCTGTAAG GATTTGCAAG 1681 AATTAAGAGT CTTTCCAAGT GATTTTTACG TTGCTGGTTA CAGCGCTGTT ACTGAAGAAG 1741 GTTTGGTCGC TGTTTCTTTG GGTTGTCCAA AGTTGAACAG CTTATTGTAC TTTTGTCATC 1801 AAATGACTAA TGCTGCCTTA GTCACTGTTG CTAAGAACTG TCCAAACTTC ACCCGTTTCA 1861 GATTGTGTAT TTTGGAACCA GGTAAACCAG ACGTTGTCAC CTCCCAACCA TTGGATGAAG 1921 GTTTCGGTGC TATCGTTAGA GAATGTAAGG GTTTGCAAAG ATTATCTATC TCTGGTTTGT 1981 TAACTGACAA AGTTTTCATG TACATCGGTA AATATGCCAA ACAATTAGAA ATGTTGAGCA 2041 TTGCTTTCGC TGGTGACTCT GACAAGGGTA TGATGCACGT TATGAATGGT TGTAAGAATT 2101 TGAGAAAGTT GGAAATTAGA GATTCTCCAT TCGGTGACGC TGCCTTATTG GGTAATTTTG 2161 CTAGATACGA AACTATGAGA AGTTTGTGGA TGAGTTCCTG TAACGTTACC TTGAAAGGTT 2221 GTCAAGTCTT AGCCAGCAAG ATGCCAATGT TGAACGTCGA AGTCATCAAC GAAAGAGACG 2281 GTAGTAACGA AATGGAAGAA AATCACGGTG ACTTACCAAA GGTAGAAAAG TTGTACGTAT 2341 ACAGAACTAC TGCTGGTGCT AGAGATGATG CTCCAAATTT CGTAAAGATT TTGTAACTCG 2401 AGAGGACTAT TTGCAAAGGG AAGGGATGCT AAGGTAGAGG GTGAACGTTA CAGAAAAGCA 2461 GGCTGGGAAG CATATTTGAG AAGATGCGGC CAGCAAAACT AAAAAACTGT ATTATAAGTA 2521 AATGCATGTA TACTAAACTC ACAAATTAGA GCTTCAATTT AATTATATCA GTTATTACCC 2581 GGGAATCTCG GTCGTAATGA TTTAAATGCA TGCAAGCTTG GCGTAATCAT GGTCATAGCT 2641 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 2701 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 2761 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 2821 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 2881 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 2941 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 3001 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 3061 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 3121 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 3181 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 3241 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 3301 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 3361 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 3421 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 3481 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 3541 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 3601 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 3661 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 3721 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 3781 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 3841 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 3901 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 3961 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 4021 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 4081 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 4141 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 4201 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 4261 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 4321 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 4381 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 4441 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 4501 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 4561 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 4621 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 4681 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 4741 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT 4801 TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG 4861 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 4921 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 4981 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 5041 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC 5101 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA 5161 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA 5221 GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCATTTAA ATTTTTTTGA TTCGGTAATC 5281 TCCGAGCAGA