LOCUS       Exported               12644 bp ds-DNA     circular SYN 15-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12644)
  AUTHORS   YLZ
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 4, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12644
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3416..3448
                     /codon_start=1
                     /label=NES
                     /translation="LYPERLRRILT"
     CDS             3455..6974
                     /codon_start=1
                     /label=PguCas13b
                     /translation="TEQSERPYNGTYYTLEDKHFWAAFLNLARHNAYITLTHIDRQLAY
                     SKADITNDQDVLSFKALWKNFDNDLERKSRLRSLILKHFSFLEGAAYGKKLFESKSSGN
                     KSSKNKELTKKEKEELQANALSLDNLKSILFDFLQKLKDFRNYYSHYRHSGSSELPLFD
                     GNMLQRLYNVFDVSVQRVKIDHEHNDEVDPHYHFNHLVRKGKKDRYGHNDNPSFKHHFV
                     DGEGMVTEAGLLFFVSLFLEKRDAIWMQKKIRGFKGGTETYQQMTNEVFCRSRISLPKL
                     KLESLRMDDWMLLDMLNELVRCPKPLYDRLREDDRACFRVPVDILPDEDDTDGGGEDPF
                     KNTLVRHQDRFPYFALRYFDLKKVFTSLRFHIDLGTYHFAIYKKMIGEQPEDRHLTRNL
                     YGFGRIQDFAEEHRPEEWKRLVRDLDYFETGDKPYISQTSPHYHIEKGKIGLRFMPEGQ
                     HLWPSPEVGTTRTGRSKYAQDKRLTAEAFLSVHELMPMMFYYFLLREKYSEEVSAERVQ
                     GRIKRVIEDVYAVYDAFARDEINTRDELDACLADKGIRRGHLPRQMIAILSQEHKDMEE
                     KIRKKLQEMMADTDHRLDMLDRQTDRKIRIGRKNAGLPKSGVIADWLVRDMMRFQPVAK
                     DASGKPLNNSKANSTEYRMLQRALALFGGEKERLTPYFRQMNLTGGNNPHPFLHETRWE
                     SHTNILSFYRSYLRARKAFLERIGRSDRVENRPFLLLKEPKTDRQTLVAGWKGEFHLPR
                     GIFTEAVRDCLIEMGHDEVASYKEVGFMAKAVPLYFERACEDRVQPFYDSPFNVGNSLK
                     PKKGRFLSKEERAEEWERGKERFRDLEAWSYSAARRIEDAFAGIEYASPGNKKKIEQLL
                     RDLSLWEAFESKLKVRADRINLAKLKKEILEAQEHPYHDFKSWQKFERELRLVKNQDII
                     TWMMCRDLMEENKVEGLDTGTLYLKDIRPNVQEQGSLNVLNRVKPMRLPVVVYRADSRG
                     HVHKEEAPLATVYIEERDTKLLKQGNFKSFVKDRRLNGLFSFVDTGGLAMEQYPISKLR
                     VEYELAKYQTARVCVFELTLRLEESLLTRYPHLPDESFREMLESWSDPLLAKWPELHGK
                     VRLLIAVRNAFSHNQYPMYDEAVFSSIRKYDPSSPDAIEERMGLNIAHRLSEEVKQAKE
                     TVERIIQ"
     regulatory      6975..6983
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6980..7698
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             7700..7732
                     /codon_start=1
                     /label=NES
                     /translation="LQLPPLERLTL"
     CDS             7757..7822
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7829..8396
                     /label=IRES
     CDS             8416..9015
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9034..9622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9505..9516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9697..9930
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10135..10239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10240..11100
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11271..11859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12270..12291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12306..12336
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12344..12360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12368..12384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccatgctgta
     3421 ccctgagcgg ctgcggcgga tcctgaccgc tagcacagag cagagcgaga ggccctacaa
     3481 cggcacctac tacaccctgg aagataagca cttctgggcc gcctttctga acctggccag
     3541 acacaacgcc tacatcaccc tgacacacat cgaccggcag ctggcctaca gcaaggccga
     3601 catcaccaac gaccaggacg tgctgagctt caaggccctg tggaagaact tcgacaacga
     3661 cctggaaaga aagagccggc tgcggagcct gatcctgaag cacttcagct ttctggaagg
     3721 cgccgcttac ggcaagaagc tgttcgagtc taagagcagc ggcaacaaga gcagcaagaa
     3781 caaagagctg accaagaaag agaaagagga actgcaggcc aacgctctga gcctggacaa
     3841 cctgaagtct atcctgttcg acttcctgca gaagctgaag gacttccgga actactacag
     3901 ccactaccgg cacagcggaa gcagcgagct gcctctgttc gacggcaaca tgctgcagcg
     3961 gctgtacaac gtgttcgacg tgtccgtgca gcgcgtgaag atcgaccacg agcacaacga
     4021 tgaggtggac cctcactacc acttcaacca cctcgtgcgg aagggcaaga aggacagata
     4081 cggccacaac gacaacccca gcttcaagca ccacttcgtg gacggcgaag gcatggttac
     4141 agaagccggc ctgctgttct tcgtgtccct gttcctggaa aagcgggacg ccatctggat
     4201 gcagaagaag atccggggct tcaaaggcgg caccgagaca taccagcaga tgaccaacga
     4261 ggtgttctgc cggtccagaa tcagcctgcc taagctgaag ctggaaagcc tgcggatgga
