LOCUS       pBait-YY1               4818 bp    DNA     circular UNA 06-JAN-2021
DEFINITION  Gibson Assembly of PCR 2 (dGFP-SV40_prom+ori from pD2) - PCR 3
            (puro from pLKO.1-puro) - PCR 4 (pA_SV40 from pD2) into PCR 1
            (vector backbone from pGeneArt1).
ACCESSION   urn.local...1a-cv0t6zd
VERSION     urn.local...1a-cv0t6zd
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(150..817)
                     /vntifkey=33
                     /label="Col\E1\origin"
     CDS             complement(965..1762)
                     /vntifkey=4
                     /label="KanR"
     motif           join(2286..2302,2305..2321,2324..2340,2387..2403,
                     2406..2422,2425..2441)
                     /created_by="User"
                     /label="UAS"
     intron          2343..2354
                     /Color="#ffe400"
                     /created_by="smarajitmondal"
                     /label="YY1"
     intron          2358..2369
                     /Color="#ffe400"
                     /created_by="smarajitmondal"
                     /label="YY1"
     intron          2373..2384
                     /Color="#ffe400"
                     /created_by="smarajitmondal"
                     /label="YY1"
     promoter        2468..2695
                     /created_by="User"
                     /label="Minimal HS promoter"
     CDS             2777..3487
                     /product="M_DasherGFP_*"
                     /translation="MTALTEGAKLFEKEIPYITELEGDVEGMKFIIKGEGTGDATTGT
                     IKAKYICTTGDLPVPWATLVSTLSYGVQCFAKYPSHIKDFFKSAMPEGYTQERTISFE
                     GDGVYKTRAMVTYERGSIYNRVTLTGENFKKDGHILRKNVAFQCPPSILYILPDTVNN
                     GIRVEFNQAYDIEGVTEKLVTKCSQMNRPLAGSAAVHIPRYHHITYHTKLSKDRDERR
                     DHMCLVEVVKAVDLDTYQ*"
                     /modified_by="User"
                     /label="M_DasherGFP_*"
     misc_feature    3533..3631
                     /product="pA_GH-Bt(min)"
                     /Color="#ff7777"
                     /modified_by="User"
                     /label="pA_GH-Bt(min)"
     misc_feature    3668..3811
                     /product="E_SV40"
                     /modified_by="User"
                     /label="E_SV40"
     misc_feature    3812..3984
                     /product="P_SV40E"
                     /label="P_SV40E"
     misc_feature    complement(3812..3936)
                     /product="P_SV40L"
                     /label="P_SV40L"
     misc_feature    complement(3815..3981)
                     /product="Ori_SV40"
                     /label="Ori_SV40"
     motif           complement(3879..3963)
                     /created_by="User"
                     /Color="#ffbb33"
                     /modified_by="User"
                     /label="Minimal SV40 ori"
     CDS             4044..4643
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
                     /Color="#0000aa"
                     /modified_by="User"
                     /label="puro (variant)"
     misc_feature    4646..4774
                     /product="pA_SV40E"
                     /Color="#ff7777"
                     /modified_by="User"
                     /label="pA_SV40E"
ORIGIN      
        1 ctttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat
       61 agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg
      121 gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttatta gaaaaattca tccagcagac gataaaacgc aatacgctgg ctatccggtg
     1021 ccgcaatgcc atacagcacc agaaaacgat ccgcccattc gccgcccagt tcttccgcaa
     1081 tatcacgggt ggccagcgca atatcctgat aacgatccgc cacgcccaga cggccgcaat
     1141 caataaagcc gctaaaacgg ccattttcca ccataatgtt cggcaggcac gcatcaccat
     1201 gggtcaccac cagatcttcg ccatccggca tgctcgcttt cagacgcgca aacagctctg
     1261 ccggtgccag gccctgatgt tcttcatcca gatcatcctg atccaccagg cccgcttcca
