LOCUS       Exported               11229 bp ds-DNA     circular SYN 19-JAN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   wallv
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 19, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2830..6696
                     /codon_start=1
                     /label=SARS-CoV-2-S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
                     SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIA
                     YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL
                     LQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKP
                     SKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA
                     QYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSA
                     IGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEA
                     EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYH
                     LMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR
                     NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDL
                     GDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQGSGYIPEAPRDGQAYVR
                     KDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSAWSHP
                     QFEK"
     misc_feature    4669..4671
                     /label=D614G
     CDS             6463..6546
                     /codon_start=1
                     /label=T4 foldon
                     /translation="YIPEAPRDGQAYVRKDGEWVLLSTFLGR"
     CDS             6550..6573
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6577..6600
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6607..6630
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6670..6693
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6695..6719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6781..6799)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(6782..6799)
                     /label=SP6
     polyA_signal    6825..7049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7095..7523
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7537..7867
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7718..7853
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7934..8728
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    8783..9155
                     /label=pA
                     /note="SV40 polyA"
     polyA_signal    8902..9023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9072..9088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9096..9112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9120..9150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9165..9186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9474..10062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10233..11093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11094..11198)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcgtgtt ccttgtcctc ctgccgctgg tgtcatcgca gtgcgtgaac
     2881 ctgaccaccc ggactcagct gcctcctgct tacactaact ccttcactcg cggggtgtac
     2941 taccctgaca aagtgttccg gtcgtccgtg ctgcatagca cccaggacct gttccttccg
     3001 ttcttctcca acgtgacctg gttccacgcc attcacgtgt cggggaccaa cggcactaag
     3061 agattcgaca accccgtctt gcccttcaat gacggagtat acttcgcctc gacggaaaag
     3121 tccaacatca ttcggggttg gatcttcggc actaccctgg acagcaagac ccagtccctg
     3181 ctcattgtca acaacgccac caacgtcgtg atcaaagtct gcgagtttca gttttgtaac
     3241 gatccgttcc tcggcgtgta ctaccacaag aacaacaaga gctggatgga gtccgaattc
     3301 cgggtctact cctccgccaa caactgtacc ttcgaatacg tgtcccaacc tttcctcatg
     3361 gacctcgaag gaaaacaggg aaacttcaag aacttgagag agtttgtgtt caagaatatc
     3421 gatggctact tcaagatcta ctcgaagcat accccgatca acctcgtgcg ggacctacct
     3481 caagggttct ccgcacttga gccgctcgtg gacctcccca ttggcatcaa catcaccaga
     3541 ttccagactt tactggcact gcatagatcg tatctcacac ccggggactc ctcctccggg
     3601 tggacagctg gtgctgcggc ctactacgtg ggatatctcc agccccggac cttcttgctg
     3661 aagtacaacg agaacggcac catcacggat gccgtggact gtgcactgga ccccttgagt
     3721 gaaaccaagt gcacgctgaa gtccttcacc gtggaaaaag gcatctacca gaccagcaac
     3781 ttcagggtgc agcctactga atcaatcgtg cggttcccta acattaccaa tctgtgcccg
     3841 ttcggcgaag tgttcaacgc cactagattc gcctcggtgt acgcctggaa ccgaaagcgg
     3901 atcagcaact gcgtggccga ctactcagtg ctctacaact cggccagctt ctccaccttc
     3961 aagtgctacg gagtgtcgcc caccaagctg aacgatctgt gcttcaccaa cgtgtacgcg
     4021 gattccttcg tgatccgcgg agatgaagtc cgccaaatcg cccccggtca aaccggaaag
     4081 attgccgact acaactataa gctgccggac gactttaccg gatgcgtgat tgcctggaat
     4141 tccaacaatt tggattcaaa agtcggcggc aactacaact acctgtaccg cctgttccgg
     4201 aagtcaaacc tgaagccgtt cgaacgcgac atcagcaccg aaatctatca agcgggaagc
     4261 actccctgca acggggtgga gggcttcaac tgctacttcc cgctgcaaag ctacggattc
     4321 cagcctacca acggcgttgg ataccagcca tatcgcgtgg tcgtgctctc ctttgaactt
     4381 ctgcacgccc ctgccactgt gtgcggcccc aagaagtcca ctaacctcgt caagaataag
     4441 tgcgtgaatt tcaactttaa cggattgacc gggaccggag tgctcaccga gtccaacaag
     4501 aaattcctgc cgttccaaca gtttggacgg gacatcgccg atactacgga tgctgtgcgg
     4561 gacccacaga ctctggagat cctggacatt actccctgtt cctttggcgg ggtgtcggtc
