LOCUS       #673-Lon_Protease_Compl 7867 bp ds-DNA     circular     19-MAY-2020
DEFINITION  .
KEYWORDS    "accession:urn; local; 31odq5x-38" "marker:KmR"                    
FEATURES             Location/Qualifiers
     promoter        complement(5801..5829)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(6421..6999)
                     /label="NS2b"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             complement(join(7054..7867,1..2))
                     /label="KmR"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     gene            436..2799
                     /label="Lon Protease"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      2846..3252
                     /label="rrnB"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    301..306
                     /label="-35"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    422..428
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(6346..6420)
                     /label="lacZ_a"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_binding    334..356
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3260..3904)
                     /label="NS2a"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rRNA            379..416
                     /label="Riboswitch-E"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        281..336
                     /label="Ptrc\promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    336..356
                     /label="O1\operator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(4122..4804)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     intron          417..421
                     /label="Spacer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    324..329
                     /label="-10"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            complement(4899..5759)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(6022..6328)
                     /label="f1_origin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          3258..3280
                     /label="JKS142"
                     /note="sequence: GCGGTTGCTACCAAGTAGTGACC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          1963..1984
                     /label="JKS5"
                     /note="sequence: atcgagaaggtcaaaatcgcgc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3217..3238)
                     /label="JKS178"
                     /note="sequence: AAAAGGCCATCCGTCAGGATGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          86..109
                     /label="JKS1"
                     /note="sequence: agagattttgagacacaacgtggc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3003..3024)
                     /label="JKS6"
                     /note="sequence: cagttccctactctcgcatggg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3258..3280)
                     /label="NS2R"
                     /note="sequence: GGTCACTACTTGGTAGCAACCGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1030..1051
                     /label="JKS3"
                     /note="sequence: tttgcggaagacgggatcatca"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1076..1097)
                     /label="JKS2"
                     /note="sequence: atctcgttcttttgtcgtgccg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2045..2066)
                     /label="JKS4"
                     /note="sequence: tcattaatagcgccttccgtga"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          6969..6990
                     /label="NS2F"
                     /note="sequence: CTGAGAGGGTGTAGCCCGTCAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
       61 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
      121 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt
      181 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc
      241 aaccgtggct ccctcacttt ctggctggat gatggaattc aaatattctg aaatgagctg
      301 ttgacaatta atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcat
      361 acgctcacaa ttggtaccgg tgataccagc atcgtcttga tgcccttggc agcaccctgc
      421 taaggaggca acaagatgag caagaagatt aagctgccca tttttcagat tcgtggttcg
      481 tttatcgtcc ccggcattaa ggaaaacctc gaagtcggcc gaaagaacac cctggccagt
      541 gtcaattatg ctatcaaaaa ttcgaataac cagatgatcg ccatcccaca aatcgatgcc
      601 agcgttgaaa aacccgagtt cagcgacctg cacgagttcg ggattctcat tgattttgaa
      661 gttatcaagg aatggaaaga caattccctg accatttcga ccaaccccat tcagcggtgc
      721 aaagtcatca gcttctttga gaacgaagat caggtgccat acgcggaggt cgagctcatt
      781 gagagtatca acgacttttc tgatgaagaa ttgaaagaac tgattgaaaa aattagtgac
      841 gccatcaaga ctaaagcgtc gctcgtgacg aaacaaatta agcaattgat ctcgggtgaa
      901 agtgacgatc tgtcgttggc gttcgattcg atcatgttta aactggcgcc gtctaagatc
      961 ctcactaatc ccgagtatat tacatctccc agtctcaaga cacgctggtc gatcattgaa
     1021 aaaattattt ttgcggaaga cgggatcatc acccggaatg ctgaatccat tgacgcggca
     1081 cgacaaaaga acgagattga acaagagctg aaccataaat tgaaagaaaa aatggacaag
     1141 cagcagaagg agtactatct gcgcgagaag atgcggatta tcaaggacga gttggaagat
     1201 gaagatgact ccgatgacag ttctctggag aaatataagg agcgcttggc gaaagagccc
     1261 ttccccgaag aggtcaaacg aaagattatg gcttcgatca agcgcgtcga agccctgcaa
     1321 tctggaaccc ccgaatggaa tactgagaag aactacattg actggatgat gagtattcct
     1381 tggtgggaag aaacggagga tctgaccgat ttgaagtacg ctaaaaagat cctggacaag
     1441 catcattacg gaatgaaaaa agtgaaagag cgaatcatcg aatatctcgc tgtgaaaacg
     1501 aaaaccaaga gcctgaaggc ccctatcatt acactggtcg gtcctccggg cgttgggaaa
     1561 actagcttgg cgaaaagtat cgctgaggca gtcggcaaaa attttgtcaa ggtgagcttg
     1621 ggtggtgtta aagacgagag cgaaatccgg ggtcatcgga aaacatatgt cggttcgatg
     1681 cccggccgta tcatccaaac catgaagcgg gccaaagtga aaaacccatt gtttctcctc
     1741 gatgaaatcg ataaaatggc ttctgatcat cgcggggacc cagcatccgc catgctcgag
     1801 gtgctggatc ccgagcaaaa caaagaattt tcggatcact atattgaaga accttacgat