     4321 cgactggatg ctgctggaca tgctgaacga actcgtgcgg tgccccaagc ctctgtacga
     4381 cagactgaga gaggacgacc gggcctgctt tagagtgccc gtggatatcc tgcctgacga
     4441 ggacgataca gatggcggcg gagaggaccc cttcaagaat accctcgtcc ggcaccagga
     4501 cagattccct tacttcgccc tgcggtactt cgacctgaag aaggtgttca ccagcctgcg
     4561 gttccacatc gatctgggca cctaccactt tgccatctac aagaagatga tcggcgagca
     4621 gcccgaggac agacacctga ccagaaacct gtacggcttc ggccggatcc aggacttcgc
     4681 cgaagaacac agacccgagg aatggaagcg gctcgtcaga gatctggact acttcgagac
     4741 aggcgacaag ccctacatca gccagacaag cccacactac cacatcgaga agggaaagat
     4801 cggcctgaga ttcatgcccg agggccagca tctttggccc tctccagaag tgggcaccac
     4861 cagaaccggc agatctaagt acgcccagga caagagactg accgccgagg cctttctgtc
     4921 tgtgcacgag ctgatgccta tgatgttcta ctacttcctg ctgcgcgaga agtacagcga
     4981 ggaagtgtct gccgagagag tgcagggcag aatcaagcgc gtgatcgagg atgtgtacgc
     5041 cgtgtacgat gcctttgcca gggacgagat caacacccgg gatgagctgg atgcctgcct
     5101 ggccgataag ggcatcagaa gaggccatct gcctcgccag atgatcgcca tcctgagcca
     5161 agagcacaag gacatggaag agaagattcg gaagaaactg caagagatga tggccgacac
     5221 cgaccaccgg ctggatatgc tggatagaca gaccgaccgg aagatcagaa tcggccggaa
     5281 gaatgccgga ctgcctaaga gcggagtgat cgccgattgg ctcgtgcggg acatgatgag
     5341 atttcagccc gtggccaagg acgccagcgg aaagcctctg aacaactcca aggccaacag
     5401 caccgagtac cggatgctgc agagagccct ggctctgttt ggaggcgaga aagagaggct
     5461 gaccccatac ttccggcaga tgaatctgac cggcggaaac aaccctcatc cttttctgca
     5521 cgaaactcgc tgggagagcc acaccaacat cctgtccttt taccggtcct acctgagagc
     5581 ccgcaaggcc ttcctcgaaa ggatcggcag atccgacaga gtggaaaacc ggccatttct
     5641 gctgctgaaa gagcccaaga ccgacagaca gacactggtg gctggatgga agggcgagtt
     5701 ccatctgcca agaggcatct tcacagaggc cgtgcgggat tgcctgatcg agatgggaca
     5761 cgatgaagtg gccagctaca aagaagtggg attcatggcc aaggccgtgc cactgtactt
     5821 tgagagagcc tgcgaggaca gggtgcagcc cttttacgac agccccttca acgtgggcaa
     5881 cagcctgaag cctaagaagg gcagattcct gagcaaagag gaacgggccg aagagtggga
     5941 gcggggcaaa gagagattcc gggatctcga agcctggtcc tatagcgccg ccagaagaat
     6001 cgaggacgcc tttgccggca tcgagtatgc tagccccgga aacaagaaga aaatcgagca
     6061 gctcctgcgg gacctgagcc tgtgggaagc ctttgagagc aagctgaaag tgcgggccga
     6121 ccggatcaat ctggccaagc tgaagaaaga aatcctggaa gcccaagaac acccctacca
     6181 cgacttcaag agctggcaga agttcgagcg cgagctgcgg ctggtcaaga accaggatat
     6241 catcacctgg atgatgtgcc gcgacctgat ggaagaaaac aaggtggaag gcctggacac
     6301 cggcacactg tacctgaagg acatcagacc caacgtgcaa gagcagggca gcctgaacgt
     6361 gctgaacaga gtgaagccca tgagactgcc cgtggtggtg tacagagccg atagcagagg
     6421 ccacgtgcac aaagaagaag cccctctggc caccgtgtac atcgaggaaa gagacaccaa
     6481 gctgctgaag cagggcaact tcaagtcctt cgtgaaggac aggcggctga acggcctgtt
     6541 ctccttcgtt gatactggcg gactggccat ggaacagtac cccatctcca agctgagagt
     6601 ggaatacgag ctggccaagt accagaccgc cagagtgtgt gtgttcgagc tgaccctgag
     6661 actggaagag tccctgctga cccggtatcc tcatctgccc gacgagagct tcagagagat
     6721 gctggaatct tggagcgacc ctctgctggc caaatggcct gaactgcacg gaaaagtgcg
     6781 gctgctgatc gccgtgcgga atgcctttag ccacaatcag taccctatgt acgacgaggc
     6841 cgtgttcagc tccatccgga agtacgatcc tagcagcccc gacgccatcg aagagagaat
     6901 gggcctgaat atcgcccacc gcctgtctga ggaagtgaag caggccaaag aaaccgtgga
     6961 acggatcatt caggccagta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     7021 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg
     7081 cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     7141 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     7201 ctaccccgac cacatgaagc gccacgactt cttcaagtcc gccatgcccg aaggctacgt
     7261 ccaggagcgc accatcagct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa
     7321 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     7381 cggcaacatc ctggggcaca agctggagta caacttcaac agccacaacg tctatatcac
     7441 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acgtggagga
     7501 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     7561 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     7621 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat
     7681 ggacgagctg tacaagtacc ttcaactgcc tccacttgaa agactgacac tgctagagca
     7741 acgcgtgcag ctaagcgact acaaagacca tgacggtgat tataaagatc atgacatcga
     7801 ttacaaggat gacgatgaca agtaaggatc cctccccccc ccctaacgtt actggccgaa
     7861 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     7921 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     7981 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     8041 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     8101 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     8161 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     8221 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     8281 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     8341 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataaggat
     8401 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8461 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8521 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8581 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8641 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8701 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8761 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8821 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8881 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8941 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     9001 aagcccggtg cctgacgccc atcgatagat cctaatcaac ctctggatta caaaatttgt
     9061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     9121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9421 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9601 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     9661 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9721 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9781 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9841 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9901 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     9961 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
    10021 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
    10081 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
    10141 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10201 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    10261 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    10321 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10381 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10441 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
    10501 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10561 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10621 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10681 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10741 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10801 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10861 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10921 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10981 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    11041 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    11101 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    11161 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    11221 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11281 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    11341 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    11401 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    11461 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    11521 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11581 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11641 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11701 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11821 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11881 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11941 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    12001 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    12061 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    12121 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    12181 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    12241 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    12301 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    12361 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    12421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    12481 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    12541 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    12601 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
