LOCUS       MultiLig7085            7085 bp    DNA     circular     28-Mar-2019
FEATURES             Location/Qualifiers
     CDS             6154..6957
                     /label=KlURA3
                     /note=Detected CDS 267aa
     promoter        5988..6153
                     /label=URA3p
                     /note=promoter
     misc_feature    3132..3332
                     /label=URA3(705, 905)
                     /note=misc_feature
     rep_origin      complement(3748..4362)
                     /label=pUC
                     /note=pUC Origin
     CDS             1398..3125
                     /label=OzTIR1
                     /note=CDS
     CDS             complement(4522..5382)
                     /label=bla
                     /note=Ampicilin Resistance
     terminator      2..301
                     /label=PGK1 terminator
                     /note=terminator
     promoter        complement(5383..5487)
                     /label=AmpR promoter
                     /note=promoter
     CDS             768..1349
                     /label=SKP1
                     /note=CDS
     CDS             1350..1373
                     /label=Linker GIPDLGAG
                     /note=CDS
     CDS             1374..1397
                     /label=Linker AGAGDLTS
                     /note=CDS
     promoter        311..758
                     /label=ACS2 promoter
                     /note=promoter
     primer_bind     complement(3361..3377)
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     primer_bind     complement(3374..3396)
                     /label=M13/pUC Reverse
                     /note=In lacZ gene
     protein_bind    3385..3401
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(3409..3439)
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    3454..3475
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presence of cAMP.
     primer_bind     complement(3592..3609)
                     /label=L4440
                     /note=L4440 vector, forward primer
     rep_origin      complement(3763..4351)
                     /label=ori
                     /note=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
     primer_bind     complement(3843..3862)
                     /label=pBR322ori-F
                     /note=pBR322 origin, forward primer
     primer_bind     5145..5164
                     /label=Amp-R
                     /note=Ampicillin resistance gene, reverse primer
     primer_bind     5555..5573
                     /label=pBRforEco
                     /note=pBR322 vectors, upsteam of EcoRI site, forward primer
     primer_bind     complement(5611..5633)
                     /label=pGEX 3'
                     /note=pGEX vectors, reverse primer
     primer_bind     5733..5752
                     /label=pRS-marker
                     /note=pRS vectors, use to sequence yeast selectable marker
     primer_bind     5946..5968
                     /label=M13/pUC Forward
                     /note=In lacZ gene
     primer_bind     5960..5977
                     /label=M13 Forward
                     /note=In lacZ gene. Also called M13-F20 or M13 (-21) Forward
     primer_bind     5961..5977
                     /label=M13 fwd
                     /note=common sequencing primer, one of multiple similar variants
     CDS             4292..4300
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(3584..3592)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             1398..3122
                     /label=OzTIR1
                     /note=CDS
     CDS             4292..4300
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             complement(3584..3592)
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
     CDS             complement(3584..3592)
                     /label=Linker GSG(CDS)
                     /note=CDS
     CDS             4292..4300
                     /label=Linker GSG(CDS)(CDS)
                     /note=CDS
BASE COUNT     1963 a      1446 c      1659 g      2017 t 
ORIGIN
        1 TATTGAATTG AATTGAAATC GATAGATCAA TTTTTTTCTT TTCTCTTTCC CCATCCTTTA 
       61 CGCTAAAATA ATAGTTTATT TTATTTTTTG AATATTTTTT ATTTATATAC GTATATATAG 
