LOCUS       pATP416__ptetO7_       12338 bp ds-DNA     circular     28-FEB-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Tominaga M et al Robust and flexible platform for directed
            evolution of yeast genetic switches.
COMMENT     Sequence conflict with pRS416 backbone (genbank U03450) are
            highlighted.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      11767..12274
                     /locus_tag="CEN6_ARS4"
                     /label="CEN6_ARS4"
                     /ApEinfo_label="CEN6_ARS4"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1344..1799)
                     /locus_tag="M13 origin"
                     /label="M13 origin"
                     /ApEinfo_label="M13 origin"
                     /ApEinfo_fwdcolor="#bfbfbf"
                     /ApEinfo_revcolor="#bfbfbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1..186
                     /organism="Cloning vector pRS416"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31841"
                     /locus_tag="source:Cloning vector pRS416"
                     /label="source:Cloning vector pRS416"
                     /ApEinfo_label="source:Cloning vector pRS416"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     enhancer        complement(4656..4901)
                     /locus_tag="Tet Response element"
                     /label="Tet Response element"
                     /ApEinfo_label="Tet Response element"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(2400..4421)
                     /locus_tag="CrtYB"
                     /label="CrtYB"
                     /ApEinfo_label="CrtYB"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7924..7953
                     /locus_tag="phlO"
                     /label="phlO"
                     /ApEinfo_label="phlO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     conflict        4356..4356
                     /locus_tag="C66A"
                     /label="C66A"
                     /ApEinfo_label="C66A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     conflict        complement(3063..3063)
                     /locus_tag="C1359T"
                     /label="C1359T"
                     /ApEinfo_label="C1359T"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      complement(2092..2391)
                     /locus_tag="ADH1 terminator"
                     /label="ADH1 terminator"
                     /ApEinfo_label="ADH1 terminator"
                     /ApEinfo_fwdcolor="#aba19a"
                     /ApEinfo_revcolor="#aba19a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1910..2064
                     /organism="Cloning vector pRS416"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31841"
                     /locus_tag="source:Cloning vector pRS416(1)"
                     /label="source:Cloning vector pRS416(1)"
                     /ApEinfo_label="source:Cloning vector pRS416"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     protein_bind    complement(4878..4896)
                     /locus_tag="tetO"
                     /label="tetO"
                     /ApEinfo_label="tetO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7860..7889
                     /locus_tag="phlO(1)"
                     /label="phlO(1)"
                     /ApEinfo_label="phlO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1890..1909
                     /organism="Cloning vector pRS416"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31841"
                     /locus_tag="source:Cloning vector pRS416(2)"
                     /label="source:Cloning vector pRS416(2)"
                     /ApEinfo_label="source:Cloning vector pRS416"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     protein_bind    7796..7825
                     /locus_tag="phlO(2)"
                     /label="phlO(2)"
                     /ApEinfo_label="phlO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1939..1956
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1476..1782)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7892..7921
