LOCUS       Exported               12218 bp ds-DNA     circular SYN 15-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12218)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 4, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12218
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     promoter        2242..2453
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     promoter        3193..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3193..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3416..3448
                     /codon_start=1
                     /label=NES
                     /translation="LYPERLRRILT"
     CDS             3455..6352
                     /codon_start=1
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             6361..7075
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      6361..6362
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7079..7111
                     /codon_start=1
                     /label=NES
                     /translation="LQLPPLERLTL"
     CDS             7136..7201
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7208..7775
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7795..8589
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    8608..9196
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9079..9090)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9271..9504
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9709..9813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9814..10674
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10845..11433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11844..11865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11880..11910
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11918..11934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11942..11958
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccatgctgta
     3421 ccctgagcgg ctgcggcgga tcctgaccgc tagcatcgag aagaagaaga gcttcgccaa
     3481 gggcatgggc gtgaagagca ccctggtgtc cggctctaag gtgtacatga ccacatttgc
     3541 cgagggctcc gacgcacggc tggagaagat cgtggagggc gatagcatca gatccgtgaa
     3601 cgagggagag gccttcagcg ccgagatggc cgacaagaat gccggctata agatcggcaa
     3661 cgccaagttt tcccacccaa agggctatgc cgtggtggcc aacaatccac tgtacaccgg
     3721 accagtgcag caggacatgc tgggcctgaa ggagacactg gagaagagat acttcggcga
     3781 gagcgccgac ggcaatgata acatctgcat ccaggtcatc cacaatatcc tggatatcga
     3841 gaagatcctg gccgagtata tcaccaacgc cgcctacgcc gtgaacaata tctccggcct
     3901 ggacaaggat atcatcggct tcggcaagtt ttctaccgtg tacacatatg acgagttcaa
     3961 ggaccccgag caccacaggg ccgcctttaa caataacgac aagctgatca atgccatcaa
     4021 ggcccagtac gacgagttcg ataattttct ggataacccc cggctgggct attttggcca
     4081 ggccttcttt tctaaggagg gcagaaacta tatcatcaat tacggcaacg agtgttacga
     4141 catcctggcc ctgctgagcg gactgaggca ctgggtggtg cacaataacg aggaggagtc
     4201 taggatcagc cgcacctggc tgtataatct ggacaagaat ctggataacg agtacatctc
     4261 cacactgaac tacctgtatg atcggatcac caatgagctg acaaacagct tctccaagaa
     4321 ttctgccgcc aatgtgaact acatcgcaga gaccctgggc atcaacccag cagagttcgc
     4381 agagcagtac ttccggttca gcatcatgaa ggagcagaag aatctgggct ttaacatcac
     4441 aaagctgcgg gaagtgatgc tggacagaaa ggatatgtcc gagatccgga agaaccacaa
     4501 ggtgttcgat tctatcagaa ccaaggtgta tacaatgatg gactttgtga tctaccggta
     4561 ctatatcgag gaggatgcca aggtggccgc cgccaataag agcctgcccg acaacgagaa
     4621 gtctctgagc gagaaggata tcttcgtgat caatctgaga ggctccttta acgacgatca
     4681 gaaggacgcc ctgtactatg atgaggccaa tcggatctgg agaaagctgg agaatatcat
     4741 gcacaacatc aaggagttca ggggcaacaa gacccgcgag tacaagaaga aggacgcacc
     4801 aaggctgcct cgcatcctgc ctgcaggaag ggacgtgagc gccttcagca agctgatgta
     4861 tgccctgaca atgtttctgg acggcaagga gatcaatgat ctgctgacca cactgatcaa
     4921 taagttcgac aacatccagt cctttctgaa agtgatgcct ctgatcggcg tgaacgccaa
     4981 gttcgtggag gagtacgcct tctttaagga cagcgccaag atcgccgatg agctgaggct
     5041 gatcaagtcc tttgccagga tgggagagcc aatcgcagac gcaaggagag ccatgtacat
     5101 cgatgccatc agaatcctgg gcaccaatct gtcttatgat gagctgaagg ccctggccga
     5161 caccttcagc ctggacgaga atggcaacaa gctgaagaag ggcaagcacg gcatgcgcaa
     5221 tttcatcatc aataacgtga tcagcaacaa gaggtttcac tatctgatca gatacggcga
     5281 ccctgcacac ctgcacgaga tcgccaagaa cgaggccgtg gtgaagttcg tgctgggcag
     5341 gatcgccgat atccagaaga agcagggcca gaatggcaag aaccagatcg accgctacta
     5401 tgagacctgc atcggcaagg ataagggcaa gtccgtgtct gagaaggtgg acgccctgac
     5461 caagatcatc acaggcatga attatgacca gttcgataag aagcggagcg tgatcgagga
     5521 caccggaagg gagaacgcag agagagagaa gtttaagaag atcatcagcc tgtatctgac
     5581 agtgatctac cacatcctga agaatatcgt gaatatcaac gccagatacg tgatcggctt
     5641 ccactgcgtg gagcgcgatg cccagctgta taaggagaag ggctacgaca tcaacctgaa
     5701 gaagctggag gagaagggct ttagctccgt gaccaagctg tgcgcaggaa tcgacgagac
     5761 agcacccgac aagcggaagg atgtggagaa ggagatggcc gagagagcca aggagagcat
     5821 cgactccctg gagtctgcca atcctaagct gtacgccaac tatatcaagt actccgatga
     5881 gaagaaggcc gaggagttca cccggcagat caatagagag aaggccaaga ccgccctgaa
     5941 cgcctacctg cggaatacaa agtggaacgt gatcatcagg gaggacctgc tgcgcatcga
     6001 taataagacc tgtacactgt tcaggaacaa ggccgtgcac ctggaggtgg cccgctatgt
     6061 gcacgcctac atcaatgaca tcgccgaggt gaactcctac tttcagctgt accactatat
     6121 catgcagcgg atcatcatga acgagagata tgagaagtct agcggcaagg tgtctgagta
     6181 cttcgacgcc gtgaatgatg agaagaagta taacgataga ctgctgaagc tgctgtgcgt
     6241 gcctttcggc tactgtatcc caaggtttaa gaatctgagc atcgaggccc tgttcgacag
     6301 gaacgaggca gccaagtttg ataaggagaa gaagaaggtg agcggcaact ccagtctaga
     6361 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     6421 cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg
     6481 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     6541 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagcg
     6601 ccacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcagctt
     6661 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     6721 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     6781 gctggagtac aacttcaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
     6841 catcaaggcc aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga
     6901 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     6961 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     7021 gctggagttc gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaagtacct
     7081 tcaactgcct ccacttgaaa gactgacact gctagagcaa cgcgtgcagc taagcgacta
     7141 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
     7201 gtaaggatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     7261 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     7321 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     7381 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     7441 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     7501 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     7561 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     7621 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     7681 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     7741 gggacgtggt tttcctttga aaaacacgat gataaggatc caccggagct taccatgatt
     7801 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7861 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7921 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     7981 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     8041 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     8101 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     8161 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8221 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8281 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     8341 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8401 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8461 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8521 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8581 ttcttctgac gcccatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     8641 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8701 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8761 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8821 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8881 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8941 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     9001 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9061 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9121 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9181 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     9241 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     9301 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9361 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9421 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9481 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     9541 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     9601 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     9661 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9721 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9781 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9841 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9901 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9961 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    10021 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
    10081 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    10141 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    10201 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    10261 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    10321 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    10381 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    10441 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    10501 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10561 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10621 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10681 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10741 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10801 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10861 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10921 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10981 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    11041 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    11101 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11161 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11221 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11281 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11341 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11401 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11461 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11521 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    11581 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    11641 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
    11701 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    11761 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
    11821 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    11881 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11941 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
    12001 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    12061 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    12121 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    12181 aattgtattt gttaaatatg tactacaaac ttagtagt
//
