LOCUS       Exported                3040 bp ds-DNA     circular SYN 07-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(186..774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1805)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1806..1910)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1988..2443)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2429..2450)
                     /label=NM15SEP05
     primer_bind     complement(2431..2454)
                     /label=NM15OCT37
     primer_bind     2448..2476
                     /label=NM15DEC05
     primer_bind     2451..2480
                     /label=NM15OCT31
     terminator      complement(join(2469..2496,2522..2546))
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2533..2549)
                     /label=NM15DEC06
     primer_bind     2546..2570
                     /label=NM15DEC07
     misc_feature    2547..2636
                     /label=spy Term
     primer_bind     complement(2613..2636)
                     /label=NM15DEC08
     primer_bind     2638..2654
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2661..2679
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2719..2744
                     /label=NM15OCT02
     primer_bind     2719..2738
                     /label=NM16NOV21
     mRNA            complement(2734..2739)
                     /label=SD
     promoter        complement(2740..2807)
                     /label=CP6 Promoter
     primer_bind     complement(2787..2815)
                     /label=NM15DEC18
     primer_bind     complement(2787..2807)
                     /label=ASP14SEP09
     primer_bind     complement(2792..2807)
                     /label=NM19JUN07
     primer_bind     2812..2838
                     /label=NM15MAY02
     primer_bind     2812..2838
                     /label=NM15MAY01
     promoter        complement(2848..2866)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2882..2898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2900..2922
                     /label=NM15DEC09
     misc_feature    complement(2901..2961)
                     /label=L3S2P21 Term
                     /note="Synthetic Terminator. Very Strong. See Chen, 2013 
                     (Chris Voigt)"
     primer_bind     complement(2936..2961)
                     /label=NM15DEC10
     primer_bind     2962..2986
                     /label=NM15DEC11
     misc_feature    2971..3015
                     /label= rrnB T1 Terminator
     primer_bind     complement(3015..3040)
                     /label=NM15DEC12
     primer_bind     complement(join(3028..3040,1..4))
                     /label=NM15OCT32
     primer_bind     join(3038..3040,1..27)
                     /label=NM15OCT38
     primer_bind     join(3038..3040,1..27)
                     /label=NM15SEP06
ORIGIN
        1 tgaccttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
       61 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      601 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
      961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1081 ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1921 cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1981 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     2041 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     2101 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     2161 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact acgtgaacca
     2221 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     2281 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     2341 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     2401 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg taacattgta
     2461 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt
     2521 atggcgggcg tcctgcccgc caccctttca gccaaaaaac ttaagaccgc cggtcttgtc
     2581 cactaccttg cagtaatgcg gtggacagga tcggcggttt tcttttctct tctcaatgta
     2641 aaacgacggc cagtgaattg taatacgact cactataggg tgaattccgc ttaggacctt
     2701 agatattgat ctcaaggtct cgcattttgt tttcctcctt atgttaagct tactcagtta
     2761 ttatatcata aatatctgtg tcaagaataa actcccacat ggattcgctc ccgagaccac
     2821 tcatcgccca atcactagtg aattcgttct atagtgtcac ctaaatacca tagagccgtt
     2881 ggtcatagct gtttcctgtc ggaccaaaac gaaaaaaggc ccccctttcg ggaggcctct
     2941 tttctggaat ttggtaccga gcaggcatca aataaaacga aaggctcagt cgaaagactg
     3001 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
//
