LOCUS       Exported                2988 bp ds-DNA     circular SYN 25-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             complement(174..1034)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1035..1139)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1217..1672)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1658..1679)
                     /label=NM15SEP05
     primer_bind     complement(1660..1683)
                     /label=NM15OCT37
     primer_bind     1677..1708
                     /label=NM16FEB01
     primer_bind     1677..1705
                     /label=NM15DEC05
     primer_bind     1680..1709
                     /label=NM15OCT31
     primer_bind     1684..1705
                     /label= M16OCT08
     terminator      complement(join(1698..1725,1751..1775))
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1762..1778)
                     /label=NM15DEC06
     primer_bind     1775..1799
                     /label=NM15DEC07
     misc_feature    1776..1865
                     /label=spy Term
     primer_bind     complement(1841..1865)
                     /label=NM16FEB04
     primer_bind     complement(1842..1866)
                     /label=NM16AUG01
     primer_bind     complement(1842..1865)
                     /label=NM15DEC08
     primer_bind     1867..1883
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1884..1910)
                     /label=NM16OCT09
     promoter        1890..1908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1948..2015
                     /label=NM15MAY02
     CDS             1952..1987
                     /codon_start=1
                     /label=GGS4 TL2
                     /translation="GGSGGSGGSGGS"
     primer_bind     1989..2015
                     /label=NM15MAY01
     primer_bind     2025..2053
                     /label=NM16OCT10
     promoter        complement(2025..2043)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2059..2075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2075..2096
                     /label=NM16AUG02
     primer_bind     2077..2099
                     /label=NM15DEC09
     primer_bind     2077..2099
                     /label=NM16FEB03
     misc_feature    complement(2078..2138)
                     /label=L3S2P21 Term
                     /note="Synthetic Terminator. Very Strong. See Chen, 2013 
                     (Chris Voigt)"
     primer_bind     complement(2113..2138)
                     /label=NM15DEC10
     primer_bind     2139..2163
                     /label=NM15DEC11
     misc_feature    2148..2192
                     /label= rrnB T1 Terminator
     primer_bind     complement(2192..2229)
                     /label=NM16FEB02
     primer_bind     complement(2192..2217)
                     /label=NM15DEC12
     primer_bind     complement(2202..2217)
                     /label=NM16OCT11
     primer_bind     complement(2205..2221)
                     /label=NM15OCT32
     primer_bind     2215..2244
                     /label=NM15OCT38
     primer_bind     2215..2244
                     /label=NM15SEP06
     rep_origin      complement(join(2403..2988,1..3))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
       61 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      181 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      241 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      301 gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      361 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      421 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      481 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      541 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      601 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      661 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
      721 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
      781 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      841 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      901 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      961 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1021 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1141 acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag
     1201 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     1261 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     1321 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     1381 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcgc tggcccacta
     1441 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     1501 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     1561 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     1621 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgt
     1681 aacattgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     1741 tggcagttta tggcgggcgt cctgcccgcc accctttcag ccaaaaaact taagaccgcc
     1801 ggtcttgtcc actaccttgc agtaatgcgg tggacaggat cggcggtttt cttttctctt
     1861 ctcaatgtaa aacgacggcc agtgaattgt aatacgactc actatagggt gaattccgct
     1921 taggacctta gatattgatc tcaaggtctc gggaggttcc ggtggaagcg gaggtagcgg
     1981 cggcagtgcg agaccactca tcgcccaatc actagtgaat tcgttctata gtgtcaccta
     2041 aataccatag agccgttggt catagctgtt tcctgtcgga ccaaaacgaa aaaaggcccc
     2101 cctttcggga ggcctctttt ctggaatttg gtaccgagca ggcatcaaat aaaacgaaag
     2161 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctga
     2221 ccttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2281 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2341 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2401 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2461 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2521 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2581 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2641 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2701 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2761 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2821 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2881 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2941 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatct
//
