LOCUS       Exported               11175 bp ds-DNA     circular SYN 20-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 19, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2830..6642
                     /codon_start=1
                     /label=SARS-CoV S-2P-3C-His8-Strep2x2
                     /translation="MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDE
                     IFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFG
                     STMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEY
                     ISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKL
                     PLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQN
                     PLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAW
                     ERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAP
                     GQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNV
                     PFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLST
                     DLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPC
                     AFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGC
                     LIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTI
                     AIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIA
                     AEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLAD
                     AGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFG
                     AGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKL
                     QDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTY
                     VTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVT
                     YVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGN
                     CDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEID
                     RLNEVAKNLNESLIDLQELGKYEQGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEV
                     LFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSAWSHPQFEK"
     CDS             6496..6519
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6523..6546
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6553..6576
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6616..6639
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6641..6665)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6727..6745)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(6728..6745)
                     /label=SP6
     polyA_signal    6771..6995
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7041..7469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7483..7813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7664..7799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7880..8674
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    8729..9101
                     /label=pA
                     /note="SV40 polyA"
     polyA_signal    8848..8969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9018..9034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9042..9058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9066..9096)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9111..9132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9420..10008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10179..11039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11040..11144)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcatctt cctcctgttc ctgaccctga cttcgggatc cgacctcgac
     2881 agatgtacca ccttcgatga cgtgcaggct cccaactata cccagcatac ttcctcgatg
     2941 cggggagtgt actaccccga tgaaatcttc aggagcgaca ctctgtacct gactcaagac
     3001 ctcttcctgc ccttctactc gaacgtgacc ggcttccaca ccatcaacca cactttcggc
     3061 aaccccgtca tcccgttcaa ggacggaatc tacttcgccg ccaccgagaa aagcaacgtc
     3121 gtgcgcggat gggtgtttgg atccaccatg aacaacaaga gccaaagcgt gatcatcatt
     3181 aacaactcaa ccaacgtcgt gatccgggcc tgcaatttcg agctctgcga caacccgttc
     3241 ttcgcggtgt ccaagccgat gggaactcag acccacacca tgatcttcga taacgccttc
     3301 aactgtacct tcgagtacat ttccgacgcg ttctccctcg acgtcagcga aaagtcggga
     3361 aacttcaagc accttcggga gttcgtgttc aagaacaagg atggctttct gtatgtgtac
     3421 aaagggtacc agcccatcga tgtggtccgg gatctgccga gcggcttcaa caccctgaag
     3481 ccgattttca agctgcctct tggtatcaac atcacgaact ttagggccat cctcaccgct
     3541 ttctcccctg cacaagacat ctggggtact tccgccgctg cctacttcgt gggctacctg
     3601 aaacccacca ccttcatgct gaaatacgac gaaaacggaa ccatcacaga tgccgtggat
     3661 tgcagccaga acccgctggc tgagctgaag tgctcagtga agtcatttga aattgataag
     3721 gggatctatc aaacatccaa cttcagagtg gtgccgtctg gcgatgtcgt ccgcttccct
     3781 aacatcacta atctgtgccc cttcggcgaa gtgttcaacg ccactaagtt ccctagcgtg
     3841 tacgcctggg agcggaagaa gatctcgaat tgcgtggccg actactctgt gctgtacaac
     3901 tccaccttct tctcgacctt caagtgctac ggagtgtccg cgactaagct gaacgatctc
     3961 tgcttttcca acgtctacgc ggacagcttt gtcgtgaaag gggacgatgt gcggcagatc
     4021 gcgccaggac agaccggagt gattgccgac tacaattaca agctgcctga cgacttcatg
     4081 ggttgcgtcc tcgcgtggaa cacccggaac atcgacgcca cctccaccgg aaactacaac
     4141 tacaagtata gatacctccg ccacggaaag ctccggccat tcgaaagaga catctcaaac
     4201 gtccctttct ccccggacgg aaagccttgt accccgcctg cgcttaactg ctactggccc
     4261 ctgaacgact acggcttcta caccactacc ggcattggct accagcccta ccgggtggtg
     4321 gtgctgtcct tcgagctcct gaacgctcca gccactgtct gcggtcctaa gttgtccact
     4381 gatctgatta agaaccaatg cgtgaacttc aacttcaacg ggctgaccgg aaccggagtc
     4441 ttgaccccat cgtccaagcg gttccagccg ttccagcaat tcggacggga cgtgtcagac
     4501 tttaccgata gcgtgcggga ccctaagacc agcgagatcc tggacatctc gccgtgcgcg
     4561 tttggtggag tgtccgtgat tacccccggc accaatgcct cctccgaagt ggccgtgctg
     4621 taccaggatg tgaactgcac cgatgtgtcc accgccatcc acgccgacca gttgaccccc
     4681 gcctggagaa tctactcgac cggaaacaac gtgttccaga ctcaggccgg ctgtctcatc
     4741 ggtgccgagc acgtggacac ctcctatgag tgtgacatcc ctatcggcgc gggaatctgc
     4801 gcgtcctacc atactgtctc gctgctgcgc agtacctcgc aaaaatccat cgtggcgtac
     4861 accatgagcc tcggagcgga ctcatccatc gcctactcga acaacaccat cgccatccct
