LOCUS       Exported                4050 bp ds-DNA     circular SYN 22-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     11..47
                     /label=ASP14NOV39
     primer_bind     11..35
                     /label=NM16NOV22
     promoter        26..480
                     /label=Hsp70B
     primer_bind     complement(114..139)
                     /label=ASP14NOV40
     primer_bind     135..156
                     /label=ASP14NOV41
     primer_bind     complement(455..487)
                     /label=NM16NOV21
     primer_bind     complement(455..480)
                     /label=ASP14NOV42
     primer_bind     481..505
                     /label=ASP14SEP09
     promoter        485..552
                     /label=CP6 Promoter
     primer_bind     complement(548..569)
                     /label=NM15OCT02
     mRNA            553..558
                     /label=SD
     misc_feature    563..565
                     /label=Kozak
     CDS             567..1688
                     /codon_start=1
                     /label=FCU1
                     /translation="MVTGGMASKWDQKGMDIAYEEAALGYKEGGVPIGGCLINNKDGSV
                     LGRGHNMRFQKGSATLHGEISTLENCGRLEGKVYKDTTLYTTLSPCDMCTGAIIMYGIP
                     RCVVGENVNFKSKGEKYLQTRGHEVVVVDDERCKKIMKQFIDERPQDWFEDIGEASEPF
                     KNVYLLPQTNQLLGLYTIIRNKNTTRPDFIFYCDRIIRLLVEEGLNHLPVQKQIVETDT
                     NENFEGVSFMGKICGVSIVRAGESMEQGLRDCCRSVRIGKILIQRDEETALPKLFYEKL
                     PEDISERYVFLLDPMLATGGSAIMATEVLIKRGVKPERIYFLNLICSKEGIEKYHAAFP
                     EVRIVTGALDRGLDENKYLVPGLGDFGDRYYCV"
     polyA_site      1693..1740
                     /label=HSV-TK PolyA
                     /note="No BCD"
     primer_bind     1757..1780
                     /label=ASP14NOV50
     rep_origin      complement(1842..2524)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(2537..2562)
                     /label=ASP14NOV51
     primer_bind     2559..2583
                     /label=ASP14JUL17
     promoter        2563..2661
                     /label=cat Promoter
     primer_bind     complement(2646..2665)
                     /label=ASP14OCT52
     primer_bind     2662..2684
                     /label=ASP14OCT53
     CDS             2666..3478
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3460..3483)
                     /label=ASP14OCT54
     primer_bind     3479..3505
                     /label=ASP14OCT55
     terminator      3483..3590
     primer_bind     complement(3571..3594)
                     /label=ASP14JUL20
     primer_bind     3591..3614
                     /label=ASP15JAN01
     rep_origin      3595..4050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(join(4019..4050,1..4))
                     /label=ASP15JUL28
ORIGIN
        1 taacaagctt cgtctcagtc aggagctaga atcccaaaac aaactggtta ttgtggtagg
       61 tcatttgttt ggcagaaaga aaactcgaga aatttctctg gccgttattc gttattctct
      121 cttttctttt tgggtcgctc cctctctgca ctaatgctct ctcactctgt cacacagtaa
      181 acggcatact gctctcgttg gttcgagaga gcgcgcctcg aatgttcgcg aaaagagcgc
      241 cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt
      301 gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa
      361 tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc
      421 aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctcaa
      481 tactcgaatc catgtgggag tttattcttg acacagatat ttatgatata ataactgagt
      541 aagcttaaca taaggaggaa aacaaaatgg tgaccggcgg catggccagc aagtgggatc
      601 aaaagggcat ggatattgcc tatgaggagg ccgccttggg ctacaaggag ggcggcgtcc
      661 ccattggcgg ctgcttgatc aacaacaagg atggcagcgt gctgggacgc ggccacaaca
      721 tgcgcttcca gaagggcagc gccaccttgc acggcgagat cagcaccctg gagaactgcg
      781 gacgcttgga gggcaaggtg tacaaggata ccaccctgta caccaccctg agcccctgcg
      841 atatgtgcac cggcgccatc atcatgtacg gcatcccccg ctgcgtggtg ggcgagaacg
      901 tgaacttcaa gagcaagggc gagaagtatc tgcaaacccg cggacacgag gtggtggtgg
      961 tggatgatga gcgatgcaaa aagatcatga agcagttcat cgatgagcgc ccccaggatt
     1021 ggttcgagga tattggcgag gccagcgagc ccttcaagaa cgtgtatctg ctgccccaga
     1081 ccaaccaact gctgggcctg tacaccatca tccgcaacaa gaacaccacc cgcccagatt
     1141 tcatcttcta ttgtgaccgc atcatccgcc tgctggtcga ggagggcctg aaccacctgc
     1201 ccgtgcagaa gcagatcgtc gaaaccgata ccaacgagaa cttcgagggc gttagcttca
     1261 tgggcaagat ctgcggcgtg agcatcgtgc gtgccggcga gagcatggag cagggcctgc
     1321 gcgattgctg ccgcagcgtg cgcatcggca agatcctgat ccagcgcgac gaggaaaccg
     1381 ccctgcccaa gctgttttac gagaagctgc ccgaggatat ttccgagcgc tacgtgttcc
     1441 tgctcgatcc catgctggcc accggcggca gcgccatcat ggccaccgag gtgctgatca
     1501 agcgcggcgt gaagcccgag cgcatctact ttctgaacct gatttgcagc aaggagggca
     1561 ttgagaagta ccacgccgcc ttcccggagg tccgcatcgt gaccggagcc ttggatcgcg
     1621 gattggatga gaacaaatat ctggtgcccg gcctgggcga tttcggcgat cgctactact
     1681 gcgtgtaagc ttggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     1741 cgcttgagac gaagcttgac acggttatcc acagaatcag gggataacgc aggaaagaac
     1801 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1861 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2041 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2161 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2221 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2281 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2341 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2401 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2461 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2521 atgagattat caaaaaggat cttcacctag atccttttgt gatgatcggc acgtaagagg
     2581 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
     2641 attttcagga gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcaag
     2701 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2761 tgtcgggcaa tcaggtgcga caatctaccg attgtatggg aagcccgatg cgccagagtt
     2821 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2881 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2941 tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga
     3001 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     3061 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgac tcgctcaggc
     3121 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     3181 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     3241 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     3301 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     3361 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3421 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttctg
     3481 atttttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg
     3541 ataataagcg gatgaatggc agaaattcga tgataagctg tcaaacatga tgttacgcgc
     3601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccagcgc
     3841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4021 attttaacaa aatattaacg cttacaattt
//
