LOCUS       Exported                6311 bp ds-DNA     circular SYN 15-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     17..40
                     /label=ASP14OCT13
     primer_bind     complement(238..262)
                     /label=ASP14OCT14
     primer_bind     258..278
                     /label=ASP14OCT15
     exon            347..576
                     /label=Exon1
     intron          577..977
     primer_bind     628..650
                     /label=NSA14OCT01
     exon            978..1251
                     /label=Exon2
     iDNA            1252..1325
                     /label=Intron
     primer_bind     1288..1311
                     /label=NSA140CT02
     exon            1326..1980
                     /label=Exon3
     intron          1981..2041
     exon            2042..2357
                     /label=Exon4
     primer_bind     2056..2078
                     /label=NSA15Jan01
                     /note="For resequencing poor region"
     primer_bind     2203..2224
                     /label=NSA140CT03
     intron          2358..2560
     exon            2561..2692
                     /label=Exon5
     intron          2693..2762
     exon            2763..3508
                     /label=Exon6
     primer_bind     3101..3122
                     /label=NSA140CT04
     primer_bind     complement(3241..3260)
                     /label=ASP14OCT16
     primer_bind     complement(3817..3837)
                     /label=ASP14OCT18
     primer_bind     3834..3853
                     /label=ASP14OCT19
     primer_bind     complement(4017..4051)
                     /label=ASP14OCT20
     primer_bind     4055..4080
                     /label=ASP14NOV50
     rep_origin      complement(4142..4824)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(4837..4862)
                     /label=ASP14NOV51
     primer_bind     4859..4883
                     /label=ASP14JUL17
     promoter        4863..4961
                     /label=cat Promoter
     primer_bind     complement(4946..4965)
                     /label=ASP14OCT52
     primer_bind     4962..4984
                     /label=ASP14OCT53
     CDS             4966..5778
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5760..5783)
                     /label=ASP14OCT54
     primer_bind     5779..5805
                     /label=ASP14OCT55
     terminator      5783..5890
     primer_bind     complement(5871..5894)
                     /label=ASP14JUL20
     primer_bind     5891..5914
                     /label=ASP14NOV52
     rep_origin      5938..6244
                     /label=F1 ori
     primer_bind     complement(join(6293..6311,1..6))
                     /label=ASP14NOV53
ORIGIN
        1 taacgaattc gtctcaggag gaattctagt atgtatgtaa gttaataaaa cccatttttg
       61 cggaaagtag ataaaaaaaa catttttttt ttttactgca ctggatatca ttgaacttat
      121 ctgatcagtt ttaaatttac ttcgatccaa gggtatttga tgtaccaggt tctttcgatt
      181 acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc
      241 acagaaatat cgccgtcact ttcgccgctg cgtccgctat ctctttcgcc accgtttgta
      301 gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg tcagcggttt cgtgacgaag
      361 ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt
      421 cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag
      481 gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga
      541 ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc
      601 ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga
      661 actgcgaccc acaaaaatcc caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga
      721 ttattctggt agctgttctc gctatataag acaatttttg agatcatatc atgatcaaga
      781 catctaaagg cattcatttt cgactatatt cttttttaca aaaaatataa caaccagata
      841 ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat
      901 acttcggggt actttttgtt cggggttaga tgagcataac gcttgtagtt gatatttgag
      961 atcccctatc attgcagggt gacagcggag cggcttcgca gagctgcatt aaccagggct
     1021 tcgggcaggc caaaaactac ggcacgctcc ggccacccag tccgccggag gactccggtt
     1081 cagggagcgg ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg
     1141 gggcggtcaa tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat
     1201 tctgcaacga gcgacacata ccggcgccca ggaaacattt gctcaagaac ggtgagtttc
     1261 tattcgcagt cggctgatct gtgtgaaatc ttaataaagg gtccaattac caatttgaaa
     1321 ctcagtttgc ggcgtggcct atccgggcga acttttggcc gtgatgggca gttccggtgc
     1381 cggaaagacg accctgctga atgcccttgc ctttcgatcg ccgcagggca tccaagtatc
     1441 gccatccggg atgcgactgc tcaatggcca acctgtggac gccaaggaga tgcaggccag
     1501 gtgcgcctat gtccagcagg atgacctctt tatcggctcc ctaacggcca gggaacacct
     1561 gattttccaa gccatggtgc ggatgccacg acatctgacc tatcggcagc gagtggcccg
     1621 cgtggatcag gtgatccagg agctttcgct cagcaaatgt cagcacacga tcatcggtgt
