LOCUS       Exported                9324 bp ds-DNA     circular SYN 21-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 28, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(437..460)
                     /label=ASP15JAN01
     primer_bind     457..483
                     /label=ASP16AUG02
     primer_bind     460..483
                     /label=ASP14OCT47
     terminator      461..510
     CDS             complement(511..1521)
                     /codon_start=1
                     /gene="SpnR"
                     /label=SpmR
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1522..1655)
                     /label=Spm promoter
     primer_bind     complement(1637..1659)
                     /label=ASP14OCT46
     primer_bind     complement(1638..1659)
                     /label=ASP16AUG01
     primer_bind     1656..1681
                     /label=ASP14NOV51
     rep_origin      1694..2376
                     /direction=RIGHT
                     /label=ColE1 origin
     primer_bind     complement(2438..2461)
                     /label=ASP14NOV50
     primer_bind     2462..2485
                     /label=ASP14OCT20
     primer_bind     complement(2649..2668)
                     /label=ASP14OCT19
     primer_bind     2665..2685
                     /label=ASP14OCT18
     exon            2994..3739
                     /label=Exon6
     primer_bind     3242..3261
                     /label=ASP14OCT16
     primer_bind     complement(3380..3401)
                     /label=NSA140CT04
     intron          3740..3809
     exon            3810..3941
                     /label=Exon5
     intron          3942..4144
     exon            4145..4460
                     /label=Exon4
     primer_bind     complement(4278..4299)
                     /label=NSA140CT03
     primer_bind     complement(4424..4446)
                     /label=NSA15Jan01
                     /note="For resequencing poor region"
     intron          4461..4521
     exon            4522..5176
                     /label=Exon3
     iDNA            5177..5250
                     /label=Intron
     primer_bind     complement(5191..5214)
                     /label=NSA140CT02
     exon            5251..5524
                     /label=Exon2
     intron          5525..5925
     primer_bind     complement(5852..5874)
                     /label=NSA14OCT01
     exon            5926..6155
                     /label=Exon1
     primer_bind     complement(6224..6244)
                     /label=ASP14OCT15
     primer_bind     6240..6264
                     /label=ASP14OCT14
     primer_bind     complement(6462..6488)
                     /label=ASP14OCT13
     primer_bind     6490..6512
                     /label=ASP15APR19
     primer_bind     6490..6504
                     /label=NM19APR06
     primer_bind     6493..6512
                     /label=ASP14NOV60
     polyA_signal    6494..6689
                     /label=SV40 Late
     primer_bind     6553..6579
                     /label=ASP14NOV62
     polyA_signal    6555..6683
                     /label=SV40 Early (rev)
     primer_bind     complement(6660..6689)
                     /label=NM19APR05
     primer_bind     complement(6660..6683)
                     /label=ASPNOV61
     primer_bind     complement(6665..6693)
                     /label=ASP14NOV59
     primer_bind     6690..6716
                     /label=ASP14OCT57
     CDS             complement(6697..7413)
                     /codon_start=1
                     /label=sfGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7395..7414)
                     /label=ASP14OCT58
