LOCUS       Exported                3766 bp ds-DNA     circular SYN 10-FEB-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Alejandro Sarrion-Perdigones
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     385..401
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        408..426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     500..525
                     /label=ASP14OCT56
     CDS             505..1212
                     /codon_start=1
                     /product="Codon optimized for DM"
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     505..525
                     /label=ASP15FEB25
     primer_bind     complement(1193..1220)
                     /label=ASP15FEB26
     primer_bind     complement(1193..1212)
                     /label=ASP14OCT57
     promoter        complement(1315..1333)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1351..1367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1375..1391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1399..1429)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1444..1465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1753..2341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2512..3372)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3373..3477)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(3555..3766,1..244))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
       61 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
      121 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
      181 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
      241 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      301 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      361 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
      421 tatagggcga attgggcccg acgtcgcatg ctcccggccg ccatggcggc cgcgggaatt
      481 cgattgggcg atgagtggtc tcgaatggtg tccaagggag aagaggataa catggccatc
      541 atcaaggagt tcatgcgctt caaggtccac atggagggca gcgtgaatgg ccacgagttc
      601 gagatcgagg gcgaaggcga gggacgtccc tacgaaggca cccagaccgc caaactgaag
      661 gtgaccaagg gaggcccgtt gccctttgcc tgggacatct tgagccccca gttcatgtac
      721 ggctccaagg cctacgtgaa gcacccggcc gacattcccg actacctgaa gctgagcttc
      781 cccgaaggct tcaagtggga gcgcgtgatg aacttcgagg acggcggagt ggtgaccgtg
      841 acccaggact ccagcttgca ggacggcgag ttcatctaca aggtcaagct gcgcggcacg
      901 aactttccct ccgatggccc ggtgatgcag aagaagacca tgggctggga ggcttccagc
      961 gagcgcatgt atcccgagga cggcgctctg aagggcgaga tcaagcagcg cctgaagctg
     1021 aaggatggag gccactacga cgccgaggtg aagaccacct acaaggccaa gaagcccgtg
     1081 caattgcccg gcgcctacaa cgtgaacatc aagctggaca tcaccagcca caacgaggac
     1141 tacaccatcg tggagcagta cgagcgtgcc gaaggacgtc atagcacggg cggcatggat
     1201 gagctgtaca agagtgcgag accactcatc gcccaatcac tagtgaattc gcggccgcct
     1261 gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga gtattctata
     1321 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1381 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1441 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1501 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1561 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2161 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2461 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2521 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2581 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2641 aatgataccg cgcgacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2701 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2761 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2821 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2881 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2941 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3001 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3061 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3121 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3181 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3241 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3301 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3361 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3421 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3481 atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     3541 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3601 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3661 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3721 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggc
//