AGGAAGAACG AAGGAAGGAG CACAGACTTA GATTGGTATA TATACGCATA 5341 TGTGGTGTTG AAGAAACATG AAATTGCCCA GTATTCTTAA CCCAACTGCA CAGAACAAAA 5401 ACCTGCAGGA AACGAAGATA AAACATGTCC ACAAAATCAT ATACCAGTAG AGCTGAGACT 5461 CATGCAAGTC CGGTTGCATC GAAACTTTTA CGTTTAATGG ATGAAAAGAA GACCAATTTG 5521 TGTGCTTCTC TTGACGTTCG TTCGACTGAT GAGCTATTGA AACTTGTTGA AACGTTGGGT 5581 CCATACATTT GCCTTTTGAA AACACACGTT GATATCTTGG ATGATTTCAG TTATGAGGGT 5641 ACTGTCGTTC CATTGAAAGC ATTGGCAGAG AAATACAAGT TCTTGATATT TGAGGACAGA 5701 AAATTCGCCG ATATCGGTAA CACAGTCAAA TTACAATATA CATCGGGCGT TTACCGTATC 5761 GCAGAATGGT CTGATATCAC CAACGCCCAC GGGGTTACTG GTGCTGGTAT TGTTGCTGGC 5821 TTGAAACAAG GTGCGCAAGA GGTCACCAAA GAACCAAGGG GATTATTGAT GCTTGCTGAA 5881 TTGTCTTCCA AGGGTTCTCT AGCACACGGT GAATATACTA AGGGTACCGT TGATATTGCA 5941 AAGAGTGATA AAGATTTCGT TATTGGGTTC ATTGCTCAGA ACGATATGGG AGGAAGAGAA 6001 GAAGGGTTTG ATTGGCTAAT CATGACCCCA GGTGTAGGTT TAGACGACAA AGGCGATGCA 6061 TTGGGTCAGC AGTACAGAAC CGTCGACGAA GTTGTAAGTG GTGGATCAGA TATCATCATT 6121 GTTGGCAGAG GACTTTTCGC CAAGGGTAGA GATCCTAAGG TTGAAGGTGA AAGATACAGA 6181 AATGCTGGAT GGGAAGCGTA CCAAAAGAGA ATCAGCGCTC CCCATTAATT ATACAGGAAA 6241 CTTAATAGAA CAAATCACAT ATTTAATCTA ATAGCCACCT GCATTGGCAC GGTGCAACAC 6301 TCACTTCAAC TTCATCTTAC AAAAGATCAC GTGATCTGTT GTATTGGGAT CTCTAGACCT 6361 AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTGCTCG ACCAGAAAGC 6421 GACCACCCAA CTTTGGCTGA TAATAGCGTA TAAACAATGC ATACTTTGTA CGTTCAAAAT 6481 ACAATGCAGT AGATATATTT ATGCATATTA CATATAATAC ATATCACATA GGAAGCAACA 6541 GGCGCGTTGG ACTTTTAATT TTCGAGGACC GCGAATCCTT ACATCACACC CAATCCCCCA 6601 CAAGTGATCC CCCACACACC ATAGCTTCAA AATGTTTCTA CTCCTTTTTT ACTCTTCCAG 6661 ATTTTCTCGG ACTCCGCGCA TCGCCGTACC ACTTCAAAAC ACCCAAGCAC AGCATACTAA 6721 ATTTCCCCTC TTTCTTCCTC TAGGGTGTCG TTAATTACCC GTACTAAAGG TTTGGAAAAG 6781 AAAAAAGAGA CCGCCTCGTT TCTTTTTCTT CGTCGAAAAA GGCAATAAAA ATTTTTATCA 6841 CGTTTCTTTT TCTTGAAAAT TTTTTTTTTT GATTTTTTTC TCTTTCGATG ACCTCCCATT 6901 GATATTTAAG TTAATAAACG GTCTTCAATT TCTCAAGTTT CAGTTTCATT TTTCTTGTTC 6961 TATTACAACT TTTTTTACTT CTTGCTCATT AGAAAGAAAG CATAGCAATC TAATCTAAGT 7021 TTTAATTACA AAATGGGATC CTCTAAAGGT GAAGAATTAT TCACTGGTGT TGTCCCAATT 7081 TTGGTTGAAT TAGATGGTGA TGTTAATGGT CACAAATTTT CTGTCTCCGG TGAAGGTGAA 7141 GGTGATGCTA CTTACGGTAA ATTGACCTTA AAATTTATTT GTACTACTGG TAAATTGCCA 7201 GTTCCATGGC CAACCTTAGT CACTACTTTC GGTTATGGTG TTCAATGTTT TGCGAGATAC 7261 CCAGATCATA TGAAACAACA TGACTTTTTC AAGTCTGCCA TGCCAGAAGG TTATGTTCAA 7321 GAAAGAACTA TTTTTTTCAA AGATGACGGT AACTACAAGA CCAGAGCTGA AGTCAAGTTT 7381 GAAGGTGATA CCTTAGTTAA TAGAATCGAA TTAAAAGGTA TTGATTTTAA AGAAGATGGT 7441 AACATTTTAG GTCACAAATT GGAATACAAC TATAACTCTC ACAATGTTTA CATCATGGCT 7501 GACAAACAAA AGAATGGTAT CAAAGTTAAC TTCAAAATTA GACACAACAT TGAAGATGGT 7561 TCTGTTCAAT TAGCTGACCA TTATCAACAA AATACTCCAA TTGGTGATGG TCCAGTCTTG 7621 TTACCAGACA ACCATTACTT ATCCACTCAA TCTGCCTTAT CCAAAGATCC AAACGAAAAG 7681 AGAGACCACA TGGTCTTGTT AGAATTTGTT ACTGCTGCTG GTATTACCCA TGGTATGGAT 7741 GAATTGTACA AAAGATCTTA AACTAGTATT GAATTGAATT GAAATCGATA GATCAATTTT 7801 TTTCTTTTCT CTTTCCCCAT CCTTTACGCT AAAATAATAG TTTATTTTAT TTTTTGAATA 7861 TTTTTTATTT ATATACGTAT ATATAGACTA TTATTTATCT TTTAATGATT ATTAAGATTT 7921 TTATTAAAAA AAAATTCGCT CCTCTTTTAA TGCCTTTATG CAGTTTTTTT TTCCCATTCG 7981 ATATTTCTAT GTTCGGGTTC AGCGTATTTT AAGTTTAATA ACTCGAAAAT TCTGCGTTCG 8041 TTAAAGCTTT CGAGAAGGAT ATTATTTGCG GCCGCGATTG CAAGAATTGA TCTCCCTCAT 8101 CGATGTCATA AATCAGTAAA TATAAACATT AAATACATTA CACGTAGCCA CCCGCTGTAT 8161 AGCATAAAAC TAACTTAAAA TTATTCATAT GCGTTTCCCG GGGCCGAAGC GTTATTGCCG 8221 ATATTCGGTA GCCGATTCCG CTGGCTTTTT TTTTTTGAAC GAGGCAAAAA AGTTTCGTGA 8281 CTGAACTTCT TCGTCACTGG TTTTGTTGAT AATTTTTATC GTTTTTTACT TCGAGATTGA 8341 AAACAGTGTT CTGAATTTTA GCAACGATAT CGCACCGCAC GTTTAGCCTG ATCAAACCTT 8401 ATATATACCT CTACCTTAAA ATATATATAT ATATAGTATT CGAATTCCGA ATTGTGTGTC 8461 TCCGCGGTTA GTGATTGTTA TACACAAACA GAATA //