      121 ACTATTATTT ATCTTTTAAT GATTATTAAG ATTTTTATTA AAAAAAAATT CGCTCCTCTT 
      181 TTAATGCCTT TATGCAGTTT TTTTTTCCCA TTCGATATTT CTATGTTCGG GTTCAGCGTA 
      241 TTTTAAGTTT AATAACTCGA AAATTCTGCG TTCGTTAAAG CTTTCGAGAA GGATATTATT 
      301 TGCGGCCGCG ATTGCAAGAA TTGATCTCCC TCATCGATGT CATAAATCAG TAAATATAAA 
      361 CATTAAATAC ATTACACGTA GCCACCCGCT GTATAGCATA AAACTAACTT AAAATTATTC 
      421 ATATGCGTTT CCCGGGGCCG AAGCGTTATT GCCGATATTC GGTAGCCGAT TCCGCTGGCT 
      481 TTTTTTTTTT GAACGAGGCA AAAAAGTTTC GTGACTGAAC TTCTTCGTCA CTGGTTTTGT 
      541 TGATAATTTT TATCGTTTTT TACTTCGAGA TTGAAAACAG TGTTCTGAAT TTTAGCAACG 
      601 ATATCGCACC GCACGTTTAG CCTGATCAAA CCTTATATAT ACCTCTACCT TAAAATATAT 
      661 ATATATATAG TATTCGAATT CCGAATTGTG TGTCTCCGCG GTTAGTGATT GTTATACACA 
      721 AACAGAATAC AGGAAAGTAA ATCAATACAA TAATAAAAGC TAGCAAAATG GTGACTTCTA 
      781 ATGTTGTCCT AGTGAGTGGT GAGGGTGAAC GATTCACCGT AGACAAGAAA ATCGCGGAGA 
      841 GATCCCTGCT ATTGAAAAAC TATCTGAACG ATATGCATGA CAGCAACCTA CAAAACAACT 
      901 CAGATTCAGA ATCAGATTCA GACTCGGAAA CGAACCACAA AAGCAAAGAC AACAATAACG 
      961 GTGACGACGA CGATGAGGAT GACGACGAGA TTGTGATGCC CGTGCCCAAT GTTCGGTCTT 
     1021 CCGTTTTGCA GAAGGTTATC GAATGGGCCG AGCACCACAG AGACTCTAAC TTCCCTGACG 
     1081 AAGATGACGA CGATTCCAGA AAGAGTGCTC CCGTGGACTC ATGGGACAGA GAATTCCTGA 
     1141 AAGTGGACCA AGAGATGCTA TACGAAATTA TTCTGGCGGC AAACTACCTC AACATCAAGC 
     1201 CCTTGCTTGA CGCTGGCTGC AAGGTGGTTG CTGAAATGAT CAGGGGAAGG TCTCCCGAGG 
     1261 AAATTAGAAG AACGTTTAAT ATCGTCAACG ACTTCACTCC AGAGGAGGAG GCTGCCATTA 
     1321 GGCGTGAAAA TGAATGGGCT GAAGACCGTG GTATCTTTGA TTTGGGTGCT GGTGCTGGTG 
     1381 CTGGTGACCT TACTTCTATG ACCTATTTCC CAGAAGAAGT CGTTGAACAC ATCTTCTCTT 
     1441 TCTTGCCAGC TCAAAGAGAT AGAAATACTG TTTCCTTGGT TTGTAAGGTT TGGTATGAAA 
     1501 TCGAAAGATT GAGTAGACGT GGTGTTTTCG TCGGTAATTG TTACGCTGTT AGAGCTGGTA 
     1561 GAGTTGCTGC TAGATTCCCA AATGTTAGAG CCTTGACTGT CAAAGGTAAA CCACATTTTG 
     1621 CTGACTTTAA CTTGGTCCCA CCAGATTGGG GTGGTTATGC TGGTCCATGG ATTGAAGCTG 
     1681 CCGCTAGAGG TTGTCACGGT TTGGAAGAAT TGCGTATGAA AAGAATGGTC GTTTCTGACG 
     1741 AATCTTTGGA ATTATTGGCC CGTTCTTTTC CAAGATTCAG AGCTTTGGTT TTGATCTCTT 
     1801 GTGAAGGTTT CAGTACTGAC GGTTTGGCTG CCGTTGCTTC CCATTGTAAA TTATTGAGAG 
     1861 AATTGGACTT GCAAGAAAAT GAAGTCGAAG ATAGAGGTCC AAGATGGTTA TCTTGTTTTC 
     1921 CAGACTCTTG TACTTCTTTG GTCTCTTTGA ACTTCGCTTG TATTAAGGGT GAAGTCAACG 
     1981 CTGGTTCTTT AGAAAGATTG GTCTCCCGTT CTCCAAACTT ACGTTCTTTG AGATTGAATC 
     2041 GTTCTGTTTC CGTTGATACC TTGGCTAAAA TCTTATTGAG AACCCCAAAC TTGGAAGACT 
     2101 TAGGTACTGG TAACTTGACC GACGATTTCC AAACTGAATC CTACTTTAAA TTAACTTCTG 
     2161 CTTTAGAAAA GTGTAAGATG TTGAGATCCT TATCTGGTTT TTGGGACGCC TCTCCAGTCT 
     2221 GTTTATCTTT TATCTATCCA TTGTGTGCTC AATTAACTGG TTTAAACTTA TCTTACGCTC 
     2281 CAACCTTGGA TGCTAGTGAC TTAACCAAGA TGATTAGTAG ATGTGTTAAG TTACAAAGAT 
     2341 TATGGGTTTT AGATTGTATT TCCGATAAGG GTTTACAAGT CGTTGCTAGT AGCTGTAAGG 
     2401 ATTTGCAAGA ATTAAGAGTC TTTCCAAGTG ATTTTTACGT TGCTGGTTAC AGCGCTGTTA 
     2461 CTGAAGAAGG TTTGGTCGCT GTTTCTTTGG GTTGTCCAAA GTTGAACAGC TTATTGTACT 
     2521 TTTGTCATCA AATGACTAAT GCTGCCTTAG TCACTGTTGC TAAGAACTGT CCAAACTTCA 
     2581 CCCGTTTCAG ATTGTGTATT TTGGAACCAG GTAAACCAGA CGTTGTCACC TCCCAACCAT 
     2641 TGGATGAAGG TTTCGGTGCT ATCGTTAGAG AATGTAAGGG TTTGCAAAGA TTATCTATCT 
     2701 CTGGTTTGTT AACTGACAAA GTTTTCATGT ACATCGGTAA ATATGCCAAA CAATTAGAAA 
     2761 TGTTGAGCAT TGCTTTCGCT GGTGACTCTG ACAAGGGTAT GATGCACGTT ATGAATGGTT 
     2821 GTAAGAATTT GAGAAAGTTG GAAATTAGAG ATTCTCCATT CGGTGACGCT GCCTTATTGG 
     2881 GTAATTTTGC TAGATACGAA ACTATGAGAA GTTTGTGGAT GAGTTCCTGT AACGTTACCT 
     2941 TGAAAGGTTG TCAAGTCTTA GCCAGCAAGA TGCCAATGTT GAACGTCGAA GTCATCAACG 
     3001 AAAGAGACGG TAGTAACGAA ATGGAAGAAA ATCACGGTGA CTTACCAAAG GTAGAAAAGT 
     3061 TGTACGTATA CAGAACTACT GCTGGTGCTA GAGATGATGC TCCAAATTTC GTAAAGATTT 
     3121 TGTAACTCGA GAGGACTATT TGCAAAGGGA AGGGATGCTA AGGTAGAGGG TGAACGTTAC 
     3181 AGAAAAGCAG GCTGGGAAGC ATATTTGAGA AGATGCGGCC AGCAAAACTA AAAAACTGTA 
     3241 TTATAAGTAA ATGCATGTAT ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG 
     3301 TTATTACCCG GGAATCTCGG TCGTAATGAT TTAAATGCAT GCAAGCTTGG CGTAATCATG 
