LOCUS       Exported                3777 bp ds-DNA     circular SYN 14-JAN-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Alejandro Sarrion-Perdigones
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(99..115)
                     /label=Sp6
     primer_bind     complement(134..150)
                     /label=M13R
     protein_bind    158..174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(182..212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    227..248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(536..1124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1295..2155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2156..2260)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2338..2793)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2913..2930
                     /label=M13F(-41)
     primer_bind     2933..2950
                     /label=M13F(-21)
     primer_bind     2957..2977
                     /label=T7
     primer_bind     3049..3072
                     /label=ASP14OCT58
     CDS             3054..3770
                     /codon_start=1
                     /product="Codon optimized DM"
                     /label=EBFP2
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFNDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(join(3751..3777,1..4))
                     /label=ASP14OCT57
ORIGIN
        1 cgagaccact catcgcccat cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg
       61 gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct
      121 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc
     1441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     2101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     2161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2281 cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     2341 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     2401 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     2461 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     2521 tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact acgtgaacca tcaccctaat
     2581 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     2641 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     2701 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     2761 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     2821 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     2881 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     2941 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggcccga cgtcgcatgc
     3001 tcccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgaatggtgt
     3061 ccaagggcga ggagctgttc accggcgtgg tgcccatcct ggtggagctg gatggcgacg
     3121 tgaacggcca caagttcagc gtgcgcggcg agggcgaggg cgacgccacc aacggcaagc
     3181 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctggtga
     3241 ccaccctgag ccacggcgtg cagtgcttcg cccgctaccc cgatcacatg aagcagcacg
     3301 atttcttcaa gagcgccatg cccgagggct acgtgcagga gcgcaccatt ttcttcaatg
     3361 acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgatacc ctggtgaacc
     3421 gcatcgagct gaagggcgtg gatttcaagg aggatggcaa catcctgggc cacaagctgg
     3481 agtacaactt caacagccac aacatctaca tcatggccgt gaagcagaag aacggcatca
     3541 aggtcaactt caagatccgc cacaacgtgg aggatggctc agtgcagctg gccgatcact
     3601 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgatagc cactacctga
     3661 gcacccagag cgtgctgtcc aaggatccca acgagaagcg cgatcacatg gtgctgctgg
     3721 agttccgcac cgccgccggc atcaccctgg gcatggatga gctgtacaag taagctt
//
