LOCUS       NS3-mNG-PDT#3           5634 bp ds-DNA     circular     07-OCT-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             2359..2442
                     /label="PDT#3"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    1558..1629
                     /label="promoter region"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1609..1629
                     /label="O1 operator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     exon            1651..2442
                     /label="Insert"
                     /ApEinfo_revcolor=#708090
                     /ApEinfo_fwdcolor=#708090
     misc_feature    1558..1558
                     /label="ideal operator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2451..5406
                     /label="pNS3_3B_corrected for mutations"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2558..3217
                     /label="CmR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(4498..5397)
                     /label="NS3I"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            1651..2358
                     /label="mNeonGreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2451..2455
                     /label="MCS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      join(5412..5634,1..397)
                     /label="pUC57 origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      1338..1381
                     /label="rrnB Terminator T1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      1513..1540
                     /label="rrnB Terminator T2"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    join(5407..5634,1..1650)
                     /label="pNS3_3B_corrected for mutations"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(427..1326)
                     /label="NS3II"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      4443..4491
                     /label="trp terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1637..1642
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            3360..4442
                     /label="lacI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1597..1602
                     /label="-10 lac UV5"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1648..1650
                     /label="MCS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1573..1578
                     /label="-35 trp"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          164..209
                     /label="JKS122"
                     /note="sequence: gcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(2334..2358)
                     /label="JKS129"
                     /note="sequence: ccagcattcagcataaacgtcttgtacagttcgtccatacccatc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2794..2815)
                     /label="JKS49"
                     /note="sequence: atcccatatcaccagctcaccg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2456..2479
                     /label="JKS128"
                     /note="sequence: gacgacgggtctaaGaattcGATCGGCACGTAAGAGGTTCCAAC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1103..1123
                     /label="JKS48"
                     /note="sequence: cgcgggaatcgtagcaaagtc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(139..183)
                     /label="JKS123"
                     /note="sequence: gcctacatacctcgctctgctaatcctgttaccagtggctgctgc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2440..2465
                     /label="PDT Uni F"
                     /note="sequence: TAAAAGCGTAGAATTCGATCGGCACG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(2382..2416)
                     /label="PDT Mod BB R"
                     /note="sequence: CAGCATTCAGCATAAACGTCGGAACTTCGTTTGTG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2456..2486
                     /label="JKS130"
                     /note="sequence: GATCGGCACGTAAGAGGTTCCAACTTTCACC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
       61 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      121 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      181 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      241 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      301 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      361 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      421 tctagttcag ccagctcgtc gtgatgtcga aacccaagcc accctcagag gtgaaggccg
      481 cttcgagcac atcgggaagc gtgtcgacga cggtgctcgg tgcctcgggt aagagccaga
      541 tagatgcggt gacgttcacc gtcgcgatcg tggcgggaac cacggtcact gcgtccgtta
      601 caactcgcac gtcgtcggcc agcacgactg actcgacggc ttcgattagc ccgggagagg
      661 caaggccatc aggctcggta gacagaatgc tgattaacac ctcgccggga gcagggctgc
      721 tcacagccgc gtccttcacc cgtgggtcag ccgtcagcgc ttgatagcgg taccaggccg
      781 caccgcctgc ggtcgagctg cccttgatcc gctcgatggt gcgatcgcga agctcgccat
      841 ccggctcatt cggctgccgc accacgccgt agaacgcggc aaggttgtcg aggtcggggc
      901 cgtttgagta gcgaagcagt gtcgcaagaa gtgcgtcgtt gatccgctga cgcaggatca
      961 gctcgcgggc tgcgcagacc tccagcagct tgatgaccgg gtcggattcg aggatggcgg
     1021 tgtagctggc gtcacgcgat cgcaggtcgt cgatcaagtc ctgcaggatc agttcaaagt