                     /locus_tag="phlO(3)"
                     /label="phlO(3)"
                     /ApEinfo_label="phlO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1800..1868)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(4842..4860)
                     /locus_tag="tetO(1)"
                     /label="tetO(1)"
                     /ApEinfo_label="tetO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          11428..12275
                     /organism="Cloning vector pRS416"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31841"
                     /locus_tag="source:Cloning vector pRS416(3)"
                     /label="source:Cloning vector pRS416(3)"
                     /ApEinfo_label="source:Cloning vector pRS416"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     conflict        12274..12274
                     /locus_tag="CC to C (C deleted)"
                     /label="CC to C (C deleted)"
                     /ApEinfo_label="CC to C (C deleted)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7764..7793
                     /locus_tag="phlO(4)"
                     /label="phlO(4)"
                     /ApEinfo_label="phlO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(9626..9648)
                     /locus_tag="lacO"
                     /label="lacO"
                     /ApEinfo_label="lacO"
                     /ApEinfo_fwdcolor="#00a5d8"
                     /ApEinfo_revcolor="#00a5d8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(4428..4655)
                     /locus_tag="pGAL1-c"
                     /label="pGAL1-c"
                     /ApEinfo_label="pGAL1-c"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7828..7857
                     /locus_tag="phlO(5)"
                     /label="phlO(5)"
                     /ApEinfo_label="phlO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     conflict        9659..9659
                     /locus_tag="G to C"
                     /label="G to C"
                     /ApEinfo_label="G to C"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4972..5671
                     /locus_tag="TDH3 promoter"
                     /label="TDH3 promoter"
                     /ApEinfo_label="TDH3 promoter"
                     /ApEinfo_fwdcolor="#858fb6"
                     /ApEinfo_revcolor="#a9b6d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(4807..4825)
                     /locus_tag="tetO(2)"
                     /label="tetO(2)"
                     /ApEinfo_label="tetO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(4771..4789)
                     /locus_tag="tetO(3)"
                     /label="tetO(3)"
                     /ApEinfo_label="tetO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        7967..8194
                     /locus_tag="pGAL1-c(1)"
                     /label="pGAL1-c(1)"
                     /ApEinfo_label="pGAL1-c"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(9824..10764)
                     /locus_tag="pUC origin"
                     /label="pUC origin"
                     /ApEinfo_label="pUC origin"
                     /ApEinfo_fwdcolor="#bfbfbf"
                     /ApEinfo_revcolor="#bfbfbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(4735..4753)
                     /locus_tag="tetO(4)"
                     /label="tetO(4)"
                     /ApEinfo_label="tetO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(4664..4682)
                     /locus_tag="tetO(5)"
                     /label="tetO(5)"
                     /ApEinfo_label="tetO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(4700..4718)
                     /locus_tag="tetO(6)"
                     /label="tetO(6)"
                     /ApEinfo_label="tetO"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1800..1889
                     /organism="Cloning vector pRS416"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31841"
                     /locus_tag="source:Cloning vector pRS416(4)"
                     /label="source:Cloning vector pRS416(4)"