     primer_bind     7411..7428
                     /label=NM16NOV20
     promoter        complement(7417..7571)
                     /label=DSCP
     misc_feature    7480..7485
                     /label=DPE
                     /note="Downstream Promoter Element"
     misc_feature    7486..7495
                     /label=MTE
                     /note="Motif Ten Element"
     misc_feature    7509..7514
                     /label=Inr
                     /note="Inititator"
     TATA_signal     7538..7543
                     /label=TATA
     primer_bind     complement(7556..7571)
                     /label=NM17JAN03
     primer_bind     complement(7556..7571)
                     /label=NM16NOV19
     protein_bind    7578..7593
                     /bound_moiety="QF"
     protein_bind    7596..7611
                     /bound_moiety="QF"
     protein_bind    7616..7627
                     /bound_moiety="QF"
     protein_bind    7632..7647
                     /bound_moiety="QF"
     protein_bind    7650..7665
                     /bound_moiety="QF"
     misc_feature    7678..7725
                     /label=HSV-TK
     primer_bind     7678..7700
                     /label=NM18AUG02
     primer_bind     7726..7751
                     /label=Mock NM15SEP13
     CDS             complement(7730..8152)
                     /codon_start=1
                     /product="Blasticidin S Resistance Gene (bsr). Produces 
                     Blasticidin S Deaminase and confers resistance to 
                     Blasticidin in prokaryotes and eukaryotes. BS is a 
                     nucleoside antibiotic that inhibits protein synthesis. "
                     /label=bsr
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     primer_bind     complement(8132..8153)
                     /label=Mock NM15SEP12
     primer_bind     8150..8171
                     /label=NM15OCT02
     misc_feature    8154..8156
                     /label=Kozak
     mRNA            complement(8161..8166)
                     /label=SD
     promoter        complement(8167..8234)
                     /label=CP6 Promoter
     primer_bind     complement(8214..8238)
                     /label=ASP14SEP09
     primer_bind     8232..8264
                     /label=NM16NOV21
     promoter        complement(8239..8693)
                     /label=Hsp70B
     primer_bind     8239..8264
                     /label=ASP14NOV42
     primer_bind     complement(8563..8584)
                     /label=ASP14NOV41
     primer_bind     8580..8605
                     /label=ASP14NOV40
     primer_bind     complement(8672..8700)
                     /label=ASP14NOV39
     primer_bind     complement(8673..8693)
                     /label=NM18AUG01
     primer_bind     complement(8684..8700)
                     /label=NM16NOV22
     primer_bind     8702..8730
                     /label=ASP16SEP18
     primer_bind     8702..8730
                     /label=ASP16SEP16
     primer_bind     8705..8725
                     /label=ASP14NOV46
     protein_bind    complement(8706..8817)
                     /label=attB_TT
                     /bound_moiety="phage phi-C31 integrase"
     misc_feature    complement(8739..8772)
                     /label=attB_TT
     primer_bind     complement(8781..8817)
                     /label=ASP14JUL13
     primer_bind     complement(8796..8821)
                     /label=ASP16SEP17
     primer_bind     complement(8796..8821)
                     /label=ASP16SEP15
     primer_bind     complement(8798..8817)
                     /label=ASP14NOV45
     primer_bind     8824..8857
