LOCUS       Exported               11277 bp ds-DNA     circular SYN 12-ENE-2021
DEFINITION  pEMPTY::sgRNA2, Escherichia coli-Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria
ACCESSION   MW367468
VERSION     .
KEYWORDS    pEMPTY::sgRNA2
SOURCE      Escherichia coli-Staphylococcus aureus
  ORGANISM  .
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Laboratory of Microbial Pathogenesis
  TITLE     .
  JOURNAL   .
            .
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Escherichia coli-Staphylococcus aureus"
                     /mol_type="other DNA"
     CDS             280..930
                     /codon_start=1
                     /product="cat”
                     /note="cat"
                     /protein_id="AMR43721.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     promoter        1270..1374
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1375..2235
                     /codon_start=1
                     /product="beta lactamase"
                     /note="beta lactamase"
                     /note="involved in ampicillin resistance"
                     /protein_id="AMR43722.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2406..2994
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3145..3180
                     /note="Prom phage SP01"
     misc_feature    3181..3207
                     /note=“gRNA2"
     misc_RNA        3208..3283
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      3364..3411
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3460..7566)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     regulatory      complement(7591..7733)
                     /note="dcas9; xyl/tetO&tetR, ATc inducible"
     CDS             7737..8360
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /note="TetR"
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
ORIGIN
        1 cacctaggta ttatcaagat aagaaagaaa aggatttttc gctacgctca aatcctttaa
       61 aaaaacacaa aagaccacat tttttaatgt ggtcttttat tcttcaacta aagcacccat
      121 tagttcaaca aacgaaaatt ggataaagtg ggatattttt aaaatatata tttatgttac
      181 agtaatattg acttttaaaa aaggattgat tctaatgaag aaagcagaca agtaagcctc
      241 ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattgat
      301 ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt
      361 agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat
      421 aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt
      481 agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga gccactttat
      541 acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac
      601 ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa
      661 ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat tccatggact
      721 tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct acccattatt
      781 acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta
      841 catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg
      901 tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca
      961 gtcggttttc taatgtcact aacctgcccc gttagttgaa gaaggccgcg gcctcgagcg
     1021 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     1081 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     1141 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     1201 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1261 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1321 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1381 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1441 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1501 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1561 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1621 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1681 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1741 