LOCUS       Exported               11984 bp ds-DNA     circular SYN 15-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   YLZ
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 4, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3416..3448
                     /codon_start=1
                     /label=NES
                     /translation="LYPERLRRILT"
     CDS             3455..6313
                     /codon_start=1
                     /label=ESCasR
                     /translation="GKKIHARDLREQRKTDRTEKFADQNKKREAERAVPKKDAAVSVKS
                     VSSVSSKKDNVTKSMAKAAGVKSVFAVGNTVYMTSFGRGNDAVLEQKIVDTSHEPLNID
                     DPAYQLNVVTMNGYSVTGHRGETVSAVTDNPLRRFNGRKKDEPEQSVPTDMLCLKPTLE
                     KKFFGKEFDDNIHIQLIYNILDIEKILAVYSTNAIYALNNMSADENIENSDFFMKRTTD
                     ETFDDFEKKKESTNSREKADFDAFEKFIGNYRLAYFADAFYVNKKNPKGKAKNVLREDK
                     ELYSVLTLIGKLRHWCVHSEEGRAEFWLYKLDELKDDFKNVLDVVYNRPVEEINNRFIE
                     NNKVNIQILGSVYKNTDIAELVRSYYEFLITKKYKNMGFSIKKLRESMLEGKGYADKEY
                     DSVRNKLYQMTDFILYTGYINEDSDRADDLVNTLRSSLKEDDKTTVYCKEADYLWKKYR
                     ESIREVADALDGDNIKKLSKSNIEIQEDKLRKCFISYADSVSEFTKLIYLLTRFLSGKE
                     INDLVTTLINKFDNIRSFLEIMDELGLDRTFTAEYSFFEGSTKYLAELVELNSFVKSCS
                     FDINAKRTMYRDALDILGIESDKTEEDIEKMIDNILQIDANGDKKLKKNNGLRNFIASN
                     VIDSNRFKYLVRYGNPKKIRETAKCKPAVRFVLNEIPDAQIERYYEACCPKNTALCSAN
                     KRREKLADMIAEIKFENFSDAGNYQKANVTSRTSEAEIKRKNQAIIRLYLTVMYIMLKN
                     LVNVNARYVIAFHCVERDTKLYAESGLEVGNIEKNKTNLTMAVMGVKLENGIIKTEFDK
                     SFAENAANRYLRNARWYKLILDNLKKSERAVVNEFRNTVCHLNAIRNININIKEIKEVE
                     NYFALYHYLIQKHLENRFADKKVERDTGDFISKLEEHKTYCKDFVKAYCTPFGYNLVRY
                     KNLTIDGLFDKNYPGKDDSDEQK"
     misc_feature    4334..4351
                     /label=HEPN
     misc_feature    5996..6013
                     /label=HEPN
     CDS             6322..7036
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      6322..6323
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7040..7072
                     /codon_start=1
                     /label=NES
                     /translation="LQLPPLERLTL"
     CDS             7097..7162
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7169..7736
                     /label=IRES
     CDS             7756..8355
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8374..8962
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8845..8856)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9037..9270
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9475..9579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9580..10440
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10611..11199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11610..11631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11646..11676
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11684..11700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11708..11724
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccatgctgta
     3421 ccctgagcgg ctgcggcgga tcctgaccgc tagcggcaag aagatccacg cccgggacct
     3481 gagagagcag aggaagaccg accggacaga gaagttcgcc gatcagaaca agaagaggga
     3541 ggcagagagg gcagtgccca agaaggacgc cgccgtgagc gtgaagtccg tgagctccgt
     3601 gtctagcaag aaggataatg tgaccaagtc tatggccaag gcagcaggag tgaagagcgt
     3661 gttcgcagtg ggcaacaccg tgtacatgac atcctttggc agaggcaatg acgccgtgct
     3721 ggagcagaag atcgtggata catctcacga gccactgaac atcgacgatc ccgcctacca
     3781 gctgaatgtg gtgaccatga acggctattc cgtgacagga cacaggggag agaccgtgtc
     3841 tgccgtgaca gacaacccac tgcggagatt caatggccgc aagaaggacg agcccgagca
     3901 gtctgtgcct accgatatgc tgtgcctgaa gcccacactg gagaagaagt tctttggcaa
     3961 ggagtttgac gataacatcc acatccagct gatctacaat atcctggaca tcgagaagat
     4021 cctggccgtg tacagcacca acgccatcta tgccctgaac aatatgagcg ccgacgagaa
     4081 tatcgagaac tccgatttct ttatgaagag gaccacagat gagaccttcg acgattttga
     4141 gaagaagaag gagtctacaa acagccggga gaaggccgac ttcgatgcct ttgagaagtt
     4201 catcggcaat tacagactgg cctattttgc cgacgccttc tacgtgaata agaagaaccc
     4261 caagggcaag gccaagaatg tgctgcgcga ggataaggag ctgtattctg tgctgaccct
     4321 gatcggcaag ctgaggcact ggtgcgtgca cagcgaggag ggaagagcag agttctggct
     4381 gtacaagctg gacgagctga aggacgactt caagaacgtg ctggatgtgg tgtataatag
     4441 gcctgtggag gagatcaaca atcgcttcat cgagaacaat aaggtgaaca tccagatcct
     4501 gggcagcgtg tacaagaata ccgacatcgc cgagctggtg agatcctact atgagttcct
     4561 gatcacaaag aagtataaga acatgggctt ttccatcaag aagctgcggg agtctatgct
     4621 ggagggcaag ggctacgccg acaaggagta tgattctgtg agaaataagc tgtaccagat
     4681 gaccgacttc atcctgtaca caggctatat caacgaggac agcgataggg ccgacgatct
     4741 ggtgaatacc ctgcgctcct ctctgaagga ggacgataag accacagtgt attgcaagga
     4801 ggccgactac ctgtggaaga agtatcggga gtccatcaga gaggtggccg atgccctgga
     4861 cggcgataac atcaagaagc tgagcaagtc caatatcgag atccaggagg acaagctgag
     4921 gaagtgtttt atcagctacg ccgattctgt gagcgagttc accaagctga tctatctgct
     4981 gacacgcttt ctgtccggca aggagatcaa cgacctggtg accacactga tcaataagtt
     5041 tgataacatc aggtctttcc tggagatcat ggacgagctg ggcctggatc gcaccttcac
     5101 agccgagtac agcttctttg agggctccac caagtatctg gccgagctgg tggagctgaa
     5161 cagctttgtg aagtcctgct ctttcgacat caatgccaag aggaccatgt accgcgacgc
     5221 cctggatatc ctgggcatcg agtccgacaa gacagaggag gatatcgaga agatgatcga
     5281 caacatcctg cagatcgacg ccaatggcga taagaagctg aagaagaaca atggcctgcg
     5341 gaatttcatc gcctctaacg tgatcgatag caatcggttt aagtacctgg tgagatatgg
     5401 caaccctaag aagatcaggg agaccgccaa gtgcaagcct gccgtgcgct tcgtgctgaa
     5461 tgagatccca gatgcccaga tcgagcggta ctatgaggcc tgctgtccaa agaacaccgc
     5521 cctgtgcagc gccaataaga ggcgcgagaa gctggccgac atgatcgccg agatcaagtt
     5581 tgagaacttc tccgatgccg gcaactacca gaaggccaat gtgacctcca gaacatctga
