LOCUS       Exported                7136 bp ds-DNA     circular SYN 17-JAN-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Rebecca Wood
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2021 from SnapGene 4.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          891..893
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          894..938
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        312..330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    331..355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             370..392
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             399..1946
                     /codon_start=1
                     /product="Petunia hybrida DAD2 strigolactone receptor with 
                     cpGFP inserted after N180"
                     /label=DAD2cpGFP(N180)
                     /label=DAD2cpGFP(G164)
                     /label=DAD2cpGFP(G166)
                     /note="Petunia hybrida DAD2 SL receptor with cpGFP inserted
                     after G166"
                     /translation="MGQTLLDALNVRVVGSGERVLVLAHGFGTDQSAWNRILPFFLRDY
                     RVVLYDLVCAGSVNPDFFDFRRYTTLDPYVDDLLHILDALGIDCCAYVGHSVSAMIGIL
                     ASIRRPELFSKLILIGASPRFLNDEDYHGGFEQGEIEKVFSAMEANYEAWVNGFAPLAV
                     GADVPAAVREFSRTLFNASYNVFIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTP
                     IGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMV
                     SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTL
                     VTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNFNASMRPDITLFVSRTVFNSDMRGVLGLVKVPCH
                     IFQTARDHSVPASVATYLKNHLGGKNTVHWLNIEGHLPHLSAPTLLAQELRRALSHR"
     CDS             945..1676
                     /codon_start=1
                     /product="circularly permuted enhanced green fluorescent 
                     protein"
                     /label=cpGFP
                     /translation="YNVFIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNFN"
     terminator      1959..2006
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2181..2209)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2322..2426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2427..3287
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3458..4046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4232..4374
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4476..4667)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(5979..7061)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(7062..3)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
       61 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
      121 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
      181 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
      241 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
      301 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      361 agaaataatt ttgtttaact ttaagaagga gatataccat gggtcaaact ttactggacg
      421 ccttgaatgt gagggttgta ggcagcggag agagagtgtt ggtacttgct cacgggtttg
      481 gtaccgacca gtccgcttgg aataggattc tacccttttt ccttagggac tatagagttg
      541 tactatacga tttggtgtgt gcaggatcag ttaaccctga cttctttgat tttaggaggt
      601 acacaacact tgacccttat gtggatgatt tgctgcatat attagacgcg ctggggatag
      661 actgttgcgc ttatgttggt cattcagtat cagcaatgat cggcatattg gcgtctatac
      721 gtaggccgga attattttct aagctaattt tgataggcgc gagtcctagg tttctaaacg
      781 atgaagacta ccacggcggt ttcgagcaag gagaaatcga aaaggtcttt tccgcgatgg
      841 aggcaaacta tgaggcgtgg gtgaatggat tcgcgccttt ggcagtgggc gctgatgtac
      901 cggcagcggt aagggaattc tcccgtacac tgtttaacgc atcgtataac gtctttatca
      961 tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
     1021 acggcggcgt gcagctcgcc tatcactacc agcagaacac ccccatcggc gacggccccg
     1081 tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa actgagcaaa gaccccaacg
     1141 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1201 tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt
     1261 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1321 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1381 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1441 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1501 tgcccgaagg ctacattcag gagcgcacca tcttcttcaa ggacgacggc aactataaga
     1561 cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag ctgaagggca
     1621 tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac ttcaacgcgt
     1681 ccatgcgtcc agacataact ttgttcgtta gtaggacagt attcaacagc gatatgagag
     1741 gtgttttggg cttagtgaag gtcccttgtc atatctttca gacagctaga gatcattctg
     1801 tgcctgcgag tgtagccact tatctaaaga accacctagg cggtaaaaat acagtgcact
     1861 ggcttaacat agaaggtcac cttccacatc taagtgcgcc cactctttta gcacaagagc
     1921 ttaggcgtgc actgtctcat cgttaagctg agcaataact agcataaccc cttggggcct
     1981 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatcccgcaa
     2041 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
     2101 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
     2161 taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt
     2221 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2281 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2341 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2401 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2461 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2521 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2581 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2641 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     2701 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2761 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2821 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2881 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2941 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     3001 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3061 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3121 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3181 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3241 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3301 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3361 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3421 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3481 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3541 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3601 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3661 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3721 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3781 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3841 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3901 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3961 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4021 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4081 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4141 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4201 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     4261 tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc
     4321 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     4381 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4441 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4501 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     4561 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     4621 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     4681 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     4741 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     4801 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     4861 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     4921 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     4981 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     5041 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     5101 ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg
     5161 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     5221 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     5281 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
     5341 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
     5401 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta
     5461 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     5521 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     5581 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     5641 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
     5701 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     5761 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     5821 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
     5881 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
     5941 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6001 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6061 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
     6121 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     6181 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
     6241 tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc
     6301 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
     6361 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
     6421 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
     6481 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
     6541 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
     6601 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
     6661 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
     6721 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
     6781 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
     6841 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
     6901 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
     6961 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
     7021 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
     7081 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggt
//