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1801 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1861 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1921 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1981 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2041 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2101 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2161 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2221 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2281 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2341 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2401 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2461 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2521 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2581 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2641 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2701 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2761 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2821 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2881 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2941 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3001 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3061 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctggcggc
     3121 cgctgcatgc ctgcagggcg cgccttgaca aattgcagta ggcatgacaa aatggactca
     3181 caagttttgg gattgttaag ggttccggtt ttagagctag aaatagcaag ttaaaataag
     3241 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt cgcaaaaaac
     3301 cccgcttcgg cggggttttt tcgctagtcg gtgtgcgcaa ccttaagccc gggtaccaga
     3361 tctcaaaaaa cccctcaaga cccgtttaga ggccccaagg ggttatgcta gttattgctc
     3421 agcggtggca gcagcctagg ataccggtaa catgggatct cagtcacctc ctagctgact
     3481 caaatcaatg cgtgtttcat aaagaccagt gatggattga tggataagag tggcatctaa
     3541 aacttctttt gtagacgtat atcgtttacg atcaattgtt gtatcaaaat atttaaaagc
     3601 agcgggagct ccaagattcg tcaacgtaaa taaatgaata atattttctg cttgttcacg
     3661 tattggtttg tctctatgtt tgttatatgc actaagaact ttatctaaat tggcatctgc
     3721 taaaataaca cgcttagaaa attcactgat ttgctcaata atctcatcta aataatgctt
     3781 atgctgctcc acaaacaatt gtttttgttc gttatcttct ggactaccct tcaacttttc
     3841 ataatgacta gctaaatata aaaaattcac atatttgctt ggcagagcca gctcatttcc
     3901 tttttgtaat tctccggcac tagccagcat ccgtttacga ccgttttcta actcaaaaag
     3961 actatattta ggtagtttaa tgattaagtc ttttttaact tccttatatc ctttagcttc
     4021 taaaaagtca atcggatttt tttcaaagga acttctttcc ataattgtga tccctagtaa
     4081 ctctttaacg gattttaact tcttcgattt ccctttttcc accttagcaa ccactaggac
     4141 tgaataagct accgttggac tatcaaaacc accatatttt tttggatccc agtctttttt
     4201 acgagcaata agcttgtccg aatttctttt tggtaaaatt gactccttgg agaatccgcc
     4261 tgtctgtact tctgttttct tgacaatatt gacttggggc atggacaata ctttgcgcac
     4321 tgtggcaaaa tctcgccctt tatcccagac aatttctcca gtttccccat tagtttcgat
     4381 tagagggcgt ttgcgaatct ctccatttgc aagtgtaatt tctgttttga agaagttcat
     4441 gatattagag taaaagaaat attttgcggt tgctttgcct atttcttgct cagacttagc
     4501 aatcatttta cgaacatcat aaactttata atcaccatag acaaactccg attcaagttt
     4561 tggatatttc ttaatcaaag cagttccaac gacggcattt agatacgcat catgggcatg
     4621 atggtaattg ttaatctcac gtactttata gaattggaaa tcttttcgga agtcagaaac
     4681 taatttagat tttaaggtaa tcactttaac ctctcgaata agtttatcat tttcatcgta
     4741 tttagtattc atgcgactat ccaaaatttg tgccacatgc ttagtgattt ggcgagtttc
     4801 aaccaattgg cgtttgataa aaccagcttt atcaagttca ctcaaacctc cacgttcagc
     4861 tttcgttaaa ttatcaaact tacgttgagt gattaacttg gcgtttagaa gttgtctcca
     4921 atagtttttc atctttttga ctacttcttc acttggaacg ttatccgatt taccacgatt
     4981 tttatcagaa cgcgttaaga ccttattgtc tattgaatcg tctttaagga aactttgtgg
     5041 aacaatgtga tcgacatcat aatcacttaa acgattaata tctaattctt ggtccacata