                     /label=ASP15MAR25
     CDS             complement(8935..9222)
                     /codon_start=1
                     /label=LacZ
                     /translation="MTMITPSYLGDTIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGE"
     primer_bind     9075..9091
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9098..9116
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    9119..9175
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(9182..9200)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(9254..9301)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(9296..9312)
                     /label=ASP15MAR27
     primer_bind     complement(9298..9324)
                     /label=ASP15MAR13
     primer_bind     join(9320..9324,1..32)
                     /label=ASP15JUL28
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgctggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtaaca tgaagtttta aatcaatcta
      481 aagtatatat gtgtaacatt ggtctagtga ttatttgccg actaccttgg tgatctcgcc
      541 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaaac gatcttcttc
      601 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
      661 ctccattgcc cagtcggcag ctacatcctt cggcgcgatt ttgccggtta ctgcgctgta
      721 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
      781 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
      841 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
      901 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
      961 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
     1021 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
     1081 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
     1141 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
     1201 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
     1261 tgatagttga gtcgatactt cggcgatcac cgcttccctc ataatgttta actttgtttt
     1321 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     1381 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
     1441 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     1501 ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta
     1561 tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
     1621 tagcagcgaa gtcgaggcat ttctgtcctg gctggtcaca aaaggatcta ggtgaagatc
     1681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgtc
     2461 aagcgatgca tctacacaag gaacaaacac tggatgtcac tttcagttca aattgtaacg
     2521 ctaatcactc cgaacaggtc acaaaaaatt accttaaaaa gtcataatat taaattagaa
     2581 taaatatagc tgtgagggaa atatatacaa atatattgga gcaaataaat tgtacataca
     2641 aatatttatt actaatttct attgagccga aatgaaccac tcggaaccat ttgagcgaac
     2701 cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt
     2761 gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg gtggaggtga
     2821 attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc ccggtgtttt
     2881 atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga
     2941 agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt attctatagg
     3001 tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg gggcaataaa
     3061 cagtaaacac gatggtaata atggtaaaaa aaaaaaacaa gcagttattt cggatatatg
     3121 tcggctactc cttgcgtcgg gcccgaagtc ttagagccag atatgcgagc acccggaagc
     3181 tcacgatgag aatggccaga cccacgtagt ccagcggcag atcggcggcg gagaagttaa
     3241 gtgtctccag gatgaccttg cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa
     3301 tttcgcccgg ctccacgtcc gcccattggt taatcagcag accctcgttg gcgtaacgga
     3361 accatgagag gtacgacaac catttgaggt atactggcac cgagcccgag ttcaagaaga
     3421 agccgccaaa gagcaggaat ggtatgataa ccggcggacc cacagacagc gccatcgagg
