LOCUS       Exported               10276 bp ds-DNA     circular SYN 23-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Sho Yoshimatsu
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2021 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     439..458
                     /label=f1ori-3
     primer_bind     complement(504..514)
                     /label=NheI-hG-5
     primer_bind     552..571
                     /label=pCR-BEnd-3
     primer_bind     596..616
                     /label=AgeI-M13fwd-5
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     600..616
                     /label=M13 fwd
     primer_bind     600..616
                     /label=pDEST-M13fwd
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     623..641
                     /label=T7-5
     misc_feature    complement(656..968)
                     /gene="PB5'TR"
                     /label=PB5'TR
     primer_bind     656..680
                     /label=XhoI-PBR-R
     primer_bind     complement(785..802)
                     /label=PBR-F
     primer_bind     complement(869..891)
                     /label=PBLm-F2
     primer_bind     complement(941..969)
                     /label=PB6R-Hprt-R
     primer_bind     950..990
                     /label=PB6R-mih-F
     primer_bind     970..1000
                     /label=L1-CMV-5
     primer_bind     1049..1068
                     /label=pPB-CMV-F
     primer_bind     1051..1070
                     /label=CAG-start-5
     enhancer        1052..1355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1052..1067
                     /label=EcoRI-KpnI-CMV-5
     enhancer        1071..1358
                     /label=CAG enhancer
     primer_bind     1141..1163
                     /label=CAG-F1
     primer_bind     1198..1207
                     /label=cjHPRTgg2-F3
     primer_bind     complement(1209..1228)
                     /label=pSpCas9-600-3
     primer_bind     complement(1332..1356)
                     /label=CMVe-R
     primer_bind     complement(1337..1359)
                     /label=pPB-CMV-R
     promoter        1360..1637
                     /label=chicken beta-actin promoter
     primer_bind     1623..1637
                     /label=CBA-F
     intron          1638..2654
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          1639..2609
                     /label=chicken beta-actin intron I
     primer_bind     complement(1650..1661)
                     /label=B2-FOXL2-R
     primer_bind     complement(1694..1704)
                     /label=pE2-cjNANOS3-F
     primer_bind     complement(1768..1790)
                     /label=CAG-R1
     primer_bind     1774..1796
                     /label=CAG-F2
     primer_bind     complement(1865..1877)
                     /label=Ori-ApaLI-3
     primer_bind     1899..1909
                     /label=ApaI-EF-F
     primer_bind     complement(1938..1950)
                     /label=LinkerForHNEO-cjPLP1-bot
     primer_bind     complement(1942..1951)
                     /label=SOX2-gRNA-2R
     primer_bind     1991..2002
                     /label=ApaI-EF-F
     primer_bind     complement(2157..2167)
                     /label=l-E2A-3
     primer_bind     2184..2194
                     /label=cjNANOS3-seq1
     primer_bind     2404..2415
                     /label=LinkerForHNEO-cjPLP1-bot
     primer_bind     complement(2421..2433)
                     /label=cjOCTcis-F
     primer_bind     complement(2424..2434)
                     /label=NotI-BamHI-SacI-3
     primer_bind     complement(2468..2478)
                     /label=Tomato-start-R
     primer_bind     2536..2552
                     /label=cbai-5
     primer_bind     2591..2607
                     /label=CAG-Xba-F
     intron          2610..2654
                     /label=rabbit beta-globin intron II
     primer_bind     2610..2629
                     /label=rbgi-5
     primer_bind     complement(2616..2638)
                     /label=CAG-R2
     primer_bind     2618..2640
                     /label=CAG-OCT4-F1
     primer_bind     2682..2703
                     /label=Xho-Nhe-Not-RfA-F
     primer_bind     2684..2703
                     /label=pCE-CDS-F
     primer_bind     complement(2685..2708)
                     /label=pENTR-R3-CAG-R
     primer_bind     complement(2692..2708)
                     /label=CAG-EcoRI-AnkBAS-3
     primer_bind     complement(2697..2723)
                     /label=CAG-koR
     CDS             2721..3461
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLP"
     primer_bind     2721..2740
                     /label=P2A-rtTA-F
     primer_bind     complement(2872..2890)
                     /label=ApaI-rtTA-R
     primer_bind     3262..3279
                     /label=rtTA-F
