LOCUS       Exported                3727 bp ds-DNA     circular SYN 11-MAR-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Alejandro Sarrion-Perdigones
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     385..401
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        408..426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     500..527
                     /label=ASP15MAR02
     terminator      508..1173
                     /label=P10
     primer_bind     508..527
                     /label=ASP15MAR03
     primer_bind     508..527
                     /label=ASP14NOV18
     primer_bind     complement(1153..1181)
                     /label=ASP15MAR01
     primer_bind     complement(1153..1181)
                     /label=ASP14NOV17
     promoter        complement(1276..1294)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1312..1328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1336..1352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1360..1390)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1405..1426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1714..2302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2473..3333)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3334..3438)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(3516..3727,1..244))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
       61 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
      121 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
      181 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
      241 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      301 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      361 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
      421 tatagggcga attgggcccg acgtcgcatg ctcccggccg ccatggcggc cgcgggaatt
      481 cgattgggcg atgagtggtc tcgcattgtt aactcgaatc gctatccaag ccagctccat
      541 tgtcggcatc gtgctcattc tattgacgct ggcagatttg gttttggcgc tatgggaccc
      601 gttcggttac aacaacatgt ttccgcgcga gtttcccgac gacatgtcgc gcacgttcct
      661 gactgcgtac tttgagagtt tcgacaacac cacgtccaga gaaatcatag agtttatgcc
      721 cgagttcttt tcggaaatgg tcgaaacgga cgatgacgcc acgtttgaat ctctatttca
      781 tttattagat tatgtggcat ctttagaagt taattccgac ggccaaatgt taaacttgga
      841 ggagggtgat gaaattgagg attttgacga atctactttg gtggggcaag cgttagccac
      901 tagctcgcta tacactcgca tggagtttat gcagtacacg tttaggcaaa acacactatt
      961 gtctatgaac aaagaaaaca acaattttaa tcaaataatc atgggtttat ttgcaacaaa
     1021 cacaattgtg gcgtttacag catttgttat acacacagaa ctcatatttt ttatattttt
     1081 cgtaatcttc ctaatgatca cattttatta cataatcaaa gaatcgtacg aatattataa
     1141 aacaattgat ttgttatttt aaaaacgatt catctcccga gaccactcat cgcccaatca
     1201 ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat
     1261 gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct
     1321 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1381 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1441 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1501 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1561 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1621 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2221 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2281 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2341 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2401 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2461 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2521 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2581 accatctggc cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt
     2641 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2701 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2761 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2821 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2881 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2941 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3001 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3061 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3121 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3181 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3241 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3301 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3361 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3421 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac
     3481 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt
     3541 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3601 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3661 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3721 tcagggc
//
