LOCUS       In-Vivo\Anneal          8210 bp    DNA     circular     06-Dec-2020
FEATURES             Location/Qualifiers
     CDS             5528..6331
                     /label=KlURA3
                     /note=Detected CDS 267aa
     promoter        5362..5527
                     /label=URA3p
                     /note=promoter
     misc_feature    2506..2706
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(3122..3736)
                     /label=pUC
                     /note=pUC Origin
     misc_feature    6550..7135
                     /label=TEF1P
                     /note=misc_feature
     CDS             772..2499
                     /label=OzTIR1
                     /note=CDS
     CDS             complement(3896..4756)
                     /label=bla
                     /note=Ampicilin Resistance
     CDS             7862..7993
                     /label=AID*
                     /note=CDS
     CDS             7994..8176
                     /label=CUP1
                     /note=CDS
     terminator      6..305
                     /label=PGK1 terminator
                     /note=terminator
     promoter        complement(4757..4861)
                     /label=AmpR promoter
                     /note=promoter
     promoter        315..762
                     /label=ACS2 promoter
                     /note=promoter
     CDS             7145..7855
                     /label=yeGFP
                     /note=codons optimized for translation in S. cerevisiae and C. albicans
     primer_bind     complement(7172..7200)
                     /label=GFP-R
                     /note=GFP, reverse primer. Does NOT anneal to EGFP
     primer_bind     complement(2735..2751)
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     primer_bind     complement(2748..2770)
                     /label=M13/pUC Reverse
                     /note=In lacZ gene
     protein_bind    2759..2775
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(2783..2813)
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    2828..2849
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presence of cAMP.
     primer_bind     complement(2966..2983)
                     /label=L4440
                     /note=L4440 vector, forward primer
     rep_origin      complement(3137..3725)
                     /label=ori
                     /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
     primer_bind     complement(3217..3236)
                     /label=pBR322ori-F
                     /note=pBR322 origin, forward primer
     primer_bind     4519..4538
                     /label=Amp-R
                     /note=Ampicillin resistance gene, reverse primer
     primer_bind     4929..4947
                     /label=pBRforEco
                     /note=pBR322 vectors, upsteam of EcoRI site, forward primer
     primer_bind     complement(4985..5007)
                     /label=pGEX 3'
                     /note=pGEX vectors, reverse primer
     primer_bind     5107..5126
                     /label=pRS-marker
                     /note=pRS vectors, use to sequence yeast selectable marker
     primer_bind     5320..5342
                     /label=M13/pUC Forward
                     /note=In lacZ gene
     primer_bind     5334..5351
                     /label=M13 Forward
                     /note=In lacZ gene. Also called M13-F20 or M13 (-21) Forward
     primer_bind     5335..5351
                     /label=M13 fwd
                     /note=common sequencing primer, one of multiple similar variants
     protein_bind    6465..6498
                     /label=loxP