     1861 ctctctcagg ttatgttcat tgcgacagca aattacccag aggatatccc cgaagccctg
     1921 tatgatcgca tggagatcat taatctgtcg agctacactg agatcgagaa ggtcaaaatc
     1981 gcgcaagact atctggtgcc caaggcaatt gaacagcacg aattgacgtc tgaggaaatc
     2041 tccttcacgg aaggcgctat taatgaaatt attaagtact atacccggga ggccggcgtc
     2101 cgacaactcg aacgtcatat caacagcatt attcggaaat atattgtcaa aaatttgaac
     2161 ggtgagatgg ataagattgt gatcgacgaa aagcaagtta atgatttgct ggggaaacgc
     2221 attttcgatc atactgaaaa gcaggaggaa tctcaaattg gcgtggtcac cggcctggcc
     2281 tacacgcagt ttggcggtga tattttgcct atcgaagtgt ctctgtaccc cggcaagggc
     2341 aatctcattt tgacggggaa actgggcgag gtgatgaaag aatcggcgac tattgcgctg
     2401 acctatgtta aatcgaactt cgaaaaattc ggtgtggaca aaaaagtgtt tgaagagaat
     2461 gatatccatg tccacgtccc cgagggtgca gtgccaaaag acggccccag cgcgggaatt
     2521 accattacaa ccgccctcat cagcgcattg agtgataagc ctgtttccaa agagattggt
     2581 atgacgggtg aaattacgct gcgaggaaac gtgctgccca tcggaggttt gcgggagaag
     2641 tcgatttctg ccagccgtag cggtctcaag acgattatta ttccaaaaaa aaatgaacgc
     2701 gatttggatg agattccgga cgaggtcaaa gcgaagctca agatcatccc cgccgaaaag
     2761 tatgaagagg tgttcgctat tgtttttaag accaagtaat tctagagtcg acctgcaggc
     2821 atgcaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
     2881 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     2941 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     3001 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     3061 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     3121 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     3181 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     3241 cgtttctaca aactcttgcg gttgctacca agtagtgacc cgcttcgtga tgcaaaatcc
     3301 gctgacgata ttcgggcgat cgctgctgaa tgccatcgag cagtaacgtg gcaccccgcc
     3361 cctgccaagt caccgcatcc agactgaaca gcaccaagag gctaaaaccc aatcccgccg
     3421 gtagcagcgg agaactaccc agcattggtc ccaccaaagc taatgccgtc gtggtaaaaa
     3481 tcgcgatcgc cgtcagactc aagcccagtt cgctcatgct tcctcatcta ggtcacagtc
     3541 ttcggcgatc gcatcgatct gatgctgcag caagcgtttt ccataccggc gatcgcgccg
     3601 tcgccctttc gctgccgtgg cccgcttacg agctcgttta tcgaccacga tcgcatccaa
     3661 atccgcgatc gcttcccagt ccggcaattc agtctggggc gtccgtttca ttaatcctga
     3721 tcaggcacga aattgctgtg cgtagtatcg cgcatagcgg ccagcctctg ccaacagcgc
     3781 atcgtgattg cctgcctcaa caatctggcc gcgctccatc accaagatgc ggctggcatt
     3841 acgaaccgta gccagacggt gagcaatgat aaagaccgtc cgtccctgca tcacccgttc
     3901 taggtgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4561 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4801 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4861 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4921 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4981 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5041 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5101 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5161 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5221 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5281 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5341 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5401 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5461 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5521 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5581 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5641 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5701 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5761 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5821 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5881 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     5941 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6001 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     6061 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     6121 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     6181 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     6241 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     6301 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     6361 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     6421 ctggcttaca gggataactg agagtcaaca gcctctgtcc gtcattgcac acccatccat
     6481 gcactgggga cttgactcat gctgaatcac atttcccttg tccattgggc gagaggggag
     6541 gggaatcttc tggactcttc actaagcggc gatcgcaggt tcttctaccc aagcagtggc
     6601 gatcgcttga ttgcagtctt caatgctggc ctctgcagcc atcgccgcca ccaaagcatc
     6661 gtaggcggga cgttgttgct ccagtaaagt cttcgcccgt aacaatcccc agcgactgcg
     6721 taaatccgct tcggcaggat tgcgatcgag ttgccgccac agttgtttcc actgggcgcg
     6781 atcgtcagct cccccttcca cgttgccgta gaccagttgc tctgccgctg caccggccat
     6841 caacacctga caccactgtt ccagcgatcg ctgactgagt tgcccctgtg cggcttcggc
     6901 ttctagcgca gctgcttgga actgcacacc cccgcgacca ggttgtcctt ggcgcagcgc
     6961 ttcccacgct gagagggtgt agcccgtcac gggtaactcc cgtcaagtca gcgtaatgct
     7021 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     7081 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     7141 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     7201 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     7261 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
     7321 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     7381 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     7441 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     7501 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     7561 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     7621 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     7681 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     7741 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     7801 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat
     7861 atggctc
//