     5641 ggccgagatc aagaggaaga accaggccat catccgcctg tacctgaccg tgatgtatat
     5701 catgctgaag aatctggtga acgtgaatgc cagatacgtg atcgcctttc actgcgtgga
     5761 gagagacaca aagctgtatg ccgagagcgg cctggaggtc ggcaacatcg agaagaataa
     5821 gaccaacctg acaatggccg tgatgggcgt gaagctggag aatggcatca tcaagaccga
     5881 gtttgacaag tccttcgccg agaatgccgc caaccgctac ctgcggaacg ccagatggta
     5941 taagctgatc ctggataatc tgaagaagtc tgagcgggcc gtggtgaatg agttcagaaa
     6001 caccgtgtgc cacctgaacg ccatcaggaa catcaatatc aacatcaagg agatcaagga
     6061 ggtggagaac tactttgccc tgtaccacta tctgatccag aagcacctgg agaatcggtt
     6121 cgccgacaag aaggtggaga gagacaccgg cgacttcatc tccaagctgg aggagcacaa
     6181 gacctactgc aaggactttg tgaaggccta ttgtacaccc ttcggctaca acctggtgcg
     6241 ctataagaat ctgacaatcg acggcctgtt cgataagaac taccctggca aggacgatag
     6301 cgacgagcag aagagtctag aggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     6361 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg
     6421 cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     6481 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     6541 ctaccccgac cacatgaagc gccacgactt cttcaagtcc gccatgcccg aaggctacgt
     6601 ccaggagcgc accatcagct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa
     6661 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     6721 cggcaacatc ctggggcaca agctggagta caacttcaac agccacaacg tctatatcac
     6781 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acgtggagga
     6841 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     6901 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     6961 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat
     7021 ggacgagctg tacaagtacc ttcaactgcc tccacttgaa agactgacac tgctagagca
     7081 acgcgtgcag ctaagcgact acaaagacca tgacggtgat tataaagatc atgacatcga
     7141 ttacaaggat gacgatgaca agtaaggatc cctccccccc ccctaacgtt actggccgaa
     7201 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     7261 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     7321 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     7381 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     7441 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     7501 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     7561 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     7621 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     7681 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataaggat
     7741 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     7801 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     7861 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     7921 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     7981 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8041 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8101 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8161 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8221 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8281 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8341 aagcccggtg cctgacgccc atcgatagat cctaatcaac ctctggatta caaaatttgt
     8401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8521 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8581 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8641 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8701 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8761 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8821 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8881 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8941 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     9001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9061 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9121 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9181 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9241 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     9301 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     9361 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9421 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9541 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9601 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9661 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9721 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9781 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     9841 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9901 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9961 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10021 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10081 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10141 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10201 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10261 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10321 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10381 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10441 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10501 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10561 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10741 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10981 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11401 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    11461 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    11521 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    11581 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    11641 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    11701 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    11761 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11821 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11881 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    11941 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
