LOCUS       PROM5_MprbcL_short_chlo 2246 bp ds-DNA     circular     05-NOV-2020
DEFINITION  .
KEYWORDS    "Plasmid.size:2246" "Insert.length" "Vector.length" "Availability" 
            "Status" "Plasmids Box- Number" "Glycerol stock" "L0 position"
            "Type.of.part" "Description" "Phytozome.ID" "TF.family"
            "MarpolBase.Name" "Original.Name:EF450-rbcL-n  PROM5 Mp rbcL short"
            "Acceptor.plasmid" "Resistance" "Source of part-DNA" "Analysis of
            plasmid (sequence RE)" "Designer:EF" "Builder:EF" "Curator
            (annotations seq schema):EF / SSG" "Which Type IIS" "kit"
            "Shared.with..Relevant.info" "Twist.order.item.id"
            "Twsit.line.item.number" "Twist.Plate" "Twist.Plate.number"
            "Twist.Well" "Yield.ng" "GENEWIZ.Clone.ID" "Genewiz.Order" "Label"
FEATURES             Location/Qualifiers
     misc_feature    301..301
                     /label="here, keep"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    334..379
                     /label="rbcL pro"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    498..498
                     /label="fusion"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(join(1653..2246,1..66))
                     /label="CmR (cat, chloramphenicol acetyltransferase)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Replication_ori complement(767..1355)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    309..312
                     /label="fusion"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      complement(1538..1632)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    313..497
                     /label="Mp rbcL-PROM5 short"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT
       61 CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG
      121 TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG TGCCCGATCA ACTCGAGTGC
      181 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
      241 CGAGGCAGAA TTTCAGATAA AAAAAATCCT TAGCTTTCGC TAAGGATGAT TTCTGGAATT
      301 CggtctcTGG AGTTGATTTA ATAATAAAAT AACTAGGTTG CATTACATAT AAAAAACAAT
      361 ATACAATAAT AATGTTTTAT TATTGGAAAA AATTTTTACT TAAAAAATTT TTTATACAAA
      421 AGAAAAATTA GAAAAAAATT TTTATCGAGC AGACCTCATA CTTGCAAGAA TATTATTTGA
      481 TTTGTAGGGA GGGACTTAat gtGAGAccCT GCAGTCCGGC AAAAAAGGGC AAGGTGTCAC
      541 CACCCTGCCC TTTTTCTTTA AAACCGAAAA GATTACTTCG CGTTATGCAG GCTTCCTCGC
      601 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
      661 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG
      721 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CACAGGCTCC
      781 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
      841 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
      901 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
      961 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
     1021 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
     1081 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
     1141 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
     1201 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
     1261 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     1321 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
     1381 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
     1441 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
     1501 TATATGAGTA AACTTGGTCT GACAGCTCGA GGCTTGGATT CTCACCAATA AAAAACGCCC
     1561 GGCGGCAACC GAGCGTTCTG AACAAATCCA GATGGAGTTC TGAGGTCATT ACTGGATCTA
     1621 TCAACAGGAG TCCAAGCGAG CTCGATATCA AATTACGCCC CGCCCTGCCA CTCATCGCAG
     1681 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA
     1741 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG
     1801 AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC
     1861 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC
     1921 AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG
     1981 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA
     2041 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGAAATTCC
     2101 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA
     2161 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA
     2221 CATTGAGCAA CTGACTGAAA TGCCTC
//