LOCUS       Exported               12404 bp ds-DNA     circular SYN 15-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   YLZ
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 4, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3416..3448
                     /codon_start=1
                     /label=NES
                     /translation="LYPERLRRILT"
     misc_feature    3455..6736
                     /label=RanCas13b
     CDS             6740..7458
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             7460..7492
                     /codon_start=1
                     /label=NES
                     /translation="LQLPPLERLTL"
     CDS             7517..7582
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7589..8156
                     /label=IRES
     CDS             8176..8775
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8794..9382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9265..9276)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9457..9690
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9895..9999
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10000..10860
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11031..11619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12030..12051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12066..12096
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12104..12120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12128..12144
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccatgctgta
     3421 ccctgagcgg ctgcggcgga tcctgaccgc tagcgagaag cccctgctgc ctaacgtgta
     3481 taccctgaag cacaagttct tttggggcgc cttcctgaat atcgcccggc acaacgcctt
     3541 tatcaccatc tgccacatca atgagcagct gggcctgaaa acccccagca acgacgataa
     3601 gatcgtggac gtggtgtgcg agacatggaa caatatcctg aacaatgacc acgatctgct
     3661 gaagaagtcc cagctgaccg agctgatcct gaagcacttc ccttttctga cagccatgtg
     3721 ctaccacccc cctaagaagg agggcaagaa gaagggccac cagaaggagc agcagaagga
     3781 gaaggagagc gaggcacagt cccaggcaga ggccctgaac ccatccaagc tgatcgaggc
     3841 cctggagatc ctggtgaatc agctgcactc tctgcggaac tactatagcc actataagca
     3901 caagaagccc gacgccgaga aggatatctt caagcacctg tataaggcct tcgacgcctc
     3961 tctgagaatg gtgaaggagg attataaggc ccacttcacc gtgaatctga caagggactt
     4021 tgcccacctg aaccgcaagg gcaagaacaa gcaggacaat cccgacttca acagatacag
     4081 attcgagaag gatggcttct ttaccgagtc tggcctgctg ttctttacaa atctgtttct
     4141 ggacaagagg gatgcctatt ggatgctgaa gaaggtgtcc ggcttcaagg cctctcacaa
     4201 gcagcgcgag aagatgacca cagaggtgtt ttgcaggagc cgcatcctgc tgcccaagct
     4261 gaggctggag tcccgctacg accacaacca gatgctgctg gatatgctgt ctgagctgag
     4321 cagatgtcct aagctgctgt atgagaagct gagcgaggag aataagaagc acttccaggt
     4381 ggaggccgac ggctttctgg atgagatcga ggaggagcag aacccattca aggacaccct
     4441 gatccggcac caggatagat tcccctactt tgccctgagg tatctggacc tgaatgagtc
     4501 cttcaagtct atccgctttc aggtggatct gggcacatac cactattgta tctacgacaa
     4561 gaagatcggc gatgagcagg agaagaggca cctgacccgc acactgctgt ccttcggccg
     4621 gctgcaggac tttaccgaga tcaacagacc ccaggagtgg aaggccctga ccaaggacct
     4681 ggattacaag gagacatcca atcagccttt catctctaag accacaccac actatcacat
     4741 caccgacaac aagatcggct ttcggctggg cacaagcaag gagctgtacc cctccctgga
     4801 gatcaaggat ggcgccaata gaatcgccaa gtacccttat aactccggct tcgtggccca
     4861 cgcctttatc tctgtgcacg agctgctgcc tctgatgttc taccagcacc tgaccggcaa
     4921 gagcgaggac ctgctgaagg agacagtgcg gcacatccag agaatctata aggacttcga
     4981 ggaggagcgg atcaatacca tcgaggatct ggagaaggca aaccagggca gactgccact
     5041 gggagccttt cccaagcaga tgctgggcct gctgcagaat aagcagcctg atctgtccga
     5101 gaaggccaag atcaagatcg agaagctgat cgccgagaca aagctgctgt ctcacaggct
     5161 gaacacaaag ctgaagagct ccccaaagct gggcaagcgg agagagaagc tgatcaagac
     5221 aggcgtgctg gccgactggc tggtgaagga tttcatgcgc tttcagcccg tggcctacga
     5281 cgcccagaat cagcctatca agtctagcaa ggccaactcc accgagttct ggtttatcag
     5341 gcgcgccctg gccctgtacg gaggagagaa gaataggctg gagggctatt tcaagcagac
     5401 caacctgatc ggcaacacaa atccacaccc cttcctgaac aagtttaatt ggaaggcctg
     5461 caggaatctg gtggatttct accagcagta tctggagcag cgcgagaagt ttctggaggc
     5521 catcaagaac cagccatggg agccctacca gtattgcctg ctgctgaaga tccctaagga
     5581 gaacaggaag aatctggtga agggatggga gcagggagga atctctctgc cacggggcct
     5641 gtttaccgag gccatcagag agacactgtc tgaggacctg atgctgagca agccaatccg
     5701 caaggagatc aagaagcacg gccgggtggg cttcatcagc agagccatca ccctgtactt
     5761 taaggagaag tatcaggata agcaccagag cttctacaat ctgtcctata agctggaggc
     5821 aaaggcacca ctgctgaagc gggaggagca ctacgagtat tggcagcaga acaagcctca
     5881 gtctccaacc gagagccaga ggctggagct gcacacaagc gaccgctgga aggattacct
     5941 gctgtataag agatggcagc acctggagaa gaagctgcgg ctgtaccgga atcaggacgt
     6001 gatgctgtgg ctgatgaccc tggagctgac aaagaaccac ttcaaggagc tgaacctgaa
     6061 ttatcaccag ctgaagctgg agaatctggc cgtgaacgtg caggaggccg atgccaagct
     6121 gaaccctctg aatcagaccc tgcccatggt gctgcctgtg aaggtgtacc ctgccacagc
     6181 ctttggcgag gtgcagtacc acaagacccc aatcaggaca gtgtatatcc gcgaggagca
     6241 caccaaggcc ctgaagatgg gcaacttcaa ggccctggtg aaggaccgga gactgaatgg
     6301 cctgttctct tttatcaagg aggagaacga tacacagaag cacccaatca gccagctgcg
     6361 gctgaggcgc gagctggaga tctaccagtc tctgagagtg gacgccttca aggagacact
     6421 gagcctggag gagaagctgc tgaataagca cacatccctg tcctctctgg agaacgagtt
     6481 tagggccctg ctggaggagt ggaagaagga gtatgccgcc agctccatgg tgacagacga
     6541 gcacatcgcc ttcatcgcca gcgtgcgcaa tgccttctgc cacaaccagt accccttcta
     6601 caaggaggcc ctgcacgccc ctatcccact gttcaccgtg gcccagccca ccacagagga
     6661 gaaggatggc ctgggaatcg cagaggccct gctgaaggtg ctgagggagt actgtgagat
     6721 cgtgaagtcc cagatcagta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     6781 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg
     6841 cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     6901 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     6961 ctaccccgac cacatgaagc gccacgactt cttcaagtcc gccatgcccg aaggctacgt
     7021 ccaggagcgc accatcagct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa
     7081 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     7141 cggcaacatc ctggggcaca agctggagta caacttcaac agccacaacg tctatatcac
     7201 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acgtggagga
     7261 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     7321 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     7381 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat
     7441 ggacgagctg tacaagtacc ttcaactgcc tccacttgaa agactgacac tgctagagca
     7501 acgcgtgcag ctaagcgact acaaagacca tgacggtgat tataaagatc atgacatcga
     7561 ttacaaggat gacgatgaca agtaaggatc cctccccccc ccctaacgtt actggccgaa
     7621 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     7681 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     7741 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     7801 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     7861 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     7921 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     7981 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     8041 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     8101 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataaggat
     8161 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8221 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8281 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8341 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8401 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8461 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8521 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8581 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8641 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8701 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8761 aagcccggtg cctgacgccc atcgatagat cctaatcaac ctctggatta caaaatttgt
     8821 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8881 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8941 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9001 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9061 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9121 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9181 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9241 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9301 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9361 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     9421 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9481 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9541 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9601 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9661 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     9721 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     9781 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9841 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9901 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9961 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    10021 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    10081 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10141 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10201 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
    10261 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10321 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10381 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10441 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10501 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10561 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10621 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10681 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10741 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10801 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10861 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10921 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10981 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11041 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    11101 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    11161 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    11221 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    11281 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11341 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11401 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11461 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11521 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11581 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11641 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11701 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11761 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11821 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    11881 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    11941 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    12001 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    12061 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    12121 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    12181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    12241 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    12301 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    12361 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