     4621 atcacccctg gtactaacac cagcaaccag gtcgccgtgc tgtatcaggg ggtgaactgc
     4681 accgaagtgc ccgtggcgat ccacgccgac cagctgactc caacttggcg cgtgtactcg
     4741 actggttcga acgtgttcca gactcgggcc ggttgcctga tcggagcgga gcatgtcaac
     4801 aacagctacg agtgcgacat ccctattgga gccgggattt gcgccagcta ccagacccag
     4861 accaattcgc ctgggtcggc cagcagcgtg gcttcccaat cgatcatcgc ctacaccatg
     4921 tccctgggag ccgagaactc agtggcgtac tccaacaact cgattgccat ccccactaat
     4981 ttcacgatca gcgtgaccac cgagatcctg cctgtgtcca tgaccaagac ctccgtggac
     5041 tgcaccatgt acatctgcgg agactccacc gagtgctcca acctcctgct gcaatacggc
     5101 tcgttctgta cccaactgaa ccgagccctg actgggatcg ccgtggagca ggacaagaac
     5161 acacaggagg tgttcgcaca agtcaagcag atctacaaga ccccgcccat caaggatttc
     5221 ggagggttca atttctccca aattctgccg gacccttcca agccgtccaa gaggtcattc
     5281 attgaggatc tcctgttcaa caaggtcacc ctggccgatg ctggtttcat caagcagtac
     5341 ggagactgcc tgggggacat cgcagcccgc gatctgatct gcgcccaaaa gttcaacggc
     5401 ctgaccgtcc tgcctccgct tctgactgat gaaatgattg cccagtacac ctctgcgctg
     5461 ctggctggca ctatcacctc cggctggacc ttcggcgctg gagcagcact gcagattcca
     5521 tttgccatgc aaatggccta ccgcttcaac ggaatcggag tgactcagaa cgtcctgtac
     5581 gagaaccaga agctcatcgc caaccagttc aactccgcca ttgggaagat tcaggattcc
     5641 ctgtcgagca ctgcgtcagc cctgggaaag ctgcaggacg tcgtgaacca gaacgcacag
     5701 gccctgaaca cccttgtgaa gcagctgtcg tcgaacttcg gggcgatctc ctccgtgttg
     5761 aacgacatcc tgtcccggct ggacccgcct gaggcagaag tgcagatcga ccggctgatt
     5821 accggacgcc tccagtccct tcaaacttat gtgacccagc agctgattcg cgcggcggaa
     5881 attcgggcta gcgccaatct ggctgccacc aagatgagcg aatgcgtgct tggacagtcc
     5941 aagagagtgg acttttgtgg aaagggctac cacctgatga gcttccccca atccgcccca
     6001 cacggtgtcg tgtttctgca cgtgacctac gtgccggcgc aggaaaagaa cttcaccact
     6061 gcccctgcca tctgccacga tgggaaggcg cacttcccga gggagggagt gttcgtgtca
     6121 aacggaaccc actggttcgt gacccaaagg aacttctacg agccccagat tatcaccacc
     6181 gataacactt tcgtgagcgg caactgcgac gtggtcatcg gcatcgtgaa caacactgtg
     6241 tacgacccgc tgcaaccgga gctcgatagc tttaaggaag aactcgacaa gtactttaag
     6301 aatcatacgt cccccgatgt ggatctgggc gacattagcg gtatcaacgc gtctgtcgtg
     6361 aacattcaga aggagattga caggctcaac gaagtcgcca aaaacctgaa cgagagcctg
     6421 atagacttgc aggaactggg aaaatacgag cagggctccg gttacatccc cgaagctcca
     6481 cgggacggac aggcctacgt gcgcaaggac ggagaatggg tgctgctgag caccttcctc
     6541 gggcggtccc tggaggtgct gttccaaggc ccaggccacc atcatcatca ccaccaccac
     6601 tcggcttggt cacaccccca attcgaaaag ggcggtggtt ctggaggagg cggatccgga
     6661 ggatccgcgt ggagtcatcc gcagtttgaa aagtaatcaa ctttcttgta caaagttgtg
     6721 aaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggccctat
     6781 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     6841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7021 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     7081 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     7261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7441 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     7501 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7561 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7621 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7681 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7741 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7801 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7861 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     7921 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7981 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     8041 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     8101 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     8161 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     8221 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     8281 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     8341 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     8401 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     8461 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     8521 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     8581 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     8641 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     8701 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     8761 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8821 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8881 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8941 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9001 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9061 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9121 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9181 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9241 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9301 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9361 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9661 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9901 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11221 cctgacgtc
//
