LOCUS       pILGFP1F5-YFAST2A       6101 bp ds-DNA     circular     16-APR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3260..3319
                     /label="ERBV-1 2A sequence"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3320..4030
                     /label="yEGFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4346..4546
                     /label="URA3(705, 905)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1267..2070
                     /label="KlURA3"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    join(5736..6101,1..495)
                     /label="bla"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2299..2884
                     /label="TEF1P"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      4040..4339
                     /label="PGK1 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4962..5576)
                     /label="pUC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(join(5736..6101,1..495))
                     /label="bla"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        1101..1266
                     /label="URA3p"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2885..3259
                     /label="YFAST"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      4962..5581
                     /label="rep"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
       61 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
      121 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
      181 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
      241 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
      301 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
      361 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
      421 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
      481 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
      541 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
      601 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC
      661 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA
      721 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
      781 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA
      841 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA
      901 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG
      961 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG
     1021 TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
     1081 GACGGCCAGT GAATTCATTT AAATTTTTTT GATTCGGTAA TCTCCGAGCA GAAGGAAGAA
     1141 CGAAGGAAGG AGCACAGACT TAGATTGGTA TATATACGCA TATGTGGTGT TGAAGAAACA
     1201 TGAAATTGCC CAGTATTCTT AACCCAACTG CACAGAACAA AAACCTGCAG GAAACGAAGA
     1261 TAAAACATGT CCACAAAATC ATATACCAGT AGAGCTGAGA CTCATGCAAG TCCGGTTGCA
     1321 TCGAAACTTT TACGTTTAAT GGATGAAAAG AAGACCAATT TGTGTGCTTC TCTTGACGTT
     1381 CGTTCGACTG ATGAGCTATT GAAACTTGTT GAAACGTTGG GTCCATACAT TTGCCTTTTG
     1441 AAAACACACG TTGATATCTT GGATGATTTC AGTTATGAGG GTACTGTCGT TCCATTGAAA
     1501 GCATTGGCAG AGAAATACAA GTTCTTGATA TTTGAGGACA GAAAATTCGC CGATATCGGT
     1561 AACACAGTCA AATTACAATA TACATCGGGC GTTTACCGTA TCGCAGAATG GTCTGATATC
     1621 ACCAACGCCC ACGGGGTTAC TGGTGCTGGT ATTGTTGCTG GCTTGAAACA AGGTGCGCAA
     1681 GAGGTCACCA AAGAACCAAG GGGATTATTG ATGCTTGCTG AATTGTCTTC CAAGGGTTCT
     1741 CTAGCACACG GTGAATATAC TAAGGGTACC GTTGATATTG CAAAGAGTGA TAAAGATTTC
     1801 GTTATTGGGT TCATTGCTCA GAACGATATG GGAGGAAGAG AAGAAGGGTT TGATTGGCTA
     1861 ATCATGACCC CAGGTGTAGG TTTAGACGAC AAAGGCGATG CATTGGGTCA GCAGTACAGA
     1921 ACCGTCGACG AAGTTGTAAG TGGTGGATCA GATATCATCA TTGTTGGCAG AGGACTTTTC
     1981 GCCAAGGGTA GAGATCCTAA GGTTGAAGGT GAAAGATACA GAAATGCTGG ATGGGAAGCG
     2041 TACCAAAAGA GAATCAGCGC TCCCCATTAA TTATACAGGA AACTTAATAG AACAAATCAC
     2101 ATATTTAATC TAATAGCCAC CTGCATTGGC ACGGTGCAAC ACTCACTTCA ACTTCATCTT
     2161 ACAAAAGATC ACGTGATCTG TTGTATTGGG ATCTCTAGAC CTAATAACTT CGTATAGCAT
     2221 ACATTATACG AAGTTATATT AAGGGTTGCT CGAGAAAGAG CTCCCAGAAA GCGACCACCC
     2281 AACTTTGGCT GATAATAGCG TATAAACAAT GCATACTTTG TACGTTCAAA ATACAATGCA
     2341 GTAGATATAT TTATGCATAT TACATATAAT ACATATCACA TAGGAAGCAA CAGGCGCGTT
     2401 GGACTTTTAA TTTTCGAGGA CCGCGAATCC TTACATCACA CCCAATCCCC CACAAGTGAT
     2461 CCCCCACACA CCATAGCTTC AAAATGTTTC TACTCCTTTT TTACTCTTCC AGATTTTCTC
