LOCUS       Exported               11960 bp ds-DNA     circular SYN 15-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   YLZ
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 4, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3416..3448
                     /codon_start=1
                     /label=NES
                     /translation="LYPERLRRILT"
     CDS             3455..6289
                     /codon_start=1
                     /label=AdmCasR
                     /translation="NNKRKTKAKAAGLKSVFFDQKQAVLTTFAKGNNSQIEKKVVNSEV
                     KDLRQPPAFDLELKEKTFYISGKNNINTSRENPLASASLPLSKRQRIRAERIKRAREEN
                     RPYHNVKRVGEDDLRAKADLEKHYFGKEYSDNLKIQIIYNILDINKIISPYINDIVYSM
                     NNLARNDEYIDGKIDVIGSLSSTTDYSSFMSPNKDLEKEKKFSFHRENYKKFVEASKPY
                     MRYYGKVFIRDVKKSKLSTGKGEKIEVMYRSDEEIFTIFQILSYVRQSIMHNDIGNKSS
                     ILAIEKYPARFVGFLSDLLKTKTNDVNRMFIDNNSQTNFWVLFSIFGLQDHTSGADKIC
                     RNFYDFVIKADSKNLGFSLKKIRELMLDLPNANMLRDHQFDTVRSKFYTLLDFIIYQHY
                     LEEKSRIDNMVEKLRMTLKEEEKEVLYAAEAKIVWNAIGAKVINKLVPMMNGDALKEIK
                     RKNRDRKLPQSVIATVQVNSDANVFSGLIYFLTLFLDGKEINEMVSNLITKFENIDSLL
                     HVDREIYKSDEKDLDLEIEKLALFFKGVVRPNAKTDTGAGEISKSFSIFQSAERIIEEL
                     KFIKNVTRMDNEIFPSEGVFLDAANVLGVRGDDFDFSNEFVGDDLHSDANKKIINKING
                     TKEDRNLRNFIINNVVKSRRFQYIARHMNTHYVKQLANNETLNRFVLNKMGDAKIINRY
                     YESISGNTPNIEVRSQIDYLVKRLRSFSFEDLNDVKQKVRPGTNESIEKEKKKALVGLC
                     LTIQYLVYKNLVNINARYTTAFYCLERDSKLKGFGVDVWRDFESYTALTNHFIKEGYLP
                     VRKAEILRANLKHLDCEDGFKYYRNQVTHLNAIRVAYKYINEIKSVHSYFALYHYIMQR
                     HLYDSLQAKAKDSSGFVIDALKKSFEHKIYSKDLLHVLHSPFGYNTARYKNLSIEALFD
                     KNESRPEVNPLSTND"
     misc_feature    4259..4270
                     /label=HEPN
     misc_feature    5963..5977
                     /label=HEPN
     CDS             6298..7012
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      6298..6299
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7016..7048
                     /codon_start=1
                     /label=NES
                     /translation="LQLPPLERLTL"
     CDS             7073..7138
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7145..7712
                     /label=IRES
     CDS             7732..8331
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8350..8938
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8821..8832)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9013..9246
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9451..9555
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9556..10416
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10587..11175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11586..11607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11622..11652
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11660..11676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11684..11700
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccatgctgta
     3421 ccctgagcgg ctgcggcgga tcctgaccgc tagcaacaat aagaggaaga ccaaggcaaa
     3481 ggcagcaggc ctgaagagcg tgttctttga ccagaagcag gccgtgctga ccacattcgc
     3541 caagggcaac aatagccaga tcgagaagaa ggtggtgaac tccgaggtga aggatctgag
     3601 acagccccct gccttcgacc tggagctgaa ggagaagacc ttttacatct ctggcaagaa
     3661 caatatcaac acaagcaggg agaatcctct ggcctctgcc agcctgccac tgagcaagag
     3721 gcagcgcatc cgggcagagc gcatcaagag agccagggag gagaaccgcc cataccacaa
     3781 tgtgaagcgg gtgggcgagg acgatctgag agccaaggcc gatctggaga agcactactt
     3841 cggcaaggag tattccgaca acctgaagat ccagatcatc tataacatcc tggatatcaa
     3901 taagatcatc tctccctaca tcaacgacat cgtgtatagc atgaacaatc tggccaggaa
     3961 tgacgagtac atcgatggca agatcgacgt gatcggcagc ctgagctcca ccacagatta
     4021 ttctagcttt atgtccccaa acaaggacct ggagaaggag aagaagttct cttttcaccg
     4081 cgagaattac aagaagttcg tggaggcctc caagccctat atgaggtact atggcaaggt
     4141 gtttatccgc gatgtgaaga agtctaagct gagcaccggc aagggcgaga agatcgaagt
     4201 gatgtaccgg tctgacgagg agatcttcac aatctttcag atcctgagct atgtgagaca
     4261 gtccatcatg cacaacgata tcggcaataa gtcctctatc ctggccatcg agaagtaccc
     4321 tgcccggttc gtgggctttc tgagcgacct gctgaagacc aagacaaacg atgtgaatag
     4381 aatgttcatc gacaacaatt cccagaccaa cttttgggtg ctgttctcta tctttggcct
     4441 gcaggatcac acaagcggcg ccgacaagat ctgccggaac ttctacgatt ttgtgatcaa
     4501 ggccgactct aagaatctgg gcttcagcct gaagaagatc agagagctga tgctggatct
     4561 gccaaacgcc aatatgctgc gggatcacca gtttgacacc gtgagatcca agttctacac
     4621 actgctggac ttcatcatct accagcacta tctggaggag aagtctagga tcgacaatat
     4681 ggtggagaag ctgcgcatga ccctgaagga ggaggagaag gaggtgctgt atgccgccga
     4741 ggccaagatc gtgtggaacg ccatcggcgc caaagtgatc aacaagctgg tgcccatgat
     4801 gaatggcgat gccctgaagg agatcaagcg gaagaatcgc gaccggaagc tgcctcagtc
     4861 cgtgatcgcc acagtgcagg tgaactccga tgccaacgtg ttcagcggcc tgatctactt
     4921 cctgaccctg tttctggacg gcaaggagat caacgagatg gtgagcaatc tgatcacaaa
     4981 gtttgagaac atcgattccc tgctgcacgt ggacagggag atctataagt ctgacgagaa
     5041 ggacctggat ctggagatcg agaagctggc cctgttcttt aagggcgtgg tgcgccccaa
     5101 tgccaagacc gatacaggcg ccggcgagat ctccaagtct ttcagcatct ttcagagcgc
     5161 cgagcggatc atcgaggagc tgaagttcat caagaacgtg accagaatgg ataatgagat
     5221 cttcccttcc gagggcgtgt ttctggacgc agcaaacgtg ctgggcgtga ggggcgacga
     5281 tttcgatttt tccaacgagt ttgtgggcga cgatctgcac tctgacgcca ataagaagat
     5341 catcaacaag atcaatggca caaaggagga ccgcaacctg cggaatttca tcatcaacaa
     5401 tgtggtgaag agccggagat ttcagtacat cgcccgccac atgaacaccc actatgtgaa
     5461 gcagctggcc aacaatgaga cactgaacag gttcgtgctg aataagatgg gcgacgccaa
     5521 gatcatcaat cgctactatg agtccatctc tggcaacacc cctaatatcg aggtgcggtc
     5581 tcagatcgat tacctggtga agagactgag gagcttctcc tttgaggacc tgaacgatgt
     5641 gaagcagaag gtgagaccag gcaccaatga gagcatcgag aaggagaaga agaaggccct
     5701 ggtgggcctg tgcctgacaa tccagtacct ggtgtataag aacctggtga acatcaatgc
     5761 ccggtacacc acagccttct attgtctgga gagagactct aagctgaagg gcttcggcgt
     5821 ggacgtgtgg cgggactttg agagctacac cgccctgaca aaccacttca tcaaggaggg
     5881 ctatctgccc gtgagaaagg ccgagatcct gagggccaat ctgaagcacc tggactgtga