     5101 catgtctctt ccattttgga gataatagag atagagcttt tcattttgca attgagtatt
     5161 ttcaacagga tgctctttaa gaatctgact tcctaattct ttgatacctt cttcgattcg
     5221 tttcatacgc tctcgcgaat ttttctggcc cttttgagtt gtctgatttt cacgtgccat
     5281 ttcaataacg atattttctg gcttatgccg ccccattact ttgaccaatt catcaacaac
     5341 ttttacagtc tgtaaaatac cttttttaat agcagggcta ccagctaaat ttgcaatatg
     5401 ttcatgtaaa ctatcgcctt gtccagacac ttgtgctttt tgaatgtctt ctttaaatgt
     5461 caaactatca tcatggatca gctgcataaa attgcgattg gcaaaaccat ctgatttcaa
     5521 aaaatctaat attgttttgc cagattgctt atccctaata ccattaatca attttcgaga
     5581 caaacgtccc caaccagtat aacggcgacg tttaagctgt ttcatcacct tatcatcaaa
     5641 gaggtgagca tatgttttaa gtctttcctc aatcatctcc ctatcttcaa ataaggtcaa
     5701 tgttaaaaca atatcctcta agatatcttc attttcttca ttatccaaaa aatctttatc
     5761 tttaataatt tttagcaaat catggtaggt acctaatgaa gcattaaatc tatcttcaac
     5821 tcctgaaatt tcaacactat caaaacattc tatttttttg aaataatctt cttttaattg
     5881 cttaacggtt acttttcgat ttgttttgaa gagtaaatca acaatggctt tcttctgttc
     5941 acctgaaaga aatgctggtt ttcgcattcc ttcagtaaca tatttgacct ttgtcaattc
     6001 gttataaacc gtaaaatact cataaagcaa actatgtttt ggtagtactt tttcatttgg
     6061 aagattttta tcaaagtttg tcatgcgttc aataaatgat tgagctgaag cacctttatc
     6121 gacaacttct tcaaaattcc atggggtaat tgtttcttca gacttccgag tcatccatgc
     6181 aaaacgacta ttgccacgcg ccaatggacc aacataataa ggaattcgaa aagtcaagat
     6241 tttttcaatc ttctcacgat tgtcttttaa aaatggataa aagtcttctt gtcttctcaa
     6301 aatagcatgc agctcaccca agtgaatttg atggggaata gagccgttgt caaaggtccg
     6361 ttgcttgcgc agcaaatctt cacgatttag tttcaccaat aattcctcag taccatccat
     6421 tttttctaaa attggtttga taaatttata aaattcttct tggctagctc ccccatcaat
     6481 ataacctgca tatccgtttt ttgattgatc aaaaaagatt tctttatact tttctggaag
     6541 ttgttgtcga actaaagctt ttaaaagagt caagtcttga tgatgttcat cgtagcgttt
     6601 aatcattgaa gctgataggg gagccttagt tatttcagta tttactctta ggatatctga
     6661 aagtaaaata gcatctgata aattcttagc tgccaaaaac aaatcagcat attgatctcc
     6721 aatttgcgcc aataaattat ctaaatcatc atcgtaagta tcttttgaaa gctgtaattt
     6781 agcatcttct gccaaatcaa aatttgattt aaaattaggg gtcaaaccca atgacaaagc
     6841 aatgagattc ccaaataagc catttttctt ctcaccgggg agctgagcaa tgagattttc
     6901 taatcgtctt gatttactca atcgtgcaga aagaatcgct ttagcatcta ctccacttgc
     6961 gttaataggg ttttcttcaa ataattgatt gtaggtttgt accaactgga taaatagttt
     7021 gtccacatca ctattatcag gatttaaatc tccctcaatc aaaaaatgac cacgaaactt
     7081 aatcatatgc gctaaggcca aatagattaa gcgcaaatcc gctttatcag tagaatctac
     7141 caattttttt cgcagatgat agatagttgg atatttctca tgataagcaa cttcatctac
     7201 tatatttcca aaaataggat gacgttcatg cttcttgtct tcttccacca aaaaagactc
     7261 ttcaagtcga tgaaagaaac tatcatctac tttcgccatc tcatttgaaa aaatctcctg
     7321 tagataacaa atacgattct tccgacgtgt ataccttcta cgagctgtcc gtttgagacg
     7381 agtcgcttcc gctgtctctc cactgtcaaa taaaagagcc cctataagat tttttttgat
     7441 actgtggcgg tctgtatttc ccagaacctt gaacttttta gacggaacct tatattcatc
     7501 agtgatcacc gcccatccga cgctatttgt gccgatatct aagcctattg agtatttctt
     7561 atccataaat aatcatcctc ctaaagatct caagcttatt ttaattatac tctatcaatg
     7621 atagagtgtc aatatttttt ttagtttttc atgaactcga ggggatccaa ataaaaaact
     7681 agtttgacaa ataactctat caatgatata atgtcaacaa aaaggaggaa ttaatgatgt
     7741 ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
     7801 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
     7861 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
     7921 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
     7981 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
     8041 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
     8101 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
     8161 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