     primer_bind     3444..3529
                     /label=rtTA-T2A-SOX17-F
     CDS             3462..3515
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3462..3481)
                     /label=T2A-R (2)
     primer_bind     3462..3479
                     /label=pCXLE-EGFP-T2A-F
     primer_bind     complement(3496..3517)
                     /label=pMaster12-T2A-R
     primer_bind     complement(3497..3515)
                     /label=T2A-R
     primer_bind     complement(3498..3516)
                     /label=pPBCD2-B2R
     primer_bind     3514..3531
                     /label=pCR-SOX17e1-F
     CDS             3516..4757
                     /codon_start=1
                     /label=SOX17
                     /translation="MSSPDAGYASDDQSQTQSALPAVMAGLGPCPWAESLSPIGDMKVK
                     GEAPANSGAPAGAAGRAKGESRIRRPMNAFMVWAKDERKRLAQQNPDLHNAELSKMLGK
                     SWKALTLAEKRPFVEEAERLRVQHMQDHPNYKYRPRRRKQVKRLKRVEGGFLHGLAEPQ
                     AAALGPEGGRVAMDGLGLQFPEQGFPAGPPLLPPHMGGHYRDCQSLGAPPLDGYPLPTP
                     DTSPLDGVDPDPAFFAAPMPGDCPAAGTYSYAQVSDYAGPPEPPAGPMHPRLGPEPAGP
                     SIPGLLAPPSALHVYYGAMGSPGAGGGRGFQMQPQHQHQHQHQHHPPGPGQPSPPPEAL
                     PCRDGTDPSQPAELLGEVDRTEFEQYLHFVCKPEMGLPYQGHDSGVNLPDSHGAISSVV
                     SDASSAVYYCNYPDV"
     primer_bind     3737..3758
                     /label=SOX17-tF
     primer_bind     complement(3803..3842)
                     /label=pCR-SOX17e1-R
     primer_bind     complement(3825..3843)
                     /label=SOX17-tR
     primer_bind     3825..3842
                     /label=B1-SOX17(103)-F
     primer_bind     3903..3924
                     /label=END-F2
     primer_bind     3905..3927
                     /label=END-F1
     primer_bind     complement(3926..3936)
                     /label=ApaI-EF-F
     primer_bind     3998..4008
                     /label=ApaI-EF-F
     primer_bind     4577..4594
                     /label=SOX17-seq1
     primer_bind     complement(4739..4762)
                     /label=B2-SOX2-R
     primer_bind     complement(4739..4757)
                     /label=SOX17-R
     primer_bind     4740..4776
                     /label=SOX17-GR-F
     misc_feature    4758..4766
                     /label=GSG linker
     misc_feature    4767..5600
                     /label=hGR domain
     gene            4767..5600
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /label=NR3C1
                     /note="nuclear receptor subfamily 3 group C member 1"
                     /db_xref="GeneID:2908"
                     /db_xref="HGNC:HGNC:7978"
                     /db_xref="MIM:138040"
     CDS             4767..5600
                     /codon_start=1
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /product="glucocorticoid receptor isoform alpha"
                     /label=NR3C1
                     /note="isoform alpha is encoded by transcript variant 1; 
                     glucocorticoid nuclear receptor variant 1; nuclear receptor
                     subfamily 3, group C, member 1 (glucocorticoid receptor); 
                     nuclear receptor subfamily 3 group C member 1 variant 
                     hGR-B(54); nuclear receptor subfamily 3 group C member 1 
                     variant hGR-B(77); nuclear receptor subfamily 3 group C 
                     member 1 variant hGR-B(93)"
                     /db_xref="CCDS:CCDS4278.1"
                     /db_xref="GeneID:2908"
                     /db_xref="HGNC:HGNC:7978"
                     /db_xref="MIM:138040"
                     /protein_id="NP_000167.1"
                     /translation="IQQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVL
                     YAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFL
                     MAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVSSELHRLQVSYEE
                     YLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKL
                     LDSMHEVVENLLNYCFQTFLDKTMSTEFPEMLAEIITNQIPKYSNGNIKKLLFHQK"
     misc_feature    4767..5600
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /note="propagated from UniProtKB/Swiss-Prot (P04150.1); 
                     Region: Interaction with CLOCK"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            4767..5016
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /inference="alignment:same species:2.1.0"
     misc_feature    4767..4838
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /note="propagated from UniProtKB/Swiss-Prot (P04150.1); 
                     Region: Hinge"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    4863..5360
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /note="propagated from UniProtKB/Swiss-Prot (P04150.1); 
                     Region: Interaction with CRY1. 
