LOCUS       Exported               12205 bp ds-DNA     circular SYN 28-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   YLZ
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 4, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    3472..6369
                     /label=RfxCas13d
     CDS             7087..7107
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7123..7188
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7195..7762
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7782..8576
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    8595..9183
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9066..9077)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9258..9491
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9696..9800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9801..10661
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10832..11420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11831..11852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11867..11897
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11905..11921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11929..11945
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catcgagaag
     3481 aagaagagct tcgccaaggg catgggcgtg aagagcaccc tggtgtccgg ctctaaggtg
     3541 tacatgacca catttgccga gggctccgac gcacggctgg agaagatcgt ggagggcgat
     3601 agcatcagat ccgtgaacga gggagaggcc ttcagcgccg agatggccga caagaatgcc
     3661 ggctataaga tcggcaacgc caagttttcc cacccaaagg gctatgccgt ggtggccaac
     3721 aatccactgt acaccggacc agtgcagcag gacatgctgg gcctgaagga gacactggag
     3781 aagagatact tcggcgagag cgccgacggc aatgataaca tctgcatcca ggtcatccac
     3841 aatatcctgg atatcgagaa gatcctggcc gagtatatca ccaacgccgc ctacgccgtg
     3901 aacaatatct ccggcctgga caaggatatc atcggcttcg gcaagttttc taccgtgtac
     3961 acatatgacg agttcaagga ccccgagcac cacagggccg cctttaacaa taacgacaag
     4021 ctgatcaatg ccatcaaggc ccagtacgac gagttcgata attttctgga taacccccgg
     4081 ctgggctatt ttggccaggc cttcttttct aaggagggca gaaactatat catcaattac
     4141 ggcaacgagt gttacgacat cctggccctg ctgagcggac tgaggcactg ggtggtgcac
     4201 aataacgagg aggagtctag gatcagccgc acctggctgt ataatctgga caagaatctg
     4261 gataacgagt acatctccac actgaactac ctgtatgatc ggatcaccaa tgagctgaca
     4321 aacagcttct ccaagaattc tgccgccaat gtgaactaca tcgcagagac cctgggcatc
     4381 aacccagcag agttcgcaga gcagtacttc cggttcagca tcatgaagga gcagaagaat
     4441 ctgggcttta acatcacaaa gctgcgggaa gtgatgctgg acagaaagga tatgtccgag
     4501 atccggaaga accacaaggt gttcgattct atcagaacca aggtgtatac aatgatggac
     4561 tttgtgatct accggtacta tatcgaggag gatgccaagg tggccgccgc caataagagc
     4621 ctgcccgaca acgagaagtc tctgagcgag aaggatatct tcgtgatcaa tctgagaggc
     4681 tcctttaacg acgatcagaa ggacgccctg tactatgatg aggccaatcg gatctggaga
     4741 aagctggaga atatcatgca caacatcaag gagttcaggg gcaacaagac ccgcgagtac
     4801 aagaagaagg acgcaccaag gctgcctcgc atcctgcctg caggaaggga cgtgagcgcc
     4861 ttcagcaagc tgatgtatgc cctgacaatg tttctggacg gcaaggagat caatgatctg
     4921 ctgaccacac tgatcaataa gttcgacaac atccagtcct ttctgaaagt gatgcctctg
     4981 atcggcgtga acgccaagtt cgtggaggag tacgccttct ttaaggacag cgccaagatc
     5041 gccgatgagc tgaggctgat caagtccttt gccaggatgg gagagccaat cgcagacgca
     5101 aggagagcca tgtacatcga tgccatcaga atcctgggca ccaatctgtc ttatgatgag
     5161 ctgaaggccc tggccgacac cttcagcctg gacgagaatg gcaacaagct gaagaagggc
     5221 aagcacggca tgcgcaattt catcatcaat aacgtgatca gcaacaagag gtttcactat
     5281 ctgatcagat acggcgaccc tgcacacctg cacgagatcg ccaagaacga ggccgtggtg
     5341 aagttcgtgc tgggcaggat cgccgatatc cagaagaagc agggccagaa tggcaagaac
     5401 cagatcgacc gctactatga gacctgcatc ggcaaggata agggcaagtc cgtgtctgag
     5461 aaggtggacg ccctgaccaa gatcatcaca ggcatgaatt atgaccagtt cgataagaag
     5521 cggagcgtga tcgaggacac cggaagggag aacgcagaga gagagaagtt taagaagatc
     5581 atcagcctgt atctgacagt gatctaccac atcctgaaga atatcgtgaa tatcaacgcc
     5641 agatacgtga tcggcttcca ctgcgtggag cgcgatgccc agctgtataa ggagaagggc
     5701 tacgacatca acctgaagaa gctggaggag aagggcttta gctccgtgac caagctgtgc
     5761 gcaggaatcg acgagacagc acccgacaag cggaaggatg tggagaagga gatggccgag
     5821 agagccaagg agagcatcga ctccctggag tctgccaatc ctaagctgta cgccaactat
     5881 atcaagtact ccgatgagaa gaaggccgag gagttcaccc ggcagatcaa tagagagaag
     5941 gccaagaccg ccctgaacgc ctacctgcgg aatacaaagt ggaacgtgat catcagggag
     6001 gacctgctgc gcatcgataa taagacctgt acactgttca ggaacaaggc cgtgcacctg
     6061 gaggtggccc gctatgtgca cgcctacatc aatgacatcg ccgaggtgaa ctcctacttt
     6121 cagctgtacc actatatcat gcagcggatc atcatgaacg agagatatga gaagtctagc
     6181 ggcaaggtgt ctgagtactt cgacgccgtg aatgatgaga agaagtataa cgatagactg
     6241 ctgaagctgc tgtgcgtgcc tttcggctac tgtatcccaa ggtttaagaa tctgagcatc
     6301 gaggccctgt tcgacaggaa cgaggcagcc aagtttgata aggagaagaa gaaggtgagc
     6361 ggcaactcca gtctagaggt gtccaagggc gaagaggata acatggccat catcaaggag
     6421 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     6481 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     6541 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     6601 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     6661 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     6721 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     6781 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     6841 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     6901 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     6961 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     7021 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     7081 aagtacccca agaaaaagcg caaggtacgc gtgcagctaa gcgactacaa agaccatgac
     7141 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagta aggatccctc
     7201 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     7261 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     7321 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     7381 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     7441 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     7501 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     7561 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     7621 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     7681 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     7741 cctttgaaaa acacgatgat aaggatccac cggagcttac catgattgaa caagatggat
     7801 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7861 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7921 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     7981 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     8041 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     8101 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     8161 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     8221 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     8281 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     8341 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     8401 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     8461 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     8521 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgacgcc
     8581 catcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     8641 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     8701 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     8761 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     8821 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     8881 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8941 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     9001 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     9061 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9121 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9181 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     9241 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     9301 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9481 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     9541 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa
     9601 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     9661 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9781 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9841 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9901 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9961 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10021 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
    10081 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10141 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10201 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10261 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10321 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10381 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10441 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10501 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10561 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10621 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10681 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10741 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    11401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11461 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11521 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11581 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    11641 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
    11701 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    11761 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    11821 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    11881 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    11941 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12001 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    12061 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    12121 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    12181 aaatatgtac tacaaactta gtagt
//