                     /ApEinfo_label="source:Cloning vector pRS416"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     primer_bind     1966..1984
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#f0abff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1234..1343
                     /organism="Cloning vector pRS416"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31841"
                     /locus_tag="source:Cloning vector pRS416(5)"
                     /label="source:Cloning vector pRS416(5)"
                     /ApEinfo_label="source:Cloning vector pRS416"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     conflict        1353..1353
                     /locus_tag="A to G"
                     /label="A to G"
                     /ApEinfo_label="A to G"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      7435..7734
                     /locus_tag="TDH3 terminator"
                     /label="TDH3 terminator"
                     /ApEinfo_label="TDH3 terminator"
                     /ApEinfo_fwdcolor="#aba19a"
                     /ApEinfo_revcolor="#aba19a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5678..7426
                     /locus_tag="CrtI"
                     /label="CrtI"
                     /ApEinfo_label="CrtI"
                     /ApEinfo_fwdcolor="#0000a0"
                     /ApEinfo_revcolor="#0000a0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6958..6958
                     /locus_tag="C1281A"
                     /label="C1281A"
                     /ApEinfo_label="C1281A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(10768..11427)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7375..7375
                     /locus_tag="G1698A"
                     /label="G1698A"
                     /ApEinfo_label="G1698A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      9223..9510
                     /locus_tag="PGK1 terminator"
                     /label="PGK1 terminator"
                     /ApEinfo_label="PGK1 terminator"
                     /ApEinfo_fwdcolor="#aba19a"
                     /ApEinfo_revcolor="#aba19a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          8201..9208
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4932"
                     /locus_tag="source:Saccharomyces cerevisiae"
                     /label="source:Saccharomyces cerevisiae"
                     /ApEinfo_label="source:Saccharomyces cerevisiae"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     CDS             8201..9208
                     /gene="BTS1"
                     /note="geranylgeranyl diphosphate synthase"
                     /codon_start=1
                     /product="Bts1p"
                     /protein_id="AAA83262.1"
                     /db_xref="GI:1098641"
                     /translation="MEAKIDELINNDPVWSSQNESLISKPYNHILLKPGKNFRLNLIV
                     QINRVMNLPKDQLAIVSQIVELLHNSSLLIDDIEDNAPLRRGQTTSHLIFGVPSTINT
                     ANYMYFRAMQLVSQLTTKEPLYHNLITIFNEELINLHRGQGLDIYWRDFLPEIIPTQE
                     MYLNMVMNKTGGLFRLTLRLMEALSPSSHHGHSLVPFINLLGIIYQIRDDYLNLKDFQ
                     MSSEKGFAEDITEGKLSFPIVHALNFTKTKGQTEQHNEILRILLLRTSDKDIKLKLIQ
                     ILEFDTNSLAYTKNFINQLVNMIKNDNENKYLPDLASHSDTATNLHDELLYIIDHLSE
                     L"
                     /locus_tag="Bts1p"
                     /label="Bts1p"
                     /ApEinfo_label="Bts1p"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     gene            8201..9208
                     /gene="BTS1"
                     /locus_tag="BTS1"
                     /label="BTS1"
                     /ApEinfo_label="BTS1"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          9523..9625
                     /organism="Cloning vector pRS416"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31841"
                     /locus_tag="source:Cloning vector pRS416(6)"
                     /label="source:Cloning vector pRS416(6)"
                     /ApEinfo_label="source:Cloning vector pRS416"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     source          10765..11427