                     /note=Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
     CDS             3666..3674
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(2958..2966)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             772..2496
                     /label=OzTIR1
                     /note=CDS
     CDS             3666..3674
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(2958..2966)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(2958..2966)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             3666..3674
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             8177..8206
                     /label=SV40 NLS CTPPKKKRKV
                     /note=CDS
     CDS             3666..3674
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(2958..2966)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
BASE COUNT     2335 a      1669 c      1799 g      2407 t 
ORIGIN
        1 CTAGTATTGA ATTGAATTGA AATCGATAGA TCAATTTTTT TCTTTTCTCT TTCCCCATCC 
       61 TTTACGCTAA AATAATAGTT TATTTTATTT TTTGAATATT TTTTATTTAT ATACGTATAT 
      121 ATAGACTATT ATTTATCTTT TAATGATTAT TAAGATTTTT ATTAAAAAAA AATTCGCTCC 
      181 TCTTTTAATG CCTTTATGCA GTTTTTTTTT CCCATTCGAT ATTTCTATGT TCGGGTTCAG 
      241 CGTATTTTAA GTTTAATAAC TCGAAAATTC TGCGTTCGTT AAAGCTTTCG AGAAGGATAT 
      301 TATTTGCGGC CGCGATTGCA AGAATTGATC TCCCTCATCG ATGTCATAAA TCAGTAAATA 
      361 TAAACATTAA ATACATTACA CGTAGCCACC CGCTGTATAG CATAAAACTA ACTTAAAATT 
      421 ATTCATATGC GTTTCCCGGG GCCGAAGCGT TATTGCCGAT ATTCGGTAGC CGATTCCGCT 
      481 GGCTTTTTTT TTTTGAACGA GGCAAAAAAG TTTCGTGACT GAACTTCTTC GTCACTGGTT 
      541 TTGTTGATAA TTTTTATCGT TTTTTACTTC GAGATTGAAA ACAGTGTTCT GAATTTTAGC 
      601 AACGATATCG CACCGCACGT TTAGCCTGAT CAAACCTTAT ATATACCTCT ACCTTAAAAT 
      661 ATATATATAT ATAGTATTCG AATTCCGAAT TGTGTGTCTC CGCGGTTAGT GATTGTTATA 
      721 CACAAACAGA ATACAGGAAA GTAAATCAAT ACAATAATAA AAGCTAGCAA AATGACCTAT 
      781 TTCCCAGAAG AAGTCGTTGA ACACATCTTC TCTTTCTTGC CAGCTCAAAG AGATAGAAAT 
      841 ACTGTTTCCT TGGTTTGTAA GGTTTGGTAT GAAATCGAAA GATTGAGTAG ACGTGGTGTT 
      901 TTCGTCGGTA ATTGTTACGC TGTTAGAGCT GGTAGAGTTG CTGCTAGATT CCCAAATGTT 
      961 AGAGCCTTGA CTGTCAAAGG TAAACCACAT TTTGCTGACT TTAACTTGGT CCCACCAGAT 
     1021 TGGGGTGGTT ATGCTGGTCC ATGGATTGAA GCTGCCGCTA GAGGTTGTCA CGGTTTGGAA 
     1081 GAATTGCGTA TGAAAAGAAT GGTCGTTTCT GACGAATCTT TGGAATTATT GGCCCGTTCT 
     1141 TTTCCAAGAT TCAGAGCTTT GGTTTTGATC TCTTGTGAAG GTTTCAGTAC TGACGGTTTG 
     1201 GCTGCCGTTG CTTCCCATTG TAAATTATTG AGAGAATTGG ACTTGCAAGA AAATGAAGTC 
     1261 GAAGATAGAG GTCCAAGATG GTTATCTTGT TTTCCAGACT CTTGTACTTC TTTGGTCTCT 
     1321 TTGAACTTCG CTTGTATTAA GGGTGAAGTC AACGCTGGTT CTTTAGAAAG ATTGGTCTCC 
     1381 CGTTCTCCAA ACTTACGTTC TTTGAGATTG AATCGTTCTG TTTCCGTTGA TACCTTGGCT 
     1441 AAAATCTTAT TGAGAACCCC AAACTTGGAA GACTTAGGTA CTGGTAACTT GACCGACGAT 
     1501 TTCCAAACTG AATCCTACTT TAAATTAACT TCTGCTTTAG AAAAGTGTAA GATGTTGAGA 
     1561 TCCTTATCTG GTTTTTGGGA CGCCTCTCCA GTCTGTTTAT CTTTTATCTA TCCATTGTGT 
     1621 GCTCAATTAA CTGGTTTAAA CTTATCTTAC GCTCCAACCT TGGATGCTAG TGACTTAACC 
     1681 AAGATGATTA GTAGATGTGT TAAGTTACAA AGATTATGGG TTTTAGATTG TATTTCCGAT 
     1741 AAGGGTTTAC AAGTCGTTGC TAGTAGCTGT AAGGATTTGC AAGAATTAAG AGTCTTTCCA 
     1801 AGTGATTTTT ACGTTGCTGG TTACAGCGCT GTTACTGAAG AAGGTTTGGT CGCTGTTTCT 
     1861 TTGGGTTGTC CAAAGTTGAA CAGCTTATTG TACTTTTGTC ATCAAATGAC TAATGCTGCC 
     1921 TTAGTCACTG TTGCTAAGAA CTGTCCAAAC TTCACCCGTT TCAGATTGTG TATTTTGGAA 
     1981 CCAGGTAAAC CAGACGTTGT CACCTCCCAA CCATTGGATG AAGGTTTCGG TGCTATCGTT 
     2041 AGAGAATGTA AGGGTTTGCA AAGATTATCT ATCTCTGGTT TGTTAACTGA CAAAGTTTTC 