     8221 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
     8281 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
     8341 aatgtgaaag tgggtcttaa aagcagcata acctttttcc gtgatggtaa cttcacggta
     8401 accaagatgt cgagttatcg ataccgtcga cctggaattc actggccgtc gttttacaac
     8461 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8521 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8581 gcctgaatgg cgaatggcgc ctgagggttg ccagagttaa aggatcaagt aatacaaacg
     8641 ggatacaaag acataatcaa agagagaata aaaactataa taataaagac ataaatcatg
     8701 aggaaacata taaaaattat gatttgatta acgcacaaaa tataaagtat aaagataaaa
     8761 ttgatgaaac gattgatgag aattattcag ggaaacgtaa aattcggtca gatgcaattc
     8821 gacatgtgga cggactggtt acaagtgata aagatttctt tgatgattta agcggagaag
     8881 aaatagaacg attttttaaa gatagcttgg agtttctaga aaatgaatac ggtaaggaaa
     8941 atatgctgta tgcgactgtc catctggatg aaagagtccc acatatgcac tttggttttg
     9001 tccctttaac agaggacggg agattgtctg caaaagaaca gttaggcaac aagaaagact
     9061 ttactcaatt acaagataga tttaatgagt atgtgaatga gaaaggttat gaacttgaaa
     9121 gaggcacgtc caaagaggtt acagaacgag aacataaagc gatggatcag tacaagaaag
     9181 atactgtatt tcataaacag gaactgcaag aagttaagga tgagttacag aaggcaaata
     9241 agcagttaca gagtggaata gagcatatga ggtctacgaa accctttgat tatgaaaatg
     9301 agcgtacagg tttgttctct ggacgtgaag agactggtag aaagatatta actgctgatg
     9361 aatttgaacg cctgcaagaa acaatctctt ctgcagaacg gattgttgat gattacgaaa
     9421 atattaagag cacagactat tacacagaaa atcaagaatt aaaaaaacgt agagagagtt
     9481 tgaaagaagt agtgaataca tggaaagagg ggtatcacga aaaaagtaaa gaggttaata
     9541 aattaaagcg agagaatgat agtttgaatg agcagttgaa tgtatcagag aaatttcaag
     9601 ctagtacagt gactttatat cgtgctgcga gggcgaattt ccctgggttt gagaaagggt
     9661 ttaataggct taaagagaaa ttctttaatg attccaaatt tgagcgtgtg ggacagttta
     9721 tggatgttgt acaggataat gtccagaagg tcgatagaaa gcgtgagaaa cagcgtacag
     9781 acgatttaga gatgtagagg tacttttatg ccgagaaaac tttttgcgtg tgacagtcct
     9841 taaaatatac ttagagcgta agcgaaagta gtagcgacag ctattaactt tcggttgcaa
     9901 agctctagga tttttaatgg acgcagcgca tcacacgcaa aaaggaaatt ggaataaatg
     9961 cgaaatttga gatgttaatt aaagaccttt ttgaggtctt tttttcttag atttttgggg
    10021 ttatttaggg gagaaaacat aggggggtac tacgacctcc cccctaggtg tccattgtcc
    10081 attgtccaaa caaataaata aatattgggt ttttaatgtt aaaaggttgt tttttatgtt
    10141 aaagtgaaaa aaacagatgt tgggaggtac agtgatagtt gtagatagaa aagaagagaa
    10201 aaaagttgct gttactttaa gacttacaac agaagaaaat gagatattaa atagaatcaa
    10261 agaaaaatat aatattagca aatcagatgc aaccggtatt ctaataaaaa aatatgcaaa
    10321 ggaggaatac ggtgcatttt aaacaaaaaa agatagacag cactggcatg ctgcctatct
    10381 atgactaaat tttgttaagt gtattagcac cgttattata tcatgagcga aaatgtaata
    10441 aaagaaactg aaaacaagaa aaattcaaga ggacgtaatt ggacatttgt tttatatcca
    10501 gaatcagcaa aagccgagtg gttagagtat ttaaaagagt tacacattca atttgtagtg
    10561 tctccattac atgataggga tactgataca gaaggtagga tgaaaaaaga gcattatcat
    10621 attctagtga tgtatgaggg taataaatct tatgaacaga taaaaataat taacagaaga
    10681 attgaatgcg actattccgc agattgcagg aagtgtgaaa ggtcttgtga gatatatgct
    10741 tcacatggac gatcctaata aatttaaata tcaaaaagaa gatatgatag tttatggcgg
    10801 tgtagatgtt gatgaattat taaagaaaac aacaacagat agatataaat taattaaaga
    10861 aatgattgag tttattgatg aacaaggaat cgtagaattt aagagtttaa tggattatgc
    10921 aatgaagttt aaatttgatg attggttccc gcttttatgt gataactcgg cgtatgttat
    10981 tcaagaatat ataaaatcaa atcggtataa atctgaccga tagattttga atttaggtgt
    11041 cacaagacac tcttttttcg caccagcgaa aactggttta agccgactgc gcaaaagaca
    11101 taatcgataa gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac
    11161 cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg
    11221 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatcga gggggcc
//
