LOCUS       Exported                3683 bp ds-DNA     circular SYN 31-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     12..30
                     /label=NM16NOV22
     primer_bind     17..42
                     /label=ASP14NOV39
     promoter        21..475
                     /label=Hsp70B
     primer_bind     21..42
                     /label=NM17FEB01
     primer_bind     21..41
                     /label=NM18AUG01
     primer_bind     complement(109..134)
                     /label=ASP14NOV40
     primer_bind     130..151
                     /label=ASP14NOV41
     primer_bind     complement(450..482)
                     /label=NM16NOV21
     primer_bind     complement(450..475)
                     /label=ASP14NOV42
     primer_bind     476..500
                     /label=ASP14SEP09
     promoter        480..547
                     /label=CP6 Promoter
     primer_bind     complement(543..564)
                     /label=NM15OCT02
     mRNA            548..553
                     /label=SD
     misc_feature    558..560
                     /label=Kozak
     primer_bind     561..582
                     /label=Mock NM15SEP10
     CDS             562..1356
                     /codon_start=1
                     /product="Aminoglycoside Phosphtransferase gene. Confers 
                     Resistance to Neomycin (G418) in eukaroyotic cells and 
                     Kanamycin in prokaryotes.  "
                     
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1337..1360)
                     /label=Mock NM15SEP11
     misc_feature    1361..1408
                     /label=HSV-TK
     primer_bind     complement(1386..1408)
                     /label=NM18AUG02
     primer_bind     1427..1452
                     /label=ASP14NOV50
     rep_origin      complement(1514..2196)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(2209..2234)
                     /label=ASP14NOV51
     primer_bind     2231..2255
                     /label=ASP14JUL17
     promoter        2235..2333
                     /label=cat Promoter
     primer_bind     complement(2318..2337)
                     /label=ASP14OCT52
     primer_bind     2334..2356
                     /label=ASP14OCT53
     CDS             2338..3150
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3132..3155)
                     /label=ASP14OCT54
     primer_bind     3151..3177
                     /label=ASP14OCT55
     terminator      3155..3262
     primer_bind     complement(3243..3266)
                     /label=ASP14JUL20
     primer_bind     3263..3286
                     /label=ASP14NOV52
     rep_origin      3310..3616
                     /label=F1 ori
     primer_bind     complement(join(3665..3683,1..6))
                     /label=ASP14NOV53
ORIGIN
        1 taacgaattc gtctcaggag ctagaatccc aaaacaaact ggttattgtg gtaggtcatt
       61 tgtttggcag aaagaaaact cgagaaattt ctctggccgt tattcgttat tctctctttt
      121 ctttttgggt cgctccctct ctgcactaat gctctctcac tctgtcacac agtaaacggc
      181 atactgctct cgttggttcg agagagcgcg cctcgaatgt tcgcgaaaag agcgccggag
      241 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
      301 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
      361 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
      421 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctcaatactc
      481 gaatccatgt gggagtttat tcttgacaca gatatttatg atataataac tgagtaagct
      541 taacataagg aggaaaacaa aatgattgag caggatggac tgcacgctgg ctcgccagct
      601 gcctgggtgg agcgtctgtt cggctacgat tgggcccagc agaccatcgg ctgctccgat
      661 gctgccgtgt tccgtctgtc ggcccaggga cgccccgtgc tgttcgtcaa gaccgacctg
      721 agtggagccc tgaacgagct ccaggatgag gcagctcgtc tgagctggct ggccacaaca
      781 ggagtgccgt gtgctgccgt gctggatgtg gtgaccgaag ctggacgcga ttggctgctg
      841 ctgggcgagg tgccaggcca ggacctgctg tccagccatc tggccccagc cgagaaggtg
      901 tccattatgg ccgatgctat gcgtcgcctg cacaccctgg acccagccac ctgccccttt
      961 gaccaccagg ccaagcaccg tattgagcgt gcccgaaccc gtatggaggc tggactggtg
     1021 gaccaggatg acctggatga ggagcaccag ggcctggctc cagccgagct gtttgcccgt
     1081 ctgaaggccc gtatgcccga tggcgaggac ctggtggtga cccacggcga cgcctgcctg
     1141 cccaacatta tggtggagaa cggacgcttc agtggcttca ttgattgtgg acgcctgggc
     1201 gtggccgacc gttaccagga tatcgccctg gccacccgag atatcgccga ggagctggga
     1261 ggcgagtggg ccgaccgatt cctggtgctc tacggcattg cagccccaga tagccagcgt
     1321 attgccttct accgtctgct ggacgagttc ttctaagctt ggcaataaaa agacagaata
     1381 aaacgcacgg gtgttgggtc gtttgttccg ctgtcatgag acgaattctg acacggttat
     1441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     1981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2221 agatcctttt gtgatgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
     2281 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg
     2341 agccatattc aacgggaaac gtcttgctca aggccgcgat taaattccaa catggatgct
     2401 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctac
     2461 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2521 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2581 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2641 ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2701 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2761 aacagcgatc gcgtatttcg actcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2821 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2881 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2941 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3001 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3061 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3121 cagtttcatt tgatgctcga tgagtttttc tgattttttt taaggcagtt attggtgccc
     3181 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     3241 gatgataagc tgtcaaacat gatgttcaca tttccccgaa aagtgccacc tgacgcgccc
     3301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3421 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3541 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3601 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3661 ttgccgattt cggcctattg gtt
//