     1681 gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat tcgcctccga
     1741 ggctctaacc gatccgccgc ttctgatctg cgatgagccc acctccggac tggactcctt
     1801 taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca agaccgtcat
     1861 cctgaccatt catcagccgt cttccgagct gtttgagctc tttgacaaga tccttctgat
     1921 ggccgagggc agggtagctt tcttgggcac tcccagcgaa gccgtcgact tcttttccta
     1981 gtgagttcga tgtgtttatt aagggtatct agtattacat aacatctcaa ctcctatcca
     2041 gcgtgggtgc ccagtgtcct accaactaca atccggcgga cttttacgta caggtgttgg
     2101 ccgttgtgcc cggacgggag atcgagtccc gtgatcggat cgccaagata tgcgacaatt
     2161 ttgccattag caaagtagcc cgggatatgg agcagttgtt ggccaccaaa aatctggaga
     2221 agccactgga gcagccggag aatgggtaca cctacaaggc cacctggttc atgcagttcc
     2281 gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga accactcctc gtaaaagtgc
     2341 gacttattca gacaacggtg agtggttcca gtggaaacaa atgatataac gcttacaatt
     2401 cttggaaaca aattcgctag attttagata gaattgcctg attccacacc cttcttagtt
     2461 tttttcaatg agatgtatag tttatagttt tgcagaagat aaataaattt catttaactc
     2521 gcgaatatta atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg
     2581 cctcatcttt ttgggccaac aactcacgca agtgggtgtg atgaatatca acggagccat
     2641 cttcctcttc ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtca
     2701 tgtttagaat acatttgcat ttcaataatt tactaacttt ctaatgaatc gattcgattt
     2761 aggtgttcac ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct
     2821 gtgacacata ctttctgggc aaaacgattg ccgaattgcc gctttttctc acagtgccac
     2881 tggtcttcac ggcgattgcc tatccgatga tcggactgcg ggccggagtg ctgcacttct
     2941 tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc ggatatctaa
     3001 tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt gggtccgccg gttatcatac
     3061 cattcctgct ctttggcggc ttcttcttga actcgggctc ggtgccagta tacctcaaat
     3121 ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt aaccaatggg
     3181 cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc cccagttcgg
     3241 gcaaggtcat cctggagaca cttaacttct ccgccgccga tctgccgctg gactacgtgg
     3301 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     3361 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt ttttttacca
     3421 ttattaccat cgtgtttact gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt
     3481 gccaataaaa acaagatatg acctatagaa tacaagtatt tccccttcga acatccccac
     3541 aagtagactt tggatttgtc ttctaaccaa aagacttaca cacctgcata ccttacatca
     3601 aaaactcgtt tatcgctaca taaaacaccg ggatatattt tttatataca tacttttcaa
     3661 atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg tttcgtagtt gctctttcgc
     3721 tgtctcccac ccgctctccg caacacattc accttttgtt cgacgacctt ggagcgactg
     3781 tcgttagttc cgcgcgattc ggttcgctca aatggttccg agtggttcat ttcggctcaa
     3841 tagaaattag taataaatat ttgtatgtac aatttatttg ctccaatata tttgtatata
     3901 tttccctcac agctatattt attctaattt aatattatga ctttttaagg taattttttg
     3961 tgacctgttc ggagtgatta gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt
     4021 ccttgtgtag atgcatcgct gtcatgagac gaattctgac acggttatcc acagaatcag
     4081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4561 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgt
     4861 gatgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     4921 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag ccatattcaa
     4981 cgggaaacgt cttgctcaag gccgcgatta aattccaaca tggatgctga tttatatggg
     5041 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctaccg attgtatggg
     5101 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     5161 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     5221 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca
     5281 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     5341 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     5401 gtatttcgac tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     5461 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     5521 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     5581 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     5641 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     5701 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     5761 atgctcgatg agtttttctg atttttttta aggcagttat tggtgccctt aaacgcctgg
     5821 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg
     5881 tcaaacatga tgttcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     5941 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6001 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6061 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6121 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6181 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6241 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6301 gcctattggt t
//
