LOCUS       Exported                2851 bp ds-DNA     circular SYN 24-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     1..32
                     /label=NM15OCT29
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     100..118
                     /label=NM19JUN06
     CDS             complement(109..615)
                     /codon_start=1
                     /label=QFAD
                     /translation="SRQLELPPTATATASIMPHVMEQPLSTSINPVNDRFNGIPNPTPY
                     NSDAALDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYVDSTT
                     NHPPYHSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCNFSDI
                     NTYEQ"
     primer_bind     complement(604..621)
                     /label=NM19JUN05
     primer_bind     617..643
                     /label=NM15MAY02
     primer_bind     617..643
                     /label=NM15MAY01
     promoter        complement(653..671)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(687..703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(701..718)
                     /label=NM15OCT30
     primer_bind     715..738
                     /label=ASP14NOV50
     rep_origin      complement(800..1482)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(1495..1520)
                     /label=ASP14NOV51
     primer_bind     1517..1541
                     /label=ASP14JUL17
     primer_bind     1520..1541
                     /label=ASP15JUN29
     promoter        1521..1619
                     /label=cat Promoter
     CDS             1624..2280
                     /codon_start=1
                     /label=Chloramphenicol Resistance
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2041..2060)
                     /label=ASP14JUL18
     primer_bind     2057..2080
                     /label=ASP14JUL19
     terminator      2284..2391
     primer_bind     complement(2372..2395)
                     /label=ASP14JUL20
     primer_bind     2392..2415
                     /label=ASP15JAN01
     rep_origin      2396..2851
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(join(2820..2851,1..4))
                     /label=ASP15JUL28
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaagctt actgctcgta
      121 agtgttgatg tcggagaagt tgcagccggg aacgaaaccc aggttggtca tgaactgcgg
      181 atcgacgtca acggcgtcag tgtactcgat ggaagccaaa tcgtccacca tggcatcgta
      241 gtcaacggtg gagtatccga agttagccat gggcaccagg ttggagtggt agggcgggtg
      301 gttggtggtg gagtcgacgt aggggggcgg gggacggggt ggagctccgc cgggagccag
      361 ggcgacggag gcctcgttga ggtgggtggc tggcgggggg taagtggaca agatgccgtg
      421 ggtattgacg gaaccgtagt cgttggtctg ggtgatggcg tccagggcgg cgtcggagtt
      481 gtagggggtg gggtttggga tgccgttgaa gcgatcgttg acggggttga tggaggtgga
      541 cagcggctgc tccatgacgt gtggcataat cgaagcggtg gcggtggcag tgggtggcag
      601 ctccagctgg cgcgagcgag accactcatc gcccaatcac tagtgaattc gttctatagt
      661 gtcacctaaa taccatagag ccgttggtca tagctgtttc ctgtccaggc atcatgacac
      721 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      781 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      841 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      901 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      961 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1021 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1081 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1141 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1201 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1261 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1321 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1381 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1441 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1501 tcacctagat ccttttgtga tgatcggcac gtaagaggtt ccaactttca ccataatgaa
     1561 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
     1621 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1681 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1741 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1801 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     1861 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1921 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1981 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     2041 gagaatatgt ttttcgtgtc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     2101 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     2161 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     2221 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     2281 taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt
     2341 gataataagc ggatgaatgg cagaaattcg atgataagct gtcaaacatg atgttacgcg
     2401 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2461 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2521 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2581 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccagcg
     2641 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2701 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2761 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2821 aattttaaca aaatattaac gcttacaatt t
//