     3361 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC 
     3421 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC 
     3481 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT 
     3541 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 
     3601 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     3661 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     3721 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     3781 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     3841 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     3901 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     3961 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4021 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4081 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4141 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4201 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4261 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4321 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4381 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4441 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4501 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 
     4561 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 
     4621 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC 
     4681 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 
     4741 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 
     4801 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 
     4861 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 
     4921 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 
     4981 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 
     5041 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 
     5101 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 
     5161 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 
     5221 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 
     5281 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 
     5341 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 
     5401 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 
     5461 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA 
     5521 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG 
     5581 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 
     5641 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 
     5701 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 
     5761 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 
     5821 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 
     5881 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 
     5941 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCATTTAAA TTTTTTTGAT 
     6001 TCGGTAATCT CCGAGCAGAA GGAAGAACGA AGGAAGGAGC ACAGACTTAG ATTGGTATAT 
     6061 ATACGCATAT GTGGTGTTGA AGAAACATGA AATTGCCCAG TATTCTTAAC CCAACTGCAC 
     6121 AGAACAAAAA CCTGCAGGAA ACGAAGATAA AACATGTCCA CAAAATCATA TACCAGTAGA 
     6181 GCTGAGACTC ATGCAAGTCC GGTTGCATCG AAACTTTTAC GTTTAATGGA TGAAAAGAAG 
     6241 ACCAATTTGT GTGCTTCTCT TGACGTTCGT TCGACTGATG AGCTATTGAA ACTTGTTGAA 
     6301 ACGTTGGGTC CATACATTTG CCTTTTGAAA ACACACGTTG ATATCTTGGA TGATTTCAGT 
     6361 TATGAGGGTA CTGTCGTTCC ATTGAAAGCA TTGGCAGAGA AATACAAGTT CTTGATATTT 
     6421 GAGGACAGAA AATTCGCCGA TATCGGTAAC ACAGTCAAAT TACAATATAC ATCGGGCGTT 
     6481 TACCGTATCG CAGAATGGTC TGATATCACC AACGCCCACG GGGTTACTGG TGCTGGTATT 
     6541 GTTGCTGGCT TGAAACAAGG TGCGCAAGAG GTCACCAAAG AACCAAGGGG ATTATTGATG 
     6601 CTTGCTGAAT TGTCTTCCAA GGGTTCTCTA GCACACGGTG AATATACTAA GGGTACCGTT 
     6661 GATATTGCAA AGAGTGATAA AGATTTCGTT ATTGGGTTCA TTGCTCAGAA CGATATGGGA 
     6721 GGAAGAGAAG AAGGGTTTGA TTGGCTAATC ATGACCCCAG GTGTAGGTTT AGACGACAAA 
     6781 GGCGATGCAT TGGGTCAGCA GTACAGAACC GTCGACGAAG TTGTAAGTGG TGGATCAGAT 
     6841 ATCATCATTG TTGGCAGAGG ACTTTTCGCC AAGGGTAGAG ATCCTAAGGT TGAAGGTGAA 
     6901 AGATACAGAA ATGCTGGATG GGAAGCGTAC CAAAAGAGAA TCAGCGCTCC CCATTAATTA 
     6961 TACAGGAAAC TTAATAGAAC AAATCACATA TTTAATCTAA TAGCCACCTG CATTGGCACG 
     7021 GTGCAACACT CACTTCAACT TCATCTTACA AAAGATCACG TGATCTGTTG TATTGGGATC 
     7081 TCTAG 
//