LOCUS       Exported               10029 bp ds-DNA     circular SYN 27-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    14..43
                     /label=STUFFER
     misc_feature    52..76
                     /label=SIE
     primer_bind     90..115
                     /label=ASP16SEP15
     primer_bind     90..115
                     /label=ASP16SEP17
     protein_bind    94..205
                     /label=attB_TT
                     /bound_moiety="phage phi-C31 integrase"
     primer_bind     94..130
                     /label=ASP14JUL13
     primer_bind     94..113
                     /label=ASP14NOV45
     misc_feature    139..172
                     /label=attB_TT
     primer_bind     complement(181..209)
                     /label=ASP16SEP16
     primer_bind     complement(181..209)
                     /label=ASP16SEP18
     primer_bind     complement(186..206)
                     /label=ASP14NOV46
     primer_bind     211..239
                     /label=ASP14NOV39
     primer_bind     211..227
                     /label=NM16NOV22
     promoter        218..672
                     /label=Hsp70B
     primer_bind     218..238
                     /label=NM18AUG01
     primer_bind     complement(306..331)
                     /label=ASP14NOV40
     primer_bind     327..348
                     /label=ASP14NOV41
     primer_bind     complement(647..679)
                     /label=NM16NOV21
     primer_bind     complement(647..672)
                     /label=ASP14NOV42
     primer_bind     673..697
                     /label=ASP14SEP09
     promoter        677..744
                     /label=CP6 Promoter
     primer_bind     677..697
                     /label=NM15DEC18
     primer_bind     677..692
                     /label=NM19JUN07
     primer_bind     679..699
                     /label=NM20MAR03
     primer_bind     complement(740..761)
                     /label=NM15OCT02
     mRNA            745..750
                     /label=SD
     primer_bind     complement(746..761)
                     /label=NM16NOV21
     misc_feature    755..757
                     /label=Kozak
     primer_bind     758..779
                     /label=Mock NM15SEP12
     CDS             759..1181
                     /codon_start=1
                     /product="Blasticidin S Resistance Gene (bsr). Produces 
                     Blasticidin S Deaminase and confers resistance to 
                     Blasticidin in prokaryotes and eukaryotes. BS is a 
                     nucleoside antibiotic that inhibits protein synthesis. "
                     /label=bsr
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     primer_bind     complement(1160..1185)
                     /label=Mock NM15SEP13
     misc_feature    1186..1233
                     /label=HSV-TK
     primer_bind     complement(1211..1233)
                     /label=NM18AUG02
     promoter        1246..1264
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1265..1287
                     /label=NM15SEP06
     primer_bind     1310..1346
                     /label=NM20MAR03
     promoter        1326..1391
                     /label=CP6 Promoter
     primer_bind     1326..1344
                     /label=ASP14SEP09
     primer_bind     1326..1344
                     /label=NM15DEC18
     primer_bind     1326..1339
                     /label=NM19JUN07
     primer_bind     complement(1387..1408)
                     /label=NM15OCT02
     mRNA            1392..1397
                     /label=SD
     primer_bind     complement(1393..1408)
                     /label=NM16NOV21
     primer_bind     1405..1425
                     /label=ASP14OCT05
     CDS             1406..2089
                     /label=Tinsel Purple
     primer_bind     complement(2067..2099)
                     /label=ASP14OCT06
     misc_feature    2093..2095
                     /product="3'"
                     /label=3'
     terminator      2100..2194
                     /label=T0
     primer_bind     complement(2169..2198)
                     /label=NM20MAR05
     primer_bind     complement(2173..2198)
                     /label=NM20MAR04
     primer_bind     2194..2218
                     /label=NM20MAR01
     misc_feature    2201..2257
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2240..2272)
                     /label=NM20MAR02
     promoter        complement(2319..2337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2342..2496
                     /label=DSCP
     primer_bind     2342..2357
                     /label=NM16NOV19
     primer_bind     2342..2357
                     /label=NM17JAN03
     TATA_signal     2370..2375
                     /label=TATA
     misc_feature    2399..2404
                     /label=Inr
                     /note="Inititator"
     misc_feature    2418..2427
                     /label=MTE
                     /note="Motif Ten Element"
     misc_feature    2428..2433
                     /label=DPE
                     /note="Downstream Promoter Element"
     primer_bind     complement(2485..2502)
                     /label=NM16NOV20
     primer_bind     2499..2521
                     /label=ASP14DEC02
     CDS             2500..2940
                     /codon_start=1
