LOCUS       Exported                4059 bp ds-DNA     circular SYN 24-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     11..47
                     /label=ASP14NOV39
     primer_bind     11..35
                     /label=NM16NOV22
     promoter        26..480
                     /label=Hsp70B
     primer_bind     complement(114..139)
                     /label=ASP14NOV40
     primer_bind     135..156
                     /label=ASP14NOV41
     primer_bind     complement(455..487)
                     /label=NM16NOV21
     primer_bind     complement(455..480)
                     /label=ASP14NOV42
     primer_bind     481..505
                     /label=ASP14SEP09
     promoter        485..552
                     /label=CP6 Promoter
     primer_bind     complement(548..569)
                     /label=NM15OCT02
     mRNA            553..558
                     /label=SD
     misc_feature    563..565
                     /label=Kozak
     CDS             567..1697
                     /codon_start=1
                     /label=Thymidine Kinase
                     /translation="MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQKATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTIFLDR
                     HPIAFMLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(1158..1169)
                     /label=NM15DEC06
     primer_bind     complement(1260..1272)
                     /label=NM15DEC06
     polyA_site      1702..1749
                     /label=HSV-TK PolyA
                     /note="No BCD"
     primer_bind     1766..1789
                     /label=ASP14NOV50
     rep_origin      complement(1851..2533)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(2546..2571)
                     /label=ASP14NOV51
     primer_bind     2568..2592
                     /label=ASP14JUL17
     promoter        2572..2670
                     /label=cat Promoter
     primer_bind     complement(2655..2674)
                     /label=ASP14OCT52
     primer_bind     2671..2693
                     /label=ASP14OCT53
     CDS             2675..3487
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3469..3492)
                     /label=ASP14OCT54
     primer_bind     3488..3514
                     /label=ASP14OCT55
     terminator      3492..3599
     primer_bind     complement(3580..3603)
                     /label=ASP14JUL20
     primer_bind     3600..3623
                     /label=ASP15JAN01
     rep_origin      3604..4059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(join(4028..4059,1..4))
                     /label=ASP15JUL28
ORIGIN
        1 taacaagctt cgtctcagtc aggagctaga atcccaaaac aaactggtta ttgtggtagg
       61 tcatttgttt ggcagaaaga aaactcgaga aatttctctg gccgttattc gttattctct
      121 cttttctttt tgggtcgctc cctctctgca ctaatgctct ctcactctgt cacacagtaa
      181 acggcatact gctctcgttg gttcgagaga gcgcgcctcg aatgttcgcg aaaagagcgc
      241 cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt
      301 gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa
      361 tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc
      421 aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctcaa
      481 tactcgaatc catgtgggag tttattcttg acacagatat ttatgatata ataactgagt
      541 aagcttaaca taaggaggaa aacaaaatgg ccagctaccc ctgccatcag cacgccagcg
      601 ccttcgatca ggctgcccgc agccgcggac acaacaaccg tcgcaccgcc ctgcgcccac
      661 gccgccagca gaaggccacc gaagtccgct tggagcagaa gatgcccaca ctgctgcgcg
      721 tgtacatcga tggcccccat ggcatgggca agaccaccac gacccaactg ctggtcgccc
      781 tgggcagccg cgacgacatc gtctacgtgc ccgagcccat gacctactgg caagtcctgg
      841 gcgcctccga gacaattgcc aacatctaca ccacccagca ccgcttggat cagggcgaga
      901 tcagtgccgg cgacgccgcc gtggtgatga ccagcgccca gatcaccatg ggaatgccct
      961 acgccgtgac cgatgccgtc ctggcccccc acattggagg cgaggccggc agctcgcatg
     1021 ccccaccccc agccctgaca atcttcctgg atcgccaccc gatcgccttc atgctgtgct
     1081 acccagccgc ccgttatctg atgggcagca tgacccccca ggccgtgctg gccttcgtgg
     1141 ccctgatccc cccgaccctg cccggcacca acatcgtgct gggagccctg cccgaggatc
     1201 gccacatcga ccgcctggcc aagcgccagc gtccaggaga gcgcctggat ctggccatgc
     1261 tggccgccat ccgccgcgtg tacggcctgc tggccaacac cgtgcgctat cttcaaggag
     1321 gcggctcctg gcgcgaggat tggggccaac tgtccggaac ggccgtgcca ccccagggag
     1381 ccgagcccca gagcaatgcc ggcccacgcc cccacatcgg cgataccctg tttacgctgt
     1441 tccgtgcccc cgagctgctg gccccgaacg gcgatttgta caatgtgttc gcctgggccc
     1501 tggatgtgct ggcgaagcgt ctgcgcccca tgcacgtgtt catcctggat tacgatcaaa
     1561 gcccagccgg atgccgcgac gccctcttgc aattgacaag cggcatggtg cagacccatg
     1621 tgaccacccc cggcagcatc cccacaatct gcgatctggc ccgcacattc gcccgcgaga
     1681 tgggcgaggc taattaagct tggcaataaa aagacagaat aaaacgcacg ggtgttgggt
     1741 cgtttgttcc gcttgagacg aagcttgaca cggttatcca cagaatcagg ggataacgca
     1801 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1861 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1921 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1981 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2041 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2101 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2161 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2221 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2281 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2341 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2401 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2461 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2521 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgtg atgatcggca
     2581 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     2641 gttatcgaga ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc
     2701 ttgctcaagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2761 tcgcgataat gtcgggcaat caggtgcgac aatctaccga ttgtatggga agcccgatgc
     2821 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2881 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2941 tactcctgat gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt
     3001 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     3061 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgact
     3121 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3181 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     3241 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3301 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3361 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3421 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3481 gtttttctga ttttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct
     3541 gaataagtga taataagcgg atgaatggca gaaattcgat gataagctgt caaacatgat
     3601 gttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3661 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3721 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3781 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3841 ggccagcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3901 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3961 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4021 ttaacgcgaa ttttaacaaa atattaacgc ttacaattt
//
