LOCUS       Exported                4381 bp ds-DNA     circular SYN 11-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     12..31
                     /label=NM17APR08
     primer_bind     21..36
                     /label=NM17APR01
     promoter        24..235
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(221..235)
                     /label=NM17APR03
     primer_bind     complement(225..239)
                     /label=NM17APR02
     primer_bind     235..257
                     /label=ASP14OCT56
     CDS             237..944
                     /codon_start=1
                     /product="Codon optimized for DM"
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     237..257
                     /label=ASP15FEB25
     primer_bind     complement(925..949)
                     /label=ASP15FEB26
     primer_bind     complement(925..944)
                     /label=ASP14OCT57
     primer_bind     944..1005
                     /label=NM18JUN17
     misc_feature    complement(951..984)
                     /label=loxP
     primer_bind     984..1011
                     /label=NM17JUN11
     promoter        985..1044
                     /label=SPL20
     primer_bind     985..1011
                     /label=NM17JUN09
     primer_bind     complement(1021..1048)
                     /label=NM17JUN10
     CDS             1046..1906
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSSIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1046..1067
                     /label=ASP14NOV44
     primer_bind     1907..1922
                     /label=NM15OCT08
     terminator      1911..2032
                     /label=T7
     terminator      1960..2007
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2007..2032)
                     /label=NM15OCT09
     primer_bind     complement(2014..2072)
                     /label=NM18JUN16
     primer_bind     complement(2014..2068)
                     /label=NM18JUN18
     misc_feature    complement(2033..2066)
                     /label=loxP
     CDS             2072..2106
                     /codon_start=1
                     /label=GGS4 Linker
                     /translation="GGSGGSGGSGG"
     primer_bind     2125..2150
                     /label=ASP14NOV50
     rep_origin      complement(2212..2894)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(2907..2932)
                     /label=ASP14NOV51
     primer_bind     2929..2953
                     /label=ASP14JUL17
     promoter        2933..3031
                     /label=cat Promoter
     primer_bind     complement(3016..3035)
                     /label=ASP14OCT52
     primer_bind     3032..3054
                     /label=ASP14OCT53
     CDS             3036..3848
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3830..3853)
                     /label=ASP14OCT54
     primer_bind     3849..3875
                     /label=ASP14OCT55
     terminator      3853..3960
     primer_bind     complement(3941..3964)
                     /label=ASP14JUL20
     primer_bind     3961..3984
                     /label=ASP14NOV52
     rep_origin      4008..4314
                     /label=F1 ori
     primer_bind     complement(join(4363..4381,1..6))
                     /label=ASP14NOV53
ORIGIN
        1 taacgaattc gtctcaggag tatgggcaga gcgcacatcg cccacagtcc ccgagaagtt
       61 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
      121 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
      181 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagaatgg
      241 tgtccaaggg agaagaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtcc
      301 acatggaggg cagcgtgaat ggccacgagt tcgagatcga gggcgaaggc gagggacgtc
      361 cctacgaagg cacccagacc gccaaactga aggtgaccaa gggaggcccg ttgccctttg
      421 cctgggacat cttgagcccc cagttcatgt acggctccaa ggcctacgtg aagcacccgg
      481 ccgacattcc cgactacctg aagctgagct tccccgaagg cttcaagtgg gagcgcgtga
      541 tgaacttcga ggacggcgga gtggtgaccg tgacccagga ctccagcttg caggacggcg
      601 agttcatcta caaggtcaag ctgcgcggca cgaactttcc ctccgatggc ccggtgatgc
      661 agaagaagac catgggctgg gaggcttcca gcgagcgcat gtatcccgag gacggcgctc
      721 tgaagggcga gatcaagcag cgcctgaagc tgaaggatgg aggccactac gacgccgagg
      781 tgaagaccac ctacaaggcc aagaagcccg tgcaattgcc cggcgcctac aacgtgaaca
      841 tcaagctgga catcaccagc cacaacgagg actacaccat cgtggagcag tacgagcgtg
      901 ccgaaggacg tcatagcacg ggcggcatgg atgagctgta caagagtgcc ataacttcgt
      961 ataatgtatg ctatacgaag ttataatact tgacatatca ctgtgattca catataatat
     1021 gcgatacgta aggaggttag acataatgag tattcaacat ttccgtgtgg cgctgatccc
     1081 cttttttgct gcattctgtt tgccagtatt cgcccatcca gaaacgctgg tcaaagttaa
     1141 ggatgcagag gatcaacttg gtgctcgtgt cggttacatt gagttggacc ttaattccgg
     1201 aaaaatctta gagagcttcc gccctgaaga acgtttcccc atgatgagta ccttcaaagt
     1261 gttgttgtgc ggcgcggttt taagctccat cgatgcaggt caagagcaac tgggccgtcg
     1321 tatccactac tcccagaatg atcttgtgga atactccccc gtaacagaga agcatttgac
     1381 tgatggcatg acagttcgtg aactgtgtag tgccgctatt acgatgtctg ataacaccgc
     1441 cgccaacctt ctgcttacga ccattggcgg tccgaaggag ctgacggcat tccttcacaa
     1501 catgggcgat cacgtcactc gtttagaccg ctgggagccg gaattaaacg aagctatccc
     1561 caatgacgaa cgtgacacga cgatgcccgt cgctatggcc acaacgctgc gtaaattgct
     1621 gactggagag cttttaaccc tggcatctcg ccagcaactt atcgattgga tggaggcaga
     1681 taaagtggcc gggccgctgc tgcgctctgc cctgccagcc ggatggttta ttgcggataa
     1741 gtctggggcg ggagaacgcg gttctcgtgg cattattgca gcacttggtc cggatgggaa
     1801 accctcacgt attgtggtaa tctatacgac aggctcccag gcgacgatgg acgagcgtaa
     1861 ccgtcagatc gcagaaatcg gggcttctct tattaagcat tggtaagctt caaagcccga
     1921 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     1981 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atataacttc
     2041 gtataatgta tgctatacga agttatccct cggaggttcc ggtggaagcg gaggtagcgg
     2101 cggcagcgct gtcatgagac gaattctgac acggttatcc acagaatcag gggataacgc
     2161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2641 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     2701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgt gatgatcggc
     2941 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
     3001 agttatcgag attttcagga gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt
     3061 cttgctcaag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     3121 ctcgcgataa tgtcgggcaa tcaggtgcga caatctaccg attgtatggg aagcccgatg
     3181 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     3241 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     3301 gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg
     3361 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     3421 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgac
     3481 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     3541 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     3601 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     3661 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     3721 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     3781 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3841 agtttttctg atttttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
     3901 tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg tcaaacatga
     3961 tgttcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     4021 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4081 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4141 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4201 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4261 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4321 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4381 t
//
