LOCUS       pBait                   6207 bp    DNA     circular UNA 06-JAN-2021
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local...q-cv0snm9
VERSION     urn.local...q-cv0snm9
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(150..817)
                     /vntifkey=33
                     /label="Col\E1\origin"
     CDS             complement(965..1762)
                     /vntifkey=4
                     /label="KanR"
     motif           join(3776..3792,3795..3811,3814..3830,2286..2302,
                     2305..2321,2324..2340)
                     /created_by="User"
                     /label="UAS"
     motif           <2348..2352
                     /Color="#ffbb33"
                     /created_by="smarajitmondal"
                     /label="KpnI"
     CDS             complement(2364..2669)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
                     /label="ccdB CDS"
     CDS             complement(3011..3670)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
                     /label="CmR"
     promoter        complement(3724..3754)
                     /note="E. coli lac promoter with an """"up"""" mutation"
                     /label="lac UV5 promoter"
     motif           3762..>3766
                     /Color="#ffbb33"
                     /created_by="smarajitmondal"
                     /label="KpnI"
     promoter        3857..4084
                     /created_by="User"
                     /label="Minimal HS promoter"
     CDS             4166..4876
                     /product="M_DasherGFP_*"
                     /translation="MTALTEGAKLFEKEIPYITELEGDVEGMKFIIKGEGTGDATTGT
                     IKAKYICTTGDLPVPWATLVSTLSYGVQCFAKYPSHIKDFFKSAMPEGYTQERTISFE
                     GDGVYKTRAMVTYERGSIYNRVTLTGENFKKDGHILRKNVAFQCPPSILYILPDTVNN
                     GIRVEFNQAYDIEGVTEKLVTKCSQMNRPLAGSAAVHIPRYHHITYHTKLSKDRDERR
                     DHMCLVEVVKAVDLDTYQ*"
                     /modified_by="User"
                     /label="M_DasherGFP_*"
     misc_feature    4922..5020
                     /product="pA_GH-Bt(min)"
                     /Color="#ff7777"
                     /modified_by="User"
                     /label="pA_GH-Bt(min)"
     misc_feature    5057..5200
                     /product="E_SV40"
                     /modified_by="User"
                     /label="E_SV40"
     misc_feature    5201..5373
                     /product="P_SV40E"
                     /label="P_SV40E"
     misc_feature    complement(5201..5325)
                     /product="P_SV40L"
                     /label="P_SV40L"
     misc_feature    complement(5204..5370)
                     /product="Ori_SV40"
                     /label="Ori_SV40"
     motif           complement(5268..5352)
                     /created_by="User"
                     /Color="#ffbb33"
                     /modified_by="User"
                     /label="Minimal SV40 ori"
     CDS             5433..6032
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
                     /Color="#0000aa"
                     /modified_by="User"
                     /label="puro (variant)"
     misc_feature    6032^6033
                     /Original_Bases="CGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAGGAGCGC
                     ACGACCCCATGCATCGGTACCTTTAAGACCAATGACTTACAAGGCAGCTG"
                     /label="CGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAGGAGCGCACGACCCCA
                     TGCATCGGTACCTTTAAGACCAATGACTTACAAGGCAGCTG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    6035..6163
                     /product="pA_SV40E"
                     /Color="#ff7777"
                     /modified_by="User"
                     /label="pA_SV40E"
     misc_feature    6193..6205
                     /Original_Bases="GGTAATACGGTTATCCACAGAA"
                     /label="GGTAATACGGTTATCCACAGAA"
                     /note="Geneious type: Editing History Replacement"
     ligation        6193..6207
                     /label="Ligation"
ORIGIN      
        1 ctttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat
       61 agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg
      121 gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttatta gaaaaattca tccagcagac gataaaacgc aatacgctgg ctatccggtg
     1021 ccgcaatgcc atacagcacc agaaaacgat ccgcccattc gccgcccagt tcttccgcaa
     1081 tatcacgggt ggccagcgca atatcctgat aacgatccgc cacgcccaga cggccgcaat
     1141 caataaagcc gctaaaacgg ccattttcca ccataatgtt cggcaggcac gcatcaccat
     1201 gggtcaccac cagatcttcg ccatccggca tgctcgcttt cagacgcgca aacagctctg
     1261 ccggtgccag gccctgatgt tcttcatcca gatcatcctg atccaccagg cccgcttcca
     1321 tacgggtacg cgcacgttca atacgatgtt tcgcctgatg atcaaacgga caggtcgccg
     1381 ggtccagggt atgcagacga cgcatggcat ccgccataat gctcactttt tctgccggcg
     1441 ccagatggct agacagcaga tcctgacccg gcacttcgcc cagcagcagc caatcacggc
     1501 ccgcttcggt caccacatcc agcaccgccg cacacggaac accggtggtg gccagccagc
     1561 tcagacgcgc cgcttcatcc tgcagctcgt tcagcgcacc gctcagatcg gttttcacaa
     1621 acagcaccgg acgaccctgc gcgctcagac gaaacaccgc cgcatcagag cagccaatgg
