LOCUS       PBAD-phzSM(wt)-B15@4A3  6978 bp ds-DNA     circular     09-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     BioBrick Prefix                3297..3318
                                    /label="BioBrick Prefix"
                                    /ApEinfo_revcolor=#b1ff67
                                    /ApEinfo_fwdcolor=#b1ff67
     Transcription Terminator       6829..6957
                                    /label="B15 terminator"
                                    /ApEinfo_revcolor=#b4abac
                                    /ApEinfo_fwdcolor=#b4abac
     misc_feature                   4517..4855
                                    /label="This section from PAO1 gDNA, downstream from UCBPP-PA14(syn)"
                                    /ApEinfo_revcolor=#c7b0e3
                                    /ApEinfo_fwdcolor=#c7b0e3
     misc_feature                   5769..6021
                                    /label="This section from PAO1 gDNA, downstream from UCBPP-PA14(syn)"
                                    /ApEinfo_revcolor=#c7b0e3
                                    /ApEinfo_fwdcolor=#c7b0e3
     gene                           complement(3319..4197)
                                    /label="araC"
                                    /ApEinfo_revcolor=#b1ff67
                                    /ApEinfo_fwdcolor=#b1ff67
     Promoter - arabinose inducible 4457..4508
                                    /label="PBAD"
                                    /ApEinfo_revcolor=#f58a5e
                                    /ApEinfo_fwdcolor=#f58a5e
     CDS                            complement(2202..2861)
                                    /label="AmpR"
                                    /ApEinfo_revcolor=#ffef86
                                    /ApEinfo_fwdcolor=#ffef86
     misc_feature                   4552..5760
                                    /label="phzS"
                                    /ApEinfo_revcolor=#f58a5e
                                    /ApEinfo_fwdcolor=#f58a5e
     replication gene               complement(363..1313)
                                    /label="repA (pSC101 origin)"
                                    /ApEinfo_revcolor=#b7e6d7
                                    /ApEinfo_fwdcolor=#b7e6d7
     BioBrick Suffix                complement(6958..6978)
                                    /label="BioBrick Suffix"
                                    /ApEinfo_revcolor=#f58a5e
                                    /ApEinfo_fwdcolor=#f58a5e
     misc_feature                   5816..6820
                                    /label="phzM"
                                    /ApEinfo_revcolor=#84b0dc
                                    /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tccggcaaaa aaacgggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
       61 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      121 cggcgagcgg tatcagctca ctcaaaggcg gtaatctcga ggttacattg tcgatctgtt
      181 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
      241 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
      301 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
      361 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
      421 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
      481 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
      541 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
      601 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
      661 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
      721 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