     2101 ATGTACATCG GTAAATATGC CAAACAATTA GAAATGTTGA GCATTGCTTT CGCTGGTGAC 
     2161 TCTGACAAGG GTATGATGCA CGTTATGAAT GGTTGTAAGA ATTTGAGAAA GTTGGAAATT 
     2221 AGAGATTCTC CATTCGGTGA CGCTGCCTTA TTGGGTAATT TTGCTAGATA CGAAACTATG 
     2281 AGAAGTTTGT GGATGAGTTC CTGTAACGTT ACCTTGAAAG GTTGTCAAGT CTTAGCCAGC 
     2341 AAGATGCCAA TGTTGAACGT CGAAGTCATC AACGAAAGAG ACGGTAGTAA CGAAATGGAA 
     2401 GAAAATCACG GTGACTTACC AAAGGTAGAA AAGTTGTACG TATACAGAAC TACTGCTGGT 
     2461 GCTAGAGATG ATGCTCCAAA TTTCGTAAAG ATTTTGTAAC TCGAGAGGAC TATTTGCAAA 
     2521 GGGAAGGGAT GCTAAGGTAG AGGGTGAACG TTACAGAAAA GCAGGCTGGG AAGCATATTT 
     2581 GAGAAGATGC GGCCAGCAAA ACTAAAAAAC TGTATTATAA GTAAATGCAT GTATACTAAA 
     2641 CTCACAAATT AGAGCTTCAA TTTAATTATA TCAGTTATTA CCCGGGAATC TCGGTCGTAA 
     2701 TGATTTAAAT GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     2761 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     2821 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     2881 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     2941 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     3001 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     3061 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     3121 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     3181 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     3241 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     3301 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     3361 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     3421 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     3481 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     3541 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 
     3601 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     3661 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     3721 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     3781 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     3841 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     3901 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     3961 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     4021 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     4081 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     4141 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     4201 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     4261 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     4321 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     4381 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     4441 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     4501 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     4561 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     4621 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     4681 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     4741 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     4801 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     4861 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA 
     4921 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG 
     4981 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG 
     5041 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA 
     5101 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 
     5161 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT 
     5221 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT 
     5281 GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA 
     5341 CGACGGCCAG TGAATTCATT TAAATTTTTT TGATTCGGTA ATCTCCGAGC AGAAGGAAGA 