     2521 GGACTCCGCG CATCGCCGTA CCACTTCAAA ACACCCAAGC ACAGCATACT AAATTTCCCC
     2581 TCTTTCTTCC TCTAGGGTGT CGTTAATTAC CCGTACTAAA GGTTTGGAAA AGAAAAAAGA
     2641 GACCGCCTCG TTTCTTTTTC TTCGTCGAAA AAGGCAATAA AAATTTTTAT CACGTTTCTT
     2701 TTTCTTGAAA ATTTTTTTTT TTGATTTTTT TCTCTTTCGA TGACCTCCCA TTGATATTTA
     2761 AGTTAATAAA CGGTCTTCAA TTTCTCAAGT TTCAGTTTCA TTTTTCTTGT TCTATTACAA
     2821 CTTTTTTTAC TTCTTGCTCA TTAGAAAGAA AGCATAGCAA TCTAATCTAA GTTTTAATTA
     2881 CAAAATGGAA CACGTTGCTT TCGGTTCTGA AGATATTGAA AACACTTTGG CTAAAATGGA
     2941 TGATGGTCAA TTAGACGGTT TAGCTTTCGG TGCTATTCAA TTGGACGGTG ATGGTAACAT
     3001 TTTGCAATAC AATGCTGCTG AAGGTGATAT TACTGGTAGA GATCCAAAAC AAGTTATTGG
     3061 TAAGAACTTC TTTAAGGACG TTGCTCCAGG TACTGACTCT CCAGAGTTCT ATGGTAAGTT
     3121 TAAGGAAGGT GTTGCTTCTG GTAACTTAAA CACTATGTTT GAATGGATGA TCCCAACTTC
     3181 CAGAGGTCCA ACTAAGGTTA AAGTTCACAT GAAAAAGGCT TTATCTGGTG ACTCTTACTG
     3241 GGTTTTCGTT AAAAGAGTTG GTGCTACTAA CTTCTCTTTG TTGAAGTTGG CTGGTGACGT
     3301 TGAACTTAAC CCAGGTCCAT CTAAAGGTGA AGAATTATTC ACTGGTGTTG TCCCAATTTT
     3361 GGTTGAATTA GATGGTGATG TTAATGGTCA CAAATTTTCT GTCTCCGGTG AAGGTGAAGG
     3421 TGATGCTACT TACGGTAAAT TGACCTTAAA ATTTATTTGT ACTACTGGTA AATTGCCAGT
     3481 TCCATGGCCA ACCTTAGTCA CTACTTTCGG TTATGGTGTT CAATGTTTTG CGAGATACCC
     3541 AGATCATATG AAACAACATG ACTTTTTCAA GTCTGCCATG CCAGAAGGTT ATGTTCAAGA
     3601 AAGAACTATT TTTTTCAAAG ATGACGGTAA CTACAAGACC AGAGCTGAAG TCAAGTTTGA
     3661 AGGTGATACC TTAGTTAATA GAATCGAATT AAAAGGTATT GATTTTAAAG AAGATGGTAA
     3721 CATTTTAGGT CACAAATTGG AATACAACTA TAACTCTCAC AATGTTTACA TCATGGCTGA
     3781 CAAACAAAAG AATGGTATCA AAGTTAACTT CAAAATTAGA CACAACATTG AAGATGGTTC
     3841 TGTTCAATTA GCTGACCATT ATCAACAAAA TACTCCAATT GGTGATGGTC CAGTCTTGTT
     3901 ACCAGACAAC CATTACTTAT CCACTCAATC TGCCTTATCC AAAGATCCAA ACGAAAAGAG
     3961 AGACCACATG GTCTTGTTAG AATTTGTTAC TGCTGCTGGT ATTACCCATG GTATGGATGA
     4021 ATTGTACAAA AGATCTTAAA TTGAATTGAA TTGAAATCGA TAGATCAATT TTTTTCTTTT
     4081 CTCTTTCCCC ATCCTTTACG CTAAAATAAT AGTTTATTTT ATTTTTTGAA TATTTTTTAT
     4141 TTATATACGT ATATATAGAC TATTATTTAT CTTTTAATGA TTATTAAGAT TTTTATTAAA
     4201 AAAAAATTCG CTCCTCTTTT AATGCCTTTA TGCAGTTTTT TTTTCCCATT CGATATTTCT
     4261 ATGTTCGGGT TCAGCGTATT TTAAGTTTAA TAACTCGAAA ATTCTGCGTT CGTTAAAGCT
     4321 TTCGAGAAGG ATATTATTTA CTAGTAGGAC TATTTGCAAA GGGAAGGGAT GCTAAGGTAG
     4381 AGGGTGAACG TTACAGAAAA GCAGGCTGGG AAGCATATTT GAGAAGATGC GGCCAGCAAA
     4441 ACTAAAAAAC TGTATTATAA GTAAATGCAT GTATACTAAA CTCACAAATT AGAGCTTCAA
     4501 TTTAATTATA TCAGTTATTA CCCGGGAATC TCGGTCGTAA TGATTTAAAT GCATGCAAGC
     4561 TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA
     4621 CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA
     4681 CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG
     4741 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
     4801 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
     4861 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
     4921 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
     4981 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
     5041 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
     5101 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
     5161 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
     5221 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
     5281 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
     5341 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
     5401 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
     5461 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
     5521 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC
     5581 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     5641 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     5701 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     5761 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     5821 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC
     5881 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     5941 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     6001 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     6061 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT C
//