      781 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
      841 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
      901 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
      961 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     1021 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     1081 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     1141 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     1201 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     1261 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     1321 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     1381 catgtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
     1441 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     1501 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     1561 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     1621 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     1681 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     1741 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     1801 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     1861 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     1921 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     1981 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     2041 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     2101 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     2161 acaggagtcc aagcgagctc gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2221 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2281 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2341 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2401 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2461 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2521 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2581 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2641 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2701 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2761 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2821 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2881 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2941 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3001 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3061 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3121 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tcccatggtg
     3181 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     3241 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     3301 tcgcggccgc ttctagagTT ATGACAACTT GACGGCTACA TCATTCACTT TTTCTTCACA
     3361 ACCGGCACGG AACTCGCTCG GGCTGGCCCC GGTGCATTTT TTAAATACCC GCGAGAAATA
     3421 GAGTTGATCG TCAAAACCAA CATTGCGACC GACGGTGGCG ATAGGCATCC GGGTGGTGCT
     3481 CAAAAGCAGC TTCGCCTGGC TGATACGTTG GTCCTCGCGC CAGCTTAAGA CGCTAATCCC
     3541 TAACTGCTGG CGGAAAAGAT GTGACAGACG CGACGGCGAC AAGCAAACAT GCTGTGCGAC
     3601 GCTGGCGATA TCAAAATTGC TGTCTGCCAG GTGATCGCTG ATGTACTGAC AAGCCTCGCG
     3661 TACCCGATTA TCCATCGGTG GATGGAGCGA CTCGTTAATC GCTTCCATGC GCCGCAGTAA
     3721 CAATTGCTCA AGCAGATTTA TCGCCAGCAG CTCCGAATAG CGCCCTTCCC CTTGCCCGGC
     3781 GTTAATGATT TGCCCAAACA GGTCGCTGAA ATGCGGCTGG TGCGCTTCAT CCGGGCGAAA
     3841 GAACCCCGTA TTGGCAAATA TTGACGGCCA GTTAAGCCAT TCATGCCAGT AGGCGCGCGG
     3901 ACGAAAGTAA ACCCACTGGT GATACCATTC GCGAGCCTCC GGATGACGAC CGTAGTGATG
     3961 AATCTCTCCT GGCGGGAACA GCAAAATATC ACCCGGTCGG CAAACAAATT CTCGTCCCTG
     4021 ATTTTTCACC ACCCCCTGAC CGCGAATGGT GAGATTGAGA ATATAACCTT TCATTCCCAG