                     {ECO:0000269|PubMed:22170608}"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            5017..5161
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /inference="alignment:same species:2.1.0"
     exon            5162..5292
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /inference="alignment:same species:2.1.0"
     exon            5293..5450
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /inference="alignment:same species:2.1.0"
     exon            5451..5600
                     /gene="NR3C1"
                     /gene_synonym="GCCR; GCR; GCRST; GR; GRL"
                     /inference="alignment:same species:2.1.0"
     misc_feature    5508..5510
                     /label=I747T (att -> act)
     CDS             5610..5633
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(5614..5633)
                     /label=Strep-R
     primer_bind     complement(5617..5654)
                     /label=StrepTag-PB-R
     primer_bind     complement(5618..5633)
                     /label=attB2-EncA-link-R
     primer_bind     complement(5620..5636)
                     /label=pENTR-B1-hGR-R
     primer_bind     5639..5656
                     /label=pPB-IRES-F
     primer_bind     complement(5650..5669)
                     /label=IRES-start-3
     primer_bind     5653..5667
                     /label=MfeI-IRES-5
     misc_feature    5654..6228
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     5654..5671
                     /label=EcoRI-MluI-IRES-F
     primer_bind     complement(5750..5769)
                     /label=IRES2+100-3
     primer_bind     5786..5805
                     /label=IRES2-Bln-F
     primer_bind     complement(5811..5829)
                     /label=IRES-BlnI-R
     primer_bind     5931..5942
                     /label=cjHPRTgg2-F3
     primer_bind     5993..6004
                     /label=Oc-pair2-F
     primer_bind     6024..6045
                     /label=IRES-F
     primer_bind     complement(6035..6054)
                     /label=IRES+300-3
     primer_bind     complement(6204..6229)
                     /label=pR-IRES-3
     primer_bind     complement(6218..6237)
                     /label=IRES-R
     primer_bind     complement(6218..6237)
                     /label=IRES-EcoRI-NcoI-NotI
     primer_bind     complement(6219..6241)
                     /label=IRES2-R
     CDS             6252..6266
                     /codon_start=1
                     /label=ATG
                     /translation="MDRSG"
     primer_bind     6266..6280
                     /label=HindIII-Hyg-5
     CDS             6267..7286
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(6289..6309)
                     /label=Hyg-3
     primer_bind     complement(6608..6618)
                     /label=NheI-NotI-DsRed-5
     primer_bind     6621..6630
                     /label=Kan-deltaEsp3I-R
     primer_bind     6788..6805
                     /label=Hyg-5
     primer_bind     complement(6964..6975)
                     /label=NheI-NotI-DsRed-5
     primer_bind     complement(7265..7286)
                     /label=Hyg-XbaI-3
     primer_bind     complement(7270..7283)
                     /label=Hyg-P2A(23bp)-3
     primer_bind     7547..7557
                     /label=WNT4-1F
     primer_bind     complement(7649..7670)
                     /label=BglII-SV40LT-R
     primer_bind     complement(7683..7702)
                     /label=Octet-MfeI-5
     primer_bind     7691..7718
                     /label=MBB-SV40pA-F
     polyA_signal    7692..7813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7692..7716
                     /label=PB6R-Hprt-F
     primer_bind     complement(7789..7857)
                     /label=pPB-SV40pA-R
     primer_bind     complement(7795..7817)
                     /label=pSp-NH-pA-R
     primer_bind     complement(7812..7835)
                     /label=SV40pA-BamHI-3
     misc_feature    7834..8068
                     /gene="PB3'TR"
                     /label=PB3'TR
     primer_bind     complement(8039..8068)
                     /label=PBF-R
     primer_bind     complement(8042..8068)
                     /label=NcoI-NotI-PBLm-5
     primer_bind     complement(8047..8068)
                     /label=Sfo-cassette-5
     promoter        complement(8088..8106)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8088..8106)
                     /label=T3-5
     primer_bind     complement(8127..8143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8127..8143)
                     /label=M13 rev
     primer_bind     complement(8127..8143)
                     /label=pDEST-M13rev
     primer_bind     complement(8148..8167)
                     /label=Lac ope-5
     protein_bind    8151..8167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8175..8205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8180..8199)
                     /label=Lac pro-5