                     /label=Gal4BD 1-147 PMID 20697123
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     complement(2920..2944)
                     /label=ASP14DEC07
     CDS             2944..2987
                     /codon_start=1
                     /label=Linker GGGSx4
                     /translation="GGGSGGGSGGGSGG"
     primer_bind     2987..3011
                     /label=ASP14DEC08
     primer_bind     2991..3011
                     /label=ASP14DEC19
     CDS             2992..3333
                     /codon_start=1
                     /label=Gal4ADII PMID 20697123, 20697123 and 16582903
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    3244..3333
                     /label=Binding Domain
                     /bound_moiety="Gal80"
     primer_bind     complement(3314..3333)
                     /label=ASP14DEC21
     primer_bind     complement(3316..3340)
                     /label=ASP14DEC05
     primer_bind     3336..3365
                     /label=ASP14NOV59
     polyA_signal    3341..3536
                     /label=SV40 Late
     primer_bind     3341..3370
                     /label=NM19APR05
     polyA_signal    3347..3475
                     /label=SV40 Early (rev)
     primer_bind     3347..3370
                     /label=ASPNOV61
     primer_bind     complement(3451..3477)
                     /label=ASP14NOV62
     primer_bind     complement(3518..3540)
                     /label=ASP15APR19
     primer_bind     complement(3518..3537)
                     /label=ASP14NOV60
     primer_bind     complement(3526..3540)
                     /label=NM19APR06
     primer_bind     3542..3568
                     /label=ASP14OCT13
     primer_bind     complement(3766..3790)
                     /label=ASP14OCT14
     primer_bind     3786..3806
                     /label=ASP14OCT15
     exon            3875..4104
                     /label=Exon1
     intron          4105..4505
     primer_bind     4156..4178
                     /label=NSA14OCT01
     exon            4506..4779
                     /label=Exon2
     iDNA            4780..4853
                     /label=Intron
     primer_bind     4816..4839
                     /label=NSA140CT02
     exon            4854..5508
                     /label=Exon3
     intron          5509..5569
     exon            5570..5885
                     /label=Exon4
     primer_bind     5584..5606
                     /label=NSA15Jan01
                     /note="For resequencing poor region"
     primer_bind     5731..5752
                     /label=NSA140CT03
     intron          5886..6088
     exon            6089..6220
                     /label=Exon5
     intron          6221..6290
     exon            6291..7036
                     /label=Exon6
     primer_bind     6629..6650
                     /label=NSA140CT04
     primer_bind     complement(6769..6788)
                     /label=ASP14OCT16
     primer_bind     complement(7345..7365)
                     /label=ASP14OCT18
     primer_bind     7362..7381
                     /label=ASP14OCT19
     primer_bind     complement(7545..7568)
                     /label=ASP14OCT20
     primer_bind     7569..7592
                     /label=ASP14NOV50
     rep_origin      complement(7654..8336)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(8349..8374)
                     /label=ASP14NOV51
     primer_bind     8371..8393
                     /label=ASP14OCT46
     primer_bind     8371..8392
                     /label=ASP16AUG01
     promoter        8375..8508
                     /label=Spm promoter
     CDS             8509..9519
                     /codon_start=1
                     /gene="SpnR"
                     /label=SpmR
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      9520..9569
     primer_bind     complement(9547..9573)
                     /label=ASP16AUG02
     primer_bind     complement(9547..9570)
                     /label=ASP14OCT47
     primer_bind     9570..9593
                     /label=ASP15JAN01
     rep_origin      9574..10029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(join(9998..10029,1..5))
                     /label=ASP15JUL28
ORIGIN
        1 taacggatca gcgcagctgc ccgggtctag agtcgacgca tgcctcctga ctcgacgatt
       61 tacgggaaat gaagctctcc ggatcccagg gaggaattgg tcgacgatgt aggtcactgt
      121 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
      181 ctcacccatc tggtccatca tgatgcgctg tcaggagcta gaatcccaaa acaaactggt
      241 tattgtggta ggtcatttgt ttggcagaaa gaaaactcga gaaatttctc tggccgttat
      301 tcgttattct ctcttttctt tttgggtcgc tccctctctg cactaatgct ctctcactct
      361 gtcacacagt aaacggcata ctgctctcgt tggttcgaga gagcgcgcct cgaatgttcg
      421 cgaaaagagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
      481 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa
      541 caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
      601 accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa
      661 gagaactctc aatactcgaa tccatgtggg agtttattct tgacacagat atttatgata
      721 taataactga gtaagcttaa cataaggagg aaaacaaaat gaaaaccttc aacatcagcc
      781 agcaggacct ggaactggtg gaggtggcta ccgagaagat tacgatgctc tacgaggata
      841 acaagcacca cgtgggagct gccattcgca ccaagacggg cgagattatc agtgccgtgc
