LOCUS       Exported                5798 bp ds-DNA     circular SYN 05-JAN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-CreEGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Daekee Lee
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 6, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="/vntifkey=9"
     promoter        365..568
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="/vntifkey=29"
     CDS             670..1698
                     /codon_start=1
                     /label=Cre
                     /note="/vntifkey=4"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     728..747
                     /label=S1
     primer_bind     complement(986..1005)
                     /label=AS1
     CDS             1744..2463
                     /codon_start=1
                     /label=EGFP
                     /note="/vntifkey=4"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2586..2707
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="/vntifkey=25"
     promoter        3302..3659
                     /label=SV40 promoter
                     /label=SV40\promoter
                     /note="/vntifkey=29"
     CDS             3694..4488
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="/vntifkey=4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4720..4767
                     /label=HSV-TK polyA
                     /label=HSV-TK\polyA
                     /note="/vntifkey=25"
     rep_origin      5096..5684
                     /label=ori
                     /note="/vntifkey=33"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggg gcagagccga tcctgtacac tttacttaaa accattatct
      661 gagtgtgaaa tgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat
      721 gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt
      781 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
      841 ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat
      901 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
      961 cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg
     1021 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
     1081 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat
     1141 atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
     1201 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
     1261 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
     1321 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
     1381 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
     1441 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
     1501 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
     1561 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
     1621 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
     1681 ctgctggaag atggcgatgt cgacctcgag ggggggcccg ggatccaccg gccggtcgcc
     1741 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1801 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1861 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1921 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1981 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2041 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2101 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2161 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     2221 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     2281 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2341 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2401 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2461 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     2521 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2581 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2641 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2701 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2761 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2821 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2881 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2941 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3001 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3061 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3121 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3181 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3241 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3301 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3361 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3421 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3481 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3541 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3601 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     3661 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3721 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3781 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3841 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     3901 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3961 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4021 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4081 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4141 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4201 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4261 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4321 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4381 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4441 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4501 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4561 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4621 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     4681 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     4741 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     4801 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     4861 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     4921 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     4981 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5041 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5101 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5161 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5221 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5281 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5341 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5401 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5461 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5521 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5581 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5641 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5701 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5761 cccctgattc tgtggataac cgtattaccg ccatgcat
//