     4921 accaacttct ccatctctat cactaccgag gtcatgcccg tgtccatggc caagacctcg
     4981 gtggactgca atatgtacat ctgtggggac agcaccgaat gcgcgaatct gctgttgcag
     5041 tacggctcct tctgtacaca actgaaccgc gcactgtcag gcattgccgc tgagcaagac
     5101 agaaacacgc gcgaagtgtt tgcccaagtg aagcagatgt acaaaacccc gactctgaag
     5161 tactttggag gattcaattt ctcgcaaatt ctgcccgatc cgctcaagcc caccaagagg
     5221 tccttcattg aggacctcct gtttaacaag gtcacgttgg cggatgccgg gttcatgaag
     5281 cagtacggag aatgcctggg agacatcaac gcccgcgacc tcatctgcgc ccaaaagttc
     5341 aatggcctga ccgtgctgcc tccgctcctc actgatgaca tgattgccgc ttataccgcc
     5401 gcactggtgt caggaactgc aactgccggc tggaccttcg gtgctggcgc tgcacttcaa
     5461 attccgttcg ccatgcaaat ggcctaccgg ttcaacggca ttggcgtgac tcagaacgtc
     5521 ctgtacgaga accaaaagca aatcgccaat cagttcaaca aggccatcag ccagatccag
     5581 gagtccctca ccaccacttc gaccgccctg ggaaagctgc aggacgtggt caaccagaac
     5641 gcgcaggccc tcaacaccct cgtgaagcag ctgagctcca acttcggcgc aatctcctcg
     5701 gtgctgaacg atattctttc acgcctggac cctccagagg ccgaggtgca aatcgacaga
     5761 ctgattactg gacggctgca gagcctgcag acctacgtga ctcagcaact gatcagagcc
     5821 gcggaaatcc gagccagcgc taacctcgct gcaaccaaga tgtctgaatg cgtgctgggg
     5881 cagtcgaagc gggtggactt ctgcggaaag gggtaccacc tgatgagctt cccacaagct
     5941 gccccacacg gagtggtctt tctgcacgtg acctacgtgc ctagccaaga gcgcaacttt
     6001 accaccgcac cggctatttg ccatgaaggg aaggcgtact tcccacgcga aggagtgttc
     6061 gtgtttaacg gcacctcctg gttcattact cagcgcaact tctttagccc ccaaattatc
     6121 accaccgaca acacctttgt ctccggaaac tgcgacgtgg tcatcgggat cataaacaac
     6181 actgtgtacg atcctctgca acccgagctg gatagtttca aggaagaact cgacaagtac
     6241 ttcaagaatc acacgtcccc ggatgtcgac ctgggcgata ttagcggtat caacgcctcc
     6301 gtggtgaaca ttcagaagga aatcgacagg ctgaacgaag tcgccaagaa cctgaacgaa
     6361 agcctgatcg accttcagga acttgggaaa tacgaacagg gcagcggtta cattcccgaa
     6421 gccccgagag atggacaggc ctacgtgcgc aaggacggag aatgggtgct gctgtccact
     6481 tttctgggtc ggtccctcga ggtgctgttc cagggtccgg gtcaccatca ccaccaccat
     6541 catcactccg catggagcca cccgcagttc gaaaagggag gaggatccgg aggaggaggt
     6601 tcagggggat ccgcctggag ccatccccag ttcgagaagt aatcaacttt cttgtacaaa
     6661 gttgtgaaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg
     6721 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc
     6781 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6841 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6901 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6961 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     7021 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7081 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7141 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat
     7201 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7261 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7321 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7381 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga
     7441 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7501 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7561 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7621 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7681 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7741 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7801 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     7861 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7921 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7981 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     8041 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     8101 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     8161 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     8221 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     8281 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     8341 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     8401 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     8461 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     8521 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     8581 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     8641 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     8701 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     8761 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     8821 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     8881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8941 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     9001 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9061 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9121 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9181 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9241 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9601 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9841 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10081 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10141 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10201 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10261 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10321 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10381 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10441 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10501 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10561 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10621 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10681 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10741 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10801 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10861 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10921 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10981 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11041 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11101 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11161 gtgccacctg acgtc
//