     1081 ccagcgcttc gatgatggtg ggcgcgggaa tcgtagcaaa gtcaagaacg gtcatgagac
     1141 gactaagccc tccagcgtga tacgcctgcc ctcggggatg tagtagccga tcaggttcag
     1201 ctcgacttga ccagctgcgc tggctgagac gatgcgaacc ttctccagct tcagccgtgg
     1261 ctcccagcga tcgagcgctt cagctgtggc cgccaccagg tcaacgatga gggactggtt
     1321 gatcggctag tcatcaaata aaacgaaagg ctcagtcgga agactgggcc tttcgtttta
     1381 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     1441 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
     1501 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta ctctagtttc
     1561 gaaaccccag gcttgacact ttatgcttcc ggctcgtata atgtgtggaa ttgtgagcgg
     1621 ataacaattt cacacaagga ggaaaaacat atggtcagca aaggtgaaga agacaatatg
     1681 gcgagcctgc cggcgacgca tgaactgcac attttcggta gcatcaacgg tgtggacttc
     1741 gatatggttg gccagggtac cggcaacccg aatgacggtt atgaagaact gaatctgaaa
     1801 agtacgaagg gcgatctgca attttccccg tggattctgg tcccgcatat cggttacggc
     1861 ttccaccagt acctgccgta tccggatggt atgagcccgt ttcaagcggc catggtggac
     1921 ggttctggct atcaggttca tcgtaccatg caattcgaag atggcgcgag tctgaccgtg
     1981 aactatcgct acacgtatga aggttcacac attaaaggcg aagcgcaggt caaaggtacc
     2041 ggttttccgg cggatggtcc ggtgatgacc aactcactga cggcagctga ttggtgccgt
     2101 tcgaaaaaga cctatccgaa tgacaaaacc attatcagca cgtttaagtg gtcttacacc
     2161 acgggtaacg gcaaacgtta tcgcagcacc gcacgtacca cgtacacgtt cgctaaaccg
     2221 atggcggcca actacctgaa aaatcagccg atgtatgttt ttcgcaaaac cgaactgaaa
     2281 cattccaaga cggaactgaa cttcaaggaa tggcaaaagg cgtttacgga tgtgatgggt
     2341 atggacgaac tgtacaaggc tgctaataaa aacgaagaga acacaaacga agttccgacg
     2401 tttatgctga atgctggcca agcaaaccga cgacgggtct aaAAGCGTAG gatccgatcg
     2461 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     2521 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     2581 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     2641 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     2701 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     2761 tgctcatccg gagttccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     2821 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     2881 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     2941 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     3001 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     3061 ccccgttttc actatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     3121 gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     3181 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
     3241 cccttgaatt cctactagtc gaagcggcat gcatttacgt tgacaccatc gaatggtgca
     3301 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg
     3361 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     3421 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     3481 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     3541 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     3601 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     3661 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     3721 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     3781 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     3841 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     3901 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     3961 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     4021 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     4081 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     4141 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     4201 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     4261 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     4321 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     4381 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4441 gatcccacag ccgccagttc cgctggcggc attttaactt tctttaatga atctagtgac
     4501 aagccggggc agacgtgagc cgtagtcccg tcgccagacg cgggtgccca cgggcgtcgt
     4561 caggatgtcc gtaattgact gccggaggtg gtcaatgccc ttcagctcct tgccactgtc
     4621 acggctcatg cctcgggtca ttagtcgccc gctccggtat cttcactggc ttcgatgatt
     4681 gccgccccgc agctgcagag gtcaccgatc cgagcagtcg gcctctggtt ggtaaagacc
     4741 gtgcgactgc cggtgatgat cgtgttcaag ccatgcaggg ggcaggcgtg aaggtcatcc
     4801 tttcgggcca cggggcggct gttgacgaag gtgtcgtcgc tcccggtgat gatgatcccg
     4861 ccgtgatcgg tcacgtcgtt tagtcgagcg atgcctggcg tcgtagtcac gggtttaggt
     4921 caatacgact tgcggtcact gtaacgttgc cctcggcggt cacgttaacg tcgccttggg
     4981 cttcgacttg cgcctcctgc acaaggatca caatccgtcc ttgggctgcg gtgaggtcga
     5041 tcttgtactc atgcgcttcg cggtcgtact ggatgattga gtcatcctcg aactgcgtct
     5101 tttggatcgt ttctttgtcc tcgatctggg ggtagtcagt cgagaacgcg ccgggcatcg
     5161 cgaagccctg actgatctcg ccggaggggg ccatcacgac gacggcctca ccgacctcgg
     5221 gcgcccacca gaaccgatcc ttgcccgctc gctgcgtgag ccacggaatc cagtcagtga
     5281 ggagcagcgc ctcgccgctc tcctcgtctt cctcgatcgc gacacggatc agccccttgg
     5341 gatagtcagc ctcggctacc ctgcctacgc ggagcaagtt gccgtgacgc cgactgtctc
     5401 gagtatctag tcgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgt
//