LOCUS       Exported                5068 bp ds-DNA     circular SYN 19-MAR-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        complement(99..117)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(100..116)
                     /label=Sp6
     primer_bind     complement(135..151)
                     /label=M13R
     protein_bind    159..175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(183..213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(537..1125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1296..2156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2157..2261)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2339..2794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2914..2931
                     /label=M13F(-41)
     primer_bind     2934..2951
                     /label=M13F(-21)
     primer_bind     2958..2978
                     /label=T7
     promoter        2958..2976
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3050..3077
                     /label=ASP14NOV01
     promoter        3058..5065
                     /label=Polyubiquitin Promoter
     primer_bind     3750..3773
                     /label=ASP14NOV107
     primer_bind     complement(3903..3926)
                     /label=ASP14NOV02
     primer_bind     3921..3946
                     /label=ASP14NOV03
     primer_bind     complement(join(5046..5068,1..4))
                     /label=ASP14NOV04
ORIGIN
        1 cgagaccact catcgcccaa tcactagtga attcgcggcc gcctgcaggt cgaccatatg
       61 ggagagctcc caacgcgttg gatgcatagc ttgagtattc tatagtgtca cctaaatagc
      121 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      181 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      241 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      301 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      361 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      421 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      481 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      541 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      601 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      661 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      721 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      781 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      841 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      961 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1021 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1081 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1141 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1201 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1261 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1321 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1381 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac
     1441 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1501 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1561 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1621 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1681 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1741 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1801 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1861 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1921 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1981 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2041 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2101 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2161 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2221 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2281 ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
     2341 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     2401 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     2461 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     2521 gtcaaagggc gaaaaaccgt ctatcagggc gctggcccac tacgtgaacc atcaccctaa
     2581 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     2641 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     2701 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     2761 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact
     2821 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     2881 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     2941 cgacggccag tgaattgtaa tacgactcac tatagggcga attgggcccg acgtcgcatg
     3001 ctcccggccg ccatggcggc cgcgggaatt cgattgggcg atgagtggtc tcgggagaca
     3061 tagatcttgt cgccggaacg cagcgacagg gattccaatg tgtccgtatc ctttaggctt
     3121 ttgcccttca gttccagacg aagcgactgg cgattcgcgt gtggggtctg cttcagggtc
     3181 ttgtgaatta gggcgcgcag atcgccgatg ggcgtggcgc cggagggcac cttcaccttg
     3241 ccgtacggct tgctgttctt cgcgttcaaa atctccagct ccattttgct ttcggtgcgc
     3301 ttgcaatcag tactgtccaa aatcgaaaat cgccgaaccg tagtgtgacc gtgcggggct
     3361 ctgcgaaaat aaactttttt aggtatatgg ccacacacgg ggaaagcaca gtggattata
     3421 tgtattaata tattatccag gttttcatta cttatccaga tgtaagccca cttaaagcga
     3481 tttaacaatt atttgccgaa agagtataaa caaattttac ttaaaaatgg attaagaaaa
     3541 gcttgtgtaa gattatgcgc agcgttgcca gatagctcca tttaaaacac ttcaaaaaca
     3601 ataagttttg aaaatatata cataaatagc agtcgttgcc gcaacgctca acacatcaca
     3661 cttttaaaac accctttacc tacacagaat tactttttaa atttccagtc aagctgcgag
     3721 tttcaaaatt atagccggta gagaagacag tgctatttca aaagcaaact aacaagggtc
     3781 ttaaattcca aaacaccaat cctaacaagc cttggacttt tgtaagttta gatcaaaggt
     3841 ggcattgcat tcaatgtcat ggtaagaagt aggtcgtcta ggtagaaatc ctcattcagc
     3901 cggtcaagtc agtacgagaa aggtgtcaat ttgaaattgt cttaaaaata ttttattgtt
     3961 ttgtactgtg gtgagtttaa acgaaaaaca caaaaaaaaa gtgatacata gaaatcataa
     4021 aaaattttaa tacaaggtat tcgtacgtat caaaaacatt tcggcacaat tttttttctc
     4081 tgtactaaag tgttacgaac actacggtat tttttagtga ttttcaacgg acaccgaagg
     4141 tatataaaca gcgttcgcga acggtcgcct tcaaaaccaa ttgacatttg cagcagcaag
     4201 tacaagcaga aagtaaagcg caatcagcga aaaatttata cttaattgtt ggtgattaaa
     4261 gtacaattaa aagaacattc tcgaaagtca caagaaacgt aagtttttaa ctcgctgtta
     4321 ccaattagta ataagagcaa caagacgttg agtaatttca agaaaaactg catttcaagg
     4381 tctttgttcg gccatttttt tttattcaac gctctacgta attacaaaat aagaaattgg
     4441 cagccacgca tctcgttttc ccaatgaatt ggcatcaaaa cgcaaacaaa tctataaata
     4501 aaacttacgt gttgattttc gccaagattt attggcaaat tgtgaaattc gcagtgacgt
     4561 atttgaaaat tcgagaaatc acatacgcac tcgagcattt gtgtgcatgt tattagttag
     4621 ttgtttagtt aattgaagta ttttaccaac gaaatccact tatttttagc tgaaatagag
     4681 taggttgctt aaacaaagcc acgtctgaaa atttcttatt gcttgtagtt gtgacgtcac
     4741 catatacaca caaaataatg tgtatgcatg catttcagct gtgtatacat acatgcacac
     4801 actcgcaaca cgaaaacgat gacgaagcaa cggaacaaag gtttctcaac taccctttgt
     4861 tccctgtttc ttcgctttcc tttgttccaa tattcgtaga gggttaatag gggtttctca
     4921 acaaagttgg cgtcgataaa taagtttccc atttttattc cccagccaga aagttagttt
     4981 caatagtttt gtaatttcaa cgaaactcgt ctgatttcgt actaattttc cacatctcta
     5041 ttttcttccc gcagaataat ccaaaatg
//
