LOCUS       Exported                3218 bp ds-DNA     circular SYN 07-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Alejandro Sarrion-Perdigones
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        530..634
                     /gene="bla"
                     /label=AmpR promoter
     CDS             635..1495
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1666..2254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2542..2563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2578..2608
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2616..2632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2640..2656
                     /label=M13R
     promoter        2674..2692
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2675..2691
                     /label=Sp6
     promoter        2795..2949
                     /label=DSCP
     primer_bind     2795..2810
                     /label=NM17JAN03
     primer_bind     2795..2810
                     /label=NM16NOV19
     TATA_signal     2823..2828
                     /label=TATA
     misc_feature    2852..2857
                     /label=Inr
                     /note="Inititator"
     misc_feature    2871..2880
                     /label=MTE
                     /note="Motif Ten Element"
     misc_feature    2881..2886
                     /label=DPE
                     /note="Downstream Promoter Element"
     primer_bind     complement(2938..2960)
                     /label=NM16NOV20
     primer_bind     complement(3031..3051)
                     /label=T7
     promoter        complement(3033..3051)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3058..3075)
                     /label=M13F(-21)
     primer_bind     complement(3078..3095)
                     /label=M13F(-41)
     rep_origin      join(3215..3218,1..452)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
       61 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      121 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      181 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccagc
      241 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      301 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      361 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
      421 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
      481 ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      541 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      601 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      661 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      721 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      781 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      841 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      901 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      961 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1021 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1081 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1141 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1201 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1261 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1321 tgctgataaa tctggagccg gtgagcgtgg gtcgcgcggt atcattgcag cactggggcc
     1381 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1441 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1501 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2401 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2461 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2521 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2581 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2641 aggaaacagc tatgaccatg attacgccaa gctatttagg tgacactata gaatactcaa
     2701 gctatgcatc caacgcgttg ggagctctcc catatggtcg acctgcaggc ggccgcgaat
     2761 tcactagtga ttgggcgatg agtggtctcg tcccgagctc gcccggggat cgagcgcagc
     2821 ggtataaaag ggcgcggggt ggctgagagc atcagttgtg aatgaatgtt cgagccgagc
     2881 agacgtgccg ctgccttcgt taatatcctt tgaataagcc aactttgaat cacaagacgc
     2941 ataccaaacc aaatgcgaga ccactcatcg cccaatcgaa ttcccgcggc cgccatggcg
     3001 gccgggagca tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt acaattcact
     3061 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3121 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3181 ttcccaacag ttgcgcagcc tgaatggcga atggacgc
//