     5401 ACGAAGGAAG GAGCACAGAC TTAGATTGGT ATATATACGC ATATGTGGTG TTGAAGAAAC 
     5461 ATGAAATTGC CCAGTATTCT TAACCCAACT GCACAGAACA AAAACCTGCA GGAAACGAAG 
     5521 ATAAAACATG TCCACAAAAT CATATACCAG TAGAGCTGAG ACTCATGCAA GTCCGGTTGC 
     5581 ATCGAAACTT TTACGTTTAA TGGATGAAAA GAAGACCAAT TTGTGTGCTT CTCTTGACGT 
     5641 TCGTTCGACT GATGAGCTAT TGAAACTTGT TGAAACGTTG GGTCCATACA TTTGCCTTTT 
     5701 GAAAACACAC GTTGATATCT TGGATGATTT CAGTTATGAG GGTACTGTCG TTCCATTGAA 
     5761 AGCATTGGCA GAGAAATACA AGTTCTTGAT ATTTGAGGAC AGAAAATTCG CCGATATCGG 
     5821 TAACACAGTC AAATTACAAT ATACATCGGG CGTTTACCGT ATCGCAGAAT GGTCTGATAT 
     5881 CACCAACGCC CACGGGGTTA CTGGTGCTGG TATTGTTGCT GGCTTGAAAC AAGGTGCGCA 
     5941 AGAGGTCACC AAAGAACCAA GGGGATTATT GATGCTTGCT GAATTGTCTT CCAAGGGTTC 
     6001 TCTAGCACAC GGTGAATATA CTAAGGGTAC CGTTGATATT GCAAAGAGTG ATAAAGATTT 
     6061 CGTTATTGGG TTCATTGCTC AGAACGATAT GGGAGGAAGA GAAGAAGGGT TTGATTGGCT 
     6121 AATCATGACC CCAGGTGTAG GTTTAGACGA CAAAGGCGAT GCATTGGGTC AGCAGTACAG 
     6181 AACCGTCGAC GAAGTTGTAA GTGGTGGATC AGATATCATC ATTGTTGGCA GAGGACTTTT 
     6241 CGCCAAGGGT AGAGATCCTA AGGTTGAAGG TGAAAGATAC AGAAATGCTG GATGGGAAGC 
     6301 GTACCAAAAG AGAATCAGCG CTCCCCATTA ATTATACAGG AAACTTAATA GAACAAATCA 
     6361 CATATTTAAT CTAATAGCCA CCTGCATTGG CACGGTGCAA CACTCACTTC AACTTCATCT 
     6421 TACAAAAGAT CACGTGATCT GTTGTATTGG GATCTCTAGA CCTAATAACT TCGTATAGCA 
     6481 TACATTATAC GAAGTTATAT TAAGGGTTGC TCGACCAGAA AGCGACCACC CAACTTTGGC 
     6541 TGATAATAGC GTATAAACAA TGCATACTTT GTACGTTCAA AATACAATGC AGTAGATATA 
     6601 TTTATGCATA TTACATATAA TACATATCAC ATAGGAAGCA ACAGGCGCGT TGGACTTTTA 
     6661 ATTTTCGAGG ACCGCGAATC CTTACATCAC ACCCAATCCC CCACAAGTGA TCCCCCACAC 
     6721 ACCATAGCTT CAAAATGTTT CTACTCCTTT TTTACTCTTC CAGATTTTCT CGGACTCCGC 
     6781 GCATCGCCGT ACCACTTCAA AACACCCAAG CACAGCATAC TAAATTTCCC CTCTTTCTTC 
     6841 CTCTAGGGTG TCGTTAATTA CCCGTACTAA AGGTTTGGAA AAGAAAAAAG AGACCGCCTC 
     6901 GTTTCTTTTT CTTCGTCGAA AAAGGCAATA AAAATTTTTA TCACGTTTCT TTTTCTTGAA 
     6961 AATTTTTTTT TTTGATTTTT TTCTCTTTCG ATGACCTCCC ATTGATATTT AAGTTAATAA 
     7021 ACGGTCTTCA ATTTCTCAAG TTTCAGTTTC ATTTTTCTTG TTCTATTACA ACTTTTTTTA 
     7081 CTTCTTGCTC ATTAGAAAGA AAGCATAGCA ATCTAATCTA AGTTTTAATT ACAAAATGGG 
     7141 ATCCTCTAAA GGTGAAGAAT TATTCACTGG TGTTGTCCCA ATTTTGGTTG AATTAGATGG 
     7201 TGATGTTAAT GGTCACAAAT TTTCTGTCTC CGGTGAAGGT GAAGGTGATG CTACTTACGG 
     7261 TAAATTGACC TTAAAATTTA TTTGTACTAC TGGTAAATTG CCAGTTCCAT GGCCAACCTT 
     7321 AGTCACTACT TTCGGTTATG GTGTTCAATG TTTTGCGAGA TACCCAGATC ATATGAAACA 
     7381 ACATGACTTT TTCAAGTCTG CCATGCCAGA AGGTTATGTT CAAGAAAGAA CTATTTTTTT 
     7441 CAAAGATGAC GGTAACTACA AGACCAGAGC TGAAGTCAAG TTTGAAGGTG ATACCTTAGT 
     7501 TAATAGAATC GAATTAAAAG GTATTGATTT TAAAGAAGAT GGTAACATTT TAGGTCACAA 
     7561 ATTGGAATAC AACTATAACT CTCACAATGT TTACATCATG GCTGACAAAC AAAAGAATGG 
     7621 TATCAAAGTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGTTCTGTTC AATTAGCTGA 
     7681 CCATTATCAA CAAAATACTC CAATTGGTGA TGGTCCAGTC TTGTTACCAG ACAACCATTA 
     7741 CTTATCCACT CAATCTGCCT TATCCAAAGA TCCAAACGAA AAGAGAGACC ACATGGTCTT 
     7801 GTTAGAATTT GTTACTGCTG CTGGTATTAC CCATGGTATG GATGAATTGT ACAAAAGATC 
     7861 TCCAAAAGAC CCAGCTAAAC CTCCAGCCAA GGCCCAAGTC GTCGGCTGGC CACCAGTTAG 
     7921 GTCTTACAGA AAGAACGTAA TGGTTAGTTG TCAAAAGTCC TCCGGTGGTC CAGAAGCTGC 
     7981 TGCCTTTGTT AAGATGTTCA GCGAATTAAT TAACTTCCAA AATGAAGGTC ATGAGTGCCA 
     8041 ATGCCAATGT GGTAGCTGCA AAAATAATGA ACAATGCCAA AAATCATGTA GCTGCCCAAC 
     8101 GGGGTGTAAC AGCGACGACA AATGCCCCTG CGGTAACAAG TCTGAAGAAA CCAAGAAGTC 
     8161 ATGCTGCTCT GGGAAATGTA CCCCACCTAA GAAGAAAAGA AAGGTTTAAA  
//