                     /organism="Cloning vector pRS416"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31841"
                     /locus_tag="source:Cloning vector pRS416(7)"
                     /label="source:Cloning vector pRS416(7)"
                     /ApEinfo_label="source:Cloning vector pRS416"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     conflict        9706..9706
                     /locus_tag="G to C(1)"
                     /label="G to C(1)"
                     /ApEinfo_label="G to C"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     conflict        10447..10447
                     /locus_tag="G to A"
                     /label="G to A"
                     /ApEinfo_label="G to A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            187..1233
                     /locus_tag="URA3 marker (pRS)"
                     /label="URA3 marker (pRS)"
                     /ApEinfo_label="URA3 marker (pRS)"
                     /ApEinfo_fwdcolor="#cceaf2"
                     /ApEinfo_revcolor="#cceaf2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     conflict        240..240
                     /locus_tag="T to C"
                     /label="T to C"
                     /ApEinfo_label="T to C"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     conflict        190..195
                     /locus_tag="agcttttcaa to ctttt"
                     /label="agcttttcaa to ctttt"
                     /ApEinfo_label="agcttttcaa to ctttt"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcC
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aaGcgtTAAT ATTTTgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaaGC CGGCGAACGT
     1681 GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC
     1741 GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
     2041 gaattcctgc agcccggggg atccactagt ggtaccgggc cccccCTCGA Gttgtcctct
     2101 gaggacataa aatacacacc gagattcatc aactcattgc tggagttagc atatctacaa
     2161 ttgggtgaaa tggggagcga tttgcaggca tttgctcggc atgccggtag aggtgtggtc
     2221 aataagagcg acctcatgct atacctgaga aagcaacctg acctacagga aagagttact
     2281 caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat
     2341 tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg cGGCCGGCCt
     2401 tactgccctt cccatccgct catgaccaca ctcaagactt tccgtactct cctccatccg
     2461 gcaactgtcc ttctctctcc gacgtctcct ttccaaacga ctttgatctc tcggccgatc
     2521 agtaggtagc tcgcgcaagc cgctcgcatt cccgcttgaa cctcggtagg aagtcggtca
     2581 attcccttat aagaatgttt ggcaagatcc tctgcgtagg cgactaatgg aagcgagtac
     2641 gtcttccatt cgaaccggaa gctttctgag gcgtttgaag atggtaatgt ggacgaagga
     2701 gatagactga ggagtttgtc gaaatcttga ggccgaggtt ccgtccaatc agtcgggatc
     2761 gcaagctttg attcatcccg aagaccaaag aatgagagtg gtaggtaaaa tctcccttct
     2821 gttgcgtccc ctttaatgtc cctagcaatg ttcaccaact gaagggcagt tcccatctct
     2881 cggcttgcca ctaacacagc ttctctttct tctatggtgg cagggacctg acttggtgca
     2941 cttgcccaag agacatagac caatagctcg gcgactgagc ctgctacaca tagaccatag
     3001 tccagcaagt cagctgtggt ctcgataggc gtcttccgag cctggactgc ctctgtcgat
     3061 aaAggaaaga taagatcagt ggtgtatcct ctaaggagtt cgtcgagtgg gtatcgaggg
     3121 atcagccctt gcaacttagc gagcaacctg aaggcgaaat ggtattgaac gggaaccctt
     3181 tcggtaagga attgaacgag ctcggcaggc gagagcgaag gaggaggcgg gaggggatac
     3241 attcccgtgg gtcgggaagg gtgcgaagga ggaagtaaag gcgaagaaag gatcttgtca
     3301 ggttgcgaag ggtgtagcgg gggcccaaat agtagggtaa gaaaatcgga gaccatgtca
     3361 attgtggcat gcgggttgga agatacttca ggagagtcga taagatcatc agtcacccgg
     3421 cagaatgcgt atagtccaac cagcctctcc ctaacttcgc taggaaatcc agccgaggca
     3481 acaaaaaagc tccggctctt ttcctccaac aacttgactg ccagttccaa gtcacgtttt
     3541 ggctgagaag agtatggtcg gctgctaaaa aacagggaga gcacaggcgg tgtaatgagg
     3601 ggaaatgaag atggcatctt tttgttgcca taaatagttc gaccgtgtag caggtatagg
     3661 gcctgagtat gatcgcaggc agacagaccc agaacaatca ttagattcgt cagtaagaag
     3721 aacatagctt cctcaatggg tagtacacct ccaagcctcc accctacaat cttctcatcg
     3781 ttgatcgacc aagagtcttg accgacagca acataatcta cccaaatcag atacaccgtc