     4081 CGGTCGGTCG ATAAAAAAAT CGAGATAACC GTTGGCCTCA ATCGGCGTTA AACCCGCCAC
     4141 CAGATGGGCA TTAAACGAGT ATCCCGGCAG CAGGGGATCA TTTTGCGCTT CAGCCATACT
     4201 TTTCATACTC CCGCCATTCA GAGAAGAAAC CAATTGTCCA TATTGCATCA GACATTGCCG
     4261 TCACTGCGTC TTTTACTGGC TCTTCTCGCT AACCAAACCG GTAACCCCGC TTATTAAAAG
     4321 CATTCTGTAA CAAAGCGGGA CCAAAGCCAT GACAAAAACG CGTAACAAAA GTGTCTATAA
     4381 TCACGGCAGA AAAGTCCACA TTGATTATTT GCACGGCGTC ACACTTTGCT ATGCCATAGC
     4441 ATTTTTATCC ATAAGATTAG CGGATCCTAC CTGACGCTTT TTATCGCAAC TCTCTACTGT
     4501 TTCTCCATTA CTAGAGCCAT CGATTCGAAC ACTCGAGAAA AGGAAGCACC CATGAGCGAA
     4561 CCCATCGATA TCCTCATCGC CGGCGCCGGC ATCGGCGGCC TCAGTTGCGC CCTGGCCCTG
     4621 CACCAGGCCG GCATCGGCAA GGTCACGCTG CTGGAAAGCA GCAGCGAGAT ACGCCCCCTT
     4681 GGCGTCGGCA TCAATATCCA GCCGGCGGCG GTCGAGGCCC TTGCCGAACT GGGCCTCGGC
     4741 CCGGCGCTGG CGGCCACCGC CATCCCCACC CACGAGCTGC GCTACATCGA CCAGAGCGGC
     4801 GCCACGGTAT GGTCCGAGCC GCGCGGGGTG GAAGCCGGCA ACGCCTATCC GCAGTACTCG
     4861 ATCCATCGCG GCGAATTGCA GATGATCCTG CTCGCCGCGG TGCGCGAGCG TCTCGGCCAA
     4921 CAGGCGGTAC GCACCGGTCT CGGCGTGGAG CGCATCGAAG AGCGCGACGG CCGCGTACTG
     4981 ATCGGCGCCC GCGACGGACA CGGCAAGCCC CTGGCGCTCG GTGCCGATGT GCTGGTCGGC
     5041 GCCGACGGCA TCCATTCGGC GGTCCGCGCG CACCTGCATC CCGACCAGGG GCCGCTGTCC
     5101 CACGGTGGGA TCACCATGTG GCGCGGCGTC ACCGAGTTCG ACCGCTTCCT CGACGGCAAG
     5161 ACCATGATCG TCGCCAACGA CGAGCACTGG TCGCGCCTGG TCGCCTATCC GATCTCGGCG
     5221 CGCCACGCGG CCGAAGGCAA GTCGCTGGTG AACTGGGTGT GCATGGTGCC GAGCGCCGCC
     5281 GTCGGCCAGC TCGACAACGA GGCCGACTGG AACCGCAACG GACGCCTGGA AGACGTGTTG
     5341 CCGTTCTTCG CCGACTGGGA CCTGGGCTGG TTCGACATCC GCGACCTGCT GACCCGCAAC
     5401 CAGTTGATCT TGCAGTACCC GATGGTCGAC CGCGATCCGC TGCCGCACTG GGGCCGGGGA
     5461 CGCATCACGC TGCTCGGCGA CGCCGCCCAC CTGATGTATC CGATGGGCGC CAACGGCGCT
     5521 TCGCAGGCGA TCCTCGACGG CATCGAGCTG GCCGCCGCGC TGGCGCGCAA CGCCGACGTG
     5581 GCCGCAGCCC TGCGCGAATA CGAAGAAGCG CGGCGGCCGA CCGCCAACAA GATCATCCTG
     5641 GCCAACCGCG AACGGGAAAA AGAGGAATGG GCCGCGGCTT CGCGACCGAA GACTGAGAAG
     5701 AGCGCGGCGC TGGAAGCGAT CACCGGCAGC TACCGCAACC AGGTGGAACG GCCACGCTAA
     5761 tactagagTG GGTACTTCTC GGGTTACGGA AATGTGTAAC GAGAGAGAAT AAAAGATGAA
     5821 TAATTCGAAT CTTGCTGCTG CGCGTAATTT GATACAAGTT GTTACCGGGG AATGGAAGTC
     5881 CCGTTGCGTC TACGTCGCTA CGCGCCTCGG GCTGGCCGAT CTGATCGAGA GCGGGATCGA
     5941 CAGCGACGAG ACGCTGGCCG CCGCGGTCGG TTCCGATGCC GAGCGCATCC ATCGACTGAT
     6001 GCGCCTGCTG GTGGCCTTCG AGATCTTCCA GGGCGATACC CGCGACGGCT ACGCTAATAC
     6061 CCCCACCAGC CACCTGCTGA GGGATGTCGA GGGCTCCTTC CGCGACATGG TGCTGTTCTA
     6121 CGGCGAGGAG TTCCACGCCG CCTGGACGCC CGCCTGCGAG GCGCTGCTCA GCGGTACCCC
     6181 AGGCTTCGAA CTGGCGTTCG GCGAAGACTT CTACAGCTAC CTGAAGCGCT GCCCGGATGC
     6241 CGGCCGGCGC TTCCTGCTGG CGATGAAGGC GAGCAACCTG GCCTTCCACG AGATCCCCAG
     6301 GCTCCTGGAT TTCCGCGGGC GTAGCTTCGT CGACGTCGGT GGCGGTTCCG GCGAATTGAC
     6361 CAAGGCCATC CTGCAAGCCG AGCCCAGCGC CCGGGGCGTG ATGCTCGATC GCGAGGGTTC
     6421 CCTCGGCGTG GCCCGCGACA ACCTCTCCAG CCTGTTGGCA GGGGAGCGCG TCAGCCTGGT
     6481 GGGCGGCGAC ATGCTGCAAG AGGTGCCGTC CAACGGCGAT ATCTACCTGC TGTCGCGGAT
     6541 CATCGGCGAT CTGGACGAAG CCGCCAGCCT GAGGTTGCTC GGCAATTGCC GCGAGGCGAT
     6601 GGCCGGCGAC GGCCGGGTGG TGGTGATCGA GCGGACCATC TCGGCCAGCG AGCCGTCGCC
     6661 GATGTCGGTG CTCTGGGACG TGCACCTGTT CATGGCCTGC GCTGGCCGCC ACCGCACCAC
     6721 CGAGGAGGTG GTCGACCTGC TCGGGCGCGG CGGCTTCGCG GTGGAGCGGA TCGTCGACCT
     6781 GCCGATGGAA ACCCGCATGA TCGTCGCTGC CAGGGCCTAA tactagagcc aggcatcaaa
     6841 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6901 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatAC
     6961 TAGTagcggc cgctgcag
//