     5941 ggatggcttt aagtactata ggaaccaggt gacccacctg aatgccatcc gcgtggccta
     6001 caagtatatc aacgagatca agtccgtgca ctcttacttc gccctgtacc actatatcat
     6061 gcagagacac ctgtatgact ccctgcaggc caaggccaag gatagctccg gcttcgtgat
     6121 cgacgccctg aagaagagct ttgagcacaa gatctactcc aaggatctgc tgcacgtgct
     6181 gcacagccct ttcggctaca acaccgccag gtataagaat ctgtccatcg aggccctgtt
     6241 tgataagaat gagtcccgcc cagaggtgaa ccccctgtct acaaatgaca gtctagaggt
     6301 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     6361 cgtaaacggc cacaagttca gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa
     6421 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     6481 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcgcca
     6541 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa
     6601 ggacgacggc acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     6661 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     6721 ggagtacaac ttcaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     6781 caaggccaac ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc tcgccgacca
     6841 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     6901 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6961 ggagttcgtg accgccgccg ggatcactca cggcatggac gagctgtaca agtaccttca
     7021 actgcctcca cttgaaagac tgacactgct agagcaacgc gtgcagctaa gcgactacaa
     7081 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagta
     7141 aggatccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     7201 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     7261 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     7321 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     7381 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     7441 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     7501 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     7561 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     7621 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     7681 acgtggtttt cctttgaaaa acacgatgat aaggatccac cggagcttac catgaccgag
     7741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     7861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     8041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     8101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     8161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     8221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     8281 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccatcg
     8341 atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8401 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8461 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     8521 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     8581 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     8641 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8701 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     8761 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8821 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8881 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     8941 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9001 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     9061 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9121 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9181 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9241 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     9301 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     9361 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     9421 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9481 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9541 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9601 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9661 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9721 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9781 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     9841 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9901 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9961 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    10021 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    10081 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    10141 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    10201 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10261 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    10321 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    10381 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    10441 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10501 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10561 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10621 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10681 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10741 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10801 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10861 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10921 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10981 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    11041 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    11101 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    11161 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    11221 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    11281 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    11341 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    11401 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    11461 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    11521 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    11581 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    11641 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    11701 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    11761 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    11821 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
    11881 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    11941 tgtactacaa acttagtagt
//