     3841 gggatcatga ttgctgcaat agttgacttt gctcggccac ttttccaatc gaaagcatat
     3901 tcgcctgata atgctgccaa gagcatggta ggtggggtga tgagtaagga gagtgcccgc
     3961 atgtagaagt agtgatctgt cacgagcggg tcgggcgatg ggctggggtg agcggtaaat
     4021 aggtagataa tgggcagagg gatgagcgcc ttgagcgcga gagaaagggc ggacgatcta
     4081 gtcttgggaa gcgcgagaga tgggagaagg tgcctagttg ccaagacgta gaccaagccg
     4141 gtgattacgg tttgaatgac aaagaaagcg tactcttcat atggaacatc tagaaacgtt
     4201 ccaaacacgc cttggccact ctccgctgat ggatatgtcc atgcgccatt tctgatgatc
     4261 catgagtccc atggtgtggt tgcactaaac gcaataaata cgaggatcga tattttgtag
     4321 atgtcaaatt ttgtcaaaat cggggaagtg agcagAccga gaagaccaag aattgggaga
     4381 gtatagatca gatggatctg gtaataTGCG AGAGCCGTCA TCCTAGGtat agttttttct
     4441 ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt tattacattt
     4501 gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagtt
     4561 tttgcattta tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt aattacccca
     4621 gaaataaggc taaaaaacta atcgcattat catccacata cgtTCTCTAT CACTGATAGG
     4681 GAgtaaactc gacatacgtT CTCTATCACT GATAGGGAta aactcgacat acgtTCTCTA
     4741 TCACTGATAG GGAgtaaact cgacatacgt TCTCTATCAC TGATAGGGAg taaactcgac
     4801 atacgtTCTC TATCACTGAT AGGGAgtaaa ctcgacatcg tTCTCTATCA CTGATAGGGA
     4861 gtaaactcga catacgtTCT CTATCACTGA TAGGGAgtaa aCTCGAGGGG GGGCCCGGTA
     4921 CCCAATTCGC CCTATAGTGA GTCGGGATcc gctggcgtaa tagcgcttaa ggaataaaaa
     4981 acacgctttt tcagttcgaG tttatcatta tcaatactgc catttcaaag aatacgtaaa
     5041 taattaatag tagtgatttt cctaacttta tttagtcaaa aaattagcct tttaattctg
     5101 ctgtaacccg tacatgccca aaataggggg cgggttacac agaatatata acatcgtagg
     5161 tgtctgggtg aacagtttat tcctggcatc cactaaatat aatggagccc gctttttaag
     5221 ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat tgttttcttc accaaccatc
     5281 agttcatagg tccattctct tagcgcaact acagagaaca ggggcacaaa caggcaaaaa
     5341 acgggcacaa cctcaatgga gtgatgcaac ctgcctggag taaatgatga cacaaggcaa
     5401 ttgacccacg catgtatcta tctcattttc ttacaccttc tattaccttc tgctctctct
     5461 gatttggaaa aagctgaaaa aaaaggttga aaccagttcc ctgaaattat tcccctactt
     5521 gactaataag tatataaaga cggtaggtat tgattgtaat tctgtaaatc tatttcttaa
     5581 acttcttaaa ttctactttt atagttagtc ttttttttag ttttaaaaca ccaagaaCtt
     5641 agtttcgaat aaacacacat aaacaaacaa aacgcgtatg ggaaaagaac aagatcagga
     5701 taaacccaca gctatcatcg tgggatgtgg tatcggtgga atcgccactg ccgctcgtct
     5761 tgctaaagaa ggtttccagg tcacggtgtt cgagaagaac gactactccg gaggtcgatg
     5821 ctctttaatc gagcgagatg gttatcgatt cgatcagggg cccagtttgc tgctcttgcc
     5881 agatctcttc aagcagacat tcgaagattt gggagagaag atggaagatt gggtcgatct
     5941 catcaagtgt gaacccaact atgtttgcca cttccacgat gaagagactt tcactctttc
     6001 aaccgacatg gcgttgctca agcgggaagt cgagcgtttt gaaggcaaag atggatttga
     6061 tcggttcttg tcgtttatcc aagaagccca cagacattac gagcttgctg tcgttcacgt
     6121 cctgcagaag aacttccctg gcttcgcagc attcttacgg ctacagttca ttggccaaat
     6181 cctggctctt caccccttcg agtctatctg gacaagagtt tgtcgatatt tcaagaccga
     6241 cagattacga agagtcttct cgtttgcagt gatgtacatg ggtcaaagcc catacagtgc
     6301 gcccggaaca tattccttgc tccaatacac cgaattgacc gagggcatct ggtatccgag
     6361 aggaggcttt tggcaggttc ctaatactct tcttcagatc gtcaagcgca acaatccctc
     6421 agccaagttc aatttcaacg ctccagtttc ccaggttctt ctctctcctg ccaaggaccg
     6481 agcgactggt gttcgacttg aatccggcga ggaacatcac gccgatgttg tgattgtcaa
     6541 tgctgacctc gtttacgcct ccgagcactt gattcctgac gatgccagaa acaagattgg
     6601 ccaactgggt gaagtcaaga gaagttggtg ggctgactta gttggtggaa agaagctcaa
     6661 gggaagttgc agtagtttga gcttctactg gagcatggac cgaatcgtgg acggtctggg
     6721 cggacacaat atcttcttgg ccgaggactt caagggatca ttcgacacaa tcttcgagga
     6781 gttgggtctc ccagccgatc cttcctttta cgtgaacgtt ccctcgcgaa tcgatccttc
     6841 tgccgctccc gaaggcaaag atgctatcgt cattcttgtg ccgtgtggcc atatcgacgc
     6901 ttcgaaccct caagattaca acaagcttgt tgctcgggca aggaagtttg tgatccaAac
     6961 gctttccgcc aagcttggac ttcccgactt tgaaaaaatg attgtggcag agaaggttca
     7021 cgatgctccc tcttgggaga aagaattcaa cctcaaggac ggaagcatct tgggactggc