     1321 tacgggtacg cgcacgttca atacgatgtt tcgcctgatg atcaaacgga caggtcgccg
     1381 ggtccagggt atgcagacga cgcatggcat ccgccataat gctcactttt tctgccggcg
     1441 ccagatggct agacagcaga tcctgacccg gcacttcgcc cagcagcagc caatcacggc
     1501 ccgcttcggt caccacatcc agcaccgccg cacacggaac accggtggtg gccagccagc
     1561 tcagacgcgc cgcttcatcc tgcagctcgt tcagcgcacc gctcagatcg gttttcacaa
     1621 acagcaccgg acgaccctgc gcgctcagac gaaacaccgc cgcatcagag cagccaatgg
     1681 tctgctgcgc ccaatcatag ccaaacagac gttccaccca cgctgccggg ctacccgcat
     1741 gcaggccatc ctgttcaatc atactcttcc tttttcaata ttattgaagc atttatcagg
     1801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1861 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     1921 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     1981 aatcccttat aaatcaaaag aatagaccga gatagggttg agtggccgct acagggcgct
     2041 cccattcgcc attcaggctg cgcaactgtt gggaagggcg tttcggtgcg ggcctcttcg
     2101 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     2161 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgacgt aatacgactc
     2221 actatagggc gaattgaagg aaggccgtca aggccgcata cactgcgggg gctctggaga
     2281 cgcgtcggag gacagtactc cgagcggagg acagtactcc gagcggagga cagtactccg
     2341 aggccgccat cttggacgcc gccatcttgg acgccgccat cttggacgga ggacagtact
     2401 ccgagcggag gacagtactc cgagcggagg acagtactcc gagcctctag acggagactc
     2461 tagcgagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
     2521 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
     2581 agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
     2641 caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
     2701 gaactctgaa tagggaattg ggaattcgtt aacagatccg atatccgcca ccaagcttct
     2761 gtctagagcc gccaccatga ctgccctgac cgaaggtgct aagctgtttg agaaggagat
     2821 tccgtacatc accgagctgg aaggggacgt cgaaggaatg aagttcatca tcaagggaga
     2881 aggaaccggg gacgctacga ctggaaccat taaggccaag tatatctgta ccactggaga
     2941 tctgccagtg ccttgggcca cccttgtgtc aaccctctcg tatggagtgc agtgttttgc
     3001 taagtaccct agccacatta aggacttctt caaatccgcc atgccggaag gttataccca
     3061 agagcgcacc atttcttttg agggagatgg agtgtacaag acccgcgcga tggtcaccta
     3121 tgagagggga tctatctaca accgggtgac tctgactgga gaaaacttta agaaggacgg
     3181 gcatattctt cggaagaatg tcgccttcca gtgccctccc agcatccttt acattctccc
     3241 cgacactgtg aacaacggaa tccgcgtgga gttcaatcaa gcctacgaca tcgagggggt
     3301 gacggagaag ctggtgacca agtgtagcca gatgaatcgg ccactggccg gttcagcggc
     3361 tgtccacatt ccgcgctacc atcatatcac ttatcacact aagctctcca aagaccgcga
     3421 tgagaggaga gatcacatgt gcctggtgga agtggtcaag gccgtcgatc tcgataccta
     3481 tcagtaaacc tgctctcaaa actgtcttct cagcctcgac tgtgccttct agttgccagc
     3541 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3601 tcctttccta ataaaatgag gaaattgcat cgaagacagt cagccttgtg gaatgtgtgt
     3661 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3961 tttggaggta taggcttttg caaaaagctc tctggctaac tagagaaccc actgcttact
     4021 ggcttatcgt ttaagccgcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     4081 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     4141 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     4201 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     4261 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     4321 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     4381 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     4441 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     4501 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     4561 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     4621 tgacccgcaa gcccggtgcc tgaccaactt gtttattgca gcttataatg gttacaaata
     4681 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4741 tttgtccaaa ctcatcaatg tatcttatca tgtcggatga gcggtatcag ctcactcaaa
     4801 ggcccctcga gggccttc
//
