LOCUS       Exported                3507 bp ds-DNA     circular SYN 11-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(174..1034)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1035..1139)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1217..1672)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1658..1679)
                     /label=NM15SEP05
     primer_bind     complement(1660..1683)
                     /label=NM15OCT37
     primer_bind     1677..1708
                     /label=NM16FEB01
     primer_bind     1677..1705
                     /label=NM15DEC05
     primer_bind     1680..1709
                     /label=NM15OCT31
     primer_bind     1684..1705
                     /label= M16OCT08
     terminator      complement(join(1698..1725,1751..1775))
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1762..1778)
                     /label=NM15DEC06
     primer_bind     1775..1799
                     /label=NM15DEC07
     misc_feature    1776..1865
                     /label=spy Term
     primer_bind     complement(1841..1865)
                     /label=NM16FEB04
     primer_bind     complement(1842..1866)
                     /label=NM16AUG01
     primer_bind     complement(1842..1865)
                     /label=NM15DEC08
     primer_bind     1867..1883
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1884..1910)
                     /label=NM16OCT09
     promoter        1890..1908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1947..1967
                     /label=NM19MAR18
     misc_feature    1956..1958
                     /label=Kozak
     primer_bind     1968..1994
                     /label=ASP14NOV42
     promoter        complement(1969..2423)
                     /label=Hsp70B
     primer_bind     1969..1994
                     /label=NM16NOV21
     primer_bind     complement(2293..2314)
                     /label=ASP14NOV41
     primer_bind     2310..2335
                     /label=ASP14NOV40
     primer_bind     complement(2402..2427)
                     /label=NM19MAR17
     primer_bind     complement(2402..2427)
                     /label=NM17FEB01
     primer_bind     complement(2402..2423)
                     /label=ASP14NOV39
     primer_bind     complement(2403..2423)
                     /label=NM18AUG01
     primer_bind     2423..2448
                     /label=NM19MAR15
     promoter        2424..2503
                     /label=3xP3 eye promoter
     misc_feature    2456..2468
                     /label=P3 enhancer
     misc_feature    2471..2483
                     /label=P3 enhancer
     misc_feature    2487..2499
                     /label=RCSI/P3 enhancer
     primer_bind     complement(2493..2512)
                     /label=NM19MAR16
     primer_bind     2503..2534
                     /label=NM15MAY01
     primer_bind     2508..2534
                     /label=NM15MAY02
     primer_bind     2544..2572
                     /label=NM16OCT10
     promoter        complement(2544..2562)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2578..2594)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2594..2615
                     /label=NM16AUG02
     primer_bind     2596..2618
                     /label=NM15DEC09
     primer_bind     2596..2618
                     /label=NM16FEB03
     misc_feature    complement(2597..2657)
                     /label=L3S2P21 Term
                     /note="Synthetic Terminator. Very Strong. See Chen, 2013 
                     (Chris Voigt)"
     primer_bind     complement(2632..2657)
                     /label=NM15DEC10
     primer_bind     2658..2682
                     /label=NM15DEC11
     misc_feature    2667..2711
                     /label= rrnB T1 Terminator
     primer_bind     complement(2711..2748)
                     /label=NM16FEB02
     primer_bind     complement(2711..2736)
                     /label=NM15DEC12
     primer_bind     complement(2721..2736)
                     /label=NM16OCT11
     primer_bind     complement(2724..2740)
                     /label=NM15OCT32
     primer_bind     2734..2763
                     /label=NM15OCT38
     primer_bind     2734..2763
                     /label=NM15SEP06
     rep_origin      complement(join(2922..3507,1..3))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
       61 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      181 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      241 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      301 gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      361 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      421 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      481 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      541 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      601 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      661 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
      721 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
      781 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      841 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      901 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      961 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1021 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1141 acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag
     1201 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     1261 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     1321 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     1381 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcgc tggcccacta
     1441 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     1501 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     1561 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     1621 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgt
     1681 aacattgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     1741 tggcagttta tggcgggcgt cctgcccgcc accctttcag ccaaaaaact taagaccgcc
     1801 ggtcttgtcc actaccttgc agtaatgcgg tggacaggat cggcggtttt cttttctctt
     1861 ctcaatgtaa aacgacggcc agtgaattgt aatacgactc actatagggt gaattccgct
     1921 taggacctta gatattgatc tcaaggtctc gcattttgtt ttcctccttt gagagttctc
     1981 ttcttgtatt caataattac ttcttggcag atttcagtag ttgcagttga tttacttggt
     2041 tgctggttac ttttaattga ttcactttaa cttgcacttt actgcagatt gtttagcttg
     2101 ttcagctgcg cttgtttatt tgcttagctt tcgcttagcg acgtgttcac tttgcttgtt
     2161 tgaattgaat tgtcgctccg tagacgaagc gcctctattt atactccggc gctcttttcg
     2221 cgaacattcg aggcgcgctc tctcgaacca acgagagcag tatgccgttt actgtgtgac
     2281 agagtgagag agcattagtg cagagaggga gcgacccaaa aagaaaagag agaataacga
     2341 ataacggcca gagaaatttc tcgagttttc tttctgccaa acaaatgacc taccacaata
     2401 accagtttgt tttgggattc taggggagag ggttcgaaat cgataagctt ggatcctaat
     2461 tgaattagct ctaattgaat tagtctctaa ttgaattaga tccctcccga gaccactcat
     2521 cgcccaatca ctagtgaatt cgttctatag tgtcacctaa ataccataga gccgttggtc
     2581 atagctgttt cctgtcggac caaaacgaaa aaaggccccc ctttcgggag gcctcttttc
     2641 tggaatttgg taccgagcag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2701 tttcgtttta tctgttgttt gtcggtgaac gctctctgac cttccgcttc ctcgctcact
     2761 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3481 cagattacgc gcagaaaaaa aggatct
//
