LOCUS       pILGFP5V1               5879 bp    DNA     circular     07-Dec-2020
FEATURES             Location/Qualifiers
     CDS             2911..3714
                     /label=KlURA3
                     /note=Detected CDS 267aa
     CDS             4843..5571
                     /label=yEGFP
                     /note=Detected CDS 242aa
     promoter        2745..2910
                     /label=URA3p
                     /note=promoter
     rep_origin      complement(505..1119)
                     /label=pUC
                     /note=pUC Origin
     misc_feature    3933..4518
                     /label=TEF1P
                     /note=misc_feature
     CDS             complement(1279..2139)
                     /label=bla
                     /note=Ampicilin Resistance
     CDS             4711..4842
                     /label=AID*
                     /note=CDS
     CDS             4528..4710
                     /label=CUP1
                     /note=CDS
     terminator      5578..5877
                     /label=PGK1 terminator
                     /note=terminator
     promoter        complement(2140..2244)
                     /label=AmpR promoter
                     /note=promoter
     CDS             4852..5562
                     /label=yeGFP
                     /note=codons optimized for translation in S. cerevisiae and C. albicans
     primer_bind     complement(4879..4907)
                     /label=GFP-R
                     /note=GFP, reverse primer. Does NOT anneal to EGFP
     primer_bind     complement(118..134)
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     primer_bind     complement(131..153)
                     /label=M13/pUC Reverse
                     /note=In lacZ gene
     protein_bind    142..158
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(166..196)
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    211..232
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presence of cAMP.
     primer_bind     complement(349..366)
                     /label=L4440
                     /note=L4440 vector, forward primer
     rep_origin      complement(520..1108)
                     /label=ori
                     /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
     primer_bind     complement(600..619)
                     /label=pBR322ori-F
                     /note=pBR322 origin, forward primer
     primer_bind     1902..1921
                     /label=Amp-R
                     /note=Ampicillin resistance gene, reverse primer
     primer_bind     2312..2330
                     /label=pBRforEco
                     /note=pBR322 vectors, upsteam of EcoRI site, forward primer
     primer_bind     complement(2368..2390)
                     /label=pGEX 3'
                     /note=pGEX vectors, reverse primer
     primer_bind     2490..2509
                     /label=pRS-marker
                     /note=pRS vectors, use to sequence yeast selectable marker
     primer_bind     2703..2725
                     /label=M13/pUC Forward
                     /note=In lacZ gene
     primer_bind     2717..2734
                     /label=M13 Forward
                     /note=In lacZ gene. Also called M13-F20 or M13 (-21) Forward
     primer_bind     2718..2734
                     /label=M13 fwd
                     /note=common sequencing primer, one of multiple similar variants
     protein_bind    3848..3881
                     /label=loxP
                     /note=Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
     CDS             1049..1057
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(341..349)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             1049..1057
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(341..349)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(341..349)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             1049..1057
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             1049..1057
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(341..349)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     misc_feature    1..89
                     /label=URA3 terminator region
                     /note=misc_feature
BASE COUNT     1677 a      1267 c      1285 g      1650 t 
ORIGIN
        1 TAAGTAAATG CATGTATACT AAACTCACAA ATTAGAGCTT CAATTTAATT ATATCAGTTA 
       61 TTACCCGGGA ATCTCGGTCG TAATGATTTA AATGCATGCA AGCTTGGCGT AATCATGGTC 
      121 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 
      181 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 
      241 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 
      301 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 
      361 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
      421 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
      481 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
      541 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
      601 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
      661 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
      721 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
      781 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
      841 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
      901 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
      961 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     1021 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     1081 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     1141 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     1201 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     1261 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 
     1321 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 
     1381 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 
     1441 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 
     1501 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 
     1561 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 
     1621 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 
     1681 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 
     1741 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 
     1801 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 
     1861 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 
     1921 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 
     1981 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 
     2041 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 
     2101 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 
     2161 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 
     2221 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA 
     2281 AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT 
     2341 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC 
     2401 AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT 
     2461 TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA 
     2521 CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA 
     2581 TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT 
     2641 ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT 
     2701 TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC ATTTAAATTT TTTTGATTCG 
     2761 GTAATCTCCG AGCAGAAGGA AGAACGAAGG AAGGAGCACA GACTTAGATT GGTATATATA 
     2821 CGCATATGTG GTGTTGAAGA AACATGAAAT TGCCCAGTAT TCTTAACCCA ACTGCACAGA 
     2881 ACAAAAACCT GCAGGAAACG AAGATAAAAC ATGTCCACAA AATCATATAC CAGTAGAGCT 
