LOCUS       Exported                5459 bp ds-DNA     circular SYN 27-JUL-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Alejandro Sarrion-Perdigones
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     385..401
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        408..426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     500..528
                     /label=ASP14NOV05
     promoter        508..2906
                     /label=alphaTub84B
     primer_bind     1777..1798
                     /label=ASP15JUL58
     primer_bind     complement(2051..2071)
                     /label=ASP14NOV06
     primer_bind     2067..2091
                     /label=ASP14NOV07
     primer_bind     complement(2632..2652)
                     /label=ASP14NOV08
     primer_bind     2649..2672
                     /label=ASP14NOV09
     primer_bind     complement(2704..2727)
                     /label=ASP14NOV10
     primer_bind     2724..2746
                     /label=ASP14NOV11
     primer_bind     complement(2883..2913)
                     /label=ASP14NOV12
     promoter        complement(3008..3026)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3044..3060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3068..3084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3092..3122)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3137..3158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3446..4034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4205..5065)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5066..5170)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(5248..5459,1..244))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
       61 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
      121 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
      181 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
      241 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      301 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      361 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
      421 tatagggcga attgggcccg acgtcgcatg ctcccggccg ccatggcggc cgcgggaatt
      481 cgattgggcg atgagtggtc tcgggagaaa acgaacgtat tgcttaggga cacagctcac
      541 attaataaga cgccagtcct cgctgtcgca gaagaacttc ctcatagccc gcagcttggc
      601 gacggtagcg ttggaagctc gatgctcgaa cgtgtgaaat gtgttgcctg ccaattcaga
      661 agccaggtga aattggtagg agctcaatgc agtactaacc cgacttaccg atgtcgacga
      721 agaggtccag gtccgaggat cgatttccaa tgcctgtgat gcgggacccg aacttgtaga
      781 ctcgcagtgg ttgtttggga aagcccggcg acaaaagctt gcacaggtcc tgttcgataa
      841 cgtcgtactc gggaatcacc tttaacttgt ccgctattgg gtatttgtcc ttaaacacat
      901 tcgtaatctc tcccacaatc attgccctgg cctctgcaat gtaaaaagta tttataaaat
      961 atcgctggct ctaaagaaaa catattaatc aaagtattga ggctttatca atctaacatt
     1021 tacaagtagt cagaccacaa tttttagact gcacttctgc gtaagtaaat gaaatgaaat
     1081 ggtgacctac tcttgggtag ccgtactgtt atatgctgac gagccttcac ctgggcctgc
     1141 tttttctcct gtttcgtctg atgcgtcgga tgcggctgga ctggctgatt gttgggattg
     1201 ggttttggat gctgctccga aaggtgggcc acagcttttg cgatggtggt gcccactatg
     1261 cggttgctgc aggaactaca gctgaatgtt cgctttattg tctgcagctc ctggggctgg
     1321 acgaaggcca cgccctcacc cggtgcggcc atctcctgcg gcaaaacgta tgtgggattc
     1381 ctgtccaagt agctctcgat gcccaccata atgcgctcca gcaggttggc caggtgcggg
     1441 tctagctgcg gcggattcgt cgtctggttc actgagttta gggtctgcag aaagaagtgt
     1501 gcctccatgg taagcggatt catctgcacg atcagcgggc tttagtctaa aaattcgaat
     1561 gatacaaact cgtactcacg taaagcagtt tgtttggcat catctcggcc atcgtcttat
     1621 tcttcttcgt ggcctttgtt accggagcgg ctatgttggg cgcggtggag cccggaagct
     1681 gaaaggttaa tcgaatgcgt taacttttct gcaactcgaa agtttgccgc ctttgttcga
     1741 ctgccaataa ctgttgattc gaaaattcga atcgaagcgg ttgaatttcg tagggtggcc
     1801 aactacacca aagttcgccg gcggtgtatg catgtgagtg tgagtgtgac agtgggtgta
     1861 cgtgtgcttt aagcgacaca atcgcaccgc gatgtacata tttttgtaga aatgtgtgtg
     1921 catttttcgt ccggatcacg gctccataaa ataaatcata tcacagggct cagggatttg
     1981 tgagtagaag ttcccgccac ttaaacgtca cttgtgaaat cgccatcgac ggtcaacttt
     2041 tactcacact ggtcttccgc ggacggacag tctcaaagta ctgcctttct gcgttggaaa
     2101 acatcgcctt tttcgtccaa aaggagtccc caggttcgat ccgcatggcg ttgtgcgtgc
     2161 gtgcctttct tttcaaatga ttacggctat taacttgggg gcgttaagtt ggaaacacgt
     2221 aaattgcaga ctgcgattag agtgaccatg agtaggagtt caaaatctcc tgacatcatt
     2281 ttcttaaaac ctgctttgtt ttttacattt ctatttaata taactcctat ttgaataaaa
     2341 aaacaaaaca agtttagatg ttaagatatt aactacatcc tttgctccaa agggagaggg
     2401 gaagttatgg agttaattaa tttgctgttg gaaatcaata tggagtcaga aatataatga
     2461 tttactaaac cttattgaat cggtaacgat gcgaatttat attaaaatag cttttatgaa
     2521 acattcaaca aaaatattat taatgttggc ccactttagc aaccggttag gtctaccggt
     2581 tgggcaagca aagattcacg ccctggttcg agtcccaact agtcctgcaa aataccgcag
     2641 caagttttag atagaccaag tgccattacc tctcccactt cagttatcgg ttatgcggcg
     2701 tttaagtcga cagcttgccg tctatagctc cggtgcctat ataaagcagc ccgctttcca
     2761 catttcatat tcgttttacg tttgtcaagc ctcatagccg gcagttcgaa cgtatacgct
     2821 ctctgagtca gacctcgaaa tcgtagctct acacaattct gtgaattttc cttgtcgcgt
     2881 gtgaaacact tccaataaaa actcaaatgc gagaccactc atcgcccaat cactagtgaa
     2941 ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct
     3001 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
     3061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4321 gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa
     4381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5161 gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga
     5221 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     5281 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5341 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5401 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggc
//