     protein_bind    8220..8241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8220..8241)
                     /label=CAP-5
     primer_bind     complement(8328..8347)
                     /label=pCRstart-5
     primer_bind     complement(8474..8493)
                     /label=pPB-PciI-R
     primer_bind     8474..8491
                     /label=pSp-XbaI-3
     rep_origin      complement(8529..9117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8529..8548)
                     /label=Ori-end-5
     primer_bind     8780..8796
                     /label=Ori-ApaLI-3
     primer_bind     complement(8787..8806)
                     /label=Ori-ApaLI-5
     primer_bind     complement(8840..8861)
                     /label=Ori-5
     primer_bind     9098..9117
                     /label=Ori-start-3
     primer_bind     complement(9165..9184)
                     /label=AO-5
     CDS             complement(9288..10148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9531..9550
                     /label=Amp-3
     primer_bind     complement(9581..9600)
                     /label=Amp-5
     primer_bind     complement(9762..9781)
                     /label=Amp422-5
     primer_bind     9822..9849
                     /label=Amp-ScaIfus-R
     primer_bind     9842..9861
                     /label=Amp-ScaI-3
     promoter        complement(10149..10253)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10149..10168
                     /label=AmpP-end-3
     primer_bind     complement(10199..10218)
                     /label=AmpP-5
     primer_bind     complement(10203..10253)
                     /label=pAmp2-Link-5
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcgt cgacattgat tattgactag ctagttatta atagtaatca attacggggt
     1021 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     1081 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     1141 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     1201 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     1261 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     1321 agtacatcta cgtattagtc atcgctatta ccatgatggt cgaggtgagc cccacgttct
     1381 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     1441 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg
     1501 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1741 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     1861 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     1921 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     1981 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     2041 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     2101 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     2161 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     2221 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     2281 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     2341 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     2401 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     2461 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     2521 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     2701 aagaattcct cgaggccacc atgtctagac tggacaagag caaagtcata aacggcgctc
     2761 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     2821 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     2881 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
     2941 catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc
     3001 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     3061 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     3121 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     3181 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
     3241 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     3301 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg
     3361 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
     3421 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgagggcagg ggaagtcttc
     3481 taacatgcgg ggacgtggag gaaaatcccg gccccatgag cagcccggat gcgggatacg
     3541 ccagtgacga ccagagccag acccagagcg cgctgcccgc ggtgatggcc gggctgggcc
     3601 cctgcccctg ggccgagtcg ctgagcccca tcggggacat gaaggtgaag ggcgaggcgc
     3661 cggcgaacag cggagcaccg gccggggccg cgggccgagc caagggcgag tcccgtatcc
     3721 ggcggccgat gaacgctttc atggtgtggg ctaaggacga gcgcaagcgg ctggcgcagc
     3781 agaatccaga cctgcacaac gccgagttga gcaagatgct gggcaagtcg tggaaggcgc
     3841 tgacgctggc ggagaagcgg cccttcgtgg aggaggcaga gcggctgcgc gtgcagcaca
     3901 tgcaggacca ccccaactac aagtaccggc cgcggcggcg caagcaggtg aagcggctga
     3961 agcgggtgga gggcggcttc ctgcacggcc tggctgagcc gcaggcggcc gcgctgggcc
     4021 ccgagggcgg ccgcgtggcc atggacggcc tgggcctcca gttccccgag cagggcttcc
     4081 ccgccggccc gccgctgctg cctccgcaca tgggcggcca ctaccgcgac tgccagagtc
     4141 tgggcgcgcc tccgctcgac ggctacccgt tgcccacgcc cgacacgtcc ccgctggacg