     2941 GAGACTCATG CAAGTCCGGT TGCATCGAAA CTTTTACGTT TAATGGATGA AAAGAAGACC 
     3001 AATTTGTGTG CTTCTCTTGA CGTTCGTTCG ACTGATGAGC TATTGAAACT TGTTGAAACG 
     3061 TTGGGTCCAT ACATTTGCCT TTTGAAAACA CACGTTGATA TCTTGGATGA TTTCAGTTAT 
     3121 GAGGGTACTG TCGTTCCATT GAAAGCATTG GCAGAGAAAT ACAAGTTCTT GATATTTGAG 
     3181 GACAGAAAAT TCGCCGATAT CGGTAACACA GTCAAATTAC AATATACATC GGGCGTTTAC 
     3241 CGTATCGCAG AATGGTCTGA TATCACCAAC GCCCACGGGG TTACTGGTGC TGGTATTGTT 
     3301 GCTGGCTTGA AACAAGGTGC GCAAGAGGTC ACCAAAGAAC CAAGGGGATT ATTGATGCTT 
     3361 GCTGAATTGT CTTCCAAGGG TTCTCTAGCA CACGGTGAAT ATACTAAGGG TACCGTTGAT 
     3421 ATTGCAAAGA GTGATAAAGA TTTCGTTATT GGGTTCATTG CTCAGAACGA TATGGGAGGA 
     3481 AGAGAAGAAG GGTTTGATTG GCTAATCATG ACCCCAGGTG TAGGTTTAGA CGACAAAGGC 
     3541 GATGCATTGG GTCAGCAGTA CAGAACCGTC GACGAAGTTG TAAGTGGTGG ATCAGATATC 
     3601 ATCATTGTTG GCAGAGGACT TTTCGCCAAG GGTAGAGATC CTAAGGTTGA AGGTGAAAGA 
     3661 TACAGAAATG CTGGATGGGA AGCGTACCAA AAGAGAATCA GCGCTCCCCA TTAATTATAC 
     3721 AGGAAACTTA ATAGAACAAA TCACATATTT AATCTAATAG CCACCTGCAT TGGCACGGTG 
     3781 CAACACTCAC TTCAACTTCA TCTTACAAAA GATCACGTGA TCTGTTGTAT TGGGATCTCT 
     3841 AGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TATTAAGGGT TGCTCGACCA 
     3901 GAAAGCGACC ACCCAACTTT GGCTGATAAT AGCGTATAAA CAATGCATAC TTTGTACGTT 
     3961 CAAAATACAA TGCAGTAGAT ATATTTATGC ATATTACATA TAATACATAT CACATAGGAA 
     4021 GCAACAGGCG CGTTGGACTT TTAATTTTCG AGGACCGCGA ATCCTTACAT CACACCCAAT 
     4081 CCCCCACAAG TGATCCCCCA CACACCATAG CTTCAAAATG TTTCTACTCC TTTTTTACTC 
     4141 TTCCAGATTT TCTCGGACTC CGCGCATCGC CGTACCACTT CAAAACACCC AAGCACAGCA 
     4201 TACTAAATTT CCCCTCTTTC TTCCTCTAGG GTGTCGTTAA TTACCCGTAC TAAAGGTTTG 
     4261 GAAAAGAAAA AAGAGACCGC CTCGTTTCTT TTTCTTCGTC GAAAAAGGCA ATAAAAATTT 
     4321 TTATCACGTT TCTTTTTCTT GAAAATTTTT TTTTTTGATT TTTTTCTCTT TCGATGACCT 
     4381 CCCATTGATA TTTAAGTTAA TAAACGGTCT TCAATTTCTC AAGTTTCAGT TTCATTTTTC 
     4441 TTGTTCTATT ACAACTTTTT TTACTTCTTG CTCATTAGAA AGAAAGCATA GCAATCTAAT 
     4501 CTAAGTTTTA ATTACAAACT CGAGAAAATG TTCAGCGAAT TAATTAACTT CCAAAATGAA 
     4561 GGTCATGAGT GCCAATGCCA ATGTGGTAGC TGCAAAAATA ATGAACAATG CCAAAAATCA 
     4621 TGTAGCTGCC CAACGGGGTG TAACAGCGAC GACAAATGCC CCTGCGGTAA CAAGTCTGAA 
     4681 GAAACCAAGA AGTCATGCTG CTCTGGGAAA CCAAAAGACC CAGCTAAACC TCCAGCCAAG 
     4741 GCCCAAGTCG TCGGCTGGCC ACCAGTTAGG TCTTACAGAA AGAACGTAAT GGTTAGTTGT 
     4801 CAAAAGTCCT CCGGTGGTCC AGAAGCTGCT GCCTTTGTTA AGATGGGATC CTCTAAAGGT 
     4861 GAAGAATTAT TCACTGGTGT TGTCCCAATT TTGGTTGAAT TAGATGGTGA TGTTAATGGT 
     4921 CACAAATTTT CTGTCTCCGG TGAAGGTGAA GGTGATGCTA CTTACGGTAA ATTGACCTTA 
     4981 AAATTTATTT GTACTACTGG TAAATTGCCA GTTCCATGGC CAACCTTAGT CACTACTTTC 
     5041 GGTTATGGTG TTCAATGTTT TGCGAGATAC CCAGATCATA TGAAACAACA TGACTTTTTC 
     5101 AAGTCTGCCA TGCCAGAAGG TTATGTTCAA GAAAGAACTA TTTTTTTCAA AGATGACGGT 
     5161 AACTACAAGA CCAGAGCTGA AGTCAAGTTT GAAGGTGATA CCTTAGTTAA TAGAATCGAA 
     5221 TTAAAAGGTA TTGATTTTAA AGAAGATGGT AACATTTTAG GTCACAAATT GGAATACAAC 
     5281 TATAACTCTC ACAATGTTTA CATCATGGCT GACAAACAAA AGAATGGTAT CAAAGTTAAC 
     5341 TTCAAAATTA GACACAACAT TGAAGATGGT TCTGTTCAAT TAGCTGACCA TTATCAACAA 
     5401 AATACTCCAA TTGGTGATGG TCCAGTCTTG TTACCAGACA ACCATTACTT ATCCACTCAA 
     5461 TCTGCCTTAT CCAAAGATCC AAACGAAAAG AGAGACCACA TGGTCTTGTT AGAATTTGTT 
     5521 ACTGCTGCTG GTATTACCCA TGGTATGGAT GAATTGTACA AAAGATCTTA AACTAGTATT 
     5581 GAATTGAATT GAAATCGATA GATCAATTTT TTTCTTTTCT CTTTCCCCAT CCTTTACGCT 
     5641 AAAATAATAG TTTATTTTAT TTTTTGAATA TTTTTTATTT ATATACGTAT ATATAGACTA 
     5701 TTATTTATCT TTTAATGATT ATTAAGATTT TTATTAAAAA AAAATTCGCT CCTCTTTTAA 
     5761 TGCCTTTATG CAGTTTTTTT TTCCCATTCG ATATTTCTAT GTTCGGGTTC AGCGTATTTT 
     5821 AAGTTTAATA ACTCGAAAAT TCTGCGTTCG TTAAAGCTTT CGAGAAGGAT ATTATTTGC 
//