     1681 tctgctgcgc ccaatcatag ccaaacagac gttccaccca cgctgccggg ctacccgcat
     1741 gcaggccatc ctgttcaatc atactcttcc tttttcaata ttattgaagc atttatcagg
     1801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1861 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     1921 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     1981 aatcccttat aaatcaaaag aatagaccga gatagggttg agtggccgct acagggcgct
     2041 cccattcgcc attcaggctg cgcaactgtt gggaagggcg tttcggtgcg ggcctcttcg
     2101 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     2161 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgacgt aatacgactc
     2221 actatagggc gaattgaagg aaggccgtca aggccgcata cactgcgggg gctctggaga
     2281 cgcgtcggag gacagtactc cgagcggagg acagtactcc gagcggagga cagtactccg
     2341 aagagcggta ccgagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
     2401 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca
     2461 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
     2521 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt
     2581 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct
     2641 cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga
     2701 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca
     2761 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat
     2821 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata
     2881 cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc
     2941 ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccgg
     3001 atccagatct ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     3061 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     3121 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     3181 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     3241 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     3301 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     3361 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     3421 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     3481 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     3541 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     3601 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     3661 ttttctccat tttagcttcc ttagctcctg aaaatctcga cggatcctaa ctcaaaatcc
     3721 acacattata cgagccggaa gcataaagtg taaagcctgg gggtaccgct cttcacggag
     3781 gacagtactc cgagcggagg acagtactcc gagcggagga cagtactccg agcctctaga
     3841 cggagactct agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt
     3901 caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc
     3961 agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta
     4021 accagcaacc aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat
     4081 acaagaagag aactctgaat agggaattgg gaattcgtta acagatccga tatccgccac
     4141 caagcttctg tctagagccg ccaccatgac tgccctgacc gaaggtgcta agctgtttga
     4201 gaaggagatt ccgtacatca ccgagctgga aggggacgtc gaaggaatga agttcatcat
     4261 caagggagaa ggaaccgggg acgctacgac tggaaccatt aaggccaagt atatctgtac
     4321 cactggagat ctgccagtgc cttgggccac ccttgtgtca accctctcgt atggagtgca
     4381 gtgttttgct aagtacccta gccacattaa ggacttcttc aaatccgcca tgccggaagg
     4441 ttatacccaa gagcgcacca tttcttttga gggagatgga gtgtacaaga cccgcgcgat
     4501 ggtcacctat gagaggggat ctatctacaa ccgggtgact ctgactggag aaaactttaa
     4561 gaaggacggg catattcttc ggaagaatgt cgccttccag tgccctccca gcatccttta
     4621 cattctcccc gacactgtga acaacggaat ccgcgtggag ttcaatcaag cctacgacat
     4681 cgagggggtg acggagaagc tggtgaccaa gtgtagccag atgaatcggc cactggccgg
     4741 ttcagcggct gtccacattc cgcgctacca tcatatcact tatcacacta agctctccaa
     4801 agaccgcgat gagaggagag atcacatgtg cctggtggaa gtggtcaagg ccgtcgatct
     4861 cgatacctat cagtaaacct gctctcaaaa ctgtcttctc agcctcgact gtgccttcta
     4921 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4981 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gaagacagtc agccttgtgg
     5041 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5101 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5161 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5221 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5281 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     5341 ggaggctttt ttggaggtat aggcttttgc aaaaagctct ctggctaact agagaaccca
     5401 ctgcttactg gcttatcgtt taagccgcca ccatgaccga gtacaagccc acggtgcgcc
     5461 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     5521 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     5581 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     5641 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     5701 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     5761 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     5821 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5881 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     5941 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6001 cctggtgcat gacccgcaag cccggtgcct gaccaacttg tttattgcag cttataatgg
     6061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6121 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtcggatgag cggtatcagc
     6181 tcactcaaag gcccctcgag ggccttc
//