     3481 tcgaggagct ggcgcaggat attagatatc cgaaggacgt tgacacattg gccaccagag
     3541 tgaccagcgc caggcagttg aagaagtgca gcactccggc ccgcagtccg atcatcggat
     3601 aggcaatcgc cgtgaagacc agtggcactg tgagaaaaag cggcaattcg gcaatcgttt
     3661 tgcccagaaa gtatgtgtca cagcgataaa gtcgacttcg ggcctccctc ataaaaactg
     3721 gcagctctga ggtgaacacc taaatcgaat cgattcatta gaaagttagt aaattattga
     3781 aatgcaaatg tattctaaac atgacttaca tttatcgtgg caaagacgtt ttgaaaggtc
     3841 atgttggtca ggaagaggaa gatggctccg ttgatattca tcacacccac ttgcgtgagt
     3901 tgttggccca aaaagatgag gccaatcaag atggcaacca tctgcaaatt aaaatgttac
     3961 tcgcatctca ttaatattcg cgagttaaat gaaatttatt tatcttctgc aaaactataa
     4021 actatacatc tcattgaaaa aaactaagaa gggtgtggaa tcaggcaatt ctatctaaaa
     4081 tctagcgaat ttgtttccaa gaattgtaag cgttatatca tttgtttcca ctggaaccac
     4141 tcaccgttgt ctgaataagt cgcactttta cgaggagtgg ttccttgagc accgacagcc
     4201 aggatcgcca caggaccgcc cggaactgca tgaaccaggt ggccttgtag gtgtacccat
     4261 tctccggctg ctccagtggc ttctccagat ttttggtggc caacaactgc tccatatccc
     4321 gggctacttt gctaatggca aaattgtcgc atatcttggc gatccgatca cgggactcga
     4381 tctcccgtcc gggcacaacg gccaacacct gtacgtaaaa gtccgccgga ttgtagttgg
     4441 taggacactg ggcacccacg ctggatagga gttgagatgt tatgtaatac tagataccct
     4501 taataaacac atcgaactca ctaggaaaag aagtcgacgg cttcgctggg agtgcccaag
     4561 aaagctaccc tgccctcggc catcagaagg atcttgtcaa agagctcaaa cagctcggaa
     4621 gacggctgat gaatggtcag gatgacggtc ttgcccttct gcgacagctt cttcagcacc
     4681 tggacgacgc tgtgggcggt aaaggagtcc agtccggagg tgggctcatc gcagatcaga
     4741 agcggcggat cggttagagc ctcggaggcg aatgccagac gcttcctttc tccgccggac
     4801 agacctttca ccctgccggg cacaccgatg atcgtgtgct gacatttgct gagcgaaagc
     4861 tcctggatca cctgatccac gcgggccact cgctgccgat aggtcagatg tcgtggcatc
     4921 cgcaccatgg cttggaaaat caggtgttcc ctggccgtta gggagccgat aaagaggtca
     4981 tcctgctgga cataggcgca cctggcctgc atctccttgg cgtccacagg ttggccattg
     5041 agcagtcgca tcccggatgg cgatacttgg atgccctgcg gcgatcgaaa ggcaagggca
     5101 ttcagcaggg tcgtctttcc ggcaccggaa ctgcccatca cggccaaaag ttcgcccgga
     5161 taggccacgc cgcaaactga gtttcaaatt ggtaattgga ccctttatta agatttcaca
     5221 cagatcagcc gactgcgaat agaaactcac cgttcttgag caaatgtttc ctgggcgccg
     5281 gtatgtgtcg ctcgttgcag aatagtccgc gtgtccggtt gaccagctgc cgccatccgg
     5341 agcccggctg attgaccgcc ccaaagatgt ccatattgtg ccaggcatag gtgaggttct
     5401 cggctagttg gccgctccct gaaccggagt cctccggcgg actgggtggc cggagcgtgc
     5461 cgtagttttt ggcctgcccg aagccctggt taatgcagct ctgcgaagcc gctccgctgt
     5521 caccctgcaa tgatagggga tctcaaatat caactacaag cgttatgctc atctaacccc
     5581 gaacaaaaag taccccgaag tatcctacga agtaggttta tacttttatt tattttttgt
     5641 gcatctagga tcagcttaaa atatctggtt gttatatttt ttgtaaaaaa gaatatagtc
     5701 gaaaatgaat gcctttagat gtcttgatca tgatatgatc tcaaaaattg tcttatatag
     5761 cgagaacagc taccagaata atctgtttcg tgtcactatt tgtttgtgca attgcggttt
     5821 gggatttttg tgggtcgcag ttctcacgcc gcagacaatt tgatgttgca atcgcagttc
     5881 ctatagatca agtgaactta agatgtatgc acatgtacta ctcacattgt tcagatgctc
     5941 ggcagatggg tgtttgctgc ctccgcgaat taatagctcc tgatcctctt ggcccattgc
     6001 cgggattttt cacactttcc cctgcttacc cacccaaaac caatcaccac cccaatcact
     6061 caaaaaacaa acaaaaataa gaagcgagag gagttttggc acagcacttt gtgtttaatt
     6121 gatggcgtaa accgcttgga gcttcgtcac gaaaccgctg acaaaatgca actgaaggcg
     6181 gacattgacg ctacgtaacg ctacaaacgg tggcgaaaga gatagcggac gcagcggcga
     6241 aagtgacggc gatatttctg tggacagaga aggaggcaaa cagcgctgac tttgagtgga
     6301 atgtcatttt gagtgagagg taatcgaaag aacctggtac atcaaatacc cttggatcga