     4201 gcgtggaccc cgacccggct ttcttcgccg ccccgatgcc cggggactgc ccggcggccg
     4261 gcacctacag ctacgcgcag gtctcggact acgctggccc cccggagcct cccgccggtc
     4321 ccatgcaccc ccgactcggc ccagagcccg cgggtccctc gattccgggc ctcctggcgc
     4381 cacccagcgc ccttcacgtg tactacggcg cgatgggctc gcccggggcg ggcggcgggc
     4441 gcggcttcca gatgcagccg caacaccagc accagcacca gcaccagcac caccccccgg
     4501 gccccggaca gccgtcgccc cctccggagg cactgccctg ccgggacggc acggacccca
     4561 gtcagcccgc cgagctcctc ggggaggtgg accgcacgga atttgaacag tatctgcact
     4621 tcgtgtgcaa gcctgagatg ggcctcccct accaggggca tgactccggt gtgaatctcc
     4681 ccgacagcca cggggccatt tcctcggtgg tgtccgacgc cagctccgcg gtatattact
     4741 gcaactatcc tgacgtgggg tctggtattc agcaggccac tacaggagtc tcacaagaaa
     4801 cctctgaaaa tcctggtaac aaaacaatag ttcctgcaac gttaccacaa ctcaccccta
     4861 ccctggtgtc actgttggag gttattgaac ctgaagtgtt atatgcagga tatgatagct
     4921 ctgttccaga ctcaacttgg aggatcatga ctacgctcaa catgttagga gggcggcaag
     4981 tgattgcagc agtgaaatgg gcaaaggcaa taccaggttt caggaactta cacctggatg
     5041 accaaatgac cctactgcag tactcctgga tgtttcttat ggcatttgct ctggggtgga
     5101 gatcatatag acaatcaagt gcaaacctgc tgtgttttgc tcctgatctg attattaatg
     5161 agcagagaat gactctaccc tgcatgtacg accaatgtaa acacatgctg tatgtttcct
     5221 ctgagttaca caggcttcag gtatcttatg aagagtatct ctgtatgaaa accttactgc
     5281 ttctctcttc agttcctaag gacggtctga agagccaaga gctatttgat gaaattagaa
     5341 tgacctacat caaagagcta ggaaaagcca ttgtcaagag ggaaggaaac tccagccaga
     5401 actggcagcg gttttatcaa ctgacaaaac tcttggattc tatgcatgaa gtggttgaaa
     5461 atctccttaa ctattgcttc caaacatttt tggataagac catgagtact gaattccccg
     5521 agatgttagc tgaaatcatc accaatcaga taccaaaata ttcaaatgga aatatcaaaa
     5581 aacttctgtt tcatcaaaag gggtctggtt ggtctcatcc gcaattcgag aagtgagcac
     5641 tagaggaatt ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     5701 aataaggccg gtgtgtgttt gtctatatgt tattttccac catattgccg tcttttggca
     5761 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     5821 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     5881 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     5941 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     6001 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     6061 gcgtagtcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggaatctgat
     6121 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     6181 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac
     6241 ccgtaccaaa gatggataga tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt
     6301 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     6361 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     6421 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     6481 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     6541 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     6601 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     6661 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     6721 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     6781 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     6841 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     6901 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     6961 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     7021 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     7081 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     7141 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     7201 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     7261 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc tgatttcgag
     7321 aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga acggccttac
     7381 gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg tcgcgggagg
     7441 gccggtcgca gtgattcagg cccttctgga ttgtgttggt ccccagggca cgattgtcat
     7501 gcccacgcac tcgggtgatc tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc
     7561 cgattgggtg cagatctaga taactgatca taatcagcca taccacattt gtagaggttt
     7621 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7681 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7801 tcaatgtatc ttaacgctcg agtgcatgga tcctttgtta ctttatagaa gaaattttga
     7861 gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
     7921 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
     7981 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
     8041 cacaatatga ttatctttct agggttaaag agctccagct tttgttccct ttagtgaggg
     8101 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8161 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8221 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8281 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8941 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     9001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10261 ccccgaaaag tgccac
//