     7081 tcacaacttt atgcaagttc ttggtttcag gccgagcacc agacatccca agtatgacaa
     7141 gttgttcttt gtcggggctt cgactcatcc cggaactggg gttcccatcg tcttggctgg
     7201 agccaagtta actgccaacc aagttctcga atcctttgac cgatccccag ctccagatcc
     7261 caatatgtca ctctccgtac catatggaaa acctctcaaa tcaaatggaa cgggtatcga
     7321 ttctcaggtc cagctgaagt tcatggattt ggagagatgg gtataccttt tggtAttgtt
     7381 gattggggcc gtgatcgctc gatccgttgg tgttcttgct ttctgagcgg ccgcgtgaat
     7441 ttactttaaa tcttgcattt aaataaattt tctttttata gctttatgac ttagtttcaa
     7501 tttatatact attttaatga cattttcgat tcattgattg aaagctttgt gttttttctt
     7561 gatgcgctat tgcattgttc ttgtcttttt cgccacatgt aatatctgta gtagatacct
     7621 gatacattgt ggatgctgag tgaaatttta gttaataatg gaggcgctct taataatttt
     7681 ggggatattg gctttttttt ttaaagttta caaatgaATT TTTTCCGCCA GGATTTAatt
     7741 aacgctatta cgccagcgga tccATGATAC GAAACGTACC GTATCGTTAA GGTGAATGAT
     7801 ACGAAACGTA CCGTATCGTT AAGGTCAATG ATACGAAACG TACCGTATCG TTAAGGTCAA
     7861 TGATACGAAA CGTACCGTAT CGTTAAGGTG TATGATACGA AACGTACCGT ATCGTTAAGG
     7921 TAGATGATAC GAAACGTACC GTATCGTTAA GGTggcgttg tgatccggat gataatgcga
     7981 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
     8041 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
     8101 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
     8161 ctctatactt taacgtcaag gagaaaaaac tataGCTAGC ATGGAGGCCA agatagatga
     8221 gCtgatcaat aatgatcctg tttggtccag ccaaaatgaa agcttgattt caaaacctta
     8281 taatcacatc cttttgaaac ctggcaagaa ctttagacta aatttaatag ttcaaattaa
     8341 cagagttatg aatttgccca aagaccagct ggccatagtt tcgcaaattg ttgagctctt
     8401 gcataattcc agccttttaa tcgacgatat agaagataat gctcccttgA GAAGGGGACA
     8461 GACCACTTCt cacttaatct tcggtgtacc ctccactata aacaccgcaa attatatgta
     8521 tttcagagcc atgcaacttg tatcgcagct aaccacaaaa gagcctttgt atcataattt
     8581 gattacgatt ttcaacgaag aattgatcaa tctacatagg ggacaaggct tggatatata
     8641 ctggagagac tttctgcctg aaatcatacc tactcaggag atgtatttga atatggttat
     8701 gaataaaaca ggcggccttt tcagattaac gttgagactc atggaagcgc tgtctccttc
     8761 ctcacaccac ggccattcgt tggttccttt cataaatctt ctgggtatta tttatcagat
     8821 tagagatgat tacttgaatt tgaaagattt ccaaatgtcc agcgaaaaag gctttgctga
     8881 ggacattaca gaggggaagt tatcttttcc catcgtccac gcccttaact tcactaaaac
     8941 gaaaggtcaa actgagcaac acaatgaaat tctaagaatt ctcctgttga ggacaagtga
     9001 taaagatata aaactaaagc tgattcaaat actggaattc gacaccaatt cattggccta
     9061 caccaaaaat tttattaatc aattagtgaa tatgataaaa aatgataatg aaaataagta
     9121 tttacctgat ttggcttcgc attccgacac cgccaccaat ttacatgacg aattgttata
     9181 tataatagac cacttatccg aattgtgaCC CGGGGGCGCG CCgaaataaa ttgaattgaa
     9241 ttgaaatcga tagatcaatt tttttctttt ctctttcccc atcctttacg ctaaaataat
     9301 agtttatttt attttttgaa tattttttat ttatatacgt atatatagac tattatttat
     9361 cttttaatga ttattaagat ttttattaaa aaaaaattcg ctcctctttt aatgccttta
     9421 tgcagttttt ttttcccatt cgatatttct atgttcgggt tcagcgtatt ttaagtttaa
     9481 taactcgaaa attctgcgtt cgttaaagct GAGCTCacta gttctagagc ggccgccacc
     9541 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     9601 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacataCg
     9661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagCtaac tcacattaat
     9721 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10441 tagaagAaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
    11761 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
    11821 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
    11881 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
    11941 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
    12001 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
    12061 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
    12121 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
    12181 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
    12241 gaggcgcgtg taagttacag gcaagcgatc cgtctaagaa accattatta tcatgacatt
    12301 aacctataaa aataggcgta tcacgaggcc ctttcgtc  
//
