LOCUS       Exported                2241 bp ds-DNA     circular SYN 13-FEB-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(join(15..42,68..92))
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    93..182
                     /label=spy Term
     primer_bind     184..200
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        207..225
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     230..276
                     /label=ASP14AUG11
     primer_bind     235..276
                     /label=ASP14AUG15
     primer_bind     235..276
                     /label=ASP14AUG13
     primer_bind     236..276
                     /label=ASP14AUG03
     primer_bind     236..276
                     /label=ASP14AUG07
     primer_bind     236..276
                     /label=SENSE_PRM
     primer_bind     243..276
                     /label=SENSE_PRM
     primer_bind     243..276
                     /label=ASP14AUG09
     primer_bind     243..276
                     /label=ASP14AUG01
     primer_bind     243..276
                     /label=SENSE_PRM
     primer_bind     249..276
                     /label=ASP14AUG05
     promoter        260..307
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_feature    327..332
                     /label=Shine-Dalgarno
     CDS             341..628
                     /codon_start=1
                     /label=LacZ Alfa1
                     /translation="MTMITPSYLGDTIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGE"
     misc_feature    389..444
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(690..751)
                     /label=ASP14AUG12
     primer_bind     complement(690..751)
                     /label=ASP14AUG08
     primer_bind     complement(690..751)
                     /label=ASP14AUG04
     primer_bind     complement(690..723)
                     /label=ASP14AUG10
     primer_bind     complement(690..723)
                     /label=ASP14AUG02
     primer_bind     complement(690..720)
                     /label=ASP14AUG16
     primer_bind     complement(690..720)
                     /label=ASP14AUG06
     primer_bind     complement(690..720)
                     /label=ASP14AUG14
     primer_bind     complement(693..751)
                     /label=ANTISENSE_PRM
     primer_bind     complement(693..723)
                     /label=ANTISENSE_PRM
     primer_bind     complement(693..723)
                     /label=ANTISENSE_PRM
     promoter        complement(754..772)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(790..806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(809..869)
                     /label=L3S2P21 Term
                     /note="Synthetic Terminator. Very Strong. See Chen, 2013 
                     (Chris Voigt)"
     misc_feature    879..923
                     /label= rrnB T1 Terminator
     primer_bind     complement(937..954)
                     /label=NM15OCT33
     primer_bind     947..972
                     /label=NM15MAY04
     rep_origin      1005..1361
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     complement(1342..1366)
                     /label=NM15MAY05
     primer_bind     1363..1389
                     /label=ASP15JUN28
     terminator      1367..1474
     CDS             complement(1478..2134)
                     /codon_start=1
                     /label=Chloramphenicol Resistance
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        2139..2237
                     /label=cat Promoter
     primer_bind     complement(2217..2241)
                     /label=ASP15JUN29
     primer_bind     join(2238..2241,1..26)
                     /label=NM15OCT31
ORIGIN
        1 attgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg
       61 cagtttatgg cgggcgtcct gcccgccacc ctttcagcca aaaaacttaa gaccgccggt
      121 cttgtccact accttgcagt aatgcggtgg acaggatcgg cggttttctt ttctcttctc
      181 aatgtaaaac gacggccagt gaattgtaat acgactcact atagggttcc tgcaggtctc
      241 agtcaggaga gagacgcctg ttgacaatta atcatcggca tagtatatcg gcatagtata
      301 atacgacaag gtgaggaact aaacccagga ggcagagatc atgaccatga ttacgccaag
      361 ctatctgggc gatactatcg aatactctag cttgcatgcg tgcaggtcga ctctagaaga
      421 tccccgggta ccgagctcta attcgccgta cagcgagtct tactacaatt ctctggcggt
      481 ggtcttgcaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      541 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      601 acagttgcgc agcctgaatg gcgaatgacg cctgatgcgg tattttctcc ttacgttact
      661 gtgcggtatt tcacacgtgc acctcagtac aatctgctct gatgccgcat agcgtctctc
      721 gcttgagacc tgcagtgact tgtattccgc ggttctatag tgtcacctaa atagcttggc
      781 gtaatttagg tcatagctgt ttcctgtcgg accaaaacga aaaaaggccc ccctttcggg
      841 aggcctcttt tctggaattt ggtaccgagc aggcatcaaa taaaacgaaa ggctcagtcg
      901 aaagactggg cctttcgttt tatctgttgt ttgtcggttg aacgctctct gacgtaccgc
      961 ggccgcgtag aggatctgtt gatcagcagt tcaacctgtt gatagtacgt actaagctct
     1021 catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt taacgaacta
     1081 aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct catgtttcac
     1141 gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt aaatagcctc
     1201 taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag cttttaaggt
     1261 ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt agagcctctc
     1321 aaagcaattt tgagtgacac aggaacactt aacggctgac atgtgatcat gtttgacagc
     1381 ttatcatcga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaaccag
     1441 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcgtcgc
     1501 aatactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1561 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtatagtat ttgcccatcg
     1621 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1681 tcacccaggg attggctgac acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1741 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1801 cgtcgtggta ttcactccag aggcttgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1861 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1921 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1981 tatttttctt tacggtcttt aaaaaggccg taatatccag ttgaacggtc tggttatagg
     2041 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2101 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2161 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2221 ctcttacgtg ccgatcataa c
//
