LOCUS       pDriver                 5302 bp    DNA     circular UNA 06-JAN-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2v-9kobptd
VERSION     urn.local...2v-9kobptd
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     enhancer        131..434
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /standard_name="CMV enhancer"
     promoter        435..638
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /standard_name="CMV promoter"
     misc_feature    724..2859
                     /product="M_SV40 T antigen_*"
                     /standard_name="SVLT-D2"
                     /modified_by="smarajitmondal"
                     /label="SVLT"
     misc_feature    2910..3008
                     /product="pA_GH-Bt(min)"
                     /standard_name="pA_GH-Bt(min)"
     promoter        3133..3316
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
                     /standard_name="SV40 promoter"
     rep_origin      3167..3302
     rep_origin      complement(3531..4119)
     CDS             complement(4290..5150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="bla CDS"
     promoter        complement(5151..5255)
                     /gene="bla"
                     /note="AmpR promoter"
                     /standard_name="bla promoter"
ORIGIN      
        1 tctagagatc cgactcgagt actaggatcc attaggcggc cgcgtggata accgtattac
       61 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      121 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      181 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      361 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      421 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      481 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      541 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      601 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga
      721 tccatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
      781 gaaaggagtg cctgggggaa tattcctctg atgagaaagg catatttaaa aaaatgcaag
      841 gagtttcatc ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa tactctgtac
      901 aagaaaatgg aagatggagt aaaatatgct catcaacctg actttggagg cttctgggat
      961 gcaactgaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     1021 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     1081 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagatcccaa ggactttcct
     1141 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     1201 gctatttaca ccacaaagga aaaagctgca ctgctataca aaaaaattat ggaaaaattt
     1261 tctgtaacct ttataagtag gcataacagt tacaatcata acatactgtt ttttcttact
     1321 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     1381 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt aactagagat
     1441 ccattttctg ttattgagga aagtttgcct ggtgggttaa aggagcatga ttttaatcca
     1501 gaagaagcag aggaaactaa acaagtgtcc tggaagcttg taacagagta tgcaatggaa
     1561 acaaaatgtg atgatgtgtt gttattgctt gggatgtact tggaatttca gtacagtttt
     1621 gaaatgtgtt taaaatgtat taaaaaagaa cagcccagcc actataagta ccatgaaaag
     1681 cattatgcaa atgctgctat atttgctgac agcaaaaacc aaaaaaccat atgccaacag
     1741 gctgttgata ctgttttagc taaaaagcgg gttgatagcc tacaactaac tagagaacaa
     1801 atgttaacaa acagatttaa tgatcttttg gataggatgg atataatgtt tggttctaca
     1861 ggctctgctg acatagaaga atggatggct ggagttgctt ggctacactg tttgttgccc
     1921 aaaatggatt cagtggtgta tgacttttta aaatgcatgg tgtacaacat tcctaaaaaa
     1981 agatactggc tgtttaaagg accaattgat agtggtaaaa ctacattagc agctgctttg
     2041 cttgaattat gtggggggaa agctttaaat gttaatttgc ccttggacag gctgaacttt
     2101 gagctaggag tagctattga ccagttttta gtagtttttg aggatgtaaa gggcactgga
     2161 ggggagtcca gagatttgcc ttcaggtcag ggaattaata acctggacaa tttaagggat
     2221 tatttggatg gcagtgttaa ggtaaactta gaaaagaaac acctaaataa aagaactcaa
     2281 atatttcccc ctggaatagt caccatgaat gagtacagtg tgcctaaaac actgcaggcc
     2341 agatttgtaa aacaaataga ttttaggccc aaagattatt taaagcattg cctggaacgc
     2401 agtgagtttt tgttagaaaa gaggataatt caaagtggca ttgctttgct tcttatgtta
     2461 atttggtaca gacctgtggc tgagtttgct caaagtattc agagcagaat tgtggagtgg
     2521 aaagagagat tggacaaaga gtttagtttg tcagtgtatc aaaaaatgaa gtttaatgtg
     2581 gctatgggag ttggagtttt agattggcta agaaacagtg atgatgatga tgatgacagc
     2641 caggaaaatg ctgataaaaa tgaagatggt ggggagaaga acatggagga ctcagggcat
     2701 gaaacaggca ttgattcaca gtctcaaggc tcatttcagg cccctcagcc ctcacagtcc
     2761 tcacagtctg ttcatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt
     2821 aaaaaacctt ccccacctcc ccctgaacct gaaacataag aattccctgc tctcaaaact
     2881 gtcttctcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2941 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3001 attgcatcga agacagtcag ccttgtggaa tgtgtgtcag ttagagtagt tcatgtcatc
     3061 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     3121 attgtttaaa ccgtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3181 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3241 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3301 cctaggcttt tgcaaaaagc tcggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5281 gacgtcgacg gatcgggaga tc
//