      901 acattgaggc ctacatcgga cgcgtgaccg tgtgtgccga ggccattgcc attggcagtg
      961 ccgtgagcaa cggccagaag gattttgata ccattgtggc cgtgcgtcac ccatactccg
     1021 atgaggtgga ccgtagcatc cgtgtcgtgt ccccgtgtgg aatgtgccgt gagctgatta
     1081 gcgattacgc ccccgattgc ttcgtgctga ttgagatgaa cggcaagctg gtcaagacca
     1141 ccattgagga actgattccc ctgaagtata cccgtaacta agcttggcaa taaaaagaca
     1201 gaataaaacg cacgggtgtt gggtcgtttg ttccgctgtc aggagattta ggtgacacta
     1261 tagacttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gtcgggagag
     1321 agaccaatcc atgtgggagt ttattcttga cacagatatt tatgatataa taactgagta
     1381 agcttaacat aaggaggaaa acaaaatggc gagcttggtt aagaaagata tgtgtgttaa
     1441 gatgacgatg gaaggtactg tgaacggtta tcactttaag tgcgttggcg agggtgaagg
     1501 caagccgttc gagggcacgc agaacatgcg cattcgtgtc accgagggcg gtccgctgcc
     1561 ttttgcattc gacatcctgg ccccgtgctg tatgtacggc tctaagacct tcattaaaca
     1621 cgtgagcggt atcccggatt actttaaaga gtcctttcca gagggcttca cttgggaacg
     1681 tacccagatt tttgaggacg gtggtgttct gaccgcgcac caagacacca gcctggaagg
     1741 taattgcctg atctataaag tgaaggttct gggtaccaat ttcccggcga atggtccggt
     1801 gatgcaaaag aaaaccgcgg gttgggagcc gtgcgtcgag atgctgtatc cgcgtgacgg
     1861 cgtcttgtgt ggtcagagct tgatggcgct gaagtgcacc gatggcaatc atctgaccag
     1921 ccacctgcgc acgacgtatc gtagccgtaa accgagcaac gccgttaaca tgccggagtt
     1981 ccattttggt gaccatcgca tcgaaatcct gaaagctgag cagggcaaat tctacgaaca
     2041 atacgaatcg gctgtcgcac gttacagcga tgtgccggaa aaagcgacgt aatgagcttg
     2101 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
     2161 cggttgccgc cgggcgtttt ttattggtga gaattacttc aagcttgcat gcctgcaggt
     2221 cgactctaga ggatccccgg gtaccgagct cgaattcggt ctcttcccgc ttgtgtgaaa
     2281 taccgcacag atgcgtaagg agaaaatacc gcatcaggcc tatagtgagt cgtattatcc
     2341 cgagctcgcc cggggatcga gcgcagcggt ataaaagggc gcggggtggc tgagagcatc
     2401 agttgtgaat gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa tatcctttga
     2461 ataagccaac tttgaatcac aagacgcata ccaaaccaaa tgaagctgct gagtagtatt
     2521 gaacaagcgt gtgacatatg ccgacttaag aagctgaagt gcagcaagga aaagccgaag
     2581 tgcgccaaat gtcttaagaa caattgggag tgccggtact cgccaaagac gaagcgaagc
     2641 cctttgacac gcgctcattt gactgaagtg gagagtcgcc tggagcgcct tgagcagttg
     2701 ttcctcctca tttttccccg tgaagacctc gacatgatac tgaagatgga tagtctccaa
     2761 gatattaagg ccctgctcac tggcctgttt gttcaggata acgtcaacaa ggatgccgtg
     2821 acggaccgcc ttgcctcggt ggagacagat atgccactta ccttgcggca gcatcgcatc
     2881 tcggctacca gcagtagtga agaaagttcc aataagggtc aacggcaact gacggtatcg
     2941 agtggcggag gttccggtgg cggcagcggc gggggttcgg gaggtggctc ggccaacttc
     3001 aaccagagtg gtaacattgc ggacagctcc ttgagcttca cgtttaccaa cagctccaac
     3061 ggtccaaact tgatcacgac tcagacaaac tcccaggcat tgtcccagcc cattgccagc
     3121 tcgaatgtgc atgataactt tatgaataat gaaattactg cctccaagat tgacgacggc
     3181 aacaacagca agcccctttc cccaggctgg actgaccaga cggcctacaa tgctttcggt
     3241 atcaccactg gcatgttcaa caccacaacg atggacgacg tatataacta tctttttgat
     3301 gatgaggata ctccaccgaa cccaaagaag gagtaggctt gatccagaca tgataagata
     3361 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3421 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3481 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttcgct
     3541 gtcaggagga attctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat
     3601 aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt
     3661 aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca
     3721 aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg
     3781 ccgtcacttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc
     3841 gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt
     3901 tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt
     3961 tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga
     4021 aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac
     4081 ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt
     4141 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
     4201 aaaaatccca aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag
     4261 ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca
     4321 ttcattttcg actatattct tttttacaaa aaatataaca accagatatt ttaagctgat
     4381 cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac
     4441 tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat
     4501 tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca
     4561 aaaactacgg cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc
     4621 aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc
     4681 agccgggctc cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc
     4741 gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg
     4801 gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg
     4861 cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac
     4921 cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat
     4981 gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt
     5041 ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc
     5101 catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt
     5161 gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt
     5221 gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga
     5281 tccgccgctt ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag
     5341 cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca
     5401 tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag
     5461 ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg
     5521 tgtttattaa gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc
     5581 agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg
     5641 gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca
     5701 aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc
     5761 agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt
     5821 ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga
     5881 caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa
     5941 ttcgctagat tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag
     6001 atgtatagtt tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat
     6061 gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt
     6121 gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct
     6181 gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac
     6241 atttgcattt caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct
     6301 cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact
     6361 ttctgggcaa aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg
     6421 cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg
     6481 cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca
     6541 gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct
     6601 ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc
     6661 tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc
     6721 cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc
     6781 tggagacact taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc
     6841 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
     6901 agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg
     6961 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
     7021 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
     7081 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
     7141 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
     7201 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
     7261 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
     7321 cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cggctcaata gaaattagta
     7381 ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag
     7441 ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg
     7501 agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat
     7561 gcatcgcttg acacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7621 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7681 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7741 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7801 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7861 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7921 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7981 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8041 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8101 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8161 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8221 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8281 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8341 atcaaaaagg atcttcacct agatcctttt gtgaccagcc aggacagaaa tgcctcgact
     8401 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     8461 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     8521 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     8581 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     8641 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     8701 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     8761 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     8821 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     8881 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     8941 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     9001 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     9061 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     9121 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     9181 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     9241 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     9301 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtagc tgccgactgg
     9361 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     9421 cttggacaag aagaagatcg tttggcctcg cgcgcagatc agttggaaga atttgtccac
     9481 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat cactagacca atgttacaca
     9541 tatatacttt agattgattt aaaacttcat gttacgcgcc ctgtagcggc gcattaagcg
     9601 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9661 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9721 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9781 aacttgatta gggtgatggt tcacgtagtg ggccagcgcc ctgatagacg gtttttcgcc
     9841 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9901 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9961 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
    10021 ttacaattt
//