     6361 agtaaattta aaactgatca gataagttca atgatatcca gtgcagtaaa aaaaaaaaat
     6421 gtttttttta tctactttcc gcaaaaatgg gttttattaa cttacataca tactagaatt
     6481 cctcctgaca gcgaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     6541 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6661 tcatcaatgt atcttatcat gtctggatca agcttacttg tacagctcat ccatgcccag
     6721 ggtgatgccg gcggcggtca cgaactccag cagcaccatg tgatcgcgct tctcgttggg
     6781 atccttggac agcacgctct gggtgctcag gtagtggtta tcgggcagca gcactgggcc
     6841 gtcgccgatg ggggtgttct gctggtagtg atcggccagc tgcacggagc catcctccac
     6901 attgtggcgg atcttgaagt tggccttgat gccgttcttc tgcttatcgg cggtgatgta
     6961 cacgttgtgg ctgttgaagt tgtactccag cttgtggccc aggatgttgc catcctcctt
     7021 gaaatcgatg cccttcagct cgatgcggtt caccagggta tcgccctcga acttcacctc
     7081 ggcgcgggtc ttgtaggtgc cgtcatcctt gaagctgatg gtgcgctcct gcacgtagcc
     7141 ctcgggcatg gcgctcttga agaaatcgtg ctgcttcatg tgatcggggt agcggctgaa
     7201 gcactgcacg ccgtaggtca gggtggtcac cagggtgggc cagggcacgg gcagcttgcc
     7261 ggtggtgcag atgaacttca gggtcagctt gccgttggtg gcgtcgccct cgccctcgcc
     7321 gcgcacgctg aacttgtggc cgttcacgtc gccatccagc tccaccagga tgggcaccac
     7381 gccggtgaac agctcctcgc ccttggacac catttggttt ggtatgcgtc ttgtgattca
     7441 aagttggctt attcaaagga tattaacgaa ggcagcggca cgtctgctcg gctcgaacat
     7501 tcattcacaa ctgatgctct cagccacccc gcgccctttt ataccgctgc gctcgatccc
     7561 cgggcgagct cgggaaagga taagcgatta cccgaggata agcgattacc cggataagcg
     7621 attacccgtg aggataattg tttatccgag gataagcgat tacccctcct gacagcggaa
     7681 caaacgaccc aacacccgtg cgttttattc tgtcttttta ttgccaagct tagttacggg
     7741 tatacttcag gggaatcagt tcctcaatgg tggtcttgac cagcttgccg ttcatctcaa
     7801 tcagcacgaa gcaatcgggg gcgtaatcgc taatcagctc acggcacatt ccacacgggg
     7861 acacgacacg gatgctacgg tccacctcat cggagtatgg gtgacgcacg gccacaatgg
     7921 tatcaaaatc cttctggccg ttgctcacgg cactgccaat ggcaatggcc tcggcacaca
     7981 cggtcacgcg tccgatgtag gcctcaatgt gcacggcact gataatctcg cccgtcttgg
     8041 tgcgaatggc agctcccacg tggtgcttgt tatcctcgta gagcatcgta atcttctcgg
     8101 tagccacctc caccagttcc aggtcctgct ggctgatgtt gaaggttttc attttgtttt
     8161 cctccttatg ttaagcttac tcagttatta tatcataaat atctgtgtca agaataaact
     8221 cccacatgga ttcgagtatt gagagttctc ttcttgtatt caataattac ttcttggcag
     8281 atttcagtag ttgcagttga tttacttggt tgctggttac ttttaattga ttcactttaa
     8341 cttgcacttt actgcagatt gtttagcttg ttcagctgcg cttgtttatt tgcttagctt
     8401 tcgcttagcg acgtgttcac tttgcttgtt tgaattgaat tgtcgctccg tagacgaagc
     8461 gcctctattt atactccggc gctcttttcg cgaacattcg aggcgcgctc tctcgaacca
     8521 acgagagcag tatgccgttt actgtgtgac agagtgagag agcattagtg cagagaggga
     8581 gcgacccaaa aagaaaagag agaataacga ataacggcca gagaaatttc tcgagttttc
     8641 tttctgccaa acaaatgacc taccacaata accagtttgt tttgggattc tagctcctga
     8701 cagcgcatca tgatggacca gatgggtgag gtggagtacg cgcccgggga gcccaagggc
     8761 acgccctggc acccgcaccg cggcttcgag acagtgacct acatcgtcga ccaattcctc
     8821 cctgggatcc ggagagagac cctatgcggc atcagagcag attgtactga gagtgcacca
     8881 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgtcattc
     8941 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     9001 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     9061 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
     9121 attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca agcttgagta
     9181 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atgatctgcc tcctgggttt
     9241 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
     9301 caggggtctc tcgctgatcc